High and low affinity Ca2+ binding to the sarcoplasmic reticulum: use of a high-affinity fluorescent calcium indicator

The fluorescent calcium indicator, calcein, has been used as a high-affinity indicator of Ca2+ in the aqueous phase at physiological pH in the study of high-affinity calcium binding to sarcoplasmic reticulum (SR). The binding constant of the indicator at physiological pH is 10(3)-10(4) M-1 and incre...

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Veröffentlicht in:	Biophysical journal 1977-04, Vol.18 (1), p.3-22
Hauptverfasser:	Chiu, V.C., Haynes, D.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - metabolism Anilino Naphthalenesulfonates Animals Biological Transport, Active Calcium - metabolism Cell-Free System Fluorescent Dyes - metabolism Hydrogen-Ion Concentration Kinetics Ligands Magnesium - metabolism Membrane Proteins - metabolism Models, Biological Muscle Proteins - metabolism Rabbits Sarcoplasmic Reticulum - metabolism Spectrophotometry, Ultraviolet Xanthenes
Online-Zugang:	Volltext
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