Augmented acetylcholine‐induced, Rho‐mediated Ca2+ sensitization of bronchial smooth muscle contraction in antigen‐induced airway hyperresponsive rats
Treatment with acetylcholine (ACh) of a β‐escin‐permeabilized intrapulmonary bronchial smooth muscle of the rat induced force when the Ca2+ concentration was clamped at 1 μM. The ACh‐induced Ca2+ sensitization of myofilaments was significantly greater in antigen‐induced airway hyperresponsive rats t...
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| Veröffentlicht in: | British journal of pharmacology 1999-06, Vol.127 (3), p.597-600 |
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| container_title | British journal of pharmacology |
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| creator | Chiba, Yoshihiko Takada, Yuka Miyamoto, Shigeki Mitsui‐Saito, Minori Karaki, Hideaki Misawa, Miwa |
| description | Treatment with acetylcholine (ACh) of a β‐escin‐permeabilized intrapulmonary bronchial smooth muscle of the rat induced force when the Ca2+ concentration was clamped at 1 μM. The ACh‐induced Ca2+ sensitization of myofilaments was significantly greater in antigen‐induced airway hyperresponsive rats than in control rats. The ACh‐induced Ca2+ sensitization was completely blocked by treatment with Clostridium botulinum C3 exoenzyme, an inactivator of Rho family of proteins. Moreover, the protein level of RhoA in the intrapulmonary bronchi was significantly increased in the airway hyperresponsive rats. Thus, increased airway smooth muscle contractility observed in asthmatics may be related to augmented agonist‐induced, Rho‐mediated Ca2+ sensitization of myofilaments.
British Journal of Pharmacology (1999) 127, 597–600; doi:10.1038/sj.bjp.0702585 |
| doi_str_mv | 10.1038/sj.bjp.0702585 |
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British Journal of Pharmacology (1999) 127, 597–600; doi:10.1038/sj.bjp.0702585</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1038/sj.bjp.0702585</identifier><identifier>PMID: 10401547</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetylcholine - pharmacology ; Actin Cytoskeleton - physiology ; airway hyperresponsiveness ; Animals ; Antigens - immunology ; Asthma ; Biological and medical sciences ; Bronchi - drug effects ; Bronchi - metabolism ; Bronchi - physiopathology ; Bronchial Hyperreactivity - physiopathology ; Ca2+ sensitization ; Calcium - pharmacology ; Calcium - physiology ; Escin - pharmacology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; GTP-Binding Proteins - physiology ; Immediate hypersensitivity. Allergy. Anaphylaxis, etc ; Immunobiology ; In Vitro Techniques ; intrapulmonary bronchial smooth muscle ; Male ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Muscle, Smooth - physiopathology ; Rats ; Rats, Wistar ; Reaction mechanisms, antibodies, chemical mediators ; Rho ; Special Reports ; β‐escin</subject><ispartof>British journal of pharmacology, 1999-06, Vol.127 (3), p.597-600</ispartof><rights>1999 Nature Publishing Group</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999, Nature Publishing Group 1999 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566055/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566055/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1416,1432,27922,27923,45572,45573,46407,46831,53789,53791</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1834284$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10401547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiba, Yoshihiko</creatorcontrib><creatorcontrib>Takada, Yuka</creatorcontrib><creatorcontrib>Miyamoto, Shigeki</creatorcontrib><creatorcontrib>Mitsui‐Saito, Minori</creatorcontrib><creatorcontrib>Karaki, Hideaki</creatorcontrib><creatorcontrib>Misawa, Miwa</creatorcontrib><title>Augmented acetylcholine‐induced, Rho‐mediated Ca2+ sensitization of bronchial smooth muscle contraction in antigen‐induced airway hyperresponsive rats</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>Treatment with acetylcholine (ACh) of a β‐escin‐permeabilized intrapulmonary bronchial smooth muscle of the rat induced force when the Ca2+ concentration was clamped at 1 μM. The ACh‐induced Ca2+ sensitization of myofilaments was significantly greater in antigen‐induced airway hyperresponsive rats than in control rats. The ACh‐induced Ca2+ sensitization was completely blocked by treatment with Clostridium botulinum C3 exoenzyme, an inactivator of Rho family of proteins. Moreover, the protein level of RhoA in the intrapulmonary bronchi was significantly increased in the airway hyperresponsive rats. Thus, increased airway smooth muscle contractility observed in asthmatics may be related to augmented agonist‐induced, Rho‐mediated Ca2+ sensitization of myofilaments.
British Journal of Pharmacology (1999) 127, 597–600; doi:10.1038/sj.bjp.0702585</description><subject>Acetylcholine - pharmacology</subject><subject>Actin Cytoskeleton - physiology</subject><subject>airway hyperresponsiveness</subject><subject>Animals</subject><subject>Antigens - immunology</subject><subject>Asthma</subject><subject>Biological and medical sciences</subject><subject>Bronchi - drug effects</subject><subject>Bronchi - metabolism</subject><subject>Bronchi - physiopathology</subject><subject>Bronchial Hyperreactivity - physiopathology</subject><subject>Ca2+ sensitization</subject><subject>Calcium - pharmacology</subject><subject>Calcium - physiology</subject><subject>Escin - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>GTP-Binding Proteins - physiology</subject><subject>Immediate hypersensitivity. Allergy. Anaphylaxis, etc</subject><subject>Immunobiology</subject><subject>In Vitro Techniques</subject><subject>intrapulmonary bronchial smooth muscle</subject><subject>Male</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - physiopathology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reaction mechanisms, antibodies, chemical mediators</subject><subject>Rho</subject><subject>Special Reports</subject><subject>β‐escin</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVksFu1DAQhiMEokvhyhH5gLhAFju2E-eCVFZAkSqBEJytWcfeOHLsxU5ahROPwAPwdDwJXrrQchqN5tM3Y_kviscErwmm4mUa1tthv8YNrrjgd4oVYU1dcirI3WKFMW5KQoQ4KR6kNGCchw2_X5wQzDDhrFkVP8_m3aj9pDsESk-LU31w1utf339Y381Kdy_Qpz7kdtSdhQO3geo5StonO9lvMNngUTBoG4NXvQWH0hjC1KNxTspppIKfIqg_mPUI_GR32t_oEdh4BQvql72OUad9yOJLjSJM6WFxz4BL-tGxnhZf3r75vDkvLz68e785uygH2lBWttCAaHndEUNJXRkNTY073LaEYVVh1rVbYrpcalJxY2rNDRGAQXFmKBhDT4tX1979vM3PVPpwspP7aEeIiwxg5f8Tb3u5C5eS8LrGnGfBs6Mghq-zTpMcbVLaOfA6zEnWrRAYVzSDT25v-rfi74dk4OkRgKTAmQhe2XTDCcoqwTJGr7Er6_RySyMPqZBpkDkV8pgK-frjOSEto78BVbKzQA</recordid><startdate>199906</startdate><enddate>199906</enddate><creator>Chiba, Yoshihiko</creator><creator>Takada, Yuka</creator><creator>Miyamoto, Shigeki</creator><creator>Mitsui‐Saito, Minori</creator><creator>Karaki, Hideaki</creator><creator>Misawa, Miwa</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199906</creationdate><title>Augmented acetylcholine‐induced, Rho‐mediated Ca2+ sensitization of bronchial smooth muscle contraction in antigen‐induced airway hyperresponsive rats</title><author>Chiba, Yoshihiko ; Takada, Yuka ; Miyamoto, Shigeki ; Mitsui‐Saito, Minori ; Karaki, Hideaki ; Misawa, Miwa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3734-9a7a8956d1f3162fea760d099140c204d9b1fd4d96125ff6e5f18a0ac54f3aff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acetylcholine - pharmacology</topic><topic>Actin Cytoskeleton - physiology</topic><topic>airway hyperresponsiveness</topic><topic>Animals</topic><topic>Antigens - immunology</topic><topic>Asthma</topic><topic>Biological and medical sciences</topic><topic>Bronchi - drug effects</topic><topic>Bronchi - metabolism</topic><topic>Bronchi - physiopathology</topic><topic>Bronchial Hyperreactivity - physiopathology</topic><topic>Ca2+ sensitization</topic><topic>Calcium - pharmacology</topic><topic>Calcium - physiology</topic><topic>Escin - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>GTP-Binding Proteins - physiology</topic><topic>Immediate hypersensitivity. Allergy. Anaphylaxis, etc</topic><topic>Immunobiology</topic><topic>In Vitro Techniques</topic><topic>intrapulmonary bronchial smooth muscle</topic><topic>Male</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - physiopathology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reaction mechanisms, antibodies, chemical mediators</topic><topic>Rho</topic><topic>Special Reports</topic><topic>β‐escin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiba, Yoshihiko</creatorcontrib><creatorcontrib>Takada, Yuka</creatorcontrib><creatorcontrib>Miyamoto, Shigeki</creatorcontrib><creatorcontrib>Mitsui‐Saito, Minori</creatorcontrib><creatorcontrib>Karaki, Hideaki</creatorcontrib><creatorcontrib>Misawa, Miwa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiba, Yoshihiko</au><au>Takada, Yuka</au><au>Miyamoto, Shigeki</au><au>Mitsui‐Saito, Minori</au><au>Karaki, Hideaki</au><au>Misawa, Miwa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Augmented acetylcholine‐induced, Rho‐mediated Ca2+ sensitization of bronchial smooth muscle contraction in antigen‐induced airway hyperresponsive rats</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>1999-06</date><risdate>1999</risdate><volume>127</volume><issue>3</issue><spage>597</spage><epage>600</epage><pages>597-600</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>Treatment with acetylcholine (ACh) of a β‐escin‐permeabilized intrapulmonary bronchial smooth muscle of the rat induced force when the Ca2+ concentration was clamped at 1 μM. The ACh‐induced Ca2+ sensitization of myofilaments was significantly greater in antigen‐induced airway hyperresponsive rats than in control rats. The ACh‐induced Ca2+ sensitization was completely blocked by treatment with Clostridium botulinum C3 exoenzyme, an inactivator of Rho family of proteins. Moreover, the protein level of RhoA in the intrapulmonary bronchi was significantly increased in the airway hyperresponsive rats. Thus, increased airway smooth muscle contractility observed in asthmatics may be related to augmented agonist‐induced, Rho‐mediated Ca2+ sensitization of myofilaments.
British Journal of Pharmacology (1999) 127, 597–600; doi:10.1038/sj.bjp.0702585</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10401547</pmid><doi>10.1038/sj.bjp.0702585</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Wiley Online Library All Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acetylcholine - pharmacology Actin Cytoskeleton - physiology airway hyperresponsiveness Animals Antigens - immunology Asthma Biological and medical sciences Bronchi - drug effects Bronchi - metabolism Bronchi - physiopathology Bronchial Hyperreactivity - physiopathology Ca2+ sensitization Calcium - pharmacology Calcium - physiology Escin - pharmacology Fundamental and applied biological sciences. Psychology Fundamental immunology GTP-Binding Proteins - physiology Immediate hypersensitivity. Allergy. Anaphylaxis, etc Immunobiology In Vitro Techniques intrapulmonary bronchial smooth muscle Male Muscle Contraction - drug effects Muscle, Smooth - drug effects Muscle, Smooth - physiopathology Rats Rats, Wistar Reaction mechanisms, antibodies, chemical mediators Rho Special Reports β‐escin |
| title | Augmented acetylcholine‐induced, Rho‐mediated Ca2+ sensitization of bronchial smooth muscle contraction in antigen‐induced airway hyperresponsive rats |
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