Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma
The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35...
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Veröffentlicht in: | Annals of surgery 1988-04, Vol.207 (4), p.455-461 |
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description | The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma. |
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M ; BINES, S. D ; VONROENN, J. H ; SENG-JAW SOONG ; URIST, M. M ; COON, J. S</creator><creatorcontrib>KHEIR, S. M ; BINES, S. D ; VONROENN, J. H ; SENG-JAW SOONG ; URIST, M. M ; COON, J. S</creatorcontrib><description>The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma.</description><identifier>ISSN: 0003-4932</identifier><identifier>EISSN: 1528-1140</identifier><identifier>DOI: 10.1097/00000658-198804000-00014</identifier><identifier>PMID: 3355269</identifier><identifier>CODEN: ANSUA5</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Actuarial Analysis ; Aneuploidy ; Biological and medical sciences ; Dermatology ; DNA, Neoplasm - analysis ; Flow Cytometry ; Humans ; Medical sciences ; Melanoma - genetics ; Melanoma - mortality ; Neoplasm Recurrence, Local ; Prognosis ; Skin Neoplasms - genetics ; Skin Neoplasms - mortality ; Statistics as Topic ; Tumors of the skin and soft tissue. 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M</creatorcontrib><creatorcontrib>BINES, S. D</creatorcontrib><creatorcontrib>VONROENN, J. H</creatorcontrib><creatorcontrib>SENG-JAW SOONG</creatorcontrib><creatorcontrib>URIST, M. M</creatorcontrib><creatorcontrib>COON, J. S</creatorcontrib><title>Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma</title><title>Annals of surgery</title><addtitle>Ann Surg</addtitle><description>The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma.</description><subject>Actuarial Analysis</subject><subject>Aneuploidy</subject><subject>Biological and medical sciences</subject><subject>Dermatology</subject><subject>DNA, Neoplasm - analysis</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Melanoma - genetics</subject><subject>Melanoma - mortality</subject><subject>Neoplasm Recurrence, Local</subject><subject>Prognosis</subject><subject>Skin Neoplasms - genetics</subject><subject>Skin Neoplasms - mortality</subject><subject>Statistics as Topic</subject><subject>Tumors of the skin and soft tissue. 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S</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19880401</creationdate><title>Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma</title><author>KHEIR, S. M ; BINES, S. D ; VONROENN, J. H ; SENG-JAW SOONG ; URIST, M. M ; COON, J. 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D</creatorcontrib><creatorcontrib>VONROENN, J. H</creatorcontrib><creatorcontrib>SENG-JAW SOONG</creatorcontrib><creatorcontrib>URIST, M. M</creatorcontrib><creatorcontrib>COON, J. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KHEIR, S. M</au><au>BINES, S. D</au><au>VONROENN, J. H</au><au>SENG-JAW SOONG</au><au>URIST, M. M</au><au>COON, J. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma</atitle><jtitle>Annals of surgery</jtitle><addtitle>Ann Surg</addtitle><date>1988-04-01</date><risdate>1988</risdate><volume>207</volume><issue>4</issue><spage>455</spage><epage>461</epage><pages>455-461</pages><issn>0003-4932</issn><eissn>1528-1140</eissn><coden>ANSUA5</coden><abstract>The prognostic significance of DNA aneuploidy was studied restrospectively in 177 Stage I cutaneous melanomas. DNA content was determined by flow cytometry of propidium iodide-stained nuclei recovered from formalin-fixed, paraffin-embedded material. Of 162 evaluable histograms, 124 were diploid, 35 aneuploid, and 3 tetraploid. Aneuploidy strongly correlated with established predictors of unfavorable prognosis, namely, thickness p less than .005, level p less than 0.005, ulceration p less than 0.005, and presence of vertical growth phase p less than 0.02. Overall, aneuploidy was strongly correlated with recurrence (p less than 0.005) and shorter disease-free survival (p less than 0.0001). Aneuploidy was an independent predictor of recurrence for tumors less than 1.5 mm thick (p less than 0.0001) and greater than or equal to 3 mm thick (p = 0.031). For melanomas 1.5-2.9 mm thick, aneuploid tumors had a 27% higher recurrence rate than diploid tumors (63% vs. 36%). This was not statistically significant (p = 0.247). In a multivariate analysis of common predictors stratified by thickness, DNA aneuploidy was the most significant independent parameter (p less than 0.002). DNA content appears to be an important stratification parameter for Stage I cutaneous melanoma.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>3355269</pmid><doi>10.1097/00000658-198804000-00014</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Actuarial Analysis Aneuploidy Biological and medical sciences Dermatology DNA, Neoplasm - analysis Flow Cytometry Humans Medical sciences Melanoma - genetics Melanoma - mortality Neoplasm Recurrence, Local Prognosis Skin Neoplasms - genetics Skin Neoplasms - mortality Statistics as Topic Tumors of the skin and soft tissue. Premalignant lesions |
title | Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma |
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