Effect of Mineral Particles Containing Iron on Primary Cultures of Rabbit Tracheal Epithelial Cells: Possible Implication of Oxidative Stress
Environmental mineral particles such as asbestos are responsible for numerous respiratory diseases. In addition to effects related to their geometry, particles are now assumed to act by triggering an oxidative stress process. Iron-containing particles, in particular, can produce oxygen-activated spe...
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| Veröffentlicht in: | Environmental health perspectives 1993-10, Vol.101 (5), p.436-442 |
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| description | Environmental mineral particles such as asbestos are responsible for numerous respiratory diseases. In addition to effects related to their geometry, particles are now assumed to act by triggering an oxidative stress process. Iron-containing particles, in particular, can produce oxygen-activated species by oxidizing their iron. To evaluate the involvement of iron-containing particles in respiratory diseases, three mineral particles (chrysotile, nemalite, and hematite) were tested in primary cultures of tracheal epithelium. Because of the ciliary beat, the three mineral particles were quickly concentrated at the periphery of the mucociliary epithelium, reconstituted in vitro where they induced cellular lesions. Endocytosis of the three types of particles was observed. Cytotoxicity studies have indicated that among the tested particles, the most cytostatic after 24 hr of treatment was the one that contained more Fe2+available on the surface, nemalite. Moreover, the effect of nemalite was reduced by pretreatment with desferrioxamine. As mineral particles, especially asbestos, are suspected to induce squamous metaplasia, we chose to study two specific transformations of the epithelium: the expression of cytokeratin-13 and the formation of cross-linked envelopes. Under our culture conditions, nemalite and chrysotile increased the expression of the cytokeratin-13, a specific marker of squamous metaplasia, whereas nemalite was the only particle able to strongly induce the formation of cross-linked envelopes. Nemalite was the most cytostatic particle and the most efficient at inducing squamous metaplasia. Measures of oxidizing power by electronspin resonance revealed that nemalite produced the most oxygen-activated species. This observation and its reduced toxicity by the desferrioxamine treatment suggest that nemalite could act on rabbit tracheal epithelial cells by an oxidative stress process. |
| doi_str_mv | 10.1289/ehp.93101436 |
| format | Article |
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In addition to effects related to their geometry, particles are now assumed to act by triggering an oxidative stress process. Iron-containing particles, in particular, can produce oxygen-activated species by oxidizing their iron. To evaluate the involvement of iron-containing particles in respiratory diseases, three mineral particles (chrysotile, nemalite, and hematite) were tested in primary cultures of tracheal epithelium. Because of the ciliary beat, the three mineral particles were quickly concentrated at the periphery of the mucociliary epithelium, reconstituted in vitro where they induced cellular lesions. Endocytosis of the three types of particles was observed. Cytotoxicity studies have indicated that among the tested particles, the most cytostatic after 24 hr of treatment was the one that contained more Fe2+available on the surface, nemalite. Moreover, the effect of nemalite was reduced by pretreatment with desferrioxamine. As mineral particles, especially asbestos, are suspected to induce squamous metaplasia, we chose to study two specific transformations of the epithelium: the expression of cytokeratin-13 and the formation of cross-linked envelopes. Under our culture conditions, nemalite and chrysotile increased the expression of the cytokeratin-13, a specific marker of squamous metaplasia, whereas nemalite was the only particle able to strongly induce the formation of cross-linked envelopes. Nemalite was the most cytostatic particle and the most efficient at inducing squamous metaplasia. Measures of oxidizing power by electronspin resonance revealed that nemalite produced the most oxygen-activated species. 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In addition to effects related to their geometry, particles are now assumed to act by triggering an oxidative stress process. Iron-containing particles, in particular, can produce oxygen-activated species by oxidizing their iron. To evaluate the involvement of iron-containing particles in respiratory diseases, three mineral particles (chrysotile, nemalite, and hematite) were tested in primary cultures of tracheal epithelium. Because of the ciliary beat, the three mineral particles were quickly concentrated at the periphery of the mucociliary epithelium, reconstituted in vitro where they induced cellular lesions. Endocytosis of the three types of particles was observed. Cytotoxicity studies have indicated that among the tested particles, the most cytostatic after 24 hr of treatment was the one that contained more Fe2+available on the surface, nemalite. Moreover, the effect of nemalite was reduced by pretreatment with desferrioxamine. As mineral particles, especially asbestos, are suspected to induce squamous metaplasia, we chose to study two specific transformations of the epithelium: the expression of cytokeratin-13 and the formation of cross-linked envelopes. Under our culture conditions, nemalite and chrysotile increased the expression of the cytokeratin-13, a specific marker of squamous metaplasia, whereas nemalite was the only particle able to strongly induce the formation of cross-linked envelopes. Nemalite was the most cytostatic particle and the most efficient at inducing squamous metaplasia. Measures of oxidizing power by electronspin resonance revealed that nemalite produced the most oxygen-activated species. This observation and its reduced toxicity by the desferrioxamine treatment suggest that nemalite could act on rabbit tracheal epithelial cells by an oxidative stress process.</description><subject>Animals</subject><subject>Asbestos</subject><subject>Asbestos - toxicity</subject><subject>Cell growth</subject><subject>Cell lines</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Epithelial Cells</subject><subject>Epithelium</subject><subject>Epithelium - drug effects</subject><subject>Ferric Compounds - toxicity</subject><subject>Free Radicals</subject><subject>Hematite</subject><subject>Iron - toxicity</subject><subject>Keratins</subject><subject>Metaplasia</subject><subject>Metaplasia - chemically induced</subject><subject>Minerals</subject><subject>Oxidation-Reduction</subject><subject>Rabbits</subject><subject>Stress, Physiological - chemically induced</subject><subject>Stress, Physiological - pathology</subject><subject>Trachea - cytology</subject><subject>Trachea - drug effects</subject><issn>0091-6765</issn><issn>1552-9924</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFvFCEYxYnR1LV682rCwXjqVBgGBjyYmMmqm9R0o_VMgPno0szObIFp7B_h_yybXZt6MiEB8h6_fI-H0GtKzmkt1XvY7M4Vo4Q2TDxBC8p5XSlVN0_RghBFK9EK_hy9SOmGEEKlECfoRFKqas4X6PfSe3AZTx5_CyNEM-C1iTm4ARLupjGbMIbxGq_iNOKy1jFsTbzH3TzkORZPefjdWBsyvorGbaAAlruQNzCEcuxgGNIHvJ5SCnYAvNruhuBMDnuax5e_Ql8ud4B_5AJLL9Ezb4YEr477Kfr5eXnVfa0uLr-suk8XlWOSi0rRljEDkgprlCOWS-4EBRDWAnhBPfXE1CVqL3ruWwq94YbUrXfSSmctO0UfD9zdbLfQOxhzSa53h3B6MkH_q4xho6-nO005VbIRBfDuCIjT7Qwp621IroQ1I0xz0lS0UqmW_N_YMMZqsTeeHYwuls-K4B-moUTve9alZ_2352J_8zjBg_lYbNHfHvSblKf4mFUz0mrWMKpIzf4AgK2ywA</recordid><startdate>19931001</startdate><enddate>19931001</enddate><creator>Guilianelli, Catherine</creator><creator>Baeza-Squiban, Armelle</creator><creator>Boisvieux-Ulrich, Emmanuelle</creator><creator>Houcine, Odile</creator><creator>Zalma, Roger</creator><creator>Guennou, Christiane</creator><creator>Pezerat, Henri</creator><creator>Marano, Francelyne</creator><general>National Institute of Environmental Health Sciences. 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In addition to effects related to their geometry, particles are now assumed to act by triggering an oxidative stress process. Iron-containing particles, in particular, can produce oxygen-activated species by oxidizing their iron. To evaluate the involvement of iron-containing particles in respiratory diseases, three mineral particles (chrysotile, nemalite, and hematite) were tested in primary cultures of tracheal epithelium. Because of the ciliary beat, the three mineral particles were quickly concentrated at the periphery of the mucociliary epithelium, reconstituted in vitro where they induced cellular lesions. Endocytosis of the three types of particles was observed. Cytotoxicity studies have indicated that among the tested particles, the most cytostatic after 24 hr of treatment was the one that contained more Fe2+available on the surface, nemalite. Moreover, the effect of nemalite was reduced by pretreatment with desferrioxamine. As mineral particles, especially asbestos, are suspected to induce squamous metaplasia, we chose to study two specific transformations of the epithelium: the expression of cytokeratin-13 and the formation of cross-linked envelopes. Under our culture conditions, nemalite and chrysotile increased the expression of the cytokeratin-13, a specific marker of squamous metaplasia, whereas nemalite was the only particle able to strongly induce the formation of cross-linked envelopes. Nemalite was the most cytostatic particle and the most efficient at inducing squamous metaplasia. Measures of oxidizing power by electronspin resonance revealed that nemalite produced the most oxygen-activated species. This observation and its reduced toxicity by the desferrioxamine treatment suggest that nemalite could act on rabbit tracheal epithelial cells by an oxidative stress process.</abstract><cop>United States</cop><pub>National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare</pub><pmid>8119255</pmid><doi>10.1289/ehp.93101436</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Animals Asbestos Asbestos - toxicity Cell growth Cell lines Cells, Cultured Cultured cells Electron Spin Resonance Spectroscopy Epithelial Cells Epithelium Epithelium - drug effects Ferric Compounds - toxicity Free Radicals Hematite Iron - toxicity Keratins Metaplasia Metaplasia - chemically induced Minerals Oxidation-Reduction Rabbits Stress, Physiological - chemically induced Stress, Physiological - pathology Trachea - cytology Trachea - drug effects |
| title | Effect of Mineral Particles Containing Iron on Primary Cultures of Rabbit Tracheal Epithelial Cells: Possible Implication of Oxidative Stress |
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