Effects of dexamethasone on airway hyper‐responsiveness to the adenosine A1 receptor agonist cyclo‐pentyl adenosine in an allergic rabbit model
New Zealand White (NZW) rabbits were immunized within 24 h of birth with Alternaria tenuis in aluminium hydroxide (Al (OH)3) (i.p.) or sham immunized (saline plus Al (OH)3 i.p.) and subsequently injected with the allergen (i.p.) or sham‐immunized for the next 3 months. At 3 months of age, baseline a...
Gespeichert in:
| Veröffentlicht in: | British journal of pharmacology 1999-03, Vol.126 (6), p.1513-1521 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1521 |
|---|---|
| container_issue | 6 |
| container_start_page | 1513 |
| container_title | British journal of pharmacology |
| container_volume | 126 |
| creator | El‐Hashim, Ahmed Z Banner, Katharine H Paul, William Page, Clive P |
| description | New Zealand White (NZW) rabbits were immunized within 24 h of birth with Alternaria tenuis in aluminium hydroxide (Al (OH)3) (i.p.) or sham immunized (saline plus Al (OH)3 i.p.) and subsequently injected with the allergen (i.p.) or sham‐immunized for the next 3 months. At 3 months of age, baseline airway responsiveness was assessed using cyclo‐pentyl adenosine (CPA). Bronchoalveolar lavage (BAL) was performed in all animals and samples of peripheral blood were collected from some animals for estimation of dexamethasone levels. In some animals, blood was collected at the end of the experiment and cellular function was assessed by measurement of ex vivo proliferation of mononuclear cells in response to phytohaemagglutinin (PHA).
Allergen immunization significantly increased baseline airway responsiveness to inhaled CPA (P |
| doi_str_mv | 10.1038/sj.bjp.0702455 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1565923</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>BPH1069</sourcerecordid><originalsourceid>FETCH-LOGICAL-p2909-7a960abcd62eef18db0964dc33ae262f3fb3be222b524e074ead3ebb5600376c3</originalsourceid><addsrcrecordid>eNpVkU9rFDEYh4NY7Fq9epQcvM6aP5Nk5iLUUq1QqAc9hyTzzm6WbDIkY-vc_AiC39BPYqRru8ILObzP7-ElP4ReUbKmhHdvy25td9OaKMJaIZ6gFW2VbATv6FO0IoSohtKuO0XPS9kRUpdKPEOnlDCqRKtW6NflOIKbC04jHuC72cO8NSVFwCli4_OdWfB2mSD__vEzQ5lSLP4WIpSC54TnLWAzQEzF18Q5xRkcTHPK2GxS9GXGbnEh1ewEcV7CEeyrvk4IkDfe4Wys9TPepwHCC3QymlDg5eE9Q18_XH65uGqubz5-uji_bibWk75RppfEWDdIBjDSbrCkl-3gODfAJBv5aLkFxpgVrAWiWjADB2uFJIQr6fgZenfvnb7ZPQyunphN0FP2e5MXnYzX_2-i3-pNutVUSNEzXgWvjwUPyX_fW4E3B8AUZ8KYTXS-PHKqcm1XMX6P3fkAy5FG_-1Yl52uHetDx_r95ytKZM__AAi2oV4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effects of dexamethasone on airway hyper‐responsiveness to the adenosine A1 receptor agonist cyclo‐pentyl adenosine in an allergic rabbit model</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>El‐Hashim, Ahmed Z ; Banner, Katharine H ; Paul, William ; Page, Clive P</creator><creatorcontrib>El‐Hashim, Ahmed Z ; Banner, Katharine H ; Paul, William ; Page, Clive P</creatorcontrib><description>New Zealand White (NZW) rabbits were immunized within 24 h of birth with Alternaria tenuis in aluminium hydroxide (Al (OH)3) (i.p.) or sham immunized (saline plus Al (OH)3 i.p.) and subsequently injected with the allergen (i.p.) or sham‐immunized for the next 3 months. At 3 months of age, baseline airway responsiveness was assessed using cyclo‐pentyl adenosine (CPA). Bronchoalveolar lavage (BAL) was performed in all animals and samples of peripheral blood were collected from some animals for estimation of dexamethasone levels. In some animals, blood was collected at the end of the experiment and cellular function was assessed by measurement of ex vivo proliferation of mononuclear cells in response to phytohaemagglutinin (PHA).
Allergen immunization significantly increased baseline airway responsiveness to inhaled CPA (P<0.05) in comparison with sham‐immunized animals, at 3 months after immunization. Dexamethasone (0.5 mg kg−1 day−1) treatment for 1 month did not modify this established airway hyper‐responsiveness to CPA. Dexamethasone treatment did not affect either total or differential cell numbers in BAL fluid during the 4 week period, although significant plasma levels of dexamethasone were achieved in dexamethasone treated animals.
Treatment of rabbits with dexamethasone (0.1 mg kg−1 i.p.), 6 h prior to each allergen injection from the neonatal stage, significantly reduced baseline airway hyper‐responsiveness to CPA measured at 3 months (P<0.05). There was no significant difference in either total or differential cell numbers in BAL fluid, or any difference in mitogen‐induced proliferation of mononuclear cells between dexamethasone and vehicle treated rabbits.
These results suggest that introduction of glucocorticosteroids in early life can prevent baseline airway hyper‐responsiveness to inhaled CPA in allergic rabbits. However, once established, such underlying airway hyper‐responsiveness is difficult to resolve, even with prolonged treatment with glucocorticosteroids.
British Journal of Pharmacology (1999) 126, 1513–1521; doi:10.1038/sj.bjp.0702455</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1038/sj.bjp.0702455</identifier><identifier>PMID: 10217547</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenosine - analogs & derivatives ; Adenosine - pharmacology ; adenosine A1 receptor ; allergic inflammation ; Animals ; Baseline airway hyper‐responsiveness ; Biological and medical sciences ; Bronchial Hyperreactivity - chemically induced ; Bronchial Hyperreactivity - prevention & control ; Bronchoalveolar Lavage Fluid - cytology ; Cell Count - drug effects ; Cell Division - drug effects ; Dexamethasone - blood ; Dexamethasone - pharmacology ; Disease Models, Animal ; Female ; Glucocorticoids - pharmacology ; glucocorticosteroid ; Hypersensitivity - prevention & control ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - drug effects ; Male ; Medical sciences ; Pharmacology. Drug treatments ; Phytohemagglutinins - pharmacology ; Purinergic P1 Receptor Agonists ; Rabbits ; Respiratory system</subject><ispartof>British journal of pharmacology, 1999-03, Vol.126 (6), p.1513-1521</ispartof><rights>1999 Nature Publishing Group</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999, Nature Publishing Group 1999 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1565923/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1565923/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1416,1432,27923,27924,45573,45574,46408,46832,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1721748$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10217547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El‐Hashim, Ahmed Z</creatorcontrib><creatorcontrib>Banner, Katharine H</creatorcontrib><creatorcontrib>Paul, William</creatorcontrib><creatorcontrib>Page, Clive P</creatorcontrib><title>Effects of dexamethasone on airway hyper‐responsiveness to the adenosine A1 receptor agonist cyclo‐pentyl adenosine in an allergic rabbit model</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>New Zealand White (NZW) rabbits were immunized within 24 h of birth with Alternaria tenuis in aluminium hydroxide (Al (OH)3) (i.p.) or sham immunized (saline plus Al (OH)3 i.p.) and subsequently injected with the allergen (i.p.) or sham‐immunized for the next 3 months. At 3 months of age, baseline airway responsiveness was assessed using cyclo‐pentyl adenosine (CPA). Bronchoalveolar lavage (BAL) was performed in all animals and samples of peripheral blood were collected from some animals for estimation of dexamethasone levels. In some animals, blood was collected at the end of the experiment and cellular function was assessed by measurement of ex vivo proliferation of mononuclear cells in response to phytohaemagglutinin (PHA).
Allergen immunization significantly increased baseline airway responsiveness to inhaled CPA (P<0.05) in comparison with sham‐immunized animals, at 3 months after immunization. Dexamethasone (0.5 mg kg−1 day−1) treatment for 1 month did not modify this established airway hyper‐responsiveness to CPA. Dexamethasone treatment did not affect either total or differential cell numbers in BAL fluid during the 4 week period, although significant plasma levels of dexamethasone were achieved in dexamethasone treated animals.
Treatment of rabbits with dexamethasone (0.1 mg kg−1 i.p.), 6 h prior to each allergen injection from the neonatal stage, significantly reduced baseline airway hyper‐responsiveness to CPA measured at 3 months (P<0.05). There was no significant difference in either total or differential cell numbers in BAL fluid, or any difference in mitogen‐induced proliferation of mononuclear cells between dexamethasone and vehicle treated rabbits.
These results suggest that introduction of glucocorticosteroids in early life can prevent baseline airway hyper‐responsiveness to inhaled CPA in allergic rabbits. However, once established, such underlying airway hyper‐responsiveness is difficult to resolve, even with prolonged treatment with glucocorticosteroids.
British Journal of Pharmacology (1999) 126, 1513–1521; doi:10.1038/sj.bjp.0702455</description><subject>Adenosine - analogs & derivatives</subject><subject>Adenosine - pharmacology</subject><subject>adenosine A1 receptor</subject><subject>allergic inflammation</subject><subject>Animals</subject><subject>Baseline airway hyper‐responsiveness</subject><subject>Biological and medical sciences</subject><subject>Bronchial Hyperreactivity - chemically induced</subject><subject>Bronchial Hyperreactivity - prevention & control</subject><subject>Bronchoalveolar Lavage Fluid - cytology</subject><subject>Cell Count - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Dexamethasone - blood</subject><subject>Dexamethasone - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Glucocorticoids - pharmacology</subject><subject>glucocorticosteroid</subject><subject>Hypersensitivity - prevention & control</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Purinergic P1 Receptor Agonists</subject><subject>Rabbits</subject><subject>Respiratory system</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9rFDEYh4NY7Fq9epQcvM6aP5Nk5iLUUq1QqAc9hyTzzm6WbDIkY-vc_AiC39BPYqRru8ILObzP7-ElP4ReUbKmhHdvy25td9OaKMJaIZ6gFW2VbATv6FO0IoSohtKuO0XPS9kRUpdKPEOnlDCqRKtW6NflOIKbC04jHuC72cO8NSVFwCli4_OdWfB2mSD__vEzQ5lSLP4WIpSC54TnLWAzQEzF18Q5xRkcTHPK2GxS9GXGbnEh1ewEcV7CEeyrvk4IkDfe4Wys9TPepwHCC3QymlDg5eE9Q18_XH65uGqubz5-uji_bibWk75RppfEWDdIBjDSbrCkl-3gODfAJBv5aLkFxpgVrAWiWjADB2uFJIQr6fgZenfvnb7ZPQyunphN0FP2e5MXnYzX_2-i3-pNutVUSNEzXgWvjwUPyX_fW4E3B8AUZ8KYTXS-PHKqcm1XMX6P3fkAy5FG_-1Yl52uHetDx_r95ytKZM__AAi2oV4</recordid><startdate>199903</startdate><enddate>199903</enddate><creator>El‐Hashim, Ahmed Z</creator><creator>Banner, Katharine H</creator><creator>Paul, William</creator><creator>Page, Clive P</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>199903</creationdate><title>Effects of dexamethasone on airway hyper‐responsiveness to the adenosine A1 receptor agonist cyclo‐pentyl adenosine in an allergic rabbit model</title><author>El‐Hashim, Ahmed Z ; Banner, Katharine H ; Paul, William ; Page, Clive P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2909-7a960abcd62eef18db0964dc33ae262f3fb3be222b524e074ead3ebb5600376c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine - analogs & derivatives</topic><topic>Adenosine - pharmacology</topic><topic>adenosine A1 receptor</topic><topic>allergic inflammation</topic><topic>Animals</topic><topic>Baseline airway hyper‐responsiveness</topic><topic>Biological and medical sciences</topic><topic>Bronchial Hyperreactivity - chemically induced</topic><topic>Bronchial Hyperreactivity - prevention & control</topic><topic>Bronchoalveolar Lavage Fluid - cytology</topic><topic>Cell Count - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Dexamethasone - blood</topic><topic>Dexamethasone - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Glucocorticoids - pharmacology</topic><topic>glucocorticosteroid</topic><topic>Hypersensitivity - prevention & control</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Purinergic P1 Receptor Agonists</topic><topic>Rabbits</topic><topic>Respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El‐Hashim, Ahmed Z</creatorcontrib><creatorcontrib>Banner, Katharine H</creatorcontrib><creatorcontrib>Paul, William</creatorcontrib><creatorcontrib>Page, Clive P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El‐Hashim, Ahmed Z</au><au>Banner, Katharine H</au><au>Paul, William</au><au>Page, Clive P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of dexamethasone on airway hyper‐responsiveness to the adenosine A1 receptor agonist cyclo‐pentyl adenosine in an allergic rabbit model</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>1999-03</date><risdate>1999</risdate><volume>126</volume><issue>6</issue><spage>1513</spage><epage>1521</epage><pages>1513-1521</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>New Zealand White (NZW) rabbits were immunized within 24 h of birth with Alternaria tenuis in aluminium hydroxide (Al (OH)3) (i.p.) or sham immunized (saline plus Al (OH)3 i.p.) and subsequently injected with the allergen (i.p.) or sham‐immunized for the next 3 months. At 3 months of age, baseline airway responsiveness was assessed using cyclo‐pentyl adenosine (CPA). Bronchoalveolar lavage (BAL) was performed in all animals and samples of peripheral blood were collected from some animals for estimation of dexamethasone levels. In some animals, blood was collected at the end of the experiment and cellular function was assessed by measurement of ex vivo proliferation of mononuclear cells in response to phytohaemagglutinin (PHA).
Allergen immunization significantly increased baseline airway responsiveness to inhaled CPA (P<0.05) in comparison with sham‐immunized animals, at 3 months after immunization. Dexamethasone (0.5 mg kg−1 day−1) treatment for 1 month did not modify this established airway hyper‐responsiveness to CPA. Dexamethasone treatment did not affect either total or differential cell numbers in BAL fluid during the 4 week period, although significant plasma levels of dexamethasone were achieved in dexamethasone treated animals.
Treatment of rabbits with dexamethasone (0.1 mg kg−1 i.p.), 6 h prior to each allergen injection from the neonatal stage, significantly reduced baseline airway hyper‐responsiveness to CPA measured at 3 months (P<0.05). There was no significant difference in either total or differential cell numbers in BAL fluid, or any difference in mitogen‐induced proliferation of mononuclear cells between dexamethasone and vehicle treated rabbits.
These results suggest that introduction of glucocorticosteroids in early life can prevent baseline airway hyper‐responsiveness to inhaled CPA in allergic rabbits. However, once established, such underlying airway hyper‐responsiveness is difficult to resolve, even with prolonged treatment with glucocorticosteroids.
British Journal of Pharmacology (1999) 126, 1513–1521; doi:10.1038/sj.bjp.0702455</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10217547</pmid><doi>10.1038/sj.bjp.0702455</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1188 |
| ispartof | British journal of pharmacology, 1999-03, Vol.126 (6), p.1513-1521 |
| issn | 0007-1188 1476-5381 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1565923 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adenosine - analogs & derivatives Adenosine - pharmacology adenosine A1 receptor allergic inflammation Animals Baseline airway hyper‐responsiveness Biological and medical sciences Bronchial Hyperreactivity - chemically induced Bronchial Hyperreactivity - prevention & control Bronchoalveolar Lavage Fluid - cytology Cell Count - drug effects Cell Division - drug effects Dexamethasone - blood Dexamethasone - pharmacology Disease Models, Animal Female Glucocorticoids - pharmacology glucocorticosteroid Hypersensitivity - prevention & control Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Male Medical sciences Pharmacology. Drug treatments Phytohemagglutinins - pharmacology Purinergic P1 Receptor Agonists Rabbits Respiratory system |
| title | Effects of dexamethasone on airway hyper‐responsiveness to the adenosine A1 receptor agonist cyclo‐pentyl adenosine in an allergic rabbit model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T20%3A06%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20dexamethasone%20on%20airway%20hyper%E2%80%90responsiveness%20to%20the%20adenosine%20A1%20receptor%20agonist%20cyclo%E2%80%90pentyl%20adenosine%20in%20an%20allergic%20rabbit%20model&rft.jtitle=British%20journal%20of%20pharmacology&rft.au=El%E2%80%90Hashim,%20Ahmed%20Z&rft.date=1999-03&rft.volume=126&rft.issue=6&rft.spage=1513&rft.epage=1521&rft.pages=1513-1521&rft.issn=0007-1188&rft.eissn=1476-5381&rft.coden=BJPCBM&rft_id=info:doi/10.1038/sj.bjp.0702455&rft_dat=%3Cwiley_pubme%3EBPH1069%3C/wiley_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/10217547&rfr_iscdi=true |