The two subunits of human molybdopterin synthase: Evidence for a bicistronic messenger RNA with overlapping reading frames
Molybdoenzymes are ubiquitous and require a prosthetic group called the molybdenum cofactor for activity. We provide evidence here that the two heteromeric subunits (MOCO1-A and MOCO1-B) of human molybdopterin synthase, which is involved in the conversion of precursor Z to molybdopterin in the molyb...
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| Veröffentlicht in: | Nucleic acids research 1999-02, Vol.27 (3), p.854-858 |
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| creator | Sloan, Joan Kinghorn, James R. Unkles, Shiela E. |
| description | Molybdoenzymes are ubiquitous and require a prosthetic group called the molybdenum cofactor for activity. We provide evidence here that the two heteromeric subunits (MOCO1-A and MOCO1-B) of human molybdopterin synthase, which is involved in the conversion of precursor Z to molybdopterin in the molybdenum cofactor biosynthetic pathway, are specified by a single bicistronic mRNA with overlapping reading frames. The transcript is in low abundance and shows variable tissue distribution. We propose that leaky scanning of the first translational initiation codon for MOCO1-A by 40S ribosomal subunits occurs, allowing recognition of the AUG for the downstream MOCO1-B reading frame. Such a genetic arrangement may result in a constant ratio and close proximity of lowly expressed enzyme subunits which should, a priori, be especially advantageous for assembly in complex mammalian cells. The MOCO1 locus resides on human chromosome 5. |
| doi_str_mv | 10.1093/nar/27.3.854 |
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We provide evidence here that the two heteromeric subunits (MOCO1-A and MOCO1-B) of human molybdopterin synthase, which is involved in the conversion of precursor Z to molybdopterin in the molybdenum cofactor biosynthetic pathway, are specified by a single bicistronic mRNA with overlapping reading frames. The transcript is in low abundance and shows variable tissue distribution. We propose that leaky scanning of the first translational initiation codon for MOCO1-A by 40S ribosomal subunits occurs, allowing recognition of the AUG for the downstream MOCO1-B reading frame. Such a genetic arrangement may result in a constant ratio and close proximity of lowly expressed enzyme subunits which should, a priori, be especially advantageous for assembly in complex mammalian cells. 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The MOCO1 locus resides on human chromosome 5.</description><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Aspergillus nidulans - enzymology</subject><subject>Aspergillus nidulans - genetics</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Expressed Sequence Tags</subject><subject>Female</subject><subject>Humans</subject><subject>Liver - embryology</subject><subject>Liver - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Peptide Fragments - genetics</subject><subject>Pregnancy</subject><subject>Protein Conformation</subject><subject>RNA, Messenger - chemistry</subject><subject>Sequence Alignment</subject><subject>Spleen - embryology</subject><subject>Spleen - metabolism</subject><subject>Sulfurtransferases - genetics</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQRiMEKkvhxhXJ4sCJbO3YiR2kHqqqpYiqSKhIiIvlxOONS2IHO9l2--vxalcr4MJpDt-b0cy8LHtN8JLgmp44FU4KvqRLUbIn2YLQqshZXRVPswWmuMwJZuJ59iLGO4wJIyU7yo5qIepC0EX2eNsBmu49inMzOztF5A3q5kE5NPh-02g_ThCsQ3Hjpk5F-IAu1laDawEZH5BCjW1tnIJ3tkUDxAhuBQF9vTlD93bqkF9D6NU4WrdCAZTeVhNUIl9mz4zqI7za1-Ps2-XF7flVfv3l46fzs-u8ZZxNeQ2gjcKGa0U0TusroQXTBS-VqRqFgWkiGOMtMCKIJlQLzkRhABMDtCT0ODvdzR3nZgDdgpuC6uUY7KDCRnpl5d-Js51c-bUkaUzJU_-7fX_wv2aIkxxsbKHvlQM_R1nVJeOkrP4LEl7Qiost-PYf8M7PwaUnyALjim4PSND7HdQGH2MAc9iYYLkVL5N4WXBJZRKf8Dd_XnmA96ZTnu_yJAseDrEKP2XFKS_l1fcfEvP65vNlXUpOfwM7gLuO</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Sloan, Joan</creator><creator>Kinghorn, James R.</creator><creator>Unkles, Shiela E.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199902</creationdate><title>The two subunits of human molybdopterin synthase: Evidence for a bicistronic messenger RNA with overlapping reading frames</title><author>Sloan, Joan ; Kinghorn, James R. ; Unkles, Shiela E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-9eedfa0f7da1d0154a8d84d275af6ba0e4d18447ce4181d13d87482fe01fe3513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Amino Acid Sequence</topic><topic>Aspergillus nidulans - enzymology</topic><topic>Aspergillus nidulans - genetics</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Expressed Sequence Tags</topic><topic>Female</topic><topic>Humans</topic><topic>Liver - embryology</topic><topic>Liver - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Peptide Fragments - genetics</topic><topic>Pregnancy</topic><topic>Protein Conformation</topic><topic>RNA, Messenger - chemistry</topic><topic>Sequence Alignment</topic><topic>Spleen - embryology</topic><topic>Spleen - metabolism</topic><topic>Sulfurtransferases - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sloan, Joan</creatorcontrib><creatorcontrib>Kinghorn, James R.</creatorcontrib><creatorcontrib>Unkles, Shiela E.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sloan, Joan</au><au>Kinghorn, James R.</au><au>Unkles, Shiela E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The two subunits of human molybdopterin synthase: Evidence for a bicistronic messenger RNA with overlapping reading frames</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Research</addtitle><date>1999-02</date><risdate>1999</risdate><volume>27</volume><issue>3</issue><spage>854</spage><epage>858</epage><pages>854-858</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Molybdoenzymes are ubiquitous and require a prosthetic group called the molybdenum cofactor for activity. We provide evidence here that the two heteromeric subunits (MOCO1-A and MOCO1-B) of human molybdopterin synthase, which is involved in the conversion of precursor Z to molybdopterin in the molybdenum cofactor biosynthetic pathway, are specified by a single bicistronic mRNA with overlapping reading frames. The transcript is in low abundance and shows variable tissue distribution. We propose that leaky scanning of the first translational initiation codon for MOCO1-A by 40S ribosomal subunits occurs, allowing recognition of the AUG for the downstream MOCO1-B reading frame. Such a genetic arrangement may result in a constant ratio and close proximity of lowly expressed enzyme subunits which should, a priori, be especially advantageous for assembly in complex mammalian cells. 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| subjects | Adult Amino Acid Sequence Aspergillus nidulans - enzymology Aspergillus nidulans - genetics Base Sequence Blotting, Northern Expressed Sequence Tags Female Humans Liver - embryology Liver - metabolism Molecular Sequence Data Open Reading Frames Peptide Fragments - genetics Pregnancy Protein Conformation RNA, Messenger - chemistry Sequence Alignment Spleen - embryology Spleen - metabolism Sulfurtransferases - genetics |
| title | The two subunits of human molybdopterin synthase: Evidence for a bicistronic messenger RNA with overlapping reading frames |
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