Analysis of Binding at a Single Spatially Localized Cluster of Binding Sites by Fluorescence Recovery after Photobleaching

Cells contain many subcellular structures in which specialized proteins locally cluster. Binding interactions within such clusters may be analyzed in live cells using models for fluorescence recovery after photobleaching (FRAP). Here we analyze a three-dimensional FRAP model that accounts for a sing...

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Veröffentlicht in:Biophysical journal 2006-08, Vol.91 (4), p.1169-1191
Hauptverfasser: Sprague, Brian L., Müller, Florian, Pego, Robert L., Bungay, Peter M., Stavreva, Diana A., McNally, James G.
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Sprache:eng
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