Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome
Postweaning multisystemic wasting syndrome (PMWS), an apparently new disease, has been recognized in swine herds in western Canada. Young pigs with this disease have progressive weight loss, tachypnea, dyspnea, and jaundice, accompanied by interstitial pneumonia, lymphadenopathy, hepatitis, and neph...
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| Veröffentlicht in: | Canadian veterinary journal 1998, Vol.39 (1), p.44-51 |
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| creator | Ellis, J Hassard, L Clark, E Harding, J Allan, G Willson, P Strokappe, J Martin, K McNeilly, F Meehan, B |
| description | Postweaning multisystemic wasting syndrome (PMWS), an apparently new disease, has been recognized in swine herds in western Canada. Young pigs with this disease have progressive weight loss, tachypnea, dyspnea, and jaundice, accompanied by interstitial pneumonia, lymphadenopathy, hepatitis, and nephritis. We examined more than 400 pigs from more than 70 herds in Alberta, Saskatchewan, and Manitoba with cases of PMWS. A small virus was isolated from a range of tissues from 8 of 8 affected pigs examined. The agent was identified as a circovirus-like virus using electron microscopy, immunohistochemical staining with porcine and rabbit immune serum, and in situ hybridization. Immunohistochemical examination of tissues from more than 100 affected pigs has revealed widespread viral antigen, often contained in circovirus-like inclusion bodies, in lesions from numerous organs. Although Koch's postulates remain to be fulfilled, these results demonstrate a high degree of association between the presence of the circovirus-like virus and PMWS in affected swine. |
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Young pigs with this disease have progressive weight loss, tachypnea, dyspnea, and jaundice, accompanied by interstitial pneumonia, lymphadenopathy, hepatitis, and nephritis. We examined more than 400 pigs from more than 70 herds in Alberta, Saskatchewan, and Manitoba with cases of PMWS. A small virus was isolated from a range of tissues from 8 of 8 affected pigs examined. The agent was identified as a circovirus-like virus using electron microscopy, immunohistochemical staining with porcine and rabbit immune serum, and in situ hybridization. Immunohistochemical examination of tissues from more than 100 affected pigs has revealed widespread viral antigen, often contained in circovirus-like inclusion bodies, in lesions from numerous organs. Although Koch's postulates remain to be fulfilled, these results demonstrate a high degree of association between the presence of the circovirus-like virus and PMWS in affected swine.</description><identifier>ISSN: 0008-5286</identifier><identifier>PMID: 9442952</identifier><language>eng</language><publisher>Canada</publisher><subject>Alberta - epidemiology ; animal tissues ; Animals ; Antibodies, Viral - analysis ; Antibodies, Viral - blood ; antigenic variation ; Antigens, Viral - analysis ; Antigens, Viral - blood ; Circoviridae - immunology ; Circoviridae - isolation & purification ; Circoviridae - ultrastructure ; Circoviridae Infections - epidemiology ; Circoviridae Infections - pathology ; Circoviridae Infections - veterinary ; Female ; Immunohistochemistry ; In Situ Hybridization - veterinary ; isolation ; Kidney - pathology ; Kidney - virology ; lesions ; Lung - pathology ; Lung - virology ; Lymphoid Tissue - pathology ; Lymphoid Tissue - virology ; Male ; Manitoba - epidemiology ; Microscopy, Electron - methods ; Microscopy, Electron - veterinary ; Porcine circovirus ; Rabbits ; Saskatchewan - epidemiology ; Swine ; swine diseases ; Swine Diseases - epidemiology ; Swine Diseases - pathology ; Swine Diseases - virology ; viral antigens ; Wasting Syndrome - pathology ; Wasting Syndrome - veterinary ; Wasting Syndrome - virology</subject><ispartof>Canadian veterinary journal, 1998, Vol.39 (1), p.44-51</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1539838/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1539838/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9442952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ellis, J</creatorcontrib><creatorcontrib>Hassard, L</creatorcontrib><creatorcontrib>Clark, E</creatorcontrib><creatorcontrib>Harding, J</creatorcontrib><creatorcontrib>Allan, G</creatorcontrib><creatorcontrib>Willson, P</creatorcontrib><creatorcontrib>Strokappe, J</creatorcontrib><creatorcontrib>Martin, K</creatorcontrib><creatorcontrib>McNeilly, F</creatorcontrib><creatorcontrib>Meehan, B</creatorcontrib><title>Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome</title><title>Canadian veterinary journal</title><addtitle>Can Vet J</addtitle><description>Postweaning multisystemic wasting syndrome (PMWS), an apparently new disease, has been recognized in swine herds in western Canada. Young pigs with this disease have progressive weight loss, tachypnea, dyspnea, and jaundice, accompanied by interstitial pneumonia, lymphadenopathy, hepatitis, and nephritis. We examined more than 400 pigs from more than 70 herds in Alberta, Saskatchewan, and Manitoba with cases of PMWS. A small virus was isolated from a range of tissues from 8 of 8 affected pigs examined. The agent was identified as a circovirus-like virus using electron microscopy, immunohistochemical staining with porcine and rabbit immune serum, and in situ hybridization. Immunohistochemical examination of tissues from more than 100 affected pigs has revealed widespread viral antigen, often contained in circovirus-like inclusion bodies, in lesions from numerous organs. Although Koch's postulates remain to be fulfilled, these results demonstrate a high degree of association between the presence of the circovirus-like virus and PMWS in affected swine.</description><subject>Alberta - epidemiology</subject><subject>animal tissues</subject><subject>Animals</subject><subject>Antibodies, Viral - analysis</subject><subject>Antibodies, Viral - blood</subject><subject>antigenic variation</subject><subject>Antigens, Viral - analysis</subject><subject>Antigens, Viral - blood</subject><subject>Circoviridae - immunology</subject><subject>Circoviridae - isolation & purification</subject><subject>Circoviridae - ultrastructure</subject><subject>Circoviridae Infections - epidemiology</subject><subject>Circoviridae Infections - pathology</subject><subject>Circoviridae Infections - veterinary</subject><subject>Female</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization - veterinary</subject><subject>isolation</subject><subject>Kidney - pathology</subject><subject>Kidney - virology</subject><subject>lesions</subject><subject>Lung - pathology</subject><subject>Lung - virology</subject><subject>Lymphoid Tissue - pathology</subject><subject>Lymphoid Tissue - virology</subject><subject>Male</subject><subject>Manitoba - epidemiology</subject><subject>Microscopy, Electron - methods</subject><subject>Microscopy, Electron - veterinary</subject><subject>Porcine circovirus</subject><subject>Rabbits</subject><subject>Saskatchewan - epidemiology</subject><subject>Swine</subject><subject>swine diseases</subject><subject>Swine Diseases - epidemiology</subject><subject>Swine Diseases - pathology</subject><subject>Swine Diseases - virology</subject><subject>viral antigens</subject><subject>Wasting Syndrome - pathology</subject><subject>Wasting Syndrome - veterinary</subject><subject>Wasting Syndrome - virology</subject><issn>0008-5286</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkF1LwzAUhnuhzDn9CWKuvCukSZsmN4IMPwaCF7o7IWTpSRdpm9mTbuzf27EhenXgPO95XjhnyZRSKtOCSXGRXCJ-UcpUJugkmag8Z6pg0-RzgaEx0YeOBEes723Y-n5A4vrQkgZwJHhAG18j2fm4JpuAcQem811N2qGJHvcYofWW7AzGwxb3XTWew1Vy7kyDcH2as2T59Pgxf0lf354X84fX1DFZxNQpY6tyVRbgILcsrypJqbUZSOCUc1ooyktRGVNaBsqJlVAy4zazUgpgouKz5P7o3QyrFioLXexNoze9b02_18F4_Z90fq3rsNVZwZXkchTcnQR9-B4Ao249Wmga00EYUJdKiCLnagze_G36rTj9c-S3R-5M0KbuPerlO6MZp1mpGFcl_wFdOX3u</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Ellis, J</creator><creator>Hassard, L</creator><creator>Clark, E</creator><creator>Harding, J</creator><creator>Allan, G</creator><creator>Willson, P</creator><creator>Strokappe, J</creator><creator>Martin, K</creator><creator>McNeilly, F</creator><creator>Meehan, B</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1998</creationdate><title>Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome</title><author>Ellis, J ; Hassard, L ; Clark, E ; Harding, J ; Allan, G ; Willson, P ; Strokappe, J ; Martin, K ; McNeilly, F ; Meehan, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f285t-f9acd7b75efe4c24dd800cc1e8e30330590376daa7c2e9f6b69813c1c886e26d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alberta - epidemiology</topic><topic>animal tissues</topic><topic>Animals</topic><topic>Antibodies, Viral - analysis</topic><topic>Antibodies, Viral - blood</topic><topic>antigenic variation</topic><topic>Antigens, Viral - analysis</topic><topic>Antigens, Viral - blood</topic><topic>Circoviridae - immunology</topic><topic>Circoviridae - isolation & purification</topic><topic>Circoviridae - ultrastructure</topic><topic>Circoviridae Infections - epidemiology</topic><topic>Circoviridae Infections - pathology</topic><topic>Circoviridae Infections - veterinary</topic><topic>Female</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization - veterinary</topic><topic>isolation</topic><topic>Kidney - pathology</topic><topic>Kidney - virology</topic><topic>lesions</topic><topic>Lung - pathology</topic><topic>Lung - virology</topic><topic>Lymphoid Tissue - pathology</topic><topic>Lymphoid Tissue - virology</topic><topic>Male</topic><topic>Manitoba - epidemiology</topic><topic>Microscopy, Electron - methods</topic><topic>Microscopy, Electron - veterinary</topic><topic>Porcine circovirus</topic><topic>Rabbits</topic><topic>Saskatchewan - epidemiology</topic><topic>Swine</topic><topic>swine diseases</topic><topic>Swine Diseases - epidemiology</topic><topic>Swine Diseases - pathology</topic><topic>Swine Diseases - virology</topic><topic>viral antigens</topic><topic>Wasting Syndrome - pathology</topic><topic>Wasting Syndrome - veterinary</topic><topic>Wasting Syndrome - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ellis, J</creatorcontrib><creatorcontrib>Hassard, L</creatorcontrib><creatorcontrib>Clark, E</creatorcontrib><creatorcontrib>Harding, J</creatorcontrib><creatorcontrib>Allan, G</creatorcontrib><creatorcontrib>Willson, P</creatorcontrib><creatorcontrib>Strokappe, J</creatorcontrib><creatorcontrib>Martin, K</creatorcontrib><creatorcontrib>McNeilly, F</creatorcontrib><creatorcontrib>Meehan, B</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Canadian veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ellis, J</au><au>Hassard, L</au><au>Clark, E</au><au>Harding, J</au><au>Allan, G</au><au>Willson, P</au><au>Strokappe, J</au><au>Martin, K</au><au>McNeilly, F</au><au>Meehan, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome</atitle><jtitle>Canadian veterinary journal</jtitle><addtitle>Can Vet J</addtitle><date>1998</date><risdate>1998</risdate><volume>39</volume><issue>1</issue><spage>44</spage><epage>51</epage><pages>44-51</pages><issn>0008-5286</issn><abstract>Postweaning multisystemic wasting syndrome (PMWS), an apparently new disease, has been recognized in swine herds in western Canada. Young pigs with this disease have progressive weight loss, tachypnea, dyspnea, and jaundice, accompanied by interstitial pneumonia, lymphadenopathy, hepatitis, and nephritis. We examined more than 400 pigs from more than 70 herds in Alberta, Saskatchewan, and Manitoba with cases of PMWS. A small virus was isolated from a range of tissues from 8 of 8 affected pigs examined. The agent was identified as a circovirus-like virus using electron microscopy, immunohistochemical staining with porcine and rabbit immune serum, and in situ hybridization. Immunohistochemical examination of tissues from more than 100 affected pigs has revealed widespread viral antigen, often contained in circovirus-like inclusion bodies, in lesions from numerous organs. Although Koch's postulates remain to be fulfilled, these results demonstrate a high degree of association between the presence of the circovirus-like virus and PMWS in affected swine.</abstract><cop>Canada</cop><pmid>9442952</pmid><tpages>8</tpages></addata></record> |
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| ispartof | Canadian veterinary journal, 1998, Vol.39 (1), p.44-51 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Alberta - epidemiology animal tissues Animals Antibodies, Viral - analysis Antibodies, Viral - blood antigenic variation Antigens, Viral - analysis Antigens, Viral - blood Circoviridae - immunology Circoviridae - isolation & purification Circoviridae - ultrastructure Circoviridae Infections - epidemiology Circoviridae Infections - pathology Circoviridae Infections - veterinary Female Immunohistochemistry In Situ Hybridization - veterinary isolation Kidney - pathology Kidney - virology lesions Lung - pathology Lung - virology Lymphoid Tissue - pathology Lymphoid Tissue - virology Male Manitoba - epidemiology Microscopy, Electron - methods Microscopy, Electron - veterinary Porcine circovirus Rabbits Saskatchewan - epidemiology Swine swine diseases Swine Diseases - epidemiology Swine Diseases - pathology Swine Diseases - virology viral antigens Wasting Syndrome - pathology Wasting Syndrome - veterinary Wasting Syndrome - virology |
| title | Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome |
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