Cre-mediated gene deletion in the mammary gland
To delete genes specifically from mammary tissue using the Cre-lox system, we have established transgenic mice expressing Cre recombinase under control of the WAP gene promoter and the MMTV LTR. Cre activity in these mice was evaluated by three criteria. First, the tissue distribution of Cre mRNA wa...
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| Veröffentlicht in: | Nucleic acids research 1997-11, Vol.25 (21), p.4323-4330 |
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| creator | Wagner, Kay-Uwe Wall, Robert J. St-Onge, Luc Gruss, Peter Wynshaw-Boris, Anthony Garrett, Lisa Li, Minglin Furth, Priscilla A. Hennighausen, Lothar |
| description | To delete genes specifically from mammary tissue using the Cre-lox system, we have established transgenic mice expressing Cre recombinase under control of the WAP gene promoter and the MMTV LTR. Cre activity in these mice was evaluated by three criteria. First, the tissue distribution of Cre mRNA was analyzed. Second, an adenovirus carrying a reporter gene was used to determine expression at the level of single cells. Third, tissue specificity of Cre activity was determined in a mouse strain carrying a reporter gene. In adult MMTV-Cre mice expression of the transgene was confined to striated ductal cells of the salivary gland and mammary epithelial cells in virgin and lactating mice. Expression of WAP-Cre was only detected in alveolar epithelial cells of mammary tissue during lactation. Analysis of transgenic mice carrying both the MMTV-Cre and the reporter transgenes revealed recombination in every tissue. In contrast, recombination mediated by Cre under control of the WAP gene promoter was largely restricted to the mammary gland but occasionally observed in the brain. These results show that transgenic mice with WAP-Cre but not MMTV-Cre can be used as a powerful tool to study gene function in development and tumorigenesis in the mammary gland. |
| doi_str_mv | 10.1093/nar/25.21.4323 |
| format | Article |
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Cre activity in these mice was evaluated by three criteria. First, the tissue distribution of Cre mRNA was analyzed. Second, an adenovirus carrying a reporter gene was used to determine expression at the level of single cells. Third, tissue specificity of Cre activity was determined in a mouse strain carrying a reporter gene. In adult MMTV-Cre mice expression of the transgene was confined to striated ductal cells of the salivary gland and mammary epithelial cells in virgin and lactating mice. Expression of WAP-Cre was only detected in alveolar epithelial cells of mammary tissue during lactation. Analysis of transgenic mice carrying both the MMTV-Cre and the reporter transgenes revealed recombination in every tissue. In contrast, recombination mediated by Cre under control of the WAP gene promoter was largely restricted to the mammary gland but occasionally observed in the brain. These results show that transgenic mice with WAP-Cre but not MMTV-Cre can be used as a powerful tool to study gene function in development and tumorigenesis in the mammary gland.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/25.21.4323</identifier><identifier>PMID: 9336464</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenoviridae - genetics ; Animals ; Epithelial Cells ; Female ; Gene Deletion ; Gene Expression Regulation, Developmental ; Integrases - metabolism ; Mammary Glands, Animal - enzymology ; Mammary Glands, Animal - physiology ; Mammary Tumor Virus, Mouse - genetics ; Mice ; Mice, Transgenic ; Milk Proteins - genetics ; Organ Specificity ; Promoter Regions, Genetic - genetics ; Recombination, Genetic ; Repetitive Sequences, Nucleic Acid - genetics ; Transgenes - genetics ; Viral Proteins</subject><ispartof>Nucleic acids research, 1997-11, Vol.25 (21), p.4323-4330</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-3d2d45ba52d9f26c3968fc3572ff1dcc5fde3aef05ad1b3c19e1700667458d753</citedby><cites>FETCH-LOGICAL-c453t-3d2d45ba52d9f26c3968fc3572ff1dcc5fde3aef05ad1b3c19e1700667458d753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC147032/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC147032/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9336464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wagner, Kay-Uwe</creatorcontrib><creatorcontrib>Wall, Robert J.</creatorcontrib><creatorcontrib>St-Onge, Luc</creatorcontrib><creatorcontrib>Gruss, Peter</creatorcontrib><creatorcontrib>Wynshaw-Boris, Anthony</creatorcontrib><creatorcontrib>Garrett, Lisa</creatorcontrib><creatorcontrib>Li, Minglin</creatorcontrib><creatorcontrib>Furth, Priscilla A.</creatorcontrib><creatorcontrib>Hennighausen, Lothar</creatorcontrib><title>Cre-mediated gene deletion in the mammary gland</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Research</addtitle><description>To delete genes specifically from mammary tissue using the Cre-lox system, we have established transgenic mice expressing Cre recombinase under control of the WAP gene promoter and the MMTV LTR. Cre activity in these mice was evaluated by three criteria. First, the tissue distribution of Cre mRNA was analyzed. Second, an adenovirus carrying a reporter gene was used to determine expression at the level of single cells. Third, tissue specificity of Cre activity was determined in a mouse strain carrying a reporter gene. In adult MMTV-Cre mice expression of the transgene was confined to striated ductal cells of the salivary gland and mammary epithelial cells in virgin and lactating mice. Expression of WAP-Cre was only detected in alveolar epithelial cells of mammary tissue during lactation. Analysis of transgenic mice carrying both the MMTV-Cre and the reporter transgenes revealed recombination in every tissue. In contrast, recombination mediated by Cre under control of the WAP gene promoter was largely restricted to the mammary gland but occasionally observed in the brain. These results show that transgenic mice with WAP-Cre but not MMTV-Cre can be used as a powerful tool to study gene function in development and tumorigenesis in the mammary gland.</description><subject>Adenoviridae - genetics</subject><subject>Animals</subject><subject>Epithelial Cells</subject><subject>Female</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Integrases - metabolism</subject><subject>Mammary Glands, Animal - enzymology</subject><subject>Mammary Glands, Animal - physiology</subject><subject>Mammary Tumor Virus, Mouse - genetics</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Milk Proteins - genetics</subject><subject>Organ Specificity</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombination, Genetic</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Transgenes - genetics</subject><subject>Viral Proteins</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUbtu2zAUJYoGruN27VZAUzfJJC8f1pChVR4OEiMdWiDIQtDkla1Gj4SUg-TvI8OGkUyZ7nDOuTgPQr4zmjGaw7S1YcplxlkmgMMnMmageCpyxT-TMQUqU0bF7As5jvE_pUwwKUZklAMoocSYTIuAaYO-sj36ZIUtJh5r7KuuTao26deYNLZpbHhJVrVt_VdyVNo64rf9nZB_52d_i3l6fXNxWfy6Tp2Q0KfguRdyaSX3ecmVg1zNSgdS87Jk3jlZegSLJZXWsyU4liPTlCqlhZx5LWFCTnZ_HzbLwZ7Dtg-2Ng-h2noxna3Me6St1mbVPRkmNB2KmJCfe33oHjcYe9NU0WE9ZMBuE43OYciv6IdEpriU-WzrKNsRXehiDFgezDBqtlOYYQrDpeHMbKcYBD_eRjjQ990PeLrDq9jj8wG24d4oDVqa-e2d-bO4Oi10sTC_4RWv6pQe</recordid><startdate>199711</startdate><enddate>199711</enddate><creator>Wagner, Kay-Uwe</creator><creator>Wall, Robert J.</creator><creator>St-Onge, Luc</creator><creator>Gruss, Peter</creator><creator>Wynshaw-Boris, Anthony</creator><creator>Garrett, Lisa</creator><creator>Li, Minglin</creator><creator>Furth, Priscilla A.</creator><creator>Hennighausen, Lothar</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199711</creationdate><title>Cre-mediated gene deletion in the mammary gland</title><author>Wagner, Kay-Uwe ; Wall, Robert J. ; St-Onge, Luc ; Gruss, Peter ; Wynshaw-Boris, Anthony ; Garrett, Lisa ; Li, Minglin ; Furth, Priscilla A. ; Hennighausen, Lothar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-3d2d45ba52d9f26c3968fc3572ff1dcc5fde3aef05ad1b3c19e1700667458d753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adenoviridae - genetics</topic><topic>Animals</topic><topic>Epithelial Cells</topic><topic>Female</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Integrases - metabolism</topic><topic>Mammary Glands, Animal - enzymology</topic><topic>Mammary Glands, Animal - physiology</topic><topic>Mammary Tumor Virus, Mouse - genetics</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Milk Proteins - genetics</topic><topic>Organ Specificity</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombination, Genetic</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Transgenes - genetics</topic><topic>Viral Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wagner, Kay-Uwe</creatorcontrib><creatorcontrib>Wall, Robert J.</creatorcontrib><creatorcontrib>St-Onge, Luc</creatorcontrib><creatorcontrib>Gruss, Peter</creatorcontrib><creatorcontrib>Wynshaw-Boris, Anthony</creatorcontrib><creatorcontrib>Garrett, Lisa</creatorcontrib><creatorcontrib>Li, Minglin</creatorcontrib><creatorcontrib>Furth, Priscilla A.</creatorcontrib><creatorcontrib>Hennighausen, Lothar</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wagner, Kay-Uwe</au><au>Wall, Robert J.</au><au>St-Onge, Luc</au><au>Gruss, Peter</au><au>Wynshaw-Boris, Anthony</au><au>Garrett, Lisa</au><au>Li, Minglin</au><au>Furth, Priscilla A.</au><au>Hennighausen, Lothar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cre-mediated gene deletion in the mammary gland</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Research</addtitle><date>1997-11</date><risdate>1997</risdate><volume>25</volume><issue>21</issue><spage>4323</spage><epage>4330</epage><pages>4323-4330</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>To delete genes specifically from mammary tissue using the Cre-lox system, we have established transgenic mice expressing Cre recombinase under control of the WAP gene promoter and the MMTV LTR. Cre activity in these mice was evaluated by three criteria. First, the tissue distribution of Cre mRNA was analyzed. Second, an adenovirus carrying a reporter gene was used to determine expression at the level of single cells. Third, tissue specificity of Cre activity was determined in a mouse strain carrying a reporter gene. In adult MMTV-Cre mice expression of the transgene was confined to striated ductal cells of the salivary gland and mammary epithelial cells in virgin and lactating mice. Expression of WAP-Cre was only detected in alveolar epithelial cells of mammary tissue during lactation. Analysis of transgenic mice carrying both the MMTV-Cre and the reporter transgenes revealed recombination in every tissue. In contrast, recombination mediated by Cre under control of the WAP gene promoter was largely restricted to the mammary gland but occasionally observed in the brain. 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| source | MEDLINE; Oxford Journals Open Access Collection; Oxford University Press Journals All Titles (1996-Current); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adenoviridae - genetics Animals Epithelial Cells Female Gene Deletion Gene Expression Regulation, Developmental Integrases - metabolism Mammary Glands, Animal - enzymology Mammary Glands, Animal - physiology Mammary Tumor Virus, Mouse - genetics Mice Mice, Transgenic Milk Proteins - genetics Organ Specificity Promoter Regions, Genetic - genetics Recombination, Genetic Repetitive Sequences, Nucleic Acid - genetics Transgenes - genetics Viral Proteins |
| title | Cre-mediated gene deletion in the mammary gland |
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