Binding of double-stranded DNA by Escherichia coli RecA protein monitored by a fluorescent dye displacement assay

We have developed a new assay to characterize the double-stranded DNA (dsDNA) binding properties of RecA protein. This assay is based on measurement of changes in the fluorescence of a 4′,6-diamidino-2-phenylindole (DAPI)-dsDNA complex upon RecA protein binding. The binding of RecA protein to a comp...

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Veröffentlicht in:	Nucleic acids research 1998-01, Vol.26 (2), p.650-654
Hauptverfasser:	Zaitsev, Eugene N., Kowalczykowski, Stephen C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - pharmacology Bacteriophages - genetics Binding Sites Binding, Competitive Deoxyadenine Nucleotides - pharmacology DNA - metabolism DNA, Viral - metabolism Drug Stability Escherichia coli - chemistry Fluorescent Dyes Hydrogen-Ion Concentration Indoles - metabolism Kinetics Mutation Osmolar Concentration Rec A Recombinases - chemistry Rec A Recombinases - genetics Rec A Recombinases - metabolism Sodium Chloride Spectrometry, Fluorescence
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