A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes
A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs...
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| Veröffentlicht in: | Nucleic acids research 1999-09, Vol.27 (17), p.e15-15 |
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| container_issue | 17 |
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| container_title | Nucleic acids research |
| container_volume | 27 |
| creator | van Deursen, P B Gunther, A W van Riel, C C van der Eijnden, M M Vos, H L van Gemen, B van Strijp, D A Tackent, N M Bertina, R M |
| description | A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs in human monocytes that were stimulated with LPS, the multiplex Q-NASBA proved to be a successful tool to monitor the expression levels of two individual mRNAs in a single-tube amplification system. The method has potential in all fields in which quantitative information is needed on two individual RNAs. |
| doi_str_mv | 10.1093/nar/27.17.e15 |
| format | Article |
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The method has potential in all fields in which quantitative information is needed on two individual RNAs.</description><subject>Biological Assay - methods</subject><subject>Cells, Cultured</subject><subject>Genetic Markers</subject><subject>Humans</subject><subject>Lipopolysaccharide Receptors - genetics</subject><subject>Lipopolysaccharides - immunology</subject><subject>Monocytes - immunology</subject><subject>Monocytes - metabolism</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Reproducibility of Results</subject><subject>RNA, Messenger - analysis</subject><subject>Thromboplastin - genetics</subject><subject>Time Factors</subject><issn>1362-4962</issn><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1v1DAQxSMEoqVw5Ip84patJ3bsBIlDunxKVZGgd8vrTLpGjp3azoqKfx6jrdBymqeZ35sZ6VXVa6AboD279DpeNnIDcoPQPqnOgYmm5r1onp7os-pFSj8pBQ4tf16dAeVcNG13Xv0eiA8HdOR-1T7brLM9IJlXl-3i8Be5GX5cDWTGvA_jO6KXxVlTmOBJDqWt0xqtvyPZprQimbTJIRLtR7L9AJzM328G4rDsT8R6sl9n7ckcfDAPGdPL6tmkXcJXj_Wiuv308Xb7pb7-9vnrdriuDROsrXdgGtZSxpCNrW4FAylhnMZR9Ax2nWCikwhSFClQ80mMlNJm4mAYY2bHLqr3x7XLuptxNOhz1E4t0c46Pqigrfp_4u1e3YWDAt61UhT_20d_DPcrpqxmmww6pz2GNSnR94J1oilgfQRNDClFnP7dAKr-hqVKWKqRCqQqYRX-zeljJ_QxHfYHXN6STg</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>van Deursen, P B</creator><creator>Gunther, A W</creator><creator>van Riel, C C</creator><creator>van der Eijnden, M M</creator><creator>Vos, H L</creator><creator>van Gemen, B</creator><creator>van Strijp, D A</creator><creator>Tackent, N M</creator><creator>Bertina, R M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990901</creationdate><title>A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes</title><author>van Deursen, P B ; Gunther, A W ; van Riel, C C ; van der Eijnden, M M ; Vos, H L ; van Gemen, B ; van Strijp, D A ; Tackent, N M ; Bertina, R M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3635-b1c235033e3d5a5631771dfdd6931b863687e176b866ea4f6d0002f41c333cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological Assay - methods</topic><topic>Cells, Cultured</topic><topic>Genetic Markers</topic><topic>Humans</topic><topic>Lipopolysaccharide Receptors - genetics</topic><topic>Lipopolysaccharides - immunology</topic><topic>Monocytes - immunology</topic><topic>Monocytes - metabolism</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Reproducibility of Results</topic><topic>RNA, Messenger - analysis</topic><topic>Thromboplastin - genetics</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Deursen, P B</creatorcontrib><creatorcontrib>Gunther, A W</creatorcontrib><creatorcontrib>van Riel, C C</creatorcontrib><creatorcontrib>van der Eijnden, M M</creatorcontrib><creatorcontrib>Vos, H L</creatorcontrib><creatorcontrib>van Gemen, B</creatorcontrib><creatorcontrib>van Strijp, D A</creatorcontrib><creatorcontrib>Tackent, N M</creatorcontrib><creatorcontrib>Bertina, R M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Deursen, P B</au><au>Gunther, A W</au><au>van Riel, C C</au><au>van der Eijnden, M M</au><au>Vos, H L</au><au>van Gemen, B</au><au>van Strijp, D A</au><au>Tackent, N M</au><au>Bertina, R M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>27</volume><issue>17</issue><spage>e15</spage><epage>15</epage><pages>e15-15</pages><issn>1362-4962</issn><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs in human monocytes that were stimulated with LPS, the multiplex Q-NASBA proved to be a successful tool to monitor the expression levels of two individual mRNAs in a single-tube amplification system. The method has potential in all fields in which quantitative information is needed on two individual RNAs.</abstract><cop>England</cop><pmid>10446258</pmid><doi>10.1093/nar/27.17.e15</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1362-4962 |
| ispartof | Nucleic acids research, 1999-09, Vol.27 (17), p.e15-15 |
| issn | 1362-4962 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_148576 |
| source | Oxford Journals Open Access Collection; MEDLINE; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biological Assay - methods Cells, Cultured Genetic Markers Humans Lipopolysaccharide Receptors - genetics Lipopolysaccharides - immunology Monocytes - immunology Monocytes - metabolism Nucleic Acid Amplification Techniques Reproducibility of Results RNA, Messenger - analysis Thromboplastin - genetics Time Factors |
| title | A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes |
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