Simultaneous, coincident optical trapping and single-molecule fluorescence

We constructed a microscope-based instrument capable of simultaneous, spatially coincident optical trapping and single-molecule fluorescence. The capabilities of this apparatus were demonstrated by studying the force-induced strand separation of a dye-labeled, 15-base-pair region of double-stranded...

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Veröffentlicht in:	Nature methods 2004-11, Vol.1 (2), p.133-139
Hauptverfasser:	Block, Steven M, Lang, Matthew J, Fordyce, Polly M, Engh, Anita M, Neuman, Keir C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Deoxyribonucleic acid DNA DNA - analysis DNA - chemistry Elasticity Emissions Equipment Design Equipment Failure Analysis Fluorescence Genetic research Health aspects Lasers Load distribution Medical equipment Methods Micromanipulation - instrumentation Micromanipulation - methods Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Microscopy, Video - instrumentation Microscopy, Video - methods Physical Stimulation - instrumentation Physical Stimulation - methods Physiological apparatus Physiological aspects Stress, Mechanical Systems Integration Trapping
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