A convenient method of aligning large DNA molecules on bare mica surfaces for atomic force microscopy
Large DNA molecules remain difficult to be imaged by atomic force microscopy (AFM) because of the tendency of aggregation. A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. The clear background, minimal overstretching, high reproducibi...
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| Veröffentlicht in: | Nucleic acids research 1998-10, Vol.26 (20), p.4785-4786 |
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| container_end_page | 4786 |
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| container_issue | 20 |
| container_start_page | 4785 |
| container_title | Nucleic acids research |
| container_volume | 26 |
| creator | Li, Jianwei Bai, Chunli Wang, Chen Zhu, Chuanfeng Lin, Zhang Li, Qing Cao, Enhua |
| description | Large DNA molecules remain difficult to be imaged by atomic force microscopy (AFM) because of the tendency of aggregation. A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. The clear background, minimal overstretching, high reproducibility and convenience of this aligning procedure make it useful for physical mapping of genome regions and the studies of DNA-protein complexes. |
| doi_str_mv | 10.1093/nar/26.20.4785 |
| format | Article |
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A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. 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A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. 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| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1998-10, Vol.26 (20), p.4785-4786 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_147909 |
| source | MEDLINE; Oxford Journals Open Access Collection; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aluminum Silicates Bacteriophage lambda - genetics DNA - genetics DNA - metabolism DNA - ultrastructure DNA-Binding Proteins - metabolism Microscopy, Atomic Force - methods Physical Chromosome Mapping Reproducibility of Results Time Factors |
| title | A convenient method of aligning large DNA molecules on bare mica surfaces for atomic force microscopy |
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