An efficient and economic site-specific deuteration strategy for NMR studies of homologous oligonucleotide repeat sequences

We describe herein the use of a 2H-labeling strategy to achieve specific assignments of considerably overlapped cross peaks in the 1H-NMR spectrum of a DNA trinucleotide repeat sequence. Our strategy focuses on site-specific 2H-labeling of base moieties to simplify the NMR spectral regions which con...

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Veröffentlicht in:	Nucleic acids research 1997-12, Vol.25 (23), p.4758-4763
Hauptverfasser:	Huang, Xuening, Yu, Peilin, LeProust, Eric, Gao, Xiaolian
Format:	Artikel
Sprache:	eng
Schlagworte:	Deuterium DNA - chemistry Magnetic Resonance Spectroscopy - methods Models, Chemical Oligonucleotides - chemistry Protons Repetitive Sequences, Nucleic Acid RNA - chemistry
Online-Zugang:	Volltext
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