Delayed‐type hypersensitivity‐induced increase in vascular permeability in the mouse small intestine: inhibition by depletion of sensory neuropeptides and NK1 receptor blockade
1 This study investigates the effects of capsaicin‐induced depletion of sensory neuropeptides and of neurokinin! (NK1) receptor blockade on delayed‐type hypersensitivity (DTH)‐induced changes of vascular permeability in the small intestine of the mouse. 2 The DTH reaction in the small intestine was...
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| Veröffentlicht in: | British journal of pharmacology 1995-04, Vol.114 (7), p.1483-1489 |
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| creator | Kraneveld, A.D. Buckley, T.L. Heuven‐Nolsen, D. Schaik, Y. Koster, A. Sj Nijkamp, F.P. |
| description | 1 This study investigates the effects of capsaicin‐induced depletion of sensory neuropeptides and of neurokinin! (NK1) receptor blockade on delayed‐type hypersensitivity (DTH)‐induced changes of vascular permeability in the small intestine of the mouse.
2 The DTH reaction in the small intestine was elicited by dinitrofluorobenzene (DNFB)‐contact sensitization followed by oral dinitrobenzene sulphonic acid (DNBS) challenge. To assess vascular leakage the accumulation of the plasma marker, Evans blue (EB), was measured 2, 24 and 48 h after the challenge.
3 The small intestinal DTH reaction was characterized by a significant increase in vascular permeability 24 h after the challenge of previously sensitized mice when compared to vehicle‐sensitized mice (P |
| doi_str_mv | 10.1111/j.1476-5381.1995.tb13374.x |
| format | Article |
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2 The DTH reaction in the small intestine was elicited by dinitrofluorobenzene (DNFB)‐contact sensitization followed by oral dinitrobenzene sulphonic acid (DNBS) challenge. To assess vascular leakage the accumulation of the plasma marker, Evans blue (EB), was measured 2, 24 and 48 h after the challenge.
3 The small intestinal DTH reaction was characterized by a significant increase in vascular permeability 24 h after the challenge of previously sensitized mice when compared to vehicle‐sensitized mice (P<0.05, ANOVA). Capsaicin‐induced depletion of sensory neuropeptides, two weeks before the sensitization, completely inhibited the DTH‐induced increase in small intestinal vascular permeability at 24 h (P<0.05, ANOVA). Vehicle/control: 108.2 ± 8.6 ngEB mg−1 dry weight; vehicle/DTH 207.8 ±25.1 ngEB mg−l dry weight; capsaicin/control: 65.8 ± 11.9 ng EB mg−1 dry weight; capsaicin/ DTH: 84.3 ± 7.6 ng EB mg−1 dry weight.
4 The tachykinins, substance P and neurokinin A (1.5 to 50 × 10−11 mol per mouse, i.v.), induced an increase in vascular leakage in the small intestine of naive mice. The specific NK1 receptor antagonist, RP67580 (10−9 mol per mouse, i.v.) was the most effective in reducing the substance P‐induced plasma extravasation when compared with other NK receptor antagonists, FK224 and FK888.
5 Treatment of DNFB‐sensitized mice with RP67580 (10−9 mol per mouse, i.v.) immediately before and 1 h after the DNBS challenge resulted in a significant reduction of the DTH‐induced increase in vascular permeability at 24h (vehicle/control: 107.5 ±8.8 ng EB mg−1 dry weight; RP67580/control: 95.4±5.4ng EB mg−1 dry weight; vehicle/DTH: 206.6± 22.6ng EB mg−1 dry weight; RP67580/DTH: 132.6±13.6 ng EB mg−1 dry weight, P<0.05, ANOVA).
6 These results suggest that sensory nerves are involved in the development of small intestinal DTH reactions in the mouse. NK1 receptors could play an important role in the initiation of the DTH‐induced changes in vascular leakage.</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1111/j.1476-5381.1995.tb13374.x</identifier><identifier>PMID: 7606352</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Capillary Permeability - drug effects ; capsaicin ; Capsaicin - pharmacology ; delayed‐type hypersensitivity ; Dipeptides - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hypersensitivity, Delayed - immunology ; Indoles - pharmacology ; Intestine, Small - drug effects ; Intestine. Mesentery ; Isoindoles ; Male ; Mice ; Mice, Inbred BALB C ; mouse small intestine ; Neurokinin-1 Receptor Antagonists ; Neuropeptides - pharmacology ; Peptides, Cyclic - pharmacology ; sensory neuropeptides ; Small intestinal vascular permeability ; tachykinins ; Vertebrates: digestive system</subject><ispartof>British journal of pharmacology, 1995-04, Vol.114 (7), p.1483-1489</ispartof><rights>1995 British Pharmacological Society</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1510295/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1510295/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3483219$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7606352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kraneveld, A.D.</creatorcontrib><creatorcontrib>Buckley, T.L.</creatorcontrib><creatorcontrib>Heuven‐Nolsen, D.</creatorcontrib><creatorcontrib>Schaik, Y.</creatorcontrib><creatorcontrib>Koster, A. Sj</creatorcontrib><creatorcontrib>Nijkamp, F.P.</creatorcontrib><title>Delayed‐type hypersensitivity‐induced increase in vascular permeability in the mouse small intestine: inhibition by depletion of sensory neuropeptides and NK1 receptor blockade</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>1 This study investigates the effects of capsaicin‐induced depletion of sensory neuropeptides and of neurokinin! (NK1) receptor blockade on delayed‐type hypersensitivity (DTH)‐induced changes of vascular permeability in the small intestine of the mouse.
2 The DTH reaction in the small intestine was elicited by dinitrofluorobenzene (DNFB)‐contact sensitization followed by oral dinitrobenzene sulphonic acid (DNBS) challenge. To assess vascular leakage the accumulation of the plasma marker, Evans blue (EB), was measured 2, 24 and 48 h after the challenge.
3 The small intestinal DTH reaction was characterized by a significant increase in vascular permeability 24 h after the challenge of previously sensitized mice when compared to vehicle‐sensitized mice (P<0.05, ANOVA). Capsaicin‐induced depletion of sensory neuropeptides, two weeks before the sensitization, completely inhibited the DTH‐induced increase in small intestinal vascular permeability at 24 h (P<0.05, ANOVA). Vehicle/control: 108.2 ± 8.6 ngEB mg−1 dry weight; vehicle/DTH 207.8 ±25.1 ngEB mg−l dry weight; capsaicin/control: 65.8 ± 11.9 ng EB mg−1 dry weight; capsaicin/ DTH: 84.3 ± 7.6 ng EB mg−1 dry weight.
4 The tachykinins, substance P and neurokinin A (1.5 to 50 × 10−11 mol per mouse, i.v.), induced an increase in vascular leakage in the small intestine of naive mice. The specific NK1 receptor antagonist, RP67580 (10−9 mol per mouse, i.v.) was the most effective in reducing the substance P‐induced plasma extravasation when compared with other NK receptor antagonists, FK224 and FK888.
5 Treatment of DNFB‐sensitized mice with RP67580 (10−9 mol per mouse, i.v.) immediately before and 1 h after the DNBS challenge resulted in a significant reduction of the DTH‐induced increase in vascular permeability at 24h (vehicle/control: 107.5 ±8.8 ng EB mg−1 dry weight; RP67580/control: 95.4±5.4ng EB mg−1 dry weight; vehicle/DTH: 206.6± 22.6ng EB mg−1 dry weight; RP67580/DTH: 132.6±13.6 ng EB mg−1 dry weight, P<0.05, ANOVA).
6 These results suggest that sensory nerves are involved in the development of small intestinal DTH reactions in the mouse. NK1 receptors could play an important role in the initiation of the DTH‐induced changes in vascular leakage.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Capillary Permeability - drug effects</subject><subject>capsaicin</subject><subject>Capsaicin - pharmacology</subject><subject>delayed‐type hypersensitivity</subject><subject>Dipeptides - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypersensitivity, Delayed - immunology</subject><subject>Indoles - pharmacology</subject><subject>Intestine, Small - drug effects</subject><subject>Intestine. Mesentery</subject><subject>Isoindoles</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>mouse small intestine</subject><subject>Neurokinin-1 Receptor Antagonists</subject><subject>Neuropeptides - pharmacology</subject><subject>Peptides, Cyclic - pharmacology</subject><subject>sensory neuropeptides</subject><subject>Small intestinal vascular permeability</subject><subject>tachykinins</subject><subject>Vertebrates: digestive system</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUkuO1DAUtBBoaAaOgGQhxC7BjpM4ZoEGhs8gRsAC1pZjv6bdOE6wk2ay4wgchhNxEpyZqAVe2O-9KtWTyoXQI0pyms7TfU5LXmcVa2hOhajysaWM8TK_uoU2R-g22hBCeEZp09xF92LcE5JAXp2gE16TmlXFBv1-BU7NYP78_DXOA-BdukIEH-1oD3ac09x6M2kw2HodQEVIBT6oqCenAk7sDlRrXeIuwLgD3PVTYsVOOZdGI8TReniWyp1tk2zvcTtjA4OD66bf4mVhH2bsYQr9AMNoDUSsvMEf3lMcQKdRH3Drev1NGbiP7myVi_BgfU_RlzevP59fZJcf3747f3GZDUyIMitAc14XRAnBiBC6osWWGMJpW4m6YUo3rBVaFU1ZFVpvCW21qJkAylXyRhl2ip7f6A5T24HR4MegnByC7VSYZa-s_B_xdie_9gdJK0oKUSWBJ6tA6L9PyQjZ2ajBOeUhmSQ5Zw0jlCbiw383HVes_5TwxyuejFduG5TXNh5prGxYQUWind3QflgH8xGmRC65kXu5hEMu4ZBLbuSaG3klX366uC7ZX0Srv7c</recordid><startdate>199504</startdate><enddate>199504</enddate><creator>Kraneveld, A.D.</creator><creator>Buckley, T.L.</creator><creator>Heuven‐Nolsen, D.</creator><creator>Schaik, Y.</creator><creator>Koster, A. Sj</creator><creator>Nijkamp, F.P.</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199504</creationdate><title>Delayed‐type hypersensitivity‐induced increase in vascular permeability in the mouse small intestine: inhibition by depletion of sensory neuropeptides and NK1 receptor blockade</title><author>Kraneveld, A.D. ; Buckley, T.L. ; Heuven‐Nolsen, D. ; Schaik, Y. ; Koster, A. Sj ; Nijkamp, F.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p3994-2ec77620a993099c512f0d071b59683ac83b9ca28452ccf01bc9639e17a352ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Capillary Permeability - drug effects</topic><topic>capsaicin</topic><topic>Capsaicin - pharmacology</topic><topic>delayed‐type hypersensitivity</topic><topic>Dipeptides - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypersensitivity, Delayed - immunology</topic><topic>Indoles - pharmacology</topic><topic>Intestine, Small - drug effects</topic><topic>Intestine. Mesentery</topic><topic>Isoindoles</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>mouse small intestine</topic><topic>Neurokinin-1 Receptor Antagonists</topic><topic>Neuropeptides - pharmacology</topic><topic>Peptides, Cyclic - pharmacology</topic><topic>sensory neuropeptides</topic><topic>Small intestinal vascular permeability</topic><topic>tachykinins</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kraneveld, A.D.</creatorcontrib><creatorcontrib>Buckley, T.L.</creatorcontrib><creatorcontrib>Heuven‐Nolsen, D.</creatorcontrib><creatorcontrib>Schaik, Y.</creatorcontrib><creatorcontrib>Koster, A. Sj</creatorcontrib><creatorcontrib>Nijkamp, F.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kraneveld, A.D.</au><au>Buckley, T.L.</au><au>Heuven‐Nolsen, D.</au><au>Schaik, Y.</au><au>Koster, A. Sj</au><au>Nijkamp, F.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Delayed‐type hypersensitivity‐induced increase in vascular permeability in the mouse small intestine: inhibition by depletion of sensory neuropeptides and NK1 receptor blockade</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>1995-04</date><risdate>1995</risdate><volume>114</volume><issue>7</issue><spage>1483</spage><epage>1489</epage><pages>1483-1489</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>1 This study investigates the effects of capsaicin‐induced depletion of sensory neuropeptides and of neurokinin! (NK1) receptor blockade on delayed‐type hypersensitivity (DTH)‐induced changes of vascular permeability in the small intestine of the mouse.
2 The DTH reaction in the small intestine was elicited by dinitrofluorobenzene (DNFB)‐contact sensitization followed by oral dinitrobenzene sulphonic acid (DNBS) challenge. To assess vascular leakage the accumulation of the plasma marker, Evans blue (EB), was measured 2, 24 and 48 h after the challenge.
3 The small intestinal DTH reaction was characterized by a significant increase in vascular permeability 24 h after the challenge of previously sensitized mice when compared to vehicle‐sensitized mice (P<0.05, ANOVA). Capsaicin‐induced depletion of sensory neuropeptides, two weeks before the sensitization, completely inhibited the DTH‐induced increase in small intestinal vascular permeability at 24 h (P<0.05, ANOVA). Vehicle/control: 108.2 ± 8.6 ngEB mg−1 dry weight; vehicle/DTH 207.8 ±25.1 ngEB mg−l dry weight; capsaicin/control: 65.8 ± 11.9 ng EB mg−1 dry weight; capsaicin/ DTH: 84.3 ± 7.6 ng EB mg−1 dry weight.
4 The tachykinins, substance P and neurokinin A (1.5 to 50 × 10−11 mol per mouse, i.v.), induced an increase in vascular leakage in the small intestine of naive mice. The specific NK1 receptor antagonist, RP67580 (10−9 mol per mouse, i.v.) was the most effective in reducing the substance P‐induced plasma extravasation when compared with other NK receptor antagonists, FK224 and FK888.
5 Treatment of DNFB‐sensitized mice with RP67580 (10−9 mol per mouse, i.v.) immediately before and 1 h after the DNBS challenge resulted in a significant reduction of the DTH‐induced increase in vascular permeability at 24h (vehicle/control: 107.5 ±8.8 ng EB mg−1 dry weight; RP67580/control: 95.4±5.4ng EB mg−1 dry weight; vehicle/DTH: 206.6± 22.6ng EB mg−1 dry weight; RP67580/DTH: 132.6±13.6 ng EB mg−1 dry weight, P<0.05, ANOVA).
6 These results suggest that sensory nerves are involved in the development of small intestinal DTH reactions in the mouse. NK1 receptors could play an important role in the initiation of the DTH‐induced changes in vascular leakage.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7606352</pmid><doi>10.1111/j.1476-5381.1995.tb13374.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Capillary Permeability - drug effects capsaicin Capsaicin - pharmacology delayed‐type hypersensitivity Dipeptides - pharmacology Fundamental and applied biological sciences. Psychology Hypersensitivity, Delayed - immunology Indoles - pharmacology Intestine, Small - drug effects Intestine. Mesentery Isoindoles Male Mice Mice, Inbred BALB C mouse small intestine Neurokinin-1 Receptor Antagonists Neuropeptides - pharmacology Peptides, Cyclic - pharmacology sensory neuropeptides Small intestinal vascular permeability tachykinins Vertebrates: digestive system |
| title | Delayed‐type hypersensitivity‐induced increase in vascular permeability in the mouse small intestine: inhibition by depletion of sensory neuropeptides and NK1 receptor blockade |
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