Functional Splice Variants of the Type II G Protein-Coupled Receptor (VPAC2) for Vasoactive Intestinal Peptide in Mouse and Human Lymphocytes
: A PCR‐based search for splice variants of the VPAC2 G protein–coupled receptor for vasoactive intestinal peptide (VIP) revealed: (a) a short‐deletion variant in mouse lymphocytes termed VPAC2de367–380, that lacks 14 amino acids in the seventh transmembrane domain, and (b) a long‐deletion variant...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2006-07, Vol.1070 (1), p.422-426 |
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| container_title | Annals of the New York Academy of Sciences |
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| creator | MILLER, ALLISON L. VERMA, DEEPTI GRINNINGER, CAROLA HUANG, MEI-CHUAN GOETZL, EDWARD J. |
| description | : A PCR‐based search for splice variants of the VPAC2 G protein–coupled receptor for vasoactive intestinal peptide (VIP) revealed: (a) a short‐deletion variant in mouse lymphocytes termed VPAC2de367–380, that lacks 14 amino acids in the seventh transmembrane domain, and (b) a long‐deletion variant in human lymphocytes termed VPAC2de325–438(i325–334), that lacks 114 amino acids beginning with the carboxyl‐terminal end of the third cytoplasmic loop and has 10 new carboxy‐terminal amino acids. VPAC2de367–380 binds VIP normally, but shows reduced VIP‐evoked signaling and effects on immune functions, whereas VPAC2de325–438(i325–334) shows reduced binding affinity for VIP and a complex pattern of functional differences. These splice variants may modify the immunoregulatory contributions of the VIP–VPAC2 axis. |
| doi_str_mv | 10.1196/annals.1317.055 |
| format | Article |
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VPAC2de367–380 binds VIP normally, but shows reduced VIP‐evoked signaling and effects on immune functions, whereas VPAC2de325–438(i325–334) shows reduced binding affinity for VIP and a complex pattern of functional differences. These splice variants may modify the immunoregulatory contributions of the VIP–VPAC2 axis.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>DOI: 10.1196/annals.1317.055</identifier><identifier>PMID: 16888203</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Amino Acid Sequence ; Animals ; cytokine ; Genetic Variation - genetics ; Humans ; immunity ; Lymphocytes - metabolism ; Mice ; Molecular Sequence Data ; neuropeptide ; Receptors, Vasoactive Intestinal Peptide, Type II - chemistry ; Receptors, Vasoactive Intestinal Peptide, Type II - genetics ; RNA Splicing - genetics ; T cell ; Vasoactive Intestinal Peptide - metabolism</subject><ispartof>Annals of the New York Academy of Sciences, 2006-07, Vol.1070 (1), p.422-426</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5705-a5324664257e6f231e7cc4897810a6de26870300c861742d7d1bb36ab625f9493</citedby><cites>FETCH-LOGICAL-c5705-a5324664257e6f231e7cc4897810a6de26870300c861742d7d1bb36ab625f9493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1196%2Fannals.1317.055$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1196%2Fannals.1317.055$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,778,782,883,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16888203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MILLER, ALLISON L.</creatorcontrib><creatorcontrib>VERMA, DEEPTI</creatorcontrib><creatorcontrib>GRINNINGER, CAROLA</creatorcontrib><creatorcontrib>HUANG, MEI-CHUAN</creatorcontrib><creatorcontrib>GOETZL, EDWARD J.</creatorcontrib><title>Functional Splice Variants of the Type II G Protein-Coupled Receptor (VPAC2) for Vasoactive Intestinal Peptide in Mouse and Human Lymphocytes</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>: A PCR‐based search for splice variants of the VPAC2 G protein–coupled receptor for vasoactive intestinal peptide (VIP) revealed: (a) a short‐deletion variant in mouse lymphocytes termed VPAC2de367–380, that lacks 14 amino acids in the seventh transmembrane domain, and (b) a long‐deletion variant in human lymphocytes termed VPAC2de325–438(i325–334), that lacks 114 amino acids beginning with the carboxyl‐terminal end of the third cytoplasmic loop and has 10 new carboxy‐terminal amino acids. VPAC2de367–380 binds VIP normally, but shows reduced VIP‐evoked signaling and effects on immune functions, whereas VPAC2de325–438(i325–334) shows reduced binding affinity for VIP and a complex pattern of functional differences. These splice variants may modify the immunoregulatory contributions of the VIP–VPAC2 axis.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>cytokine</subject><subject>Genetic Variation - genetics</subject><subject>Humans</subject><subject>immunity</subject><subject>Lymphocytes - metabolism</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>neuropeptide</subject><subject>Receptors, Vasoactive Intestinal Peptide, Type II - chemistry</subject><subject>Receptors, Vasoactive Intestinal Peptide, Type II - genetics</subject><subject>RNA Splicing - genetics</subject><subject>T cell</subject><subject>Vasoactive Intestinal Peptide - metabolism</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v0zAYhyMEYt3gzA35hOCQzn9iO7kgVRXrCu2oWClwslznDTWkdoiTjX4IvjMurQacdrIlP--j9-dfkjwjeEhIIc61c7oOQ8KIHGLOHyQDIrMiFYLRh8kAYynTvKDsJDkN4RvGhOaZfJycEJHnOcVskPy66J3prI8adN3U1gBa6dZq1wXkK9RtAC13DaDpFE3QovUdWJeOfd_UUKIPYKDpfIterhajMX2Fqnhf6eB1VN7EIddB6OzevYigLQFZh-a-D4C0K9Flv9UOzXbbZuPNLrJPkkdVzANPj-dZ8vHizXJ8mc7eT6bj0Sw1XGKeas5oJkRGuQRRUUZAGpPlhcwJ1qIEKnKJGcYmF_E7aClLsl4zodeC8qrICnaWvD54m369hdKA61pdq6a1W93ulNdW_f_i7EZ99TeKcC4F3wteHAWt_9HHkGprg4G61g5iPBUXIEzGJe4DKaYc5wWJ4PkBNK0PoYXqbhuC1b5rdeha7btWses48fzfEH_5Y7kRYAfg1tawu8-nrr6Mrv9o08OUDR38vJvS7XclJJNcfbqaKCrfvvu8nC_UnP0GGI_Gjw</recordid><startdate>200607</startdate><enddate>200607</enddate><creator>MILLER, ALLISON L.</creator><creator>VERMA, DEEPTI</creator><creator>GRINNINGER, CAROLA</creator><creator>HUANG, MEI-CHUAN</creator><creator>GOETZL, EDWARD J.</creator><general>Blackwell Publishing Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200607</creationdate><title>Functional Splice Variants of the Type II G Protein-Coupled Receptor (VPAC2) for Vasoactive Intestinal Peptide in Mouse and Human Lymphocytes</title><author>MILLER, ALLISON L. ; VERMA, DEEPTI ; GRINNINGER, CAROLA ; HUANG, MEI-CHUAN ; GOETZL, EDWARD J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5705-a5324664257e6f231e7cc4897810a6de26870300c861742d7d1bb36ab625f9493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>cytokine</topic><topic>Genetic Variation - genetics</topic><topic>Humans</topic><topic>immunity</topic><topic>Lymphocytes - metabolism</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>neuropeptide</topic><topic>Receptors, Vasoactive Intestinal Peptide, Type II - chemistry</topic><topic>Receptors, Vasoactive Intestinal Peptide, Type II - genetics</topic><topic>RNA Splicing - genetics</topic><topic>T cell</topic><topic>Vasoactive Intestinal Peptide - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MILLER, ALLISON L.</creatorcontrib><creatorcontrib>VERMA, DEEPTI</creatorcontrib><creatorcontrib>GRINNINGER, CAROLA</creatorcontrib><creatorcontrib>HUANG, MEI-CHUAN</creatorcontrib><creatorcontrib>GOETZL, EDWARD J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MILLER, ALLISON L.</au><au>VERMA, DEEPTI</au><au>GRINNINGER, CAROLA</au><au>HUANG, MEI-CHUAN</au><au>GOETZL, EDWARD J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Splice Variants of the Type II G Protein-Coupled Receptor (VPAC2) for Vasoactive Intestinal Peptide in Mouse and Human Lymphocytes</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2006-07</date><risdate>2006</risdate><volume>1070</volume><issue>1</issue><spage>422</spage><epage>426</epage><pages>422-426</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>: A PCR‐based search for splice variants of the VPAC2 G protein–coupled receptor for vasoactive intestinal peptide (VIP) revealed: (a) a short‐deletion variant in mouse lymphocytes termed VPAC2de367–380, that lacks 14 amino acids in the seventh transmembrane domain, and (b) a long‐deletion variant in human lymphocytes termed VPAC2de325–438(i325–334), that lacks 114 amino acids beginning with the carboxyl‐terminal end of the third cytoplasmic loop and has 10 new carboxy‐terminal amino acids. VPAC2de367–380 binds VIP normally, but shows reduced VIP‐evoked signaling and effects on immune functions, whereas VPAC2de325–438(i325–334) shows reduced binding affinity for VIP and a complex pattern of functional differences. These splice variants may modify the immunoregulatory contributions of the VIP–VPAC2 axis.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>16888203</pmid><doi>10.1196/annals.1317.055</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acid Sequence Animals cytokine Genetic Variation - genetics Humans immunity Lymphocytes - metabolism Mice Molecular Sequence Data neuropeptide Receptors, Vasoactive Intestinal Peptide, Type II - chemistry Receptors, Vasoactive Intestinal Peptide, Type II - genetics RNA Splicing - genetics T cell Vasoactive Intestinal Peptide - metabolism |
| title | Functional Splice Variants of the Type II G Protein-Coupled Receptor (VPAC2) for Vasoactive Intestinal Peptide in Mouse and Human Lymphocytes |
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