Identification of genes regulated by muscarinic acetylcholine receptors: application of an improved and statistically comprehensive mRNA differential display technique

In order to identify genes that are regulated by muscarinic acetylcholine receptors, we developed an mRNA differential display technique (DD) approach. By increasing redundancy and by evaluating optimised reagents and conditions for reverse transcription of total RNA, PCR and separation of PCR produ...

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Veröffentlicht in:	Nucleic acids research 1999-05, Vol.27 (10), p.2211-2218
Hauptverfasser:	von der Kammer, Heinz, Albrecht, Claudia, Mayhaus, Manuel, Hoffmann, Barbara, Stanke, Gesa, Nitsch, Roger M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence c-jun gene Cell Line DNA Primers - genetics Egr-1 gene Egr-2 gene Egr-3 gene ETR101 gene Gene Expression Regulation Genes, Immediate-Early Gig-2 gene Gos-3 gene hcyr61 gene Humans jun-D gene mRNA NGFi-B gene Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data Receptor, Muscarinic M1 Receptors, Muscarinic - genetics RNA, Messenger - genetics Transfection
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