The kinetics of thymine dimer excision in ultraviolet-irradiated human cells
We have investigated the kinetics of the loss of thymine dimers from the acid-insoluble fraction of several ultraviolet (UV)-irradiated cultured human cell lines. Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21–85 x 10(5) thymine dimers per cell and an eventual maxi...
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| Veröffentlicht in: | Biophysical journal 1978-05, Vol.22 (2), p.249-264 |
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| creator | Ehmann, U.K. Cook, K.H. Friedberg, E.C. |
| description | We have investigated the kinetics of the loss of thymine dimers from the acid-insoluble fraction of several ultraviolet (UV)-irradiated cultured human cell lines. Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21–85 x 10(5) thymine dimers per cell and an eventual maximal loss per cell of 12–20 x 10(5) thymine dimers. The time for half-maximal loss of dimers ranged from 12–22 h after UV irradiation. In contrast, the time for half-maximal repair synthesis of DNA measured by autoradiography was 4.5 h. This figure agrees well with reported half-maximal repair synthesis times, which range from 0.5 to 3.6 h based on our analysis. The discrepancy in the kinetics of the loss of thymine dimers from DNA and repair synthesis is discussed in terms of possible molecular mechanisms of thymine dimer excision in vivo and in terms of possible experimental artifacts. |
| doi_str_mv | 10.1016/S0006-3495(78)85487-3 |
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Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21–85 x 10(5) thymine dimers per cell and an eventual maximal loss per cell of 12–20 x 10(5) thymine dimers. The time for half-maximal loss of dimers ranged from 12–22 h after UV irradiation. In contrast, the time for half-maximal repair synthesis of DNA measured by autoradiography was 4.5 h. This figure agrees well with reported half-maximal repair synthesis times, which range from 0.5 to 3.6 h based on our analysis. The discrepancy in the kinetics of the loss of thymine dimers from DNA and repair synthesis is discussed in terms of possible molecular mechanisms of thymine dimer excision in vivo and in terms of possible experimental artifacts.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/S0006-3495(78)85487-3</identifier><identifier>PMID: 656543</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Line ; Cell Nucleus - metabolism ; Cell Nucleus - radiation effects ; DNA Repair ; DNA Replication ; Humans ; Kinetics ; Pyrimidine Dimers - metabolism ; Pyrimidine Dimers - radiation effects ; Ultraviolet Rays</subject><ispartof>Biophysical journal, 1978-05, Vol.22 (2), p.249-264</ispartof><rights>1978 The Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-547c12fb63cb7fbe918bf5beb941ed206d77e72e554e83af32306918d2c80b023</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1473442/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006349578854873$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3536,27903,27904,53769,53771,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/656543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ehmann, U.K.</creatorcontrib><creatorcontrib>Cook, K.H.</creatorcontrib><creatorcontrib>Friedberg, E.C.</creatorcontrib><title>The kinetics of thymine dimer excision in ultraviolet-irradiated human cells</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>We have investigated the kinetics of the loss of thymine dimers from the acid-insoluble fraction of several ultraviolet (UV)-irradiated cultured human cell lines. Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21–85 x 10(5) thymine dimers per cell and an eventual maximal loss per cell of 12–20 x 10(5) thymine dimers. The time for half-maximal loss of dimers ranged from 12–22 h after UV irradiation. In contrast, the time for half-maximal repair synthesis of DNA measured by autoradiography was 4.5 h. This figure agrees well with reported half-maximal repair synthesis times, which range from 0.5 to 3.6 h based on our analysis. The discrepancy in the kinetics of the loss of thymine dimers from DNA and repair synthesis is discussed in terms of possible molecular mechanisms of thymine dimer excision in vivo and in terms of possible experimental artifacts.</description><subject>Cell Line</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - radiation effects</subject><subject>DNA Repair</subject><subject>DNA Replication</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Pyrimidine Dimers - metabolism</subject><subject>Pyrimidine Dimers - radiation effects</subject><subject>Ultraviolet Rays</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQtSraspT-gyL5hOCQdvydvYBQ1UKllXpoOVuOPekakrjYyYr-e7LdagUnTqPRe_Nm5j1C3jE4Z8D0xR0A6ErIpfpg6o-1krWpxAFZMCV5BVDrV2Sxp7wmb0r5AcC4AnZMjrTSSooFWd2vkf6MA47RF5paOq6f-rmlIfaYKf72scQ00DjQqRuz28TU4VjFnF2IbsRA11PvBuqx68pbcti6ruDpSz0h36-v7i-_VavbrzeXX1aVl5qNlZLGM942WvjGtA0uWd20qsFmKRkGDjoYg4ajUhJr4VrBBeiZFLivoQEuTsinne7j1PQYPA7zZZ19zLF3-ckmF-2_yBDX9iFtLJNGSLkVeP8ikNOvCcto-1i2L7gB01SskQCcg5mJakf0OZWSsd0vYWC3KdjnFOzWYmtq-5yCFfPc2d8X7qd2ts_w5x2Ms0ubiNkWH3HwGGJGP9qQ4n8W_AGVCZjc</recordid><startdate>19780501</startdate><enddate>19780501</enddate><creator>Ehmann, U.K.</creator><creator>Cook, K.H.</creator><creator>Friedberg, E.C.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19780501</creationdate><title>The kinetics of thymine dimer excision in ultraviolet-irradiated human cells</title><author>Ehmann, U.K. ; Cook, K.H. ; Friedberg, E.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-547c12fb63cb7fbe918bf5beb941ed206d77e72e554e83af32306918d2c80b023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Cell Line</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - radiation effects</topic><topic>DNA Repair</topic><topic>DNA Replication</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Pyrimidine Dimers - metabolism</topic><topic>Pyrimidine Dimers - radiation effects</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ehmann, U.K.</creatorcontrib><creatorcontrib>Cook, K.H.</creatorcontrib><creatorcontrib>Friedberg, E.C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ehmann, U.K.</au><au>Cook, K.H.</au><au>Friedberg, E.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The kinetics of thymine dimer excision in ultraviolet-irradiated human cells</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>1978-05-01</date><risdate>1978</risdate><volume>22</volume><issue>2</issue><spage>249</spage><epage>264</epage><pages>249-264</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>We have investigated the kinetics of the loss of thymine dimers from the acid-insoluble fraction of several ultraviolet (UV)-irradiated cultured human cell lines. Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21–85 x 10(5) thymine dimers per cell and an eventual maximal loss per cell of 12–20 x 10(5) thymine dimers. The time for half-maximal loss of dimers ranged from 12–22 h after UV irradiation. In contrast, the time for half-maximal repair synthesis of DNA measured by autoradiography was 4.5 h. This figure agrees well with reported half-maximal repair synthesis times, which range from 0.5 to 3.6 h based on our analysis. The discrepancy in the kinetics of the loss of thymine dimers from DNA and repair synthesis is discussed in terms of possible molecular mechanisms of thymine dimer excision in vivo and in terms of possible experimental artifacts.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>656543</pmid><doi>10.1016/S0006-3495(78)85487-3</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biophysical journal, 1978-05, Vol.22 (2), p.249-264 |
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| language | eng |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cell Line Cell Nucleus - metabolism Cell Nucleus - radiation effects DNA Repair DNA Replication Humans Kinetics Pyrimidine Dimers - metabolism Pyrimidine Dimers - radiation effects Ultraviolet Rays |
| title | The kinetics of thymine dimer excision in ultraviolet-irradiated human cells |
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