Recombinant human alpha‐2a interferon promotes an atypical process of mast cell secretion with ultrastructural features suggestive for piecemeal degranulation
Purified mast cells (MC), isolated from the rat peritoneum, were stimulated in vitro with recombinant human IFN‐α 2a (rhIFN‐α 2a) and studied ultrastructurally. Quantitative determination of histamine release was also performed. The following ultrastructural features were observed: (1) dilation of s...
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| Veröffentlicht in: | Journal of anatomy 2002-12, Vol.201 (6), p.507-512 |
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| creator | Crivellato, Enrico Candussio, Luigi Mallardi, Franco Ribatti, Domenico |
| description | Purified mast cells (MC), isolated from the rat peritoneum, were stimulated in vitro with recombinant human IFN‐α 2a (rhIFN‐α 2a) and studied ultrastructurally. Quantitative determination of histamine release was also performed. The following ultrastructural features were observed: (1) dilation of single granules, leading to the formation of cytoplasmic cavities filled with dissolved or eroded matrices; (2) induction of partially empty, non‐fused granule containers close to unaltered resting granules, a process very suggestive of piecemeal degranulation; (3) focal exocytosis, characterized by opening of single granules to the cell exterior and/or fusion of a few granules into small secretory channels. Histamine release was slightly increased in rhIFN‐α 2a‐treated MC, although not to significant levels. These results indicate that rhIFN‐α 2a induces a characteristic pattern of degranulation in rat peritoneal MC and that a small proportion of rhIFN‐α 2a‐stimulated MC shows ultrastructural features suggestive for piecemeal degranulation. |
| doi_str_mv | 10.1046/j.1469-7580.2002.00116.x |
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Quantitative determination of histamine release was also performed. The following ultrastructural features were observed: (1) dilation of single granules, leading to the formation of cytoplasmic cavities filled with dissolved or eroded matrices; (2) induction of partially empty, non‐fused granule containers close to unaltered resting granules, a process very suggestive of piecemeal degranulation; (3) focal exocytosis, characterized by opening of single granules to the cell exterior and/or fusion of a few granules into small secretory channels. Histamine release was slightly increased in rhIFN‐α 2a‐treated MC, although not to significant levels. These results indicate that rhIFN‐α 2a induces a characteristic pattern of degranulation in rat peritoneal MC and that a small proportion of rhIFN‐α 2a‐stimulated MC shows ultrastructural features suggestive for piecemeal degranulation.</description><identifier>ISSN: 0021-8782</identifier><identifier>EISSN: 1469-7580</identifier><identifier>DOI: 10.1046/j.1469-7580.2002.00116.x</identifier><identifier>PMID: 12489762</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; atypical degranulation ; Brief Communications ; Cell Degranulation ; Cells, Cultured ; Histamine Release ; Humans ; interferon ; Interferon alpha-2 ; Interferon-alpha - pharmacology ; mast cell ; Mast Cells - drug effects ; Mast Cells - physiology ; Mast Cells - ultrastructure ; Microscopy, Electron ; Peritoneum ; piecemeal degranulation ; rat ; Rats ; Rats, Wistar ; Recombinant Proteins ; Stimulation, Chemical</subject><ispartof>Journal of anatomy, 2002-12, Vol.201 (6), p.507-512</ispartof><rights>Anatomical Society of Great Britain and Ireland 2002 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4716-276c6a39b9e652295967a5571334ad064fe8ea6b9837ab37a7250bf443da4ff23</citedby><cites>FETCH-LOGICAL-c4716-276c6a39b9e652295967a5571334ad064fe8ea6b9837ab37a7250bf443da4ff23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1570992/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1570992/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12489762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Crivellato, Enrico</creatorcontrib><creatorcontrib>Candussio, Luigi</creatorcontrib><creatorcontrib>Mallardi, Franco</creatorcontrib><creatorcontrib>Ribatti, Domenico</creatorcontrib><title>Recombinant human alpha‐2a interferon promotes an atypical process of mast cell secretion with ultrastructural features suggestive for piecemeal degranulation</title><title>Journal of anatomy</title><addtitle>J Anat</addtitle><description>Purified mast cells (MC), isolated from the rat peritoneum, were stimulated in vitro with recombinant human IFN‐α 2a (rhIFN‐α 2a) and studied ultrastructurally. Quantitative determination of histamine release was also performed. The following ultrastructural features were observed: (1) dilation of single granules, leading to the formation of cytoplasmic cavities filled with dissolved or eroded matrices; (2) induction of partially empty, non‐fused granule containers close to unaltered resting granules, a process very suggestive of piecemeal degranulation; (3) focal exocytosis, characterized by opening of single granules to the cell exterior and/or fusion of a few granules into small secretory channels. Histamine release was slightly increased in rhIFN‐α 2a‐treated MC, although not to significant levels. These results indicate that rhIFN‐α 2a induces a characteristic pattern of degranulation in rat peritoneal MC and that a small proportion of rhIFN‐α 2a‐stimulated MC shows ultrastructural features suggestive for piecemeal degranulation.</description><subject>Animals</subject><subject>atypical degranulation</subject><subject>Brief Communications</subject><subject>Cell Degranulation</subject><subject>Cells, Cultured</subject><subject>Histamine Release</subject><subject>Humans</subject><subject>interferon</subject><subject>Interferon alpha-2</subject><subject>Interferon-alpha - pharmacology</subject><subject>mast cell</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - physiology</subject><subject>Mast Cells - ultrastructure</subject><subject>Microscopy, Electron</subject><subject>Peritoneum</subject><subject>piecemeal degranulation</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recombinant Proteins</subject><subject>Stimulation, Chemical</subject><issn>0021-8782</issn><issn>1469-7580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2OFCEUhYnROO3oKxhW7roEioIiMSaTib-ZZBKja0LRl246VUUJ1Mz0zkfwEXw2n0TK7oy6c0G44Zx7uPAhhCmpKOHi5b6iXKi1bFpSMUJYRQilorp7gFb3wkO0Kgpdt7JlZ-hJSvtiqonij9EZZbxVUrAV-vEJbBg6P5ox4908mBGbftqZn9--M4P9mCE6iGHEUwxDyJDw4siHyVvTL4cWUsLB4cGkjC30PU5gI2Rfem593uG5z7FocbZ5jqXHgSlFCUrzdgsp-xvALkQ8ebAwQHFsYBvNOPdmCXmKHjnTJ3h22s_Rl7dvPl--X19dv_tweXG1tlxSsWZSWGFq1SkQDWOqUUKappG0rrnZEMEdtGBEp9pamq4syRrSOc7rjeHOsfocvT7mTnM3wMbCWMbu9RT9YOJBB-P1v8rod3obbjRtJFFqCXhxCojh61wepgeflg8xI4Q5ackKiLoRxdgejTaGlCK4-0so0QtevdcLRb1Q1Ate_Ruvviutz_8e8k_jiWcxvDoabn0Ph_8O1h-vL0pR_wI1Zrqe</recordid><startdate>200212</startdate><enddate>200212</enddate><creator>Crivellato, Enrico</creator><creator>Candussio, Luigi</creator><creator>Mallardi, Franco</creator><creator>Ribatti, Domenico</creator><general>Blackwell Science Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200212</creationdate><title>Recombinant human alpha‐2a interferon promotes an atypical process of mast cell secretion with ultrastructural features suggestive for piecemeal degranulation</title><author>Crivellato, Enrico ; Candussio, Luigi ; Mallardi, Franco ; Ribatti, Domenico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4716-276c6a39b9e652295967a5571334ad064fe8ea6b9837ab37a7250bf443da4ff23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>atypical degranulation</topic><topic>Brief Communications</topic><topic>Cell Degranulation</topic><topic>Cells, Cultured</topic><topic>Histamine Release</topic><topic>Humans</topic><topic>interferon</topic><topic>Interferon alpha-2</topic><topic>Interferon-alpha - pharmacology</topic><topic>mast cell</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - physiology</topic><topic>Mast Cells - ultrastructure</topic><topic>Microscopy, Electron</topic><topic>Peritoneum</topic><topic>piecemeal degranulation</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recombinant Proteins</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crivellato, Enrico</creatorcontrib><creatorcontrib>Candussio, Luigi</creatorcontrib><creatorcontrib>Mallardi, Franco</creatorcontrib><creatorcontrib>Ribatti, Domenico</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of anatomy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crivellato, Enrico</au><au>Candussio, Luigi</au><au>Mallardi, Franco</au><au>Ribatti, Domenico</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant human alpha‐2a interferon promotes an atypical process of mast cell secretion with ultrastructural features suggestive for piecemeal degranulation</atitle><jtitle>Journal of anatomy</jtitle><addtitle>J Anat</addtitle><date>2002-12</date><risdate>2002</risdate><volume>201</volume><issue>6</issue><spage>507</spage><epage>512</epage><pages>507-512</pages><issn>0021-8782</issn><eissn>1469-7580</eissn><abstract>Purified mast cells (MC), isolated from the rat peritoneum, were stimulated in vitro with recombinant human IFN‐α 2a (rhIFN‐α 2a) and studied ultrastructurally. Quantitative determination of histamine release was also performed. The following ultrastructural features were observed: (1) dilation of single granules, leading to the formation of cytoplasmic cavities filled with dissolved or eroded matrices; (2) induction of partially empty, non‐fused granule containers close to unaltered resting granules, a process very suggestive of piecemeal degranulation; (3) focal exocytosis, characterized by opening of single granules to the cell exterior and/or fusion of a few granules into small secretory channels. Histamine release was slightly increased in rhIFN‐α 2a‐treated MC, although not to significant levels. These results indicate that rhIFN‐α 2a induces a characteristic pattern of degranulation in rat peritoneal MC and that a small proportion of rhIFN‐α 2a‐stimulated MC shows ultrastructural features suggestive for piecemeal degranulation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12489762</pmid><doi>10.1046/j.1469-7580.2002.00116.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals atypical degranulation Brief Communications Cell Degranulation Cells, Cultured Histamine Release Humans interferon Interferon alpha-2 Interferon-alpha - pharmacology mast cell Mast Cells - drug effects Mast Cells - physiology Mast Cells - ultrastructure Microscopy, Electron Peritoneum piecemeal degranulation rat Rats Rats, Wistar Recombinant Proteins Stimulation, Chemical |
| title | Recombinant human alpha‐2a interferon promotes an atypical process of mast cell secretion with ultrastructural features suggestive for piecemeal degranulation |
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