Parasite antigens recognized by patients with cutaneous leishmaniasis
SUMMARY Humoral and cell‐mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple componen...
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| Veröffentlicht in: | Clinical and experimental immunology 1990-04, Vol.80 (1), p.77-82 |
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| creator | JAFFE, C. L. SHOR, R. TRAU, H. PASSWELL, J. H. |
| description | SUMMARY
Humoral and cell‐mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple components by Western blotting with molecular weights between 5000 and >200000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophos‐phoglycan. both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 μg/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen‐specific responses to a 72–82 kD component in promastigote lysates. |
| doi_str_mv | 10.1111/j.1365-2249.1990.tb06444.x |
| format | Article |
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Humoral and cell‐mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple components by Western blotting with molecular weights between 5000 and >200000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophos‐phoglycan. both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 μg/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen‐specific responses to a 72–82 kD component in promastigote lysates.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1111/j.1365-2249.1990.tb06444.x</identifier><identifier>PMID: 2323102</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies, Protozoan - immunology ; Antigens, Protozoan - immunology ; Blotting, Western ; Humans ; Leishmania major ; Leishmania tropica - immunology ; Leishmaniasis - immunology ; lipophosphoglycan ; Lymphocyte Activation - immunology ; lymphocyte proliferation ; parasite surface protease ; T-Lymphocytes - immunology</subject><ispartof>Clinical and experimental immunology, 1990-04, Vol.80 (1), p.77-82</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5067-41c6e51d951f1ce6c07f564f7d486ea44a4d528eb07de083fc80628de32233153</citedby><cites>FETCH-LOGICAL-c5067-41c6e51d951f1ce6c07f564f7d486ea44a4d528eb07de083fc80628de32233153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1535222/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1535222/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2323102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JAFFE, C. L.</creatorcontrib><creatorcontrib>SHOR, R.</creatorcontrib><creatorcontrib>TRAU, H.</creatorcontrib><creatorcontrib>PASSWELL, J. H.</creatorcontrib><title>Parasite antigens recognized by patients with cutaneous leishmaniasis</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>SUMMARY
Humoral and cell‐mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple components by Western blotting with molecular weights between 5000 and >200000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophos‐phoglycan. both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 μg/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen‐specific responses to a 72–82 kD component in promastigote lysates.</description><subject>Animals</subject><subject>Antibodies, Protozoan - immunology</subject><subject>Antigens, Protozoan - immunology</subject><subject>Blotting, Western</subject><subject>Humans</subject><subject>Leishmania major</subject><subject>Leishmania tropica - immunology</subject><subject>Leishmaniasis - immunology</subject><subject>lipophosphoglycan</subject><subject>Lymphocyte Activation - immunology</subject><subject>lymphocyte proliferation</subject><subject>parasite surface protease</subject><subject>T-Lymphocytes - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkV9LwzAUxYMoc04_glB88K01_5q0goKMqYOBPuhzSNPbLaNrZ9O5zU9vxsrQN83LJZx7DufyQ-iK4Ij4dzOPCBNxSClPI5KmOGozLDjn0eYI9Q_SMepjjNMwJZifojPn5v4rhKA91KOMMoJpH41edaOdbSHQVWunULmgAVNPK_sFeZBtg6VuLVStC9a2nQVm1eoK6pULSrButtCV9W53jk4KXTq46OYAvT-O3obP4eTlaTx8mIQmxkKGnBgBMcnTmBTEgDBYFrHghcx5IkBzrnke0wQyLHPACStMggVNcmCUMkZiNkD3-9zlKltAbnyxRpdq2diFbraq1lb9Vio7U9P6U3lvTH3IAF13AU39sQLXqoV1Bspyf5WSqcSS_WGR-N6E0dQv3u4XTVM710BxaEOw2tFSc7VDonZI1I6W6mipjTdf_rznYO3weP1ur69tCdt_JKvhaCwl-wZaaaXx</recordid><startdate>199004</startdate><enddate>199004</enddate><creator>JAFFE, C. L.</creator><creator>SHOR, R.</creator><creator>TRAU, H.</creator><creator>PASSWELL, J. H.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199004</creationdate><title>Parasite antigens recognized by patients with cutaneous leishmaniasis</title><author>JAFFE, C. L. ; SHOR, R. ; TRAU, H. ; PASSWELL, J. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5067-41c6e51d951f1ce6c07f564f7d486ea44a4d528eb07de083fc80628de32233153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Antibodies, Protozoan - immunology</topic><topic>Antigens, Protozoan - immunology</topic><topic>Blotting, Western</topic><topic>Humans</topic><topic>Leishmania major</topic><topic>Leishmania tropica - immunology</topic><topic>Leishmaniasis - immunology</topic><topic>lipophosphoglycan</topic><topic>Lymphocyte Activation - immunology</topic><topic>lymphocyte proliferation</topic><topic>parasite surface protease</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JAFFE, C. L.</creatorcontrib><creatorcontrib>SHOR, R.</creatorcontrib><creatorcontrib>TRAU, H.</creatorcontrib><creatorcontrib>PASSWELL, J. H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JAFFE, C. L.</au><au>SHOR, R.</au><au>TRAU, H.</au><au>PASSWELL, J. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parasite antigens recognized by patients with cutaneous leishmaniasis</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1990-04</date><risdate>1990</risdate><volume>80</volume><issue>1</issue><spage>77</spage><epage>82</epage><pages>77-82</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><abstract>SUMMARY
Humoral and cell‐mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple components by Western blotting with molecular weights between 5000 and >200000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophos‐phoglycan. both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 μg/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen‐specific responses to a 72–82 kD component in promastigote lysates.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2323102</pmid><doi>10.1111/j.1365-2249.1990.tb06444.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical and experimental immunology, 1990-04, Vol.80 (1), p.77-82 |
| issn | 0009-9104 1365-2249 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Protozoan - immunology Antigens, Protozoan - immunology Blotting, Western Humans Leishmania major Leishmania tropica - immunology Leishmaniasis - immunology lipophosphoglycan Lymphocyte Activation - immunology lymphocyte proliferation parasite surface protease T-Lymphocytes - immunology |
| title | Parasite antigens recognized by patients with cutaneous leishmaniasis |
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