Isolation and characterization of mutants of thiophene synthesis in Tagetes erecta
Two mutants of Tagetes erecta displaying aberrant thiophene composition were identified by screening more than 300 plants from a mutagenized M2 population using high-performance liquid chromatography analysis of root extracts. Both mutants, which may have originated from the same mutational event, c...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1995-03, Vol.107 (3), p.807-814 |
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| container_title | Plant physiology (Bethesda) |
| container_volume | 107 |
| creator | Jacobs, J.J. (University of Nijmegen, Nijmegen, The Netherlands.) Arroo, R.R Koning, E.A. de Klunder, A.J Croes, A.F Wullems, G.J |
| description | Two mutants of Tagetes erecta displaying aberrant thiophene composition were identified by screening more than 300 plants from a mutagenized M2 population using high-performance liquid chromatography analysis of root extracts. Both mutants, which may have originated from the same mutational event, contained high amounts of the C13 monothiophene 2-(but-3-1-ynyl)-5-(penta-1,3-diynyl)- thiophene that was previously not found in T. erecta and also high amounts of two C13 bithienyls that were absent or present at low concentrations in the wild type. The mutant phenotype was also expressed in 21 Agrobacterium rhizogenes transformed root clones derived from both mutants. Feeding experiments with root cultures derived from one mutant and from the wild type indicated that the monothiophene accumulating in the mutant is the common precursor for all bithienyl thiophenes in wild-type and mutant Tagetes erecta. These experiments also showed that one mutant is deficient in demethylation of the monothiophene |
| doi_str_mv | 10.1104/pp.107.3.807 |
| format | Article |
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(University of Nijmegen, Nijmegen, The Netherlands.) ; Arroo, R.R ; Koning, E.A. de ; Klunder, A.J ; Croes, A.F ; Wullems, G.J</creator><creatorcontrib>Jacobs, J.J. (University of Nijmegen, Nijmegen, The Netherlands.) ; Arroo, R.R ; Koning, E.A. de ; Klunder, A.J ; Croes, A.F ; Wullems, G.J</creatorcontrib><description>Two mutants of Tagetes erecta displaying aberrant thiophene composition were identified by screening more than 300 plants from a mutagenized M2 population using high-performance liquid chromatography analysis of root extracts. Both mutants, which may have originated from the same mutational event, contained high amounts of the C13 monothiophene 2-(but-3-1-ynyl)-5-(penta-1,3-diynyl)- thiophene that was previously not found in T. erecta and also high amounts of two C13 bithienyls that were absent or present at low concentrations in the wild type. The mutant phenotype was also expressed in 21 Agrobacterium rhizogenes transformed root clones derived from both mutants. Feeding experiments with root cultures derived from one mutant and from the wild type indicated that the monothiophene accumulating in the mutant is the common precursor for all bithienyl thiophenes in wild-type and mutant Tagetes erecta. These experiments also showed that one mutant is deficient in demethylation of the monothiophene</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.107.3.807</identifier><identifier>PMID: 12228405</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>AGROBACTERIUM RHIZOGENES ; Agronomy. Soil science and plant productions ; ANALISIS CUANTITATIVO ; ANALYSE QUANTITATIVE ; Biochemistry and Enzymology ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; CLONE ; CLONES ; COMPOSE ORGANOSOUFRE ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; COMPUESTO ORGANICO DEL AZUFRE ; Cytochromes ; Economic plant physiology ; EMS ; Enzymes ; ESTRUCTURA QUIMICA ; Flowers ; Fundamental and applied biological sciences. Psychology ; Genetic mutation ; HIPOCOTILOS ; HYPOCOTYLE ; Hypocotyls ; Metabolism ; Metabolism. Physicochemical requirements ; MUTANT ; MUTANTES ; Nitrogen metabolism and other ones (excepting carbon metabolism) ; Nutrition. Photosynthesis. Respiration. Metabolism ; Plant physiology and development ; Plant roots ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants ; RACINE ; RAICES ; Seeds ; STRUCTURE CHIMIQUE ; TAGETES ; Tagetes erecta ; Thiophenes ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; VARIACION GENETICA ; VARIATION GENETIQUE</subject><ispartof>Plant physiology (Bethesda), 1995-03, Vol.107 (3), p.807-814</ispartof><rights>Copyright 1995 American Society of Plant Physiologists</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-f526017520b43a8839fd5e76672b89a535f29636796e7e772d442a262af9885f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4276394$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4276394$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3588397$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12228405$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jacobs, J.J. (University of Nijmegen, Nijmegen, The Netherlands.)</creatorcontrib><creatorcontrib>Arroo, R.R</creatorcontrib><creatorcontrib>Koning, E.A. de</creatorcontrib><creatorcontrib>Klunder, A.J</creatorcontrib><creatorcontrib>Croes, A.F</creatorcontrib><creatorcontrib>Wullems, G.J</creatorcontrib><title>Isolation and characterization of mutants of thiophene synthesis in Tagetes erecta</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Two mutants of Tagetes erecta displaying aberrant thiophene composition were identified by screening more than 300 plants from a mutagenized M2 population using high-performance liquid chromatography analysis of root extracts. Both mutants, which may have originated from the same mutational event, contained high amounts of the C13 monothiophene 2-(but-3-1-ynyl)-5-(penta-1,3-diynyl)- thiophene that was previously not found in T. erecta and also high amounts of two C13 bithienyls that were absent or present at low concentrations in the wild type. The mutant phenotype was also expressed in 21 Agrobacterium rhizogenes transformed root clones derived from both mutants. Feeding experiments with root cultures derived from one mutant and from the wild type indicated that the monothiophene accumulating in the mutant is the common precursor for all bithienyl thiophenes in wild-type and mutant Tagetes erecta. These experiments also showed that one mutant is deficient in demethylation of the monothiophene</description><subject>AGROBACTERIUM RHIZOGENES</subject><subject>Agronomy. Soil science and plant productions</subject><subject>ANALISIS CUANTITATIVO</subject><subject>ANALYSE QUANTITATIVE</subject><subject>Biochemistry and Enzymology</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>CLONE</subject><subject>CLONES</subject><subject>COMPOSE ORGANOSOUFRE</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>COMPUESTO ORGANICO DEL AZUFRE</subject><subject>Cytochromes</subject><subject>Economic plant physiology</subject><subject>EMS</subject><subject>Enzymes</subject><subject>ESTRUCTURA QUIMICA</subject><subject>Flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic mutation</subject><subject>HIPOCOTILOS</subject><subject>HYPOCOTYLE</subject><subject>Hypocotyls</subject><subject>Metabolism</subject><subject>Metabolism. Physicochemical requirements</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Nitrogen metabolism and other ones (excepting carbon metabolism)</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants</subject><subject>RACINE</subject><subject>RAICES</subject><subject>Seeds</subject><subject>STRUCTURE CHIMIQUE</subject><subject>TAGETES</subject><subject>Tagetes erecta</subject><subject>Thiophenes</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNpVkc9rFDEUx4NY7Fq9eRKROUhP3TU_J8mhBylqCwVB23N4O5vspMwmY5IttH-9GWZZ9ZQv-X7e9z3eQ-gdwStCMP88jiuC5YqtFJYv0IIIRpdUcPUSLTCuGiulT9HrnB8wxoQR_gqdEkqp4lgs0M-bHAcoPoYGwqbpekjQFZv88_wZXbPbFwglT7L0Po69DbbJT6H0Nvvc-NDcwdYWmxubbFfgDTpxMGT79vCeoftvX--urpe3P77fXH25XXaCyLJ0graYSEHxmjNQimm3EVa2raRrpUEw4ahuWSt1a6WVkm44p0BbCk4rJRw7Q5dz7rhf7-yms6EkGMyY_A7Sk4ngzf9O8L3ZxkdDhCRa1vrzQ32Kv_c2F7PzubPDAMHGfa5Y7a44r-DFDHYp5pysO_Yg2Ew3MONYpTTM1BtU_OO_c_2FD0uvwKcDALmDwSUInc9HjolpGVPOhxl7yCWmo82pbJmepno_2w6igW2qCfe_tORYScL-ABRfoTQ</recordid><startdate>19950301</startdate><enddate>19950301</enddate><creator>Jacobs, J.J. (University of Nijmegen, Nijmegen, The Netherlands.)</creator><creator>Arroo, R.R</creator><creator>Koning, E.A. de</creator><creator>Klunder, A.J</creator><creator>Croes, A.F</creator><creator>Wullems, G.J</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19950301</creationdate><title>Isolation and characterization of mutants of thiophene synthesis in Tagetes erecta</title><author>Jacobs, J.J. (University of Nijmegen, Nijmegen, The Netherlands.) ; Arroo, R.R ; Koning, E.A. de ; Klunder, A.J ; Croes, A.F ; Wullems, G.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-f526017520b43a8839fd5e76672b89a535f29636796e7e772d442a262af9885f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AGROBACTERIUM RHIZOGENES</topic><topic>Agronomy. Soil science and plant productions</topic><topic>ANALISIS CUANTITATIVO</topic><topic>ANALYSE QUANTITATIVE</topic><topic>Biochemistry and Enzymology</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>CLONE</topic><topic>CLONES</topic><topic>COMPOSE ORGANOSOUFRE</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>COMPUESTO ORGANICO DEL AZUFRE</topic><topic>Cytochromes</topic><topic>Economic plant physiology</topic><topic>EMS</topic><topic>Enzymes</topic><topic>ESTRUCTURA QUIMICA</topic><topic>Flowers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic mutation</topic><topic>HIPOCOTILOS</topic><topic>HYPOCOTYLE</topic><topic>Hypocotyls</topic><topic>Metabolism</topic><topic>Metabolism. Physicochemical requirements</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Nitrogen metabolism and other ones (excepting carbon metabolism)</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants</topic><topic>RACINE</topic><topic>RAICES</topic><topic>Seeds</topic><topic>STRUCTURE CHIMIQUE</topic><topic>TAGETES</topic><topic>Tagetes erecta</topic><topic>Thiophenes</topic><topic>TRANSFERENCIA DE GENES</topic><topic>TRANSFERT DE GENE</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jacobs, J.J. (University of Nijmegen, Nijmegen, The Netherlands.)</creatorcontrib><creatorcontrib>Arroo, R.R</creatorcontrib><creatorcontrib>Koning, E.A. de</creatorcontrib><creatorcontrib>Klunder, A.J</creatorcontrib><creatorcontrib>Croes, A.F</creatorcontrib><creatorcontrib>Wullems, G.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jacobs, J.J. (University of Nijmegen, Nijmegen, The Netherlands.)</au><au>Arroo, R.R</au><au>Koning, E.A. de</au><au>Klunder, A.J</au><au>Croes, A.F</au><au>Wullems, G.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of mutants of thiophene synthesis in Tagetes erecta</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1995-03-01</date><risdate>1995</risdate><volume>107</volume><issue>3</issue><spage>807</spage><epage>814</epage><pages>807-814</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Two mutants of Tagetes erecta displaying aberrant thiophene composition were identified by screening more than 300 plants from a mutagenized M2 population using high-performance liquid chromatography analysis of root extracts. Both mutants, which may have originated from the same mutational event, contained high amounts of the C13 monothiophene 2-(but-3-1-ynyl)-5-(penta-1,3-diynyl)- thiophene that was previously not found in T. erecta and also high amounts of two C13 bithienyls that were absent or present at low concentrations in the wild type. The mutant phenotype was also expressed in 21 Agrobacterium rhizogenes transformed root clones derived from both mutants. Feeding experiments with root cultures derived from one mutant and from the wild type indicated that the monothiophene accumulating in the mutant is the common precursor for all bithienyl thiophenes in wild-type and mutant Tagetes erecta. These experiments also showed that one mutant is deficient in demethylation of the monothiophene</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>12228405</pmid><doi>10.1104/pp.107.3.807</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1995-03, Vol.107 (3), p.807-814 |
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| language | eng |
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| source | Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | AGROBACTERIUM RHIZOGENES Agronomy. Soil science and plant productions ANALISIS CUANTITATIVO ANALYSE QUANTITATIVE Biochemistry and Enzymology Biological and medical sciences BIOSINTESIS BIOSYNTHESE CLONE CLONES COMPOSE ORGANOSOUFRE COMPOSICION QUIMICA COMPOSITION CHIMIQUE COMPUESTO ORGANICO DEL AZUFRE Cytochromes Economic plant physiology EMS Enzymes ESTRUCTURA QUIMICA Flowers Fundamental and applied biological sciences. Psychology Genetic mutation HIPOCOTILOS HYPOCOTYLE Hypocotyls Metabolism Metabolism. Physicochemical requirements MUTANT MUTANTES Nitrogen metabolism and other ones (excepting carbon metabolism) Nutrition. Photosynthesis. Respiration. Metabolism Plant physiology and development Plant roots PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants RACINE RAICES Seeds STRUCTURE CHIMIQUE TAGETES Tagetes erecta Thiophenes TRANSFERENCIA DE GENES TRANSFERT DE GENE VARIACION GENETICA VARIATION GENETIQUE |
| title | Isolation and characterization of mutants of thiophene synthesis in Tagetes erecta |
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