Bioassay of Genotoxic Effects of Environmental Particles in a Feeding Ciliate
The ciliated protozoan, Paramecium, can be used to quantitate cytotoxic and genotoxic effects of ingested complex environmental particles. Cytotoxicity is quantitated by the increased proportion of treated versus control cells which do not retain their capacity for normal cell replication. Genotoxic...
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| Veröffentlicht in: | Environmental health perspectives 1983-09, Vol.51, p.205-210 |
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| creator | Smith-Sonneborn, Joan Palizzi, Ronald A. McCann, Elizabeth A. Fisher, Gerald L. |
| description | The ciliated protozoan, Paramecium, can be used to quantitate cytotoxic and genotoxic effects of ingested complex environmental particles. Cytotoxicity is quantitated by the increased proportion of treated versus control cells which do not retain their capacity for normal cell replication. Genotoxic effects are assessed by the increased fraction of nonviable offspring from treated versus control parent cells after the self-fertilization process of autogamy. Since these cells ingest nonnutrient respirable-sized particles, biological activity of intracellular extraction of dusts and fly ash can be compared before and after extraction with polar and nonpolar solvents. Previous studies indicated that coal fly ash was mutagenic in these eukaryotic cells. Mutagenicity of coal fly ash was not detectable after extraction with a concentration of HCl known to remove nonmatrix trace elements. These results suggested that this ciliate bioassay might be a detector of mineral mutagens. Fine particles of the carcinogenic nickel compounds, α-nickel subsulfide, and β-nickel sulfide were compared for their biological activity in this bioassay. Both nickel compounds were ingested by the ciliates and induced heritable damage in the progeny of the treated parent cells. |
| doi_str_mv | 10.1289/ehp.8351205 |
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Cytotoxicity is quantitated by the increased proportion of treated versus control cells which do not retain their capacity for normal cell replication. Genotoxic effects are assessed by the increased fraction of nonviable offspring from treated versus control parent cells after the self-fertilization process of autogamy. Since these cells ingest nonnutrient respirable-sized particles, biological activity of intracellular extraction of dusts and fly ash can be compared before and after extraction with polar and nonpolar solvents. Previous studies indicated that coal fly ash was mutagenic in these eukaryotic cells. Mutagenicity of coal fly ash was not detectable after extraction with a concentration of HCl known to remove nonmatrix trace elements. These results suggested that this ciliate bioassay might be a detector of mineral mutagens. Fine particles of the carcinogenic nickel compounds, α-nickel subsulfide, and β-nickel sulfide were compared for their biological activity in this bioassay. Both nickel compounds were ingested by the ciliates and induced heritable damage in the progeny of the treated parent cells.</description><identifier>ISSN: 0091-6765</identifier><identifier>EISSN: 1552-9924</identifier><identifier>DOI: 10.1289/ehp.8351205</identifier><identifier>PMID: 6641654</identifier><language>eng</language><publisher>United States: National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare</publisher><subject>Animals ; Autogamy ; Bioassay ; Cell Survival - drug effects ; Coal fly ash ; Cytotoxicity ; DNA damage ; Dust - adverse effects ; Environmental Pollutants - toxicity ; Mutagenicity ; Mutagenicity Tests ; Mutagens ; Nickel ; Nickel - toxicity ; Nickel compounds ; Paramecium ; Paramecium - genetics ; Self fertilization ; Sulfides ; The Second International Workshop on in Vitro Effects of Mineral Dusts. April 25-28, 1982. Arkadelphia, Arkansas</subject><ispartof>Environmental health perspectives, 1983-09, Vol.51, p.205-210</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-7419a493bd61342ee60d34689780c45a9df1afe92ccbd9e3147b1a35f2502133</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3429750$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3429750$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,309,310,314,723,776,780,785,786,799,860,881,23909,23910,25118,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6641654$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith-Sonneborn, Joan</creatorcontrib><creatorcontrib>Palizzi, Ronald A.</creatorcontrib><creatorcontrib>McCann, Elizabeth A.</creatorcontrib><creatorcontrib>Fisher, Gerald L.</creatorcontrib><title>Bioassay of Genotoxic Effects of Environmental Particles in a Feeding Ciliate</title><title>Environmental health perspectives</title><addtitle>Environ Health Perspect</addtitle><description>The ciliated protozoan, Paramecium, can be used to quantitate cytotoxic and genotoxic effects of ingested complex environmental particles. Cytotoxicity is quantitated by the increased proportion of treated versus control cells which do not retain their capacity for normal cell replication. Genotoxic effects are assessed by the increased fraction of nonviable offspring from treated versus control parent cells after the self-fertilization process of autogamy. Since these cells ingest nonnutrient respirable-sized particles, biological activity of intracellular extraction of dusts and fly ash can be compared before and after extraction with polar and nonpolar solvents. Previous studies indicated that coal fly ash was mutagenic in these eukaryotic cells. Mutagenicity of coal fly ash was not detectable after extraction with a concentration of HCl known to remove nonmatrix trace elements. These results suggested that this ciliate bioassay might be a detector of mineral mutagens. Fine particles of the carcinogenic nickel compounds, α-nickel subsulfide, and β-nickel sulfide were compared for their biological activity in this bioassay. Both nickel compounds were ingested by the ciliates and induced heritable damage in the progeny of the treated parent cells.</description><subject>Animals</subject><subject>Autogamy</subject><subject>Bioassay</subject><subject>Cell Survival - drug effects</subject><subject>Coal fly ash</subject><subject>Cytotoxicity</subject><subject>DNA damage</subject><subject>Dust - adverse effects</subject><subject>Environmental Pollutants - toxicity</subject><subject>Mutagenicity</subject><subject>Mutagenicity Tests</subject><subject>Mutagens</subject><subject>Nickel</subject><subject>Nickel - toxicity</subject><subject>Nickel compounds</subject><subject>Paramecium</subject><subject>Paramecium - genetics</subject><subject>Self fertilization</subject><subject>Sulfides</subject><subject>The Second International Workshop on in Vitro Effects of Mineral Dusts. April 25-28, 1982. Arkadelphia, Arkansas</subject><issn>0091-6765</issn><issn>1552-9924</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1PAjEQxRujQfw4eTbZg_FiFvu924uJEkATjB64N6U7CyXLFtuFyH_vEgjB0yTz3rx5-SF0R3CP0Fw9w3zVy5kgFIsz1CVC0FQpys9RF2NFUplJcYmuYlxgjEkuZQd1pORECt5Fn2_OmxjNNvFlMoLaN_7X2WRQlmCbuFsO6o0Lvl5C3Zgq-TahcbaCmLg6MckQoHD1LOm7ypkGbtBFaaoIt4d5jSbDwaT_no6_Rh_913FqBaZNmnGiDFdsWkjCOAWQuGBc5irLseXCqKIkpgRFrZ0WChjh2ZQYJkranhPGrtHLPna1ni6hsG21YCq9Cm5pwlZ74_R_pXZzPfMbTYRUNONtwOMhIPifNcRGL120UFWmBr-OmrCMUsFwa3zaG23wMQYoj08I1jv4uoWvD_Bb9_1pr6P3QLvVH_b6IjY-nEZRhjPdolCZwOwP5YiLkw</recordid><startdate>19830901</startdate><enddate>19830901</enddate><creator>Smith-Sonneborn, Joan</creator><creator>Palizzi, Ronald A.</creator><creator>McCann, Elizabeth A.</creator><creator>Fisher, Gerald L.</creator><general>National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7TV</scope><scope>7U2</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19830901</creationdate><title>Bioassay of Genotoxic Effects of Environmental Particles in a Feeding Ciliate</title><author>Smith-Sonneborn, Joan ; Palizzi, Ronald A. ; McCann, Elizabeth A. ; Fisher, Gerald L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-7419a493bd61342ee60d34689780c45a9df1afe92ccbd9e3147b1a35f2502133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Autogamy</topic><topic>Bioassay</topic><topic>Cell Survival - drug effects</topic><topic>Coal fly ash</topic><topic>Cytotoxicity</topic><topic>DNA damage</topic><topic>Dust - adverse effects</topic><topic>Environmental Pollutants - toxicity</topic><topic>Mutagenicity</topic><topic>Mutagenicity Tests</topic><topic>Mutagens</topic><topic>Nickel</topic><topic>Nickel - toxicity</topic><topic>Nickel compounds</topic><topic>Paramecium</topic><topic>Paramecium - genetics</topic><topic>Self fertilization</topic><topic>Sulfides</topic><topic>The Second International Workshop on in Vitro Effects of Mineral Dusts. April 25-28, 1982. Arkadelphia, Arkansas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith-Sonneborn, Joan</creatorcontrib><creatorcontrib>Palizzi, Ronald A.</creatorcontrib><creatorcontrib>McCann, Elizabeth A.</creatorcontrib><creatorcontrib>Fisher, Gerald L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Pollution Abstracts</collection><collection>Safety Science and Risk</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Environmental health perspectives</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith-Sonneborn, Joan</au><au>Palizzi, Ronald A.</au><au>McCann, Elizabeth A.</au><au>Fisher, Gerald L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioassay of Genotoxic Effects of Environmental Particles in a Feeding Ciliate</atitle><jtitle>Environmental health perspectives</jtitle><addtitle>Environ Health Perspect</addtitle><date>1983-09-01</date><risdate>1983</risdate><volume>51</volume><spage>205</spage><epage>210</epage><pages>205-210</pages><issn>0091-6765</issn><eissn>1552-9924</eissn><abstract>The ciliated protozoan, Paramecium, can be used to quantitate cytotoxic and genotoxic effects of ingested complex environmental particles. Cytotoxicity is quantitated by the increased proportion of treated versus control cells which do not retain their capacity for normal cell replication. Genotoxic effects are assessed by the increased fraction of nonviable offspring from treated versus control parent cells after the self-fertilization process of autogamy. Since these cells ingest nonnutrient respirable-sized particles, biological activity of intracellular extraction of dusts and fly ash can be compared before and after extraction with polar and nonpolar solvents. Previous studies indicated that coal fly ash was mutagenic in these eukaryotic cells. Mutagenicity of coal fly ash was not detectable after extraction with a concentration of HCl known to remove nonmatrix trace elements. These results suggested that this ciliate bioassay might be a detector of mineral mutagens. Fine particles of the carcinogenic nickel compounds, α-nickel subsulfide, and β-nickel sulfide were compared for their biological activity in this bioassay. Both nickel compounds were ingested by the ciliates and induced heritable damage in the progeny of the treated parent cells.</abstract><cop>United States</cop><pub>National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare</pub><pmid>6641654</pmid><doi>10.1289/ehp.8351205</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Animals Autogamy Bioassay Cell Survival - drug effects Coal fly ash Cytotoxicity DNA damage Dust - adverse effects Environmental Pollutants - toxicity Mutagenicity Mutagenicity Tests Mutagens Nickel Nickel - toxicity Nickel compounds Paramecium Paramecium - genetics Self fertilization Sulfides The Second International Workshop on in Vitro Effects of Mineral Dusts. April 25-28, 1982. Arkadelphia, Arkansas |
| title | Bioassay of Genotoxic Effects of Environmental Particles in a Feeding Ciliate |
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