An ELISA for Detection of Apoptosis

An ELISA for Detection of Apoptosis

We describe a simple and convenient enzyme-linked immunosorbent assay (ELISA) for the detection of apoptosis in tissue culture. An early event in apoptosis is DNA fragmentation followed by release of nucleosomes into the cytoplasm. Our sandwich assay uses a pair of monoclonal antibodies specific for...

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Veröffentlicht in:Nucleic acids research 1997-02, Vol.25 (3), p.680-681
Hauptverfasser: Salgame, Padmini, Primiano, Laura L., Fincke, John E., Muller, Sylviane, Monestier, Marc
Format: Artikel
Sprache:eng
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Apoptosis - physiology
Enzyme-Linked Immunosorbent Assay - methods
Humans
Jurkat Cells
Nucleosomes
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container_issue 3
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container_title Nucleic acids research
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creator Salgame, Padmini
Primiano, Laura L.
Fincke, John E.
Muller, Sylviane
Monestier, Marc
description We describe a simple and convenient enzyme-linked immunosorbent assay (ELISA) for the detection of apoptosis in tissue culture. An early event in apoptosis is DNA fragmentation followed by release of nucleosomes into the cytoplasm. Our sandwich assay uses a pair of monoclonal antibodies specific for two nucleosomal epitopes to capture and detect cytoplasmic nucleosomes onto the ELISA plate. Our assay is about 500 times more sensitive than the detection of apoptotic DNA ladder by agarose electrophoresis and is especially suited for the testing of large numbers of samples.
doi_str_mv 10.1093/nar/25.3.680
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subjects Apoptosis - physiology
Enzyme-Linked Immunosorbent Assay - methods
Humans
Jurkat Cells
Nucleosomes
title An ELISA for Detection of Apoptosis
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