HoxB domain induction silences DNA replication origins in the locus and specifies a single origin at its boundary

In multicellular organisms, changes in the DNA replication programme could act to integrate differentiation with cell division in various developmental and transcriptional contexts. Here, we have addressed the use of DNA replication origins during differentiation in the HoxB domain—a cluster of nine...

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Veröffentlicht in:	EMBO reports 2006-08, Vol.7 (8), p.812-816
Hauptverfasser:	Grégoire, Damien, Brodolin, Konstantin, Méchali, Marcel
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Animals Cell Differentiation - drug effects Cell Differentiation - genetics Cell Differentiation - physiology Cell Line, Tumor Chromatin Immunoprecipitation Deoxyribonucleic acid DNA DNA Replication - genetics Gene expression Gene Expression Regulation - drug effects Gene Expression Regulation - genetics Gene loci Genetics Histones - genetics Histones - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Human genetics Life Sciences Models, Genetic Origin Recognition Complex - genetics Origin Recognition Complex - metabolism Prenatal development Replication Origin - genetics Rodents Scientific Report Scientific Reports Transcription, Genetic - drug effects Transcription, Genetic - genetics Tretinoin - pharmacology
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creator	Grégoire, Damien Brodolin, Konstantin Méchali, Marcel
description	In multicellular organisms, changes in the DNA replication programme could act to integrate differentiation with cell division in various developmental and transcriptional contexts. Here, we have addressed the use of DNA replication origins during differentiation in the HoxB domain—a cluster of nine genes developmentally regulated in a collinear manner. In undifferentiated mouse P19 cells, we detected several DNA replication origins in the 100 kb HoxB locus, indicating a relaxed origin use when the locus is transcriptionally silent. By contrast, in retinoic‐acid‐induced differentiated cells, when HoxB transcription is activated, a general silencing of DNA replication origins occurs in the locus except one located downstream of Hoxb1 , at the 3′ boundary of the HoxB domain. Silencing of the replication origins is associated with histone hyperacetylation, whereas the active Hoxb1 origin persists as a hypoacetylated island. These findings provide direct evidence for the differentiated use of origins in HoxB genes, and we suggest that this regulation might contribute to the regulated expression of HoxB genes during development.
doi_str_mv	10.1038/sj.embor.7400758
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These findings provide direct evidence for the differentiated use of origins in HoxB genes, and we suggest that this regulation might contribute to the regulated expression of HoxB genes during development.</description><subject>Acetylation</subject><subject>Animals</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line, Tumor</subject><subject>Chromatin Immunoprecipitation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Replication - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - genetics</subject><subject>Gene loci</subject><subject>Genetics</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Human genetics</subject><subject>Life Sciences</subject><subject>Models, Genetic</subject><subject>Origin Recognition Complex - genetics</subject><subject>Origin Recognition Complex - metabolism</subject><subject>Prenatal development</subject><subject>Replication Origin - genetics</subject><subject>Rodents</subject><subject>Scientific Report</subject><subject>Scientific Reports</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transcription, Genetic - genetics</subject><subject>Tretinoin - pharmacology</subject><issn>1469-221X</issn><issn>1469-3178</issn><issn>1469-221X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFks1v0zAYxiMEYmNw54QsDkgcWuw4_rogdZ8FlSIhEIiL5ThO65LYnZ2M7b_HXaIVdmAnW_bved5Hr54se4ngFEHM38XN1LSlD1NWQMgIf5QdooKKCUaMPx7veY5-HGTPYtxACIlg_Gl2gCgvCKb8MLuc--tjUPlWWQesq3rdWe9AtI1x2kRwupyBYLaN1er2wwe7si4mFHRrAxqv-wiUq0DcGm1rmyQqqd2qMSMLVAdsF0Hpe1epcPM8e1KrJpoX43mUfTs_-3oynyw-X3w4mS0mmvKcT1AJVS0wygXPkeEUGaQFLDEuCpIXuNQVEznGpBQE5VDpmhjCaEUULDBmrMZH2fvBd9uXram0cV1QjdwG26YU0isr__1xdi1X_koikhNEUDJ4Oxis78nms4XcvUGIEC5gcbVj34zDgr_sTexka6M2TaOc8X2UlFMhKH0YRIyKXIgiga_vgRvfB5c2JnPI0wogowmCA6SDjzGY-i4ngnLXEBk38rYhcmxIkrz6ey17wViJBIgB-J06cPOgoTz7dPxlb44GbUwytzJhH_o_gSaDxsbOXN_NU-GXpAwzIr8vL-Q5_LmApx-FXOI_firp9w</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>Grégoire, Damien</creator><creator>Brodolin, Konstantin</creator><creator>Méchali, Marcel</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><general>EMBO Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3124-7586</orcidid></search><sort><creationdate>200608</creationdate><title>HoxB domain induction silences DNA replication origins in the locus and specifies a single origin at its boundary</title><author>Grégoire, Damien ; Brodolin, Konstantin ; Méchali, Marcel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6828-1b0af93129821e861e1c90b33445243bcd792335b95120acf5e576d5a043377f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acetylation</topic><topic>Animals</topic><topic>Cell Differentiation - 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subjects	Acetylation Animals Cell Differentiation - drug effects Cell Differentiation - genetics Cell Differentiation - physiology Cell Line, Tumor Chromatin Immunoprecipitation Deoxyribonucleic acid DNA DNA Replication - genetics Gene expression Gene Expression Regulation - drug effects Gene Expression Regulation - genetics Gene loci Genetics Histones - genetics Histones - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Human genetics Life Sciences Models, Genetic Origin Recognition Complex - genetics Origin Recognition Complex - metabolism Prenatal development Replication Origin - genetics Rodents Scientific Report Scientific Reports Transcription, Genetic - drug effects Transcription, Genetic - genetics Tretinoin - pharmacology
title	HoxB domain induction silences DNA replication origins in the locus and specifies a single origin at its boundary
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