Intraperitoneal immunization of human subjects with tetanus toxoid induces specific antibody‐secreting cells in the peritoneal cavity and in the circulation, but fails to elicit a secretory IgA response
SUMMARY Five patients on continuous ambulatory peritoneal dialysis (CAPD) were immunized intraperitoneally with tetanus toxoid (TT) through an indwelling catheter. Four control patients on CAPD received the same dose of TT intramuscularly. Before immunization, virtually no anti‐TT antibody‐secreting...
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| Veröffentlicht in: | Clinical and experimental immunology 1994-05, Vol.96 (2), p.356-363 |
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| creator | LUE, C. WALL BAKE, A. W. L. PRINCE, S. J. JULIAN, B. A. TSENG, M.‐L. RADL, J. ELSON, C. O. MESTECKY, J. |
| description | SUMMARY
Five patients on continuous ambulatory peritoneal dialysis (CAPD) were immunized intraperitoneally with tetanus toxoid (TT) through an indwelling catheter. Four control patients on CAPD received the same dose of TT intramuscularly. Before immunization, virtually no anti‐TT antibody‐secreting cells (AbSC) were detected by the enzyme‐linked immunospot (ELISPOT) assay in peripheral blood or peritoneal fluid from patients of either group. One to 2 weeks after immunization, high frequencies of TT‐specific AbSC were detected in the circulation and peritoneal cavity. More than 80% of those cells were of the IgG isotype, with IgA accounting for most of the remainder. Patients receiving TT by the i.p. route showed significantly higher frequencies of specific IgG and IgA AbSC in the peritoneal cavity than patients immunized intramuscularly. Frequencies of AbSC in peripheral blood did not significantly differ between the two groups. Immunization with TT by both routes resulted in a significant increase of IgG anti‐TT antibodies in serum, saliva and peritoneal fluid. A significant IgA antibody response was seen only in serum and peritoneal effluents. Therefore, i.p. immunization of human subjects with TT elicited both a localized response in the peritoneal cavity as well as a systemic response in serum, but did not induce a salivary IgA response. |
| doi_str_mv | 10.1111/j.1365-2249.1994.tb06567.x |
| format | Article |
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Five patients on continuous ambulatory peritoneal dialysis (CAPD) were immunized intraperitoneally with tetanus toxoid (TT) through an indwelling catheter. Four control patients on CAPD received the same dose of TT intramuscularly. Before immunization, virtually no anti‐TT antibody‐secreting cells (AbSC) were detected by the enzyme‐linked immunospot (ELISPOT) assay in peripheral blood or peritoneal fluid from patients of either group. One to 2 weeks after immunization, high frequencies of TT‐specific AbSC were detected in the circulation and peritoneal cavity. More than 80% of those cells were of the IgG isotype, with IgA accounting for most of the remainder. Patients receiving TT by the i.p. route showed significantly higher frequencies of specific IgG and IgA AbSC in the peritoneal cavity than patients immunized intramuscularly. Frequencies of AbSC in peripheral blood did not significantly differ between the two groups. Immunization with TT by both routes resulted in a significant increase of IgG anti‐TT antibodies in serum, saliva and peritoneal fluid. A significant IgA antibody response was seen only in serum and peritoneal effluents. Therefore, i.p. immunization of human subjects with TT elicited both a localized response in the peritoneal cavity as well as a systemic response in serum, but did not induce a salivary IgA response.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1111/j.1365-2249.1994.tb06567.x</identifier><identifier>PMID: 8187345</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; African Continental Ancestry Group ; Analysis of the immune response. Humoral and cellular immunity ; Antibodies - blood ; Antibody production ; Antibody-Producing Cells - immunology ; antibody‐secreting cells ; Ascitic Fluid - immunology ; Biological and medical sciences ; Clostridium tetani ; Cytoplasm - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; IgA ; Immunobiology ; Immunoglobulin A - analysis ; Immunoglobulin G - analysis ; Immunoglobulin Isotypes - analysis ; Infusions, Parenteral ; Male ; Middle Aged ; Peritoneal Dialysis, Continuous Ambulatory ; peritoneal immunization ; Peritoneum - cytology ; Peritoneum - immunology ; Phenotype ; Safety ; Saliva - immunology ; secretory immunity ; tetanus toxoid ; Tetanus Toxoid - immunology</subject><ispartof>Clinical and experimental immunology, 1994-05, Vol.96 (2), p.356-363</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4516-f8551ac7efc8c3dccf5e97b5cf735932bb3a256b01ff68da98533d19020cee3d3</citedby><cites>FETCH-LOGICAL-c4516-f8551ac7efc8c3dccf5e97b5cf735932bb3a256b01ff68da98533d19020cee3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1534882/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1534882/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4091138$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8187345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LUE, C.</creatorcontrib><creatorcontrib>WALL BAKE, A. W. L.</creatorcontrib><creatorcontrib>PRINCE, S. J.</creatorcontrib><creatorcontrib>JULIAN, B. A.</creatorcontrib><creatorcontrib>TSENG, M.‐L.</creatorcontrib><creatorcontrib>RADL, J.</creatorcontrib><creatorcontrib>ELSON, C. O.</creatorcontrib><creatorcontrib>MESTECKY, J.</creatorcontrib><title>Intraperitoneal immunization of human subjects with tetanus toxoid induces specific antibody‐secreting cells in the peritoneal cavity and in the circulation, but fails to elicit a secretory IgA response</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>SUMMARY
Five patients on continuous ambulatory peritoneal dialysis (CAPD) were immunized intraperitoneally with tetanus toxoid (TT) through an indwelling catheter. Four control patients on CAPD received the same dose of TT intramuscularly. Before immunization, virtually no anti‐TT antibody‐secreting cells (AbSC) were detected by the enzyme‐linked immunospot (ELISPOT) assay in peripheral blood or peritoneal fluid from patients of either group. One to 2 weeks after immunization, high frequencies of TT‐specific AbSC were detected in the circulation and peritoneal cavity. More than 80% of those cells were of the IgG isotype, with IgA accounting for most of the remainder. Patients receiving TT by the i.p. route showed significantly higher frequencies of specific IgG and IgA AbSC in the peritoneal cavity than patients immunized intramuscularly. Frequencies of AbSC in peripheral blood did not significantly differ between the two groups. Immunization with TT by both routes resulted in a significant increase of IgG anti‐TT antibodies in serum, saliva and peritoneal fluid. A significant IgA antibody response was seen only in serum and peritoneal effluents. Therefore, i.p. immunization of human subjects with TT elicited both a localized response in the peritoneal cavity as well as a systemic response in serum, but did not induce a salivary IgA response.</description><subject>Adult</subject><subject>African Continental Ancestry Group</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Antibodies - blood</subject><subject>Antibody production</subject><subject>Antibody-Producing Cells - immunology</subject><subject>antibody‐secreting cells</subject><subject>Ascitic Fluid - immunology</subject><subject>Biological and medical sciences</subject><subject>Clostridium tetani</subject><subject>Cytoplasm - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>IgA</subject><subject>Immunobiology</subject><subject>Immunoglobulin A - analysis</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin Isotypes - analysis</subject><subject>Infusions, Parenteral</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Peritoneal Dialysis, Continuous Ambulatory</subject><subject>peritoneal immunization</subject><subject>Peritoneum - cytology</subject><subject>Peritoneum - immunology</subject><subject>Phenotype</subject><subject>Safety</subject><subject>Saliva - immunology</subject><subject>secretory immunity</subject><subject>tetanus toxoid</subject><subject>Tetanus Toxoid - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUsGO0zAUjBBoWRY-AclCiBMtdhw7NgekVbVApZW4wNlynOfWVWIX29ltOfEJfBhfwZeQ0FKWE8IXy5p588ajKYpnBM_JeF5t5oRyNivLSs6JlNU8N5gzXs9394rzE3S_OMcYy5kkuHpYPEppMz455-VZcSaIqGnFzovvS5-j3kJ0OXjQHXJ9P3j3RWcXPAoWrYdee5SGZgMmJ3Tr8hplyNoPCeWwC65FzreDgYTSFoyzziDts2tCu__x9VsCEyE7v0IGui6NXJTXgO4sNPrG5f040_4GjYtm6H45eImaISOrXTdtQ9A54zLS6CAb4h4tV5coQtoGn-Bx8cDqLsGT431RfHp79XHxfnb94d1ycXk9MxUjfGYFY0SbGqwRhrbGWAaybpixNWWSlk1Ddcl4g4m1XLRaCkZpSyQusQGgLb0o3hx0t0PTQ2tgyrBT2-h6HfcqaKf-Rrxbq1W4UYTRSohyFHhxFIjh8wApq96lKSDtIQxJ1bwSdSXIP4mEy5oROim-PhBNDClFsCc3BKupNGqjpmaoqRlqKo06lkbtxuGnd_9zGj22ZMSfH3GdjO5s1N64dKJVWBJCxZ9Ybl0H-_8woBZXS8o4_QkNqufK</recordid><startdate>199405</startdate><enddate>199405</enddate><creator>LUE, C.</creator><creator>WALL BAKE, A. W. L.</creator><creator>PRINCE, S. J.</creator><creator>JULIAN, B. A.</creator><creator>TSENG, M.‐L.</creator><creator>RADL, J.</creator><creator>ELSON, C. O.</creator><creator>MESTECKY, J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199405</creationdate><title>Intraperitoneal immunization of human subjects with tetanus toxoid induces specific antibody‐secreting cells in the peritoneal cavity and in the circulation, but fails to elicit a secretory IgA response</title><author>LUE, C. ; WALL BAKE, A. W. L. ; PRINCE, S. J. ; JULIAN, B. A. ; TSENG, M.‐L. ; RADL, J. ; ELSON, C. O. ; MESTECKY, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4516-f8551ac7efc8c3dccf5e97b5cf735932bb3a256b01ff68da98533d19020cee3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adult</topic><topic>African Continental Ancestry Group</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Antibodies - blood</topic><topic>Antibody production</topic><topic>Antibody-Producing Cells - immunology</topic><topic>antibody‐secreting cells</topic><topic>Ascitic Fluid - immunology</topic><topic>Biological and medical sciences</topic><topic>Clostridium tetani</topic><topic>Cytoplasm - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>IgA</topic><topic>Immunobiology</topic><topic>Immunoglobulin A - analysis</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin Isotypes - analysis</topic><topic>Infusions, Parenteral</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Peritoneal Dialysis, Continuous Ambulatory</topic><topic>peritoneal immunization</topic><topic>Peritoneum - cytology</topic><topic>Peritoneum - immunology</topic><topic>Phenotype</topic><topic>Safety</topic><topic>Saliva - immunology</topic><topic>secretory immunity</topic><topic>tetanus toxoid</topic><topic>Tetanus Toxoid - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LUE, C.</creatorcontrib><creatorcontrib>WALL BAKE, A. W. L.</creatorcontrib><creatorcontrib>PRINCE, S. J.</creatorcontrib><creatorcontrib>JULIAN, B. A.</creatorcontrib><creatorcontrib>TSENG, M.‐L.</creatorcontrib><creatorcontrib>RADL, J.</creatorcontrib><creatorcontrib>ELSON, C. O.</creatorcontrib><creatorcontrib>MESTECKY, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LUE, C.</au><au>WALL BAKE, A. W. L.</au><au>PRINCE, S. J.</au><au>JULIAN, B. A.</au><au>TSENG, M.‐L.</au><au>RADL, J.</au><au>ELSON, C. O.</au><au>MESTECKY, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intraperitoneal immunization of human subjects with tetanus toxoid induces specific antibody‐secreting cells in the peritoneal cavity and in the circulation, but fails to elicit a secretory IgA response</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1994-05</date><risdate>1994</risdate><volume>96</volume><issue>2</issue><spage>356</spage><epage>363</epage><pages>356-363</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>SUMMARY
Five patients on continuous ambulatory peritoneal dialysis (CAPD) were immunized intraperitoneally with tetanus toxoid (TT) through an indwelling catheter. Four control patients on CAPD received the same dose of TT intramuscularly. Before immunization, virtually no anti‐TT antibody‐secreting cells (AbSC) were detected by the enzyme‐linked immunospot (ELISPOT) assay in peripheral blood or peritoneal fluid from patients of either group. One to 2 weeks after immunization, high frequencies of TT‐specific AbSC were detected in the circulation and peritoneal cavity. More than 80% of those cells were of the IgG isotype, with IgA accounting for most of the remainder. Patients receiving TT by the i.p. route showed significantly higher frequencies of specific IgG and IgA AbSC in the peritoneal cavity than patients immunized intramuscularly. Frequencies of AbSC in peripheral blood did not significantly differ between the two groups. Immunization with TT by both routes resulted in a significant increase of IgG anti‐TT antibodies in serum, saliva and peritoneal fluid. A significant IgA antibody response was seen only in serum and peritoneal effluents. Therefore, i.p. immunization of human subjects with TT elicited both a localized response in the peritoneal cavity as well as a systemic response in serum, but did not induce a salivary IgA response.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8187345</pmid><doi>10.1111/j.1365-2249.1994.tb06567.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult African Continental Ancestry Group Analysis of the immune response. Humoral and cellular immunity Antibodies - blood Antibody production Antibody-Producing Cells - immunology antibody‐secreting cells Ascitic Fluid - immunology Biological and medical sciences Clostridium tetani Cytoplasm - immunology Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans IgA Immunobiology Immunoglobulin A - analysis Immunoglobulin G - analysis Immunoglobulin Isotypes - analysis Infusions, Parenteral Male Middle Aged Peritoneal Dialysis, Continuous Ambulatory peritoneal immunization Peritoneum - cytology Peritoneum - immunology Phenotype Safety Saliva - immunology secretory immunity tetanus toxoid Tetanus Toxoid - immunology |
| title | Intraperitoneal immunization of human subjects with tetanus toxoid induces specific antibody‐secreting cells in the peritoneal cavity and in the circulation, but fails to elicit a secretory IgA response |
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