PPD-induced viral antibody production in human blood lymphocytes
The ability of purified protein derivative of tuberculin (PPD) to induce polyclonal antibody production in cultures of human blood lymphocytes was studied. IgG and IgM were determined by the enzyme-linked immunosorbent assay (Elisa). PPD induced both IgM and IgG production, with a predominance of Ig...
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| Veröffentlicht in: | Clinical and experimental immunology 1980-11, Vol.42 (2), p.364-369 |
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| container_title | Clinical and experimental immunology |
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| creator | Biberfeld, G Forsgren, M Von Stedingk, L V Arneborn, P |
| description | The ability of purified protein derivative of tuberculin (PPD) to induce polyclonal antibody production in cultures of human blood lymphocytes was studied. IgG and IgM were determined by the enzyme-linked immunosorbent assay (Elisa). PPD induced both IgM and IgG production, with a predominance of IgM. PPD usually stimulated a stronger IgM response but a weaker IgG response than did pokeweed mitogen (PWM). Supernatants of PPD- or PWM-stimulated lymphocyte cultures were tested for antibodies to morbilli, rubella and herpes simplex by Elisa. PPD as well as PWM induced viral antibody production in lymphocytes of donors who had serum antibodies to the corresponding viral antigens. Production of viral antibodies of IgG class but not of IgM class was demonstrated. The PPD-induced antibody response was T cell-dependent. |
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IgG and IgM were determined by the enzyme-linked immunosorbent assay (Elisa). PPD induced both IgM and IgG production, with a predominance of IgM. PPD usually stimulated a stronger IgM response but a weaker IgG response than did pokeweed mitogen (PWM). Supernatants of PPD- or PWM-stimulated lymphocyte cultures were tested for antibodies to morbilli, rubella and herpes simplex by Elisa. PPD as well as PWM induced viral antibody production in lymphocytes of donors who had serum antibodies to the corresponding viral antigens. Production of viral antibodies of IgG class but not of IgM class was demonstrated. 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IgG and IgM were determined by the enzyme-linked immunosorbent assay (Elisa). PPD induced both IgM and IgG production, with a predominance of IgM. PPD usually stimulated a stronger IgM response but a weaker IgG response than did pokeweed mitogen (PWM). Supernatants of PPD- or PWM-stimulated lymphocyte cultures were tested for antibodies to morbilli, rubella and herpes simplex by Elisa. PPD as well as PWM induced viral antibody production in lymphocytes of donors who had serum antibodies to the corresponding viral antigens. Production of viral antibodies of IgG class but not of IgM class was demonstrated. The PPD-induced antibody response was T cell-dependent.</description><subject>Adult</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin M - biosynthesis</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pokeweed Mitogens - pharmacology</subject><subject>Tuberculin - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE9LxDAQxYMo67r6EYScvBWS5k-biyirq8KCe9BzSCepG2mT2rQL_fYWXERPj5k3_N7wTtCSMimyPOfqFC0JISpTlPBzdJHS5zxKKfMFWhS8oILKJbrb7R4yH-wIzuKD702DTRh8Fe2Euz7O-8HHgH3A-7E1AVdNjBY3U9vtI0yDS5forDZNcldHXaH3zePb-jnbvj69rO-3WZdLOmRgSmug4qCMUnlFmADBBBfgQNQOnJSEcytqwYkjtpDCqZKVlWAFEEkEsBW6_eF2Y9U6Cy4M87O6631r-klH4_V_J_i9_ogHTWcGpfkMuDkC-vg1ujTo1idwTWOCi2PSheCSUFrOh9d_k34jjp2xb1k6aqg</recordid><startdate>19801101</startdate><enddate>19801101</enddate><creator>Biberfeld, G</creator><creator>Forsgren, M</creator><creator>Von Stedingk, L V</creator><creator>Arneborn, P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19801101</creationdate><title>PPD-induced viral antibody production in human blood lymphocytes</title><author>Biberfeld, G ; Forsgren, M ; Von Stedingk, L V ; Arneborn, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p261t-ca8dacb4c9a992b035c53545cec5fece66044d5f540e0d765e9838b537c0605c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Adult</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin M - biosynthesis</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pokeweed Mitogens - pharmacology</topic><topic>Tuberculin - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biberfeld, G</creatorcontrib><creatorcontrib>Forsgren, M</creatorcontrib><creatorcontrib>Von Stedingk, L V</creatorcontrib><creatorcontrib>Arneborn, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biberfeld, G</au><au>Forsgren, M</au><au>Von Stedingk, L V</au><au>Arneborn, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PPD-induced viral antibody production in human blood lymphocytes</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1980-11-01</date><risdate>1980</risdate><volume>42</volume><issue>2</issue><spage>364</spage><epage>369</epage><pages>364-369</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><abstract>The ability of purified protein derivative of tuberculin (PPD) to induce polyclonal antibody production in cultures of human blood lymphocytes was studied. IgG and IgM were determined by the enzyme-linked immunosorbent assay (Elisa). PPD induced both IgM and IgG production, with a predominance of IgM. PPD usually stimulated a stronger IgM response but a weaker IgG response than did pokeweed mitogen (PWM). Supernatants of PPD- or PWM-stimulated lymphocyte cultures were tested for antibodies to morbilli, rubella and herpes simplex by Elisa. PPD as well as PWM induced viral antibody production in lymphocytes of donors who had serum antibodies to the corresponding viral antigens. Production of viral antibodies of IgG class but not of IgM class was demonstrated. The PPD-induced antibody response was T cell-dependent.</abstract><cop>England</cop><pmid>7471516</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Clinical and experimental immunology, 1980-11, Vol.42 (2), p.364-369 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adult Antibodies, Viral - biosynthesis Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G - biosynthesis Immunoglobulin M - biosynthesis Lymphocyte Activation Lymphocytes - immunology Male Middle Aged Pokeweed Mitogens - pharmacology Tuberculin - immunology |
| title | PPD-induced viral antibody production in human blood lymphocytes |
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