Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters

Ghrelin is a newly identified gastric peptide hormone that has various important functions, including growth‐hormone release and appetite stimulation. Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghreli...

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Veröffentlicht in:Journal of anatomy 2004-09, Vol.205 (3), p.239-246
Hauptverfasser: Yabuki, Akira, Ojima, Toshimichi, Kojima, Masayasu, Nishi, Yoshihiro, Mifune, Hiroharu, Matsumoto, Mitsuharu, Kamimura, Ryozo, Masuyama, Taku, Suzuki, Syusaku
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container_issue 3
container_start_page 239
container_title Journal of anatomy
container_volume 205
creator Yabuki, Akira
Ojima, Toshimichi
Kojima, Masayasu
Nishi, Yoshihiro
Mifune, Hiroharu
Matsumoto, Mitsuharu
Kamimura, Ryozo
Masuyama, Taku
Suzuki, Syusaku
description Ghrelin is a newly identified gastric peptide hormone that has various important functions, including growth‐hormone release and appetite stimulation. Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. In conclusion, gastric ghrelin cells in mice and hamsters are characterized by X‐type endocrine cells, as has been observed in rats. However, the data indicated that gastric ghrelin production was lower in hamster than in mouse or rat.
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Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. In conclusion, gastric ghrelin cells in mice and hamsters are characterized by X‐type endocrine cells, as has been observed in rats. 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Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. In conclusion, gastric ghrelin cells in mice and hamsters are characterized by X‐type endocrine cells, as has been observed in rats. However, the data indicated that gastric ghrelin production was lower in hamster than in mouse or rat.</description><subject>Animals</subject><subject>Cricetinae</subject><subject>Gastric Fundus - chemistry</subject><subject>Gastric Fundus - cytology</subject><subject>Ghrelin</subject><subject>hamster</subject><subject>Male</subject><subject>Mesocricetus</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Microscopy, Immunoelectron</subject><subject>mouse</subject><subject>Original</subject><subject>Peptide Hormones - analysis</subject><subject>Radioimmunoassay - methods</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Species Specificity</subject><subject>stomach</subject><issn>0021-8782</issn><issn>1469-7580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1vEzEQhi0EoqHwF5BPnNjgsb3rtYSQqggKqFIvcMWaeGcTR_sR7E1p-fX1NlGBG754RvPO4_G8jHEQS8jn3W4phISiNrVcSiF0TpWC5e0TtgBd2cKUtXjKFo-iM_YipZ0QoITVz9kZlMpYK-2C_VhtMaKfKIbfOIVx4Dg0PO3JB0q8CW1LkQaf4zDwDaYpBs8320hdzj11XeJtHHveB09vecQpPQC22KfMTC_Zsxa7RK9O9zn7_unjt9Xn4ur68svq4qrwpSqhgFJDhd6jqBUZ0gq9tC3IWjcCUXqoagvWiPW60dpIMLksZGlNUzYGGq_O2Ycjd39Y99R4GqaIndvH0GO8cyMG929lCFu3GW8clAaUhgx4cwLE8eeB0uT6kOb_4UDjIbkqT6B0JbOwPgp9HFOK1D4-AsLN5ridm_fu5r272Rz3YI67za2v_x7yT-PJjSx4fxT8Ch3d_TfYfb2-yIG6B7tnnt8</recordid><startdate>200409</startdate><enddate>200409</enddate><creator>Yabuki, Akira</creator><creator>Ojima, Toshimichi</creator><creator>Kojima, Masayasu</creator><creator>Nishi, Yoshihiro</creator><creator>Mifune, Hiroharu</creator><creator>Matsumoto, Mitsuharu</creator><creator>Kamimura, Ryozo</creator><creator>Masuyama, Taku</creator><creator>Suzuki, Syusaku</creator><general>Blackwell Science Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200409</creationdate><title>Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters</title><author>Yabuki, Akira ; 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Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. 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subjects Animals
Cricetinae
Gastric Fundus - chemistry
Gastric Fundus - cytology
Ghrelin
hamster
Male
Mesocricetus
Mice
Mice, Inbred ICR
Microscopy, Immunoelectron
mouse
Original
Peptide Hormones - analysis
Radioimmunoassay - methods
rat
Rats
Rats, Wistar
Species Specificity
stomach
title Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters
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