Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters
Ghrelin is a newly identified gastric peptide hormone that has various important functions, including growth‐hormone release and appetite stimulation. Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghreli...
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Veröffentlicht in: | Journal of anatomy 2004-09, Vol.205 (3), p.239-246 |
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creator | Yabuki, Akira Ojima, Toshimichi Kojima, Masayasu Nishi, Yoshihiro Mifune, Hiroharu Matsumoto, Mitsuharu Kamimura, Ryozo Masuyama, Taku Suzuki, Syusaku |
description | Ghrelin is a newly identified gastric peptide hormone that has various important functions, including growth‐hormone release and appetite stimulation. Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. In conclusion, gastric ghrelin cells in mice and hamsters are characterized by X‐type endocrine cells, as has been observed in rats. However, the data indicated that gastric ghrelin production was lower in hamster than in mouse or rat. |
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Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. In conclusion, gastric ghrelin cells in mice and hamsters are characterized by X‐type endocrine cells, as has been observed in rats. However, the data indicated that gastric ghrelin production was lower in hamster than in mouse or rat.</description><identifier>ISSN: 0021-8782</identifier><identifier>EISSN: 1469-7580</identifier><identifier>DOI: 10.1111/j.0021-8782.2004.00331.x</identifier><identifier>PMID: 15379929</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Cricetinae ; Gastric Fundus - chemistry ; Gastric Fundus - cytology ; Ghrelin ; hamster ; Male ; Mesocricetus ; Mice ; Mice, Inbred ICR ; Microscopy, Immunoelectron ; mouse ; Original ; Peptide Hormones - analysis ; Radioimmunoassay - methods ; rat ; Rats ; Rats, Wistar ; Species Specificity ; stomach</subject><ispartof>Journal of anatomy, 2004-09, Vol.205 (3), p.239-246</ispartof><rights>Anatomical Society of Great Britain and Ireland 2004 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5351-15416acca083e7e43ac29f1284d0aa2c16891970bbd447217c2902597d5d71dc3</citedby><cites>FETCH-LOGICAL-c5351-15416acca083e7e43ac29f1284d0aa2c16891970bbd447217c2902597d5d71dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1571341/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1571341/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27923,27924,45573,45574,46408,46832,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15379929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yabuki, Akira</creatorcontrib><creatorcontrib>Ojima, Toshimichi</creatorcontrib><creatorcontrib>Kojima, Masayasu</creatorcontrib><creatorcontrib>Nishi, Yoshihiro</creatorcontrib><creatorcontrib>Mifune, Hiroharu</creatorcontrib><creatorcontrib>Matsumoto, Mitsuharu</creatorcontrib><creatorcontrib>Kamimura, Ryozo</creatorcontrib><creatorcontrib>Masuyama, Taku</creatorcontrib><creatorcontrib>Suzuki, Syusaku</creatorcontrib><title>Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters</title><title>Journal of anatomy</title><addtitle>J Anat</addtitle><description>Ghrelin is a newly identified gastric peptide hormone that has various important functions, including growth‐hormone release and appetite stimulation. Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. In conclusion, gastric ghrelin cells in mice and hamsters are characterized by X‐type endocrine cells, as has been observed in rats. However, the data indicated that gastric ghrelin production was lower in hamster than in mouse or rat.</description><subject>Animals</subject><subject>Cricetinae</subject><subject>Gastric Fundus - chemistry</subject><subject>Gastric Fundus - cytology</subject><subject>Ghrelin</subject><subject>hamster</subject><subject>Male</subject><subject>Mesocricetus</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Microscopy, Immunoelectron</subject><subject>mouse</subject><subject>Original</subject><subject>Peptide Hormones - analysis</subject><subject>Radioimmunoassay - methods</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Species Specificity</subject><subject>stomach</subject><issn>0021-8782</issn><issn>1469-7580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1vEzEQhi0EoqHwF5BPnNjgsb3rtYSQqggKqFIvcMWaeGcTR_sR7E1p-fX1NlGBG754RvPO4_G8jHEQS8jn3W4phISiNrVcSiF0TpWC5e0TtgBd2cKUtXjKFo-iM_YipZ0QoITVz9kZlMpYK-2C_VhtMaKfKIbfOIVx4Dg0PO3JB0q8CW1LkQaf4zDwDaYpBs8320hdzj11XeJtHHveB09vecQpPQC22KfMTC_Zsxa7RK9O9zn7_unjt9Xn4ur68svq4qrwpSqhgFJDhd6jqBUZ0gq9tC3IWjcCUXqoagvWiPW60dpIMLksZGlNUzYGGq_O2Ycjd39Y99R4GqaIndvH0GO8cyMG929lCFu3GW8clAaUhgx4cwLE8eeB0uT6kOb_4UDjIbkqT6B0JbOwPgp9HFOK1D4-AsLN5ridm_fu5r272Rz3YI67za2v_x7yT-PJjSx4fxT8Ch3d_TfYfb2-yIG6B7tnnt8</recordid><startdate>200409</startdate><enddate>200409</enddate><creator>Yabuki, Akira</creator><creator>Ojima, Toshimichi</creator><creator>Kojima, Masayasu</creator><creator>Nishi, Yoshihiro</creator><creator>Mifune, Hiroharu</creator><creator>Matsumoto, Mitsuharu</creator><creator>Kamimura, Ryozo</creator><creator>Masuyama, Taku</creator><creator>Suzuki, Syusaku</creator><general>Blackwell Science Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200409</creationdate><title>Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters</title><author>Yabuki, Akira ; Ojima, Toshimichi ; Kojima, Masayasu ; Nishi, Yoshihiro ; Mifune, Hiroharu ; Matsumoto, Mitsuharu ; Kamimura, Ryozo ; Masuyama, Taku ; Suzuki, Syusaku</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5351-15416acca083e7e43ac29f1284d0aa2c16891970bbd447217c2902597d5d71dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Cricetinae</topic><topic>Gastric Fundus - chemistry</topic><topic>Gastric Fundus - cytology</topic><topic>Ghrelin</topic><topic>hamster</topic><topic>Male</topic><topic>Mesocricetus</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Microscopy, Immunoelectron</topic><topic>mouse</topic><topic>Original</topic><topic>Peptide Hormones - analysis</topic><topic>Radioimmunoassay - methods</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Species Specificity</topic><topic>stomach</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yabuki, Akira</creatorcontrib><creatorcontrib>Ojima, Toshimichi</creatorcontrib><creatorcontrib>Kojima, Masayasu</creatorcontrib><creatorcontrib>Nishi, Yoshihiro</creatorcontrib><creatorcontrib>Mifune, Hiroharu</creatorcontrib><creatorcontrib>Matsumoto, Mitsuharu</creatorcontrib><creatorcontrib>Kamimura, Ryozo</creatorcontrib><creatorcontrib>Masuyama, Taku</creatorcontrib><creatorcontrib>Suzuki, Syusaku</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of anatomy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yabuki, Akira</au><au>Ojima, Toshimichi</au><au>Kojima, Masayasu</au><au>Nishi, Yoshihiro</au><au>Mifune, Hiroharu</au><au>Matsumoto, Mitsuharu</au><au>Kamimura, Ryozo</au><au>Masuyama, Taku</au><au>Suzuki, Syusaku</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters</atitle><jtitle>Journal of anatomy</jtitle><addtitle>J Anat</addtitle><date>2004-09</date><risdate>2004</risdate><volume>205</volume><issue>3</issue><spage>239</spage><epage>246</epage><pages>239-246</pages><issn>0021-8782</issn><eissn>1469-7580</eissn><abstract>Ghrelin is a newly identified gastric peptide hormone that has various important functions, including growth‐hormone release and appetite stimulation. Ghrelin‐immunoreactive cells (ghrelin cells) are characterized by X‐type endocrine cells in the rat stomach. In the present study, we analysed ghrelin cells in fundi of stomach from ICR mice and Syrian hamsters immunohistochemically, immunoelectron microscopically and morphometrically, and compared the results with those from Wistar rats. Immunohistochemistry revealed that ghrelin cells were sparsely distributed in the proper gastric glands in all species. The number of ghrelin cells per unit area in hamsters was significantly lower than that in rats. Immunoelectron microscopy detected ghrelin immunolabelling in granules in the X‐type endocrine cells. However, the diameter of granules in the hamsters was significantly smaller than that in the mice and rats. Gastric ghrelin contents were determined by radioimmunoassay, and levels in the hamsters were significantly lower than those in mice and rats. The results from mice were identical to those from rats. In conclusion, gastric ghrelin cells in mice and hamsters are characterized by X‐type endocrine cells, as has been observed in rats. However, the data indicated that gastric ghrelin production was lower in hamster than in mouse or rat.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15379929</pmid><doi>10.1111/j.0021-8782.2004.00331.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Cricetinae Gastric Fundus - chemistry Gastric Fundus - cytology Ghrelin hamster Male Mesocricetus Mice Mice, Inbred ICR Microscopy, Immunoelectron mouse Original Peptide Hormones - analysis Radioimmunoassay - methods rat Rats Rats, Wistar Species Specificity stomach |
title | Characterization and species differences in gastric ghrelin cells from mice, rats and hamsters |
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