Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma

The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Annals of surgery 1988-04, Vol.207 (4), p.455-461
Hauptverfasser: KHEIR, S. M, BINES, S. D, VONROENN, J. H, SENG-JAW SOONG, URIST, M. M, COON, J. S
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 461
container_issue 4
container_start_page 455
container_title Annals of surgery
container_volume 207
creator KHEIR, S. M
BINES, S. D
VONROENN, J. H
SENG-JAW SOONG
URIST, M. M
COON, J. S
description The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma.
doi_str_mv 10.1097/00000658-198804000-00014
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1493423</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78153469</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3594-fb8a7d856b339aa849ec421733d262f8ae9363a228ef6226947e80ed3ffadc393</originalsourceid><addsrcrecordid>eNpVUcFO3DAQtVARXSifUMkH1FvA9jiOfamEoKVICDjQszXr2FujJF7ipBJ_X293WYEly5p5M2_G7xFCOTvnzDQXbHNUrStutGayBFW5XB6QBa9FSXPJPpFFyUElDYjP5Djn502FZs0ROQKoa6HMgtw8jmk1pDxFR3NcDTFEh4PzNAV6fX9JcfDzukuxfaVxoHnClae31M1TAdKcae87HFKPX8hhwC770917Qn7__PF09au6e7i5vbq8qxzURlZhqbFpda2WAAZRS-OdFLwBaIUSQaM3oACF0D4oUTaUjdfMtxACtg4MnJDvW971vOx96_wwjdjZ9Rh7HF9twmg_IkP8Y1fpr-VFBimgEHzbEYzpZfZ5sn3Mznfd9kO20bwGqTaT9LbQjSnn0Yf9EM7sxgT7ZoLdm2D_m1Bav75fct-4U73gZzscs8MujEXxmPdljeJKMQ3_AIuSj4w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78153469</pqid></control><display><type>article</type><title>Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>KHEIR, S. M ; BINES, S. D ; VONROENN, J. H ; SENG-JAW SOONG ; URIST, M. M ; COON, J. S</creator><creatorcontrib>KHEIR, S. M ; BINES, S. D ; VONROENN, J. H ; SENG-JAW SOONG ; URIST, M. M ; COON, J. S</creatorcontrib><description>The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma.</description><identifier>ISSN: 0003-4932</identifier><identifier>EISSN: 1528-1140</identifier><identifier>DOI: 10.1097/00000658-198804000-00014</identifier><identifier>PMID: 3355269</identifier><identifier>CODEN: ANSUA5</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Actuarial Analysis ; Aneuploidy ; Biological and medical sciences ; Dermatology ; DNA, Neoplasm - analysis ; Flow Cytometry ; Humans ; Medical sciences ; Melanoma - genetics ; Melanoma - mortality ; Neoplasm Recurrence, Local ; Prognosis ; Skin Neoplasms - genetics ; Skin Neoplasms - mortality ; Statistics as Topic ; Tumors of the skin and soft tissue. Premalignant lesions</subject><ispartof>Annals of surgery, 1988-04, Vol.207 (4), p.455-461</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3594-fb8a7d856b339aa849ec421733d262f8ae9363a228ef6226947e80ed3ffadc393</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1493423/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1493423/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=7616608$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3355269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KHEIR, S. M</creatorcontrib><creatorcontrib>BINES, S. D</creatorcontrib><creatorcontrib>VONROENN, J. H</creatorcontrib><creatorcontrib>SENG-JAW SOONG</creatorcontrib><creatorcontrib>URIST, M. M</creatorcontrib><creatorcontrib>COON, J. S</creatorcontrib><title>Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma</title><title>Annals of surgery</title><addtitle>Ann Surg</addtitle><description>The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma.</description><subject>Actuarial Analysis</subject><subject>Aneuploidy</subject><subject>Biological and medical sciences</subject><subject>Dermatology</subject><subject>DNA, Neoplasm - analysis</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Melanoma - genetics</subject><subject>Melanoma - mortality</subject><subject>Neoplasm Recurrence, Local</subject><subject>Prognosis</subject><subject>Skin Neoplasms - genetics</subject><subject>Skin Neoplasms - mortality</subject><subject>Statistics as Topic</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><issn>0003-4932</issn><issn>1528-1140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUcFO3DAQtVARXSifUMkH1FvA9jiOfamEoKVICDjQszXr2FujJF7ipBJ_X293WYEly5p5M2_G7xFCOTvnzDQXbHNUrStutGayBFW5XB6QBa9FSXPJPpFFyUElDYjP5Djn502FZs0ROQKoa6HMgtw8jmk1pDxFR3NcDTFEh4PzNAV6fX9JcfDzukuxfaVxoHnClae31M1TAdKcae87HFKPX8hhwC770917Qn7__PF09au6e7i5vbq8qxzURlZhqbFpda2WAAZRS-OdFLwBaIUSQaM3oACF0D4oUTaUjdfMtxACtg4MnJDvW971vOx96_wwjdjZ9Rh7HF9twmg_IkP8Y1fpr-VFBimgEHzbEYzpZfZ5sn3Mznfd9kO20bwGqTaT9LbQjSnn0Yf9EM7sxgT7ZoLdm2D_m1Bav75fct-4U73gZzscs8MujEXxmPdljeJKMQ3_AIuSj4w</recordid><startdate>19880401</startdate><enddate>19880401</enddate><creator>KHEIR, S. M</creator><creator>BINES, S. D</creator><creator>VONROENN, J. H</creator><creator>SENG-JAW SOONG</creator><creator>URIST, M. M</creator><creator>COON, J. S</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19880401</creationdate><title>Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma</title><author>KHEIR, S. M ; BINES, S. D ; VONROENN, J. H ; SENG-JAW SOONG ; URIST, M. M ; COON, J. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3594-fb8a7d856b339aa849ec421733d262f8ae9363a228ef6226947e80ed3ffadc393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Actuarial Analysis</topic><topic>Aneuploidy</topic><topic>Biological and medical sciences</topic><topic>Dermatology</topic><topic>DNA, Neoplasm - analysis</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Melanoma - genetics</topic><topic>Melanoma - mortality</topic><topic>Neoplasm Recurrence, Local</topic><topic>Prognosis</topic><topic>Skin Neoplasms - genetics</topic><topic>Skin Neoplasms - mortality</topic><topic>Statistics as Topic</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KHEIR, S. M</creatorcontrib><creatorcontrib>BINES, S. D</creatorcontrib><creatorcontrib>VONROENN, J. H</creatorcontrib><creatorcontrib>SENG-JAW SOONG</creatorcontrib><creatorcontrib>URIST, M. M</creatorcontrib><creatorcontrib>COON, J. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KHEIR, S. M</au><au>BINES, S. D</au><au>VONROENN, J. H</au><au>SENG-JAW SOONG</au><au>URIST, M. M</au><au>COON, J. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma</atitle><jtitle>Annals of surgery</jtitle><addtitle>Ann Surg</addtitle><date>1988-04-01</date><risdate>1988</risdate><volume>207</volume><issue>4</issue><spage>455</spage><epage>461</epage><pages>455-461</pages><issn>0003-4932</issn><eissn>1528-1140</eissn><coden>ANSUA5</coden><abstract>The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>3355269</pmid><doi>10.1097/00000658-198804000-00014</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0003-4932
ispartof Annals of surgery, 1988-04, Vol.207 (4), p.455-461
issn 0003-4932
1528-1140
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1493423
source MEDLINE; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central
subjects Actuarial Analysis
Aneuploidy
Biological and medical sciences
Dermatology
DNA, Neoplasm - analysis
Flow Cytometry
Humans
Medical sciences
Melanoma - genetics
Melanoma - mortality
Neoplasm Recurrence, Local
Prognosis
Skin Neoplasms - genetics
Skin Neoplasms - mortality
Statistics as Topic
Tumors of the skin and soft tissue. Premalignant lesions
title Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T06%3A22%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prognostic%20significance%20of%20DNA%20aneuploidy%20in%20stage%20I%20cutaneous%20melanoma&rft.jtitle=Annals%20of%20surgery&rft.au=KHEIR,%20S.%20M&rft.date=1988-04-01&rft.volume=207&rft.issue=4&rft.spage=455&rft.epage=461&rft.pages=455-461&rft.issn=0003-4932&rft.eissn=1528-1140&rft.coden=ANSUA5&rft_id=info:doi/10.1097/00000658-198804000-00014&rft_dat=%3Cproquest_pubme%3E78153469%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78153469&rft_id=info:pmid/3355269&rfr_iscdi=true