Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells
Using the modified screening approach in combination with expressed sequence tags, we have identified several novel cDNAs from mouse embryonic stem (ES) cells, whose expression is tissue-restricted and/or developmentally regulated. One of the cDNAs, ES18, is preferentially expressed in lymph node an...
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| Veröffentlicht in: | Nucleic acids research 1999-03, Vol.27 (6), p.1524-1530 |
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| creator | Park, Eun Jung Kim, Jin Hee Seong, Rho Hyun Kim, Chul Geun Park, Sang Dai Hong, Seung Hwan |
| description | Using the modified screening approach in combination with expressed sequence tags, we have identified several novel cDNAs from mouse embryonic stem (ES) cells, whose expression is tissue-restricted and/or developmentally regulated. One of the cDNAs, ES18, is preferentially expressed in lymph node and thymus, and contains noteworthy features of transcriptional regulator. The expression of ES18 transcript was selectively regulated during the apoptosis of T-cell thymoma S49.1 induced by several stimuli. Interestingly, the ES18 transcript was differently regulated in the mutually antagonistic process, between dexamethasone- and A23187-induced cell death of T-cells. Moreover, the message level of ES18 was selectively enhanced by staurosporine, a broad protein kinase inhibitor, but not by other protein kinase inhibitors such as GF109203X and H89. In addition, ES18 transcript was induced by C2-ceramide, which is a mediator of both dexamethasone- and staurosporine-induced apoptotic signaling. We further showed that transient overexpression of ES18 in mouse T-cell lymphoma increased the apoptotic cell death. These data suggest that ES18 may be selectively involved in specific apoptotic processes in mouse T-cells. |
| doi_str_mv | 10.1093/nar/27.6.1524 |
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One of the cDNAs, ES18, is preferentially expressed in lymph node and thymus, and contains noteworthy features of transcriptional regulator. The expression of ES18 transcript was selectively regulated during the apoptosis of T-cell thymoma S49.1 induced by several stimuli. Interestingly, the ES18 transcript was differently regulated in the mutually antagonistic process, between dexamethasone- and A23187-induced cell death of T-cells. Moreover, the message level of ES18 was selectively enhanced by staurosporine, a broad protein kinase inhibitor, but not by other protein kinase inhibitors such as GF109203X and H89. In addition, ES18 transcript was induced by C2-ceramide, which is a mediator of both dexamethasone- and staurosporine-induced apoptotic signaling. We further showed that transient overexpression of ES18 in mouse T-cell lymphoma increased the apoptotic cell death. These data suggest that ES18 may be selectively involved in specific apoptotic processes in mouse T-cells.</description><identifier>ISSN: 0305-1048</identifier><identifier>ISSN: 1362-4962</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/27.6.1524</identifier><identifier>PMID: 10037816</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Amino Acid Sequence ; Animals ; Apoptosis - genetics ; Apoptosis Regulatory Proteins ; Base Sequence ; Cell death ; Ceramides - pharmacology ; Cloning, Molecular ; DNA, Complementary - genetics ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Neoplastic ; Immune System ; Mice ; Molecular Sequence Data ; Protein Kinase C - antagonists & inhibitors ; Staurosporine - pharmacology ; Stem Cells ; T-Lymphocytes - pathology ; Thymoma ; Thymus Gland ; Tissue Distribution ; Transcription Factors - genetics</subject><ispartof>Nucleic acids research, 1999-03, Vol.27 (6), p.1524-1530</ispartof><rights>Copyright Oxford University Press(England) Mar 15, 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-191e2f5f088ec8bd669d14ed777e54d863f9e9bafe60c79c4e13b92e406c913d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC148348/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC148348/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10037816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Eun Jung</creatorcontrib><creatorcontrib>Kim, Jin Hee</creatorcontrib><creatorcontrib>Seong, Rho Hyun</creatorcontrib><creatorcontrib>Kim, Chul Geun</creatorcontrib><creatorcontrib>Park, Sang Dai</creatorcontrib><creatorcontrib>Hong, Seung Hwan</creatorcontrib><title>Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Research</addtitle><description>Using the modified screening approach in combination with expressed sequence tags, we have identified several novel cDNAs from mouse embryonic stem (ES) cells, whose expression is tissue-restricted and/or developmentally regulated. One of the cDNAs, ES18, is preferentially expressed in lymph node and thymus, and contains noteworthy features of transcriptional regulator. The expression of ES18 transcript was selectively regulated during the apoptosis of T-cell thymoma S49.1 induced by several stimuli. Interestingly, the ES18 transcript was differently regulated in the mutually antagonistic process, between dexamethasone- and A23187-induced cell death of T-cells. Moreover, the message level of ES18 was selectively enhanced by staurosporine, a broad protein kinase inhibitor, but not by other protein kinase inhibitors such as GF109203X and H89. In addition, ES18 transcript was induced by C2-ceramide, which is a mediator of both dexamethasone- and staurosporine-induced apoptotic signaling. We further showed that transient overexpression of ES18 in mouse T-cell lymphoma increased the apoptotic cell death. These data suggest that ES18 may be selectively involved in specific apoptotic processes in mouse T-cells.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Apoptosis - genetics</subject><subject>Apoptosis Regulatory Proteins</subject><subject>Base Sequence</subject><subject>Cell death</subject><subject>Ceramides - pharmacology</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Immune System</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Staurosporine - pharmacology</subject><subject>Stem Cells</subject><subject>T-Lymphocytes - pathology</subject><subject>Thymoma</subject><subject>Thymus Gland</subject><subject>Tissue Distribution</subject><subject>Transcription Factors - genetics</subject><issn>0305-1048</issn><issn>1362-4962</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi0EoiHlyBVZHHrqph7b648DhyqUFlHRQ0sFXCzHO0u2bNbB3kTQX99dpSqFC5KlsTXPzPidl5BXwGbArDjqfDrieqZmUHL5hExAKF5Iq_hTMmGClQUwafbIi5xvGAMJpXxO9oAxoQ2oCfk6X_rkQ4-pufV9Ezsaa-ppF7fY0lXcZKTh3afjQ3pyCeaQNt02tlushgv167juY98EGrBtaYW-X47VV8X4zvvkWe3bjC_v45R8fn9yNT8rzi9OP8yPz4sgtekLsIC8LmtmDAazqJSyFUistNZYysooUVu0C1-jYkHbIBHEwnKUTAULohJT8nbXd71ZrLAK2PXJt26dmpVPv130jfs70zVL9z1uHUgjhjMlB_f1Kf7cYO7dqsmjAt_hoN8pW1oxTPovCJqDsZwN4Jt_wJu4Sd2wBMcZU6CUHKFiB4UUc05YP_wYmBuddYOzjmun3OjswL9-LPMRvbPyT8Mm9_jrIe_TD6e00KU7-_LNmetL8xHsqbsWdzVcrbU</recordid><startdate>19990315</startdate><enddate>19990315</enddate><creator>Park, Eun Jung</creator><creator>Kim, Jin Hee</creator><creator>Seong, Rho Hyun</creator><creator>Kim, Chul Geun</creator><creator>Park, Sang Dai</creator><creator>Hong, Seung Hwan</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990315</creationdate><title>Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells</title><author>Park, Eun Jung ; Kim, Jin Hee ; Seong, Rho Hyun ; Kim, Chul Geun ; Park, Sang Dai ; Hong, Seung Hwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-191e2f5f088ec8bd669d14ed777e54d863f9e9bafe60c79c4e13b92e406c913d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Apoptosis - genetics</topic><topic>Apoptosis Regulatory Proteins</topic><topic>Base Sequence</topic><topic>Cell death</topic><topic>Ceramides - pharmacology</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Immune System</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Staurosporine - pharmacology</topic><topic>Stem Cells</topic><topic>T-Lymphocytes - pathology</topic><topic>Thymoma</topic><topic>Thymus Gland</topic><topic>Tissue Distribution</topic><topic>Transcription Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Eun Jung</creatorcontrib><creatorcontrib>Kim, Jin Hee</creatorcontrib><creatorcontrib>Seong, Rho Hyun</creatorcontrib><creatorcontrib>Kim, Chul Geun</creatorcontrib><creatorcontrib>Park, Sang Dai</creatorcontrib><creatorcontrib>Hong, Seung Hwan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Eun Jung</au><au>Kim, Jin Hee</au><au>Seong, Rho Hyun</au><au>Kim, Chul Geun</au><au>Park, Sang Dai</au><au>Hong, Seung Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Research</addtitle><date>1999-03-15</date><risdate>1999</risdate><volume>27</volume><issue>6</issue><spage>1524</spage><epage>1530</epage><pages>1524-1530</pages><issn>0305-1048</issn><issn>1362-4962</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Using the modified screening approach in combination with expressed sequence tags, we have identified several novel cDNAs from mouse embryonic stem (ES) cells, whose expression is tissue-restricted and/or developmentally regulated. One of the cDNAs, ES18, is preferentially expressed in lymph node and thymus, and contains noteworthy features of transcriptional regulator. The expression of ES18 transcript was selectively regulated during the apoptosis of T-cell thymoma S49.1 induced by several stimuli. Interestingly, the ES18 transcript was differently regulated in the mutually antagonistic process, between dexamethasone- and A23187-induced cell death of T-cells. Moreover, the message level of ES18 was selectively enhanced by staurosporine, a broad protein kinase inhibitor, but not by other protein kinase inhibitors such as GF109203X and H89. In addition, ES18 transcript was induced by C2-ceramide, which is a mediator of both dexamethasone- and staurosporine-induced apoptotic signaling. We further showed that transient overexpression of ES18 in mouse T-cell lymphoma increased the apoptotic cell death. These data suggest that ES18 may be selectively involved in specific apoptotic processes in mouse T-cells.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>10037816</pmid><doi>10.1093/nar/27.6.1524</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals Apoptosis - genetics Apoptosis Regulatory Proteins Base Sequence Cell death Ceramides - pharmacology Cloning, Molecular DNA, Complementary - genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Neoplastic Immune System Mice Molecular Sequence Data Protein Kinase C - antagonists & inhibitors Staurosporine - pharmacology Stem Cells T-Lymphocytes - pathology Thymoma Thymus Gland Tissue Distribution Transcription Factors - genetics |
| title | Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells |
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