Osteoblast response to zirconia-hybridized pyrophosphate-stabilized amorphous calcium phosphate
Calcium phosphate bioceramics, such as hydroxyapatite, have long been used as bone substitutes because of their proven biocompatibility and bone binding properties in vivo. Recently, a zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate (Zr‐ACP) has been synthesized, which is mo...
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| Veröffentlicht in: | Journal of biomedical materials research 2006-03, Vol.76A (3), p.596-604 |
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| creator | Whited, Bryce M. Skrtic, Drago Love, Brian J. Goldstein, Aaron S. |
| description | Calcium phosphate bioceramics, such as hydroxyapatite, have long been used as bone substitutes because of their proven biocompatibility and bone binding properties in vivo. Recently, a zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate (Zr‐ACP) has been synthesized, which is more soluble than hydroxyapatite and allows for controlled release of calcium and phosphate ions. These ions have been postulated to increase osteoblast differentiation and mineralization in vitro. The focus of this work is to elucidate the physicochemical properties of Zr‐ACP and to measure cell response to Zr‐ACP in vitro using a MC3T3‐E1 mouse calvarial‐derived osteoprogenitor cell line. Cells were cultured in osteogenic medium and mineral was added to culture at different stages in cell maturation. Culture in the presence of Zr‐ACP showed significant increases in cell proliferation, alkaline phosphatase activity (ALP), and osteopontin (OPN) synthesis, whereas collagen synthesis was unaffected. In addition, calcium and phosphate ion concentrations and medium pH were found to transiently increase with the addition of Zr‐ACP, and are hypothesized to be responsible for the osteogenic effect of Zr‐ACP. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006 |
| doi_str_mv | 10.1002/jbm.a.30573 |
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Recently, a zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate (Zr‐ACP) has been synthesized, which is more soluble than hydroxyapatite and allows for controlled release of calcium and phosphate ions. These ions have been postulated to increase osteoblast differentiation and mineralization in vitro. The focus of this work is to elucidate the physicochemical properties of Zr‐ACP and to measure cell response to Zr‐ACP in vitro using a MC3T3‐E1 mouse calvarial‐derived osteoprogenitor cell line. Cells were cultured in osteogenic medium and mineral was added to culture at different stages in cell maturation. Culture in the presence of Zr‐ACP showed significant increases in cell proliferation, alkaline phosphatase activity (ALP), and osteopontin (OPN) synthesis, whereas collagen synthesis was unaffected. In addition, calcium and phosphate ion concentrations and medium pH were found to transiently increase with the addition of Zr‐ACP, and are hypothesized to be responsible for the osteogenic effect of Zr‐ACP. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</description><identifier>ISSN: 1549-3296</identifier><identifier>ISSN: 0021-9304</identifier><identifier>EISSN: 1552-4965</identifier><identifier>EISSN: 1097-4636</identifier><identifier>DOI: 10.1002/jbm.a.30573</identifier><identifier>PMID: 16278876</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>alkaline phosphatase ; amorphous phosphate ; Animals ; Bone Substitutes ; Calcium Phosphates ; Calcium Pyrophosphate - chemistry ; Cell Differentiation - physiology ; Cell Line ; Cell Proliferation ; hydroxyapatite ; Materials Testing ; MC3T3-E1 osteoblasts ; Mice ; Osteoblasts - cytology ; Osteoblasts - physiology ; Osteogenesis - physiology ; osteopontin ; Skull - cytology ; Skull - physiology ; Zirconium - chemistry</subject><ispartof>Journal of biomedical materials research, 2006-03, Vol.76A (3), p.596-604</ispartof><rights>Copyright © 2005 Wiley Periodicals, Inc.</rights><rights>(c) 2005 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5873-b7500f7a1bc41589594737ae98baa706b6ebc83252aa10ad7626722496eb34a13</citedby><cites>FETCH-LOGICAL-c5873-b7500f7a1bc41589594737ae98baa706b6ebc83252aa10ad7626722496eb34a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.30573$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.30573$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16278876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Whited, Bryce M.</creatorcontrib><creatorcontrib>Skrtic, Drago</creatorcontrib><creatorcontrib>Love, Brian J.</creatorcontrib><creatorcontrib>Goldstein, Aaron S.</creatorcontrib><title>Osteoblast response to zirconia-hybridized pyrophosphate-stabilized amorphous calcium phosphate</title><title>Journal of biomedical materials research</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Calcium phosphate bioceramics, such as hydroxyapatite, have long been used as bone substitutes because of their proven biocompatibility and bone binding properties in vivo. Recently, a zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate (Zr‐ACP) has been synthesized, which is more soluble than hydroxyapatite and allows for controlled release of calcium and phosphate ions. These ions have been postulated to increase osteoblast differentiation and mineralization in vitro. The focus of this work is to elucidate the physicochemical properties of Zr‐ACP and to measure cell response to Zr‐ACP in vitro using a MC3T3‐E1 mouse calvarial‐derived osteoprogenitor cell line. Cells were cultured in osteogenic medium and mineral was added to culture at different stages in cell maturation. Culture in the presence of Zr‐ACP showed significant increases in cell proliferation, alkaline phosphatase activity (ALP), and osteopontin (OPN) synthesis, whereas collagen synthesis was unaffected. In addition, calcium and phosphate ion concentrations and medium pH were found to transiently increase with the addition of Zr‐ACP, and are hypothesized to be responsible for the osteogenic effect of Zr‐ACP. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</description><subject>alkaline phosphatase</subject><subject>amorphous phosphate</subject><subject>Animals</subject><subject>Bone Substitutes</subject><subject>Calcium Phosphates</subject><subject>Calcium Pyrophosphate - chemistry</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>hydroxyapatite</subject><subject>Materials Testing</subject><subject>MC3T3-E1 osteoblasts</subject><subject>Mice</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - physiology</subject><subject>Osteogenesis - physiology</subject><subject>osteopontin</subject><subject>Skull - cytology</subject><subject>Skull - physiology</subject><subject>Zirconium - chemistry</subject><issn>1549-3296</issn><issn>0021-9304</issn><issn>1552-4965</issn><issn>1097-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1PVDEUxRuiAURX7M1buSFv7HdfNyZCHPxA2GBcNredDlN47_XRvlGHv97CjKNsZHWbnN89uSenCB0SPCEY07fXtpvAhGGh2A7aJ0LQmmspnt2_ua4Z1XIPvcj5usASC7qL9oikqmmU3EfmIo8-2hbyWCWfh9hnX42xugvJxT5AvVjZFGbhzs-qYZXisIh5WMDo6zyCDe2DAF1MRVjmykHrwrKrtthL9HwObfavNvMAfZt-uDz5WJ9dnH46eX9WO9EoVlslMJ4rINZxIhotNFdMgdeNBVBYWumtaxgVFIBgmClJpaK05PSWcSDsAL1b-w5L2_mZ8_2YoDVDCh2klYkQzGOlDwtzFX8YwpUSTBeDNxuDFG-XPo-mC9n5toXel2RGYUUEaeiTINWkEZqRJ0GiOSOK8QIerUGXYs7Jz7dnE2zuKzalYgPmoeJCv_436V9202kByBr4GVq_-p-X-Xz89Y9pvd4J5Tv82u5AujGyFCHM9_NTw6d0qvn5F3PJfgN_LsNy</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Whited, Bryce M.</creator><creator>Skrtic, Drago</creator><creator>Love, Brian J.</creator><creator>Goldstein, Aaron S.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7QQ</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060301</creationdate><title>Osteoblast response to zirconia-hybridized pyrophosphate-stabilized amorphous calcium phosphate</title><author>Whited, Bryce M. ; Skrtic, Drago ; Love, Brian J. ; Goldstein, Aaron S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5873-b7500f7a1bc41589594737ae98baa706b6ebc83252aa10ad7626722496eb34a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>alkaline phosphatase</topic><topic>amorphous phosphate</topic><topic>Animals</topic><topic>Bone Substitutes</topic><topic>Calcium Phosphates</topic><topic>Calcium Pyrophosphate - chemistry</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>hydroxyapatite</topic><topic>Materials Testing</topic><topic>MC3T3-E1 osteoblasts</topic><topic>Mice</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - physiology</topic><topic>Osteogenesis - physiology</topic><topic>osteopontin</topic><topic>Skull - cytology</topic><topic>Skull - physiology</topic><topic>Zirconium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whited, Bryce M.</creatorcontrib><creatorcontrib>Skrtic, Drago</creatorcontrib><creatorcontrib>Love, Brian J.</creatorcontrib><creatorcontrib>Goldstein, Aaron S.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biomedical materials research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whited, Bryce M.</au><au>Skrtic, Drago</au><au>Love, Brian J.</au><au>Goldstein, Aaron S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteoblast response to zirconia-hybridized pyrophosphate-stabilized amorphous calcium phosphate</atitle><jtitle>Journal of biomedical materials research</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>76A</volume><issue>3</issue><spage>596</spage><epage>604</epage><pages>596-604</pages><issn>1549-3296</issn><issn>0021-9304</issn><eissn>1552-4965</eissn><eissn>1097-4636</eissn><abstract>Calcium phosphate bioceramics, such as hydroxyapatite, have long been used as bone substitutes because of their proven biocompatibility and bone binding properties in vivo. Recently, a zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate (Zr‐ACP) has been synthesized, which is more soluble than hydroxyapatite and allows for controlled release of calcium and phosphate ions. These ions have been postulated to increase osteoblast differentiation and mineralization in vitro. The focus of this work is to elucidate the physicochemical properties of Zr‐ACP and to measure cell response to Zr‐ACP in vitro using a MC3T3‐E1 mouse calvarial‐derived osteoprogenitor cell line. Cells were cultured in osteogenic medium and mineral was added to culture at different stages in cell maturation. Culture in the presence of Zr‐ACP showed significant increases in cell proliferation, alkaline phosphatase activity (ALP), and osteopontin (OPN) synthesis, whereas collagen synthesis was unaffected. In addition, calcium and phosphate ion concentrations and medium pH were found to transiently increase with the addition of Zr‐ACP, and are hypothesized to be responsible for the osteogenic effect of Zr‐ACP. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16278876</pmid><doi>10.1002/jbm.a.30573</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alkaline phosphatase amorphous phosphate Animals Bone Substitutes Calcium Phosphates Calcium Pyrophosphate - chemistry Cell Differentiation - physiology Cell Line Cell Proliferation hydroxyapatite Materials Testing MC3T3-E1 osteoblasts Mice Osteoblasts - cytology Osteoblasts - physiology Osteogenesis - physiology osteopontin Skull - cytology Skull - physiology Zirconium - chemistry |
| title | Osteoblast response to zirconia-hybridized pyrophosphate-stabilized amorphous calcium phosphate |
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