Gel electrophoretic detection of 7,8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine via oxidation by Ir(IV)

Two gel electrophoretic methods are described for detection of 7,8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine based on their further oxidation with one-electron oxidants including IrCl62− and IrBr62−. The products of nucleobase oxidation lead to enhanced piperidine-sensitive cleavage and to h...

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Veröffentlicht in:	Nucleic acids research 1998-05, Vol.26 (9), p.2247-2249
Hauptverfasser:	Muller, James G., Duarte, Victor, Hickerson, Robyn P., Burrows, Cynthia J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - analogs & derivatives Adenine - isolation & purification DNA Damage DNA Primers Electrophoresis, Polyacrylamide Gel - methods Guanine - analogs & derivatives Guanine - isolation & purification Iridium Oxidation-Reduction
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