Comparison of cannabinoid binding sites in guinea‐pig forebrain and small intestine
1 We have investigated the nature of cannabinoid receptors in guinea‐pig small intestine by establishing whether this tissue contains cannabinoid receptors with similar binding properties to those of brain CB1 receptors. The cannabinoids used were the CB1‐selective antagonist SR141716A, the CB2‐sele...
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| Veröffentlicht in: | British journal of pharmacology 1998-11, Vol.125 (6), p.1345-1351 |
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| creator | Ross, Ruth A Brockie, Heather C Fernando, Susanthi R Saha, Bijali Razdan, Raj K Pertwee, Roger G |
| description | 1
We have investigated the nature of cannabinoid receptors in guinea‐pig small intestine by establishing whether this tissue contains cannabinoid receptors with similar binding properties to those of brain CB1 receptors. The cannabinoids used were the CB1‐selective antagonist SR141716A, the CB2‐selective antagonist SR144528, the novel cannabinoid receptor ligand, 6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol (O‐1184), and the agonists CP55940, which binds equally well to CB1 and CB2 receptors, and WIN55212‐2, which shows marginal CB2 selectivity.
2
[3H]‐CP55940 (1 nm) underwent extensive specific binding both to forebrain membranes (76.3%) and to membranes obtained by sucrose density gradient fractionation of homogenates of myenteric plexus‐longitudinal muscle of guinea‐pig small intestine (65.2%).
3
Its binding capacity (Bmax) was higher in forebrain (4281 fmol mg−1) than in intestinal membranes (2092 fmol mg−1). However, the corresponding KD values were not significantly different from each other (2.29 and 1.75 nm respectively). Nor did the Ki values for its displacement by CP55940, WIN55212‐2, O‐1184, SR141716A and SR144528 from forebrain membranes (0.87, 4.15, 2.85, 5.32 and 371.9 respectively) differ significantly from the corresponding Ki values determined in experiments with intestinal membranes (0.99, 5.03, 3.16, 4.95 and 361.5 nm respectively).
4
The Bmax values of [3H]‐CP55940 and [3H]‐SR141716A in forebrain membranes did not differ significantly from each other (4281 and 5658 fmol mg−1) but were both greater than the Bmax of [3H]‐WIN55212‐2 (2032 fmol mg−1).
5
O‐1184 (10 or 100 nm) produced parallel dextral shifts in the log concentration‐response curves of WIN55212‐2 and CP55940 for inhibition of electrically‐evoked contractions of the myenteric plexus‐longitudinal muscle preparation, its KD values being 0.20 nm (against WIN55212‐2) and 0.89 nm (against CP55940).
6
We conclude that cannabinoid binding sites in guinea‐pig small intestine closely resemble CB1 binding sites of guinea‐pig brain and that O‐1184 behaves as a cannabinoid receptor antagonist in the guinea‐pig myenteric plexus‐longitudinal muscle preparation.
British Journal of Pharmacology (1998) 125, 1345–1351; doi:10.1038/sj.bjp.0702204 |
| doi_str_mv | 10.1038/sj.bjp.0702204 |
| format | Article |
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We have investigated the nature of cannabinoid receptors in guinea‐pig small intestine by establishing whether this tissue contains cannabinoid receptors with similar binding properties to those of brain CB1 receptors. The cannabinoids used were the CB1‐selective antagonist SR141716A, the CB2‐selective antagonist SR144528, the novel cannabinoid receptor ligand, 6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol (O‐1184), and the agonists CP55940, which binds equally well to CB1 and CB2 receptors, and WIN55212‐2, which shows marginal CB2 selectivity.
2
[3H]‐CP55940 (1 nm) underwent extensive specific binding both to forebrain membranes (76.3%) and to membranes obtained by sucrose density gradient fractionation of homogenates of myenteric plexus‐longitudinal muscle of guinea‐pig small intestine (65.2%).
3
Its binding capacity (Bmax) was higher in forebrain (4281 fmol mg−1) than in intestinal membranes (2092 fmol mg−1). However, the corresponding KD values were not significantly different from each other (2.29 and 1.75 nm respectively). Nor did the Ki values for its displacement by CP55940, WIN55212‐2, O‐1184, SR141716A and SR144528 from forebrain membranes (0.87, 4.15, 2.85, 5.32 and 371.9 respectively) differ significantly from the corresponding Ki values determined in experiments with intestinal membranes (0.99, 5.03, 3.16, 4.95 and 361.5 nm respectively).
4
The Bmax values of [3H]‐CP55940 and [3H]‐SR141716A in forebrain membranes did not differ significantly from each other (4281 and 5658 fmol mg−1) but were both greater than the Bmax of [3H]‐WIN55212‐2 (2032 fmol mg−1).
5
O‐1184 (10 or 100 nm) produced parallel dextral shifts in the log concentration‐response curves of WIN55212‐2 and CP55940 for inhibition of electrically‐evoked contractions of the myenteric plexus‐longitudinal muscle preparation, its KD values being 0.20 nm (against WIN55212‐2) and 0.89 nm (against CP55940).
6
We conclude that cannabinoid binding sites in guinea‐pig small intestine closely resemble CB1 binding sites of guinea‐pig brain and that O‐1184 behaves as a cannabinoid receptor antagonist in the guinea‐pig myenteric plexus‐longitudinal muscle preparation.
British Journal of Pharmacology (1998) 125, 1345–1351; doi:10.1038/sj.bjp.0702204</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1038/sj.bjp.0702204</identifier><identifier>PMID: 9863666</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol ; Analgesics - metabolism ; Analgesics - pharmacology ; Animals ; Benzoxazines ; Binding Sites ; Binding, Competitive ; Biological and medical sciences ; Camphanes - metabolism ; Camphanes - pharmacology ; cannabinoid receptor antagonists ; Cannabinoid receptors ; Cyclohexanols - metabolism ; Cyclohexanols - pharmacology ; Dronabinol - analogs & derivatives ; Dronabinol - metabolism ; Dronabinol - pharmacology ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; guinea‐pig brain ; guinea‐pig small intestine ; Intestine, Small - innervation ; Intestine, Small - metabolism ; Intestine, Small - ultrastructure ; Intestine. Mesentery ; Kinetics ; Male ; Morpholines - metabolism ; Morpholines - pharmacology ; Muscle Contraction - drug effects ; Muscle, Smooth - innervation ; myenteric plexus ; Myenteric Plexus - metabolism ; Myenteric Plexus - ultrastructure ; Naphthalenes - metabolism ; Naphthalenes - pharmacology ; Neuromuscular Junction - metabolism ; Neuromuscular Junction - ultrastructure ; O‐1184 ; Piperidines - metabolism ; Piperidines - pharmacology ; Prosencephalon - metabolism ; Prosencephalon - ultrastructure ; Pyrazoles - metabolism ; Pyrazoles - pharmacology ; Receptors, Cannabinoid ; Receptors, Drug - agonists ; Receptors, Drug - antagonists & inhibitors ; Receptors, Drug - metabolism ; Rimonabant ; Tritium ; Vertebrates: digestive system</subject><ispartof>British journal of pharmacology, 1998-11, Vol.125 (6), p.1345-1351</ispartof><rights>1998 British Pharmacological Society</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1998, Nature Publishing Group 1998 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4890-ed5b6ff3fbc29f4303da5657a1034cfd26afc76b53be85bdd691eb7333bb003f3</citedby><cites>FETCH-LOGICAL-c4890-ed5b6ff3fbc29f4303da5657a1034cfd26afc76b53be85bdd691eb7333bb003f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1565708/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1565708/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1632021$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9863666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ross, Ruth A</creatorcontrib><creatorcontrib>Brockie, Heather C</creatorcontrib><creatorcontrib>Fernando, Susanthi R</creatorcontrib><creatorcontrib>Saha, Bijali</creatorcontrib><creatorcontrib>Razdan, Raj K</creatorcontrib><creatorcontrib>Pertwee, Roger G</creatorcontrib><title>Comparison of cannabinoid binding sites in guinea‐pig forebrain and small intestine</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>1
We have investigated the nature of cannabinoid receptors in guinea‐pig small intestine by establishing whether this tissue contains cannabinoid receptors with similar binding properties to those of brain CB1 receptors. The cannabinoids used were the CB1‐selective antagonist SR141716A, the CB2‐selective antagonist SR144528, the novel cannabinoid receptor ligand, 6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol (O‐1184), and the agonists CP55940, which binds equally well to CB1 and CB2 receptors, and WIN55212‐2, which shows marginal CB2 selectivity.
2
[3H]‐CP55940 (1 nm) underwent extensive specific binding both to forebrain membranes (76.3%) and to membranes obtained by sucrose density gradient fractionation of homogenates of myenteric plexus‐longitudinal muscle of guinea‐pig small intestine (65.2%).
3
Its binding capacity (Bmax) was higher in forebrain (4281 fmol mg−1) than in intestinal membranes (2092 fmol mg−1). However, the corresponding KD values were not significantly different from each other (2.29 and 1.75 nm respectively). Nor did the Ki values for its displacement by CP55940, WIN55212‐2, O‐1184, SR141716A and SR144528 from forebrain membranes (0.87, 4.15, 2.85, 5.32 and 371.9 respectively) differ significantly from the corresponding Ki values determined in experiments with intestinal membranes (0.99, 5.03, 3.16, 4.95 and 361.5 nm respectively).
4
The Bmax values of [3H]‐CP55940 and [3H]‐SR141716A in forebrain membranes did not differ significantly from each other (4281 and 5658 fmol mg−1) but were both greater than the Bmax of [3H]‐WIN55212‐2 (2032 fmol mg−1).
5
O‐1184 (10 or 100 nm) produced parallel dextral shifts in the log concentration‐response curves of WIN55212‐2 and CP55940 for inhibition of electrically‐evoked contractions of the myenteric plexus‐longitudinal muscle preparation, its KD values being 0.20 nm (against WIN55212‐2) and 0.89 nm (against CP55940).
6
We conclude that cannabinoid binding sites in guinea‐pig small intestine closely resemble CB1 binding sites of guinea‐pig brain and that O‐1184 behaves as a cannabinoid receptor antagonist in the guinea‐pig myenteric plexus‐longitudinal muscle preparation.
British Journal of Pharmacology (1998) 125, 1345–1351; doi:10.1038/sj.bjp.0702204</description><subject>6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol</subject><subject>Analgesics - metabolism</subject><subject>Analgesics - pharmacology</subject><subject>Animals</subject><subject>Benzoxazines</subject><subject>Binding Sites</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Camphanes - metabolism</subject><subject>Camphanes - pharmacology</subject><subject>cannabinoid receptor antagonists</subject><subject>Cannabinoid receptors</subject><subject>Cyclohexanols - metabolism</subject><subject>Cyclohexanols - pharmacology</subject><subject>Dronabinol - analogs & derivatives</subject><subject>Dronabinol - metabolism</subject><subject>Dronabinol - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>guinea‐pig brain</subject><subject>guinea‐pig small intestine</subject><subject>Intestine, Small - innervation</subject><subject>Intestine, Small - metabolism</subject><subject>Intestine, Small - ultrastructure</subject><subject>Intestine. Mesentery</subject><subject>Kinetics</subject><subject>Male</subject><subject>Morpholines - metabolism</subject><subject>Morpholines - pharmacology</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - innervation</subject><subject>myenteric plexus</subject><subject>Myenteric Plexus - metabolism</subject><subject>Myenteric Plexus - ultrastructure</subject><subject>Naphthalenes - metabolism</subject><subject>Naphthalenes - pharmacology</subject><subject>Neuromuscular Junction - metabolism</subject><subject>Neuromuscular Junction - ultrastructure</subject><subject>O‐1184</subject><subject>Piperidines - metabolism</subject><subject>Piperidines - pharmacology</subject><subject>Prosencephalon - metabolism</subject><subject>Prosencephalon - ultrastructure</subject><subject>Pyrazoles - metabolism</subject><subject>Pyrazoles - pharmacology</subject><subject>Receptors, Cannabinoid</subject><subject>Receptors, Drug - agonists</subject><subject>Receptors, Drug - antagonists & inhibitors</subject><subject>Receptors, Drug - metabolism</subject><subject>Rimonabant</subject><subject>Tritium</subject><subject>Vertebrates: digestive system</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKsvClRtSDohblrGdOM4FCVaFIlWCAz1bY8devErsYO8W9cZP6G_kl-BqowKnnkby-_zejB4hLylsKHD5Nu83ej9voAPGoHlEVrTpRN1ySR-TFQB0NaVSPiXPct4DFLFrz8hZLwUXQqzI1TZOMyafY6iiqwyGgNqH6IeqjMGHXZX9webKh2p39MHi71-3s99VLiarE5ZnDEOVJxzHwhTyUKDn5InDMdsXy1yTq4_n37YX9eWXT5-37y9r08geaju0WjjHnTasdw0HPmAr2g7LZY1xAxPoTCd0y7WVrR4G0VOrO8651gDc8TV5d_Kdj3qyg7HhkHBUc_ITphsV0av_leC_q128VvQuBmQxeLMYpPjjWJZXk8_GjiMGG49ZiR56ydjDIO0Y5VL0BdycQJNizsm6-20oqLvGVN6r0phaGisfXv17wz2-VFT014uO2eDoEgbj819XwRmU7DXhJ-ynH-3NA6Hqw9cLwYrwB3XNsrY</recordid><startdate>199811</startdate><enddate>199811</enddate><creator>Ross, Ruth A</creator><creator>Brockie, Heather C</creator><creator>Fernando, Susanthi R</creator><creator>Saha, Bijali</creator><creator>Razdan, Raj K</creator><creator>Pertwee, Roger G</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199811</creationdate><title>Comparison of cannabinoid binding sites in guinea‐pig forebrain and small intestine</title><author>Ross, Ruth A ; Brockie, Heather C ; Fernando, Susanthi R ; Saha, Bijali ; Razdan, Raj K ; Pertwee, Roger G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4890-ed5b6ff3fbc29f4303da5657a1034cfd26afc76b53be85bdd691eb7333bb003f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol</topic><topic>Analgesics - metabolism</topic><topic>Analgesics - pharmacology</topic><topic>Animals</topic><topic>Benzoxazines</topic><topic>Binding Sites</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Camphanes - metabolism</topic><topic>Camphanes - pharmacology</topic><topic>cannabinoid receptor antagonists</topic><topic>Cannabinoid receptors</topic><topic>Cyclohexanols - metabolism</topic><topic>Cyclohexanols - pharmacology</topic><topic>Dronabinol - analogs & derivatives</topic><topic>Dronabinol - metabolism</topic><topic>Dronabinol - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>guinea‐pig brain</topic><topic>guinea‐pig small intestine</topic><topic>Intestine, Small - innervation</topic><topic>Intestine, Small - metabolism</topic><topic>Intestine, Small - ultrastructure</topic><topic>Intestine. Mesentery</topic><topic>Kinetics</topic><topic>Male</topic><topic>Morpholines - metabolism</topic><topic>Morpholines - pharmacology</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - innervation</topic><topic>myenteric plexus</topic><topic>Myenteric Plexus - metabolism</topic><topic>Myenteric Plexus - ultrastructure</topic><topic>Naphthalenes - metabolism</topic><topic>Naphthalenes - pharmacology</topic><topic>Neuromuscular Junction - metabolism</topic><topic>Neuromuscular Junction - ultrastructure</topic><topic>O‐1184</topic><topic>Piperidines - metabolism</topic><topic>Piperidines - pharmacology</topic><topic>Prosencephalon - metabolism</topic><topic>Prosencephalon - ultrastructure</topic><topic>Pyrazoles - metabolism</topic><topic>Pyrazoles - pharmacology</topic><topic>Receptors, Cannabinoid</topic><topic>Receptors, Drug - agonists</topic><topic>Receptors, Drug - antagonists & inhibitors</topic><topic>Receptors, Drug - metabolism</topic><topic>Rimonabant</topic><topic>Tritium</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ross, Ruth A</creatorcontrib><creatorcontrib>Brockie, Heather C</creatorcontrib><creatorcontrib>Fernando, Susanthi R</creatorcontrib><creatorcontrib>Saha, Bijali</creatorcontrib><creatorcontrib>Razdan, Raj K</creatorcontrib><creatorcontrib>Pertwee, Roger G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ross, Ruth A</au><au>Brockie, Heather C</au><au>Fernando, Susanthi R</au><au>Saha, Bijali</au><au>Razdan, Raj K</au><au>Pertwee, Roger G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of cannabinoid binding sites in guinea‐pig forebrain and small intestine</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>1998-11</date><risdate>1998</risdate><volume>125</volume><issue>6</issue><spage>1345</spage><epage>1351</epage><pages>1345-1351</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>1
We have investigated the nature of cannabinoid receptors in guinea‐pig small intestine by establishing whether this tissue contains cannabinoid receptors with similar binding properties to those of brain CB1 receptors. The cannabinoids used were the CB1‐selective antagonist SR141716A, the CB2‐selective antagonist SR144528, the novel cannabinoid receptor ligand, 6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol (O‐1184), and the agonists CP55940, which binds equally well to CB1 and CB2 receptors, and WIN55212‐2, which shows marginal CB2 selectivity.
2
[3H]‐CP55940 (1 nm) underwent extensive specific binding both to forebrain membranes (76.3%) and to membranes obtained by sucrose density gradient fractionation of homogenates of myenteric plexus‐longitudinal muscle of guinea‐pig small intestine (65.2%).
3
Its binding capacity (Bmax) was higher in forebrain (4281 fmol mg−1) than in intestinal membranes (2092 fmol mg−1). However, the corresponding KD values were not significantly different from each other (2.29 and 1.75 nm respectively). Nor did the Ki values for its displacement by CP55940, WIN55212‐2, O‐1184, SR141716A and SR144528 from forebrain membranes (0.87, 4.15, 2.85, 5.32 and 371.9 respectively) differ significantly from the corresponding Ki values determined in experiments with intestinal membranes (0.99, 5.03, 3.16, 4.95 and 361.5 nm respectively).
4
The Bmax values of [3H]‐CP55940 and [3H]‐SR141716A in forebrain membranes did not differ significantly from each other (4281 and 5658 fmol mg−1) but were both greater than the Bmax of [3H]‐WIN55212‐2 (2032 fmol mg−1).
5
O‐1184 (10 or 100 nm) produced parallel dextral shifts in the log concentration‐response curves of WIN55212‐2 and CP55940 for inhibition of electrically‐evoked contractions of the myenteric plexus‐longitudinal muscle preparation, its KD values being 0.20 nm (against WIN55212‐2) and 0.89 nm (against CP55940).
6
We conclude that cannabinoid binding sites in guinea‐pig small intestine closely resemble CB1 binding sites of guinea‐pig brain and that O‐1184 behaves as a cannabinoid receptor antagonist in the guinea‐pig myenteric plexus‐longitudinal muscle preparation.
British Journal of Pharmacology (1998) 125, 1345–1351; doi:10.1038/sj.bjp.0702204</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9863666</pmid><doi>10.1038/sj.bjp.0702204</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | British journal of pharmacology, 1998-11, Vol.125 (6), p.1345-1351 |
| issn | 0007-1188 1476-5381 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1565708 |
| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | 6′‐azidohex‐2′‐yne‐Δ8‐tetrahydrocannabinol Analgesics - metabolism Analgesics - pharmacology Animals Benzoxazines Binding Sites Binding, Competitive Biological and medical sciences Camphanes - metabolism Camphanes - pharmacology cannabinoid receptor antagonists Cannabinoid receptors Cyclohexanols - metabolism Cyclohexanols - pharmacology Dronabinol - analogs & derivatives Dronabinol - metabolism Dronabinol - pharmacology Fundamental and applied biological sciences. Psychology Guinea Pigs guinea‐pig brain guinea‐pig small intestine Intestine, Small - innervation Intestine, Small - metabolism Intestine, Small - ultrastructure Intestine. Mesentery Kinetics Male Morpholines - metabolism Morpholines - pharmacology Muscle Contraction - drug effects Muscle, Smooth - innervation myenteric plexus Myenteric Plexus - metabolism Myenteric Plexus - ultrastructure Naphthalenes - metabolism Naphthalenes - pharmacology Neuromuscular Junction - metabolism Neuromuscular Junction - ultrastructure O‐1184 Piperidines - metabolism Piperidines - pharmacology Prosencephalon - metabolism Prosencephalon - ultrastructure Pyrazoles - metabolism Pyrazoles - pharmacology Receptors, Cannabinoid Receptors, Drug - agonists Receptors, Drug - antagonists & inhibitors Receptors, Drug - metabolism Rimonabant Tritium Vertebrates: digestive system |
| title | Comparison of cannabinoid binding sites in guinea‐pig forebrain and small intestine |
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