Cross-reactivity of anti-DNA antibodies with proteoglycans
Ten sera of patients with systemic lupus erythematosus (SLE) were tested in an enzyme linked immunosorbent assay for their ability to react with glycosaminoglycans, constituents of proteoglycans, in relation to their anti-DNA reactivity. The SLE sera reacted with hyaluronic acid and chondroitin sulp...
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| Veröffentlicht in: | Clinical and experimental immunology 1984-03, Vol.55 (3), p.502-508 |
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| container_title | Clinical and experimental immunology |
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| creator | FAABER, P CAPEL, P. J. A RIJKE, G. P. M VIERWINDEN, G VAN DE PUTTE, L. B. A KOENE, R. A. P |
| description | Ten sera of patients with systemic lupus erythematosus (SLE) were tested in an enzyme linked immunosorbent assay for their ability to react with glycosaminoglycans, constituents of proteoglycans, in relation to their anti-DNA reactivity. The SLE sera reacted with hyaluronic acid and chondroitin sulphate and this reactivity correlated with the anti-DNA activity of these sera. By contrast, sera obtained from patients with other autoimmune diseases or normal sera lacked any of these reactivities. Anti-DNA antibodies purified by affinity chromatography with either oligo dT cellulose or Cibracon blue F3Ga Sepharose reacted with DNA as well as with hyaluronic acid. The cross-reactivity of anti-DNA antibodies could be confirmed by the reaction of a mouse monoclonal anti-DNA antibody with DNA, hyaluronic acid, and chondroitin sulphate. This pattern of cross-reactivities of anti-DNA antibodies suggests that several compounds can function as antigenic targets for these antibodies provided that their structures contain repeating negatively charged groups. |
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The cross-reactivity of anti-DNA antibodies could be confirmed by the reaction of a mouse monoclonal anti-DNA antibody with DNA, hyaluronic acid, and chondroitin sulphate. This pattern of cross-reactivities of anti-DNA antibodies suggests that several compounds can function as antigenic targets for these antibodies provided that their structures contain repeating negatively charged groups.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>PMID: 6608423</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Antibodies, Antinuclear - analysis ; Antibodies, Antinuclear - immunology ; Antibodies, immunoglobulins ; Antibodies, Monoclonal - immunology ; Biological and medical sciences ; Cross Reactions ; DNA - immunology ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. 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Anti-DNA antibodies purified by affinity chromatography with either oligo dT cellulose or Cibracon blue F3Ga Sepharose reacted with DNA as well as with hyaluronic acid. The cross-reactivity of anti-DNA antibodies could be confirmed by the reaction of a mouse monoclonal anti-DNA antibody with DNA, hyaluronic acid, and chondroitin sulphate. This pattern of cross-reactivities of anti-DNA antibodies suggests that several compounds can function as antigenic targets for these antibodies provided that their structures contain repeating negatively charged groups.</description><subject>Antibodies, Antinuclear - analysis</subject><subject>Antibodies, Antinuclear - immunology</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>DNA - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycosaminoglycans - immunology</subject><subject>Humans</subject><subject>Hyaluronic Acid - immunology</subject><subject>Lupus Erythematosus, Systemic - immunology</subject><subject>Molecular immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEUhYMotVZ_gjALcRfIa2YSF0KpTyi60fVwJ5O0kelkTFKl_95Wh6IrV_dczuHjcA7QmPIix4wJdYjGhBCFFSXiGJ3E-LZ9i6JgIzQqCiIF42N0NQs-RhwM6OQ-XNpk3mbQJYdvnqbfovaNMzH7dGmZ9cEn4xftRkMXT9GRhTaas-FO0Ovd7cvsAc-f7x9n0znuOaMJi7omQjHKwZq8UTmUJWWqNECbmlkplVVNWe8EF8RKA0KqhijNtRVUGMon6PqH26_rlWm06VKAtuqDW0HYVB5c9dfp3LJa-I-K5jxXtNgCLgdA8O9rE1O1clGbtoXO-HWsJFGqJIT9G6Rc5pzyXaXz35X2XYZZt_7F4EPU0NoAnXZxH5OyJDvUFxMAgh0</recordid><startdate>19840301</startdate><enddate>19840301</enddate><creator>FAABER, P</creator><creator>CAPEL, P. J. 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P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cross-reactivity of anti-DNA antibodies with proteoglycans</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1984-03-01</date><risdate>1984</risdate><volume>55</volume><issue>3</issue><spage>502</spage><epage>508</epage><pages>502-508</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>Ten sera of patients with systemic lupus erythematosus (SLE) were tested in an enzyme linked immunosorbent assay for their ability to react with glycosaminoglycans, constituents of proteoglycans, in relation to their anti-DNA reactivity. The SLE sera reacted with hyaluronic acid and chondroitin sulphate and this reactivity correlated with the anti-DNA activity of these sera. By contrast, sera obtained from patients with other autoimmune diseases or normal sera lacked any of these reactivities. Anti-DNA antibodies purified by affinity chromatography with either oligo dT cellulose or Cibracon blue F3Ga Sepharose reacted with DNA as well as with hyaluronic acid. The cross-reactivity of anti-DNA antibodies could be confirmed by the reaction of a mouse monoclonal anti-DNA antibody with DNA, hyaluronic acid, and chondroitin sulphate. This pattern of cross-reactivities of anti-DNA antibodies suggests that several compounds can function as antigenic targets for these antibodies provided that their structures contain repeating negatively charged groups.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>6608423</pmid><tpages>7</tpages></addata></record> |
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| ispartof | Clinical and experimental immunology, 1984-03, Vol.55 (3), p.502-508 |
| issn | 0009-9104 1365-2249 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1535916 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Antibodies, Antinuclear - analysis Antibodies, Antinuclear - immunology Antibodies, immunoglobulins Antibodies, Monoclonal - immunology Biological and medical sciences Cross Reactions DNA - immunology Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Fundamental immunology Glycosaminoglycans - immunology Humans Hyaluronic Acid - immunology Lupus Erythematosus, Systemic - immunology Molecular immunology |
| title | Cross-reactivity of anti-DNA antibodies with proteoglycans |
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