Electrospray ionization mass spectrometric characterization of photocrosslinked DNA-EcoRI DNA methyltransferase complexes

We describe a novel strategy combining photocrosslinking and HPLC-based electrospray ionization mass spectrometry to identify UV crosslinked DNA-protein complexes. EcoRI DNA methyltransferase modifies the second adenine within the recognition sequence GAATTC. Substitution of 5-iodouracil for the thy...

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Veröffentlicht in:	Nucleic acids research 1998-01, Vol.26 (2), p.645-649
Hauptverfasser:	Wong, David L., Pavlovich, James G., Reich, Norbert O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Binding Sites Chromatography, High Pressure Liquid Cross-Linking Reagents DNA - chemistry DNA - metabolism Electrophoresis, Polyacrylamide Gel Mass Spectrometry - methods Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - metabolism Photochemistry Site-Specific DNA-Methyltransferase (Adenine-Specific) - chemistry Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism Substrate Specificity Ultraviolet Rays Uracil - analogs & derivatives Uracil - chemistry
Online-Zugang:	Volltext
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