Singlet Oxygen Generation by UVA Light Exposure of Endogenous Photosensitizers
UVA light (320–400 nm) has been shown to produce deleterious biological effects in tissue due to the generation of singlet oxygen by substances like flavins or urocanic acid. Riboflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), β-nicotinamide adenine dinucleotide (NAD), and β-...
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| Veröffentlicht in: | Biophysical journal 2006-08, Vol.91 (4), p.1452-1459 |
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| creator | Baier, Jürgen Maisch, Tim Maier, Max Engel, Eva Landthaler, Michael Bäumler, Wolfgang |
| description | UVA light (320–400
nm) has been shown to produce deleterious biological effects in tissue due to the generation of singlet oxygen by substances like flavins or urocanic acid. Riboflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD),
β-nicotinamide adenine dinucleotide (NAD), and
β-nicotinamide adenine dinucleotide phosphate (NADP), urocanic acid, or cholesterol in solution were excited at 355
nm. Singlet oxygen was directly detected by time-resolved measurement of its luminescence at 1270
nm. NAD, NADP, and cholesterol showed no luminescence signal possibly due to the very low absorption coefficient at 355
nm. Singlet oxygen luminescence of urocanic acid was clearly detected but the signal was too weak to quantify a quantum yield. The quantum yield of singlet oxygen was precisely determined for riboflavin (Φ
Δ
=
0.54
±
0.07), FMN (Φ
Δ
=
0.51
±
0.07), and FAD (Φ
Δ
=
0.07
±
0.02). In aerated solution, riboflavin and FMN generate more singlet oxygen than exogenous photosensitizers such as Photofrin, which are applied in photodynamic therapy to kill cancer cells. With decreasing oxygen concentration, the quantum yield of singlet oxygen generation decreased, which must be considered when assessing the role of singlet oxygen at low oxygen concentrations (inside tissue). |
| doi_str_mv | 10.1529/biophysj.106.082388 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1518628</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006349506718562</els_id><sourcerecordid>1095142801</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-9abd29d43306c5651171fae4fd9f2b9fa7c98df18a2e17f4ba2e96c0cd1f72623</originalsourceid><addsrcrecordid>eNp9kU9rGzEQxUVpaNy0n6BQRO_rStqVVjq0EIKTFkwTaNOr2JVGtowjuZI2xP30VbD775LTwMxv3jzmIfSGkjnlTL0ffdyt93kzp0TMiWStlM_QjPKONYRI8RzNCCGiaTvFT9HLnDeEUMYJfYFOqeg5ZW03Q1---rDaQsHXD_sVBHwFAdJQfAx43OPb7-d46VfrghcPu5inBDg6vAg2VjZOGd-sY4kZQvbF_4SUX6ETN2wzvD7WM3R7ufh28alZXl99vjhfNqaTXWnUMFqmbNe2RBguOKU9dQN0zirHRuWG3ihpHZUDA9q7bqxVCUOMpa5ngrVn6ONBdzeNd2ANhJKGrd4lfzekvY6D1_9Pgl_rVbzXlFMpmKwC744CKf6YIBe9iVMK1bNmlPekb4mqUHuATIo5J3B_DlCiHzPQvzOoDaEPGdStt_96-7tzfHoFPhwAqB-695B0Nh6CAesTmKJt9E8e-AUXapv9</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>215707309</pqid></control><display><type>article</type><title>Singlet Oxygen Generation by UVA Light Exposure of Endogenous Photosensitizers</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Cell Press Free Archives</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Baier, Jürgen ; Maisch, Tim ; Maier, Max ; Engel, Eva ; Landthaler, Michael ; Bäumler, Wolfgang</creator><creatorcontrib>Baier, Jürgen ; Maisch, Tim ; Maier, Max ; Engel, Eva ; Landthaler, Michael ; Bäumler, Wolfgang</creatorcontrib><description>UVA light (320–400
nm) has been shown to produce deleterious biological effects in tissue due to the generation of singlet oxygen by substances like flavins or urocanic acid. Riboflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD),
β-nicotinamide adenine dinucleotide (NAD), and
β-nicotinamide adenine dinucleotide phosphate (NADP), urocanic acid, or cholesterol in solution were excited at 355
nm. Singlet oxygen was directly detected by time-resolved measurement of its luminescence at 1270
nm. NAD, NADP, and cholesterol showed no luminescence signal possibly due to the very low absorption coefficient at 355
nm. Singlet oxygen luminescence of urocanic acid was clearly detected but the signal was too weak to quantify a quantum yield. The quantum yield of singlet oxygen was precisely determined for riboflavin (Φ
Δ
=
0.54
±
0.07), FMN (Φ
Δ
=
0.51
±
0.07), and FAD (Φ
Δ
=
0.07
±
0.02). In aerated solution, riboflavin and FMN generate more singlet oxygen than exogenous photosensitizers such as Photofrin, which are applied in photodynamic therapy to kill cancer cells. With decreasing oxygen concentration, the quantum yield of singlet oxygen generation decreased, which must be considered when assessing the role of singlet oxygen at low oxygen concentrations (inside tissue).</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1529/biophysj.106.082388</identifier><identifier>PMID: 16751234</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Biological Factors - chemistry ; Biological Factors - radiation effects ; Dose-Response Relationship, Radiation ; Environmental Exposure ; Flavins - chemistry ; Flavins - radiation effects ; Luminescence ; Nucleic Acids ; Oxygen ; Oxygen - chemistry ; Photosensitizing Agents - chemistry ; Photosensitizing Agents - radiation effects ; Photosynthesis ; Radiation Dosage ; Tissues ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>Biophysical journal, 2006-08, Vol.91 (4), p.1452-1459</ispartof><rights>2006 The Biophysical Society</rights><rights>Copyright Biophysical Society Aug 15, 2006</rights><rights>Copyright © 2006, Biophysical Society 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-9abd29d43306c5651171fae4fd9f2b9fa7c98df18a2e17f4ba2e96c0cd1f72623</citedby><cites>FETCH-LOGICAL-c484t-9abd29d43306c5651171fae4fd9f2b9fa7c98df18a2e17f4ba2e96c0cd1f72623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1518628/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1529/biophysj.106.082388$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3550,27924,27925,45995,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16751234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baier, Jürgen</creatorcontrib><creatorcontrib>Maisch, Tim</creatorcontrib><creatorcontrib>Maier, Max</creatorcontrib><creatorcontrib>Engel, Eva</creatorcontrib><creatorcontrib>Landthaler, Michael</creatorcontrib><creatorcontrib>Bäumler, Wolfgang</creatorcontrib><title>Singlet Oxygen Generation by UVA Light Exposure of Endogenous Photosensitizers</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>UVA light (320–400
nm) has been shown to produce deleterious biological effects in tissue due to the generation of singlet oxygen by substances like flavins or urocanic acid. Riboflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD),
β-nicotinamide adenine dinucleotide (NAD), and
β-nicotinamide adenine dinucleotide phosphate (NADP), urocanic acid, or cholesterol in solution were excited at 355
nm. Singlet oxygen was directly detected by time-resolved measurement of its luminescence at 1270
nm. NAD, NADP, and cholesterol showed no luminescence signal possibly due to the very low absorption coefficient at 355
nm. Singlet oxygen luminescence of urocanic acid was clearly detected but the signal was too weak to quantify a quantum yield. The quantum yield of singlet oxygen was precisely determined for riboflavin (Φ
Δ
=
0.54
±
0.07), FMN (Φ
Δ
=
0.51
±
0.07), and FAD (Φ
Δ
=
0.07
±
0.02). In aerated solution, riboflavin and FMN generate more singlet oxygen than exogenous photosensitizers such as Photofrin, which are applied in photodynamic therapy to kill cancer cells. With decreasing oxygen concentration, the quantum yield of singlet oxygen generation decreased, which must be considered when assessing the role of singlet oxygen at low oxygen concentrations (inside tissue).</description><subject>Biological Factors - chemistry</subject><subject>Biological Factors - radiation effects</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Environmental Exposure</subject><subject>Flavins - chemistry</subject><subject>Flavins - radiation effects</subject><subject>Luminescence</subject><subject>Nucleic Acids</subject><subject>Oxygen</subject><subject>Oxygen - chemistry</subject><subject>Photosensitizing Agents - chemistry</subject><subject>Photosensitizing Agents - radiation effects</subject><subject>Photosynthesis</subject><subject>Radiation Dosage</subject><subject>Tissues</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet 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Oxygen Generation by UVA Light Exposure of Endogenous Photosensitizers</title><author>Baier, Jürgen ; Maisch, Tim ; Maier, Max ; Engel, Eva ; Landthaler, Michael ; Bäumler, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-9abd29d43306c5651171fae4fd9f2b9fa7c98df18a2e17f4ba2e96c0cd1f72623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological Factors - chemistry</topic><topic>Biological Factors - radiation effects</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Environmental Exposure</topic><topic>Flavins - chemistry</topic><topic>Flavins - radiation effects</topic><topic>Luminescence</topic><topic>Nucleic Acids</topic><topic>Oxygen</topic><topic>Oxygen - chemistry</topic><topic>Photosensitizing Agents - chemistry</topic><topic>Photosensitizing Agents - radiation effects</topic><topic>Photosynthesis</topic><topic>Radiation Dosage</topic><topic>Tissues</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baier, Jürgen</creatorcontrib><creatorcontrib>Maisch, Tim</creatorcontrib><creatorcontrib>Maier, Max</creatorcontrib><creatorcontrib>Engel, Eva</creatorcontrib><creatorcontrib>Landthaler, Michael</creatorcontrib><creatorcontrib>Bäumler, Wolfgang</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences 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Basic</collection><collection>SIRS Editorial</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baier, Jürgen</au><au>Maisch, Tim</au><au>Maier, Max</au><au>Engel, Eva</au><au>Landthaler, Michael</au><au>Bäumler, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Singlet Oxygen Generation by UVA Light Exposure of Endogenous Photosensitizers</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>2006-08-15</date><risdate>2006</risdate><volume>91</volume><issue>4</issue><spage>1452</spage><epage>1459</epage><pages>1452-1459</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>UVA light (320–400
nm) has been shown to produce deleterious biological effects in tissue due to the generation of singlet oxygen by substances like flavins or urocanic acid. Riboflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD),
β-nicotinamide adenine dinucleotide (NAD), and
β-nicotinamide adenine dinucleotide phosphate (NADP), urocanic acid, or cholesterol in solution were excited at 355
nm. Singlet oxygen was directly detected by time-resolved measurement of its luminescence at 1270
nm. NAD, NADP, and cholesterol showed no luminescence signal possibly due to the very low absorption coefficient at 355
nm. Singlet oxygen luminescence of urocanic acid was clearly detected but the signal was too weak to quantify a quantum yield. The quantum yield of singlet oxygen was precisely determined for riboflavin (Φ
Δ
=
0.54
±
0.07), FMN (Φ
Δ
=
0.51
±
0.07), and FAD (Φ
Δ
=
0.07
±
0.02). In aerated solution, riboflavin and FMN generate more singlet oxygen than exogenous photosensitizers such as Photofrin, which are applied in photodynamic therapy to kill cancer cells. With decreasing oxygen concentration, the quantum yield of singlet oxygen generation decreased, which must be considered when assessing the role of singlet oxygen at low oxygen concentrations (inside tissue).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16751234</pmid><doi>10.1529/biophysj.106.082388</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biophysical journal, 2006-08, Vol.91 (4), p.1452-1459 |
| issn | 0006-3495 1542-0086 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1518628 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biological Factors - chemistry Biological Factors - radiation effects Dose-Response Relationship, Radiation Environmental Exposure Flavins - chemistry Flavins - radiation effects Luminescence Nucleic Acids Oxygen Oxygen - chemistry Photosensitizing Agents - chemistry Photosensitizing Agents - radiation effects Photosynthesis Radiation Dosage Tissues Ultraviolet radiation Ultraviolet Rays |
| title | Singlet Oxygen Generation by UVA Light Exposure of Endogenous Photosensitizers |
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