Genetic Linkage Analysis of the Lesser Grain Borer Rhyzopertha dominica Identifies Two Loci That Confer High-Level Resistance to the Fumigant Phosphine
High levels of inheritable resistance to phosphine in Rhyzopertha dominica have recently been detected in Australia and in an effort to isolate the genes responsible for resistance we have used random amplified DNA fingerprinting (RAF) to produce a genetic linkage map of R. dominica. The map consist...
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| Veröffentlicht in: | Genetics (Austin) 2002-06, Vol.161 (2), p.773-782 |
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| creator | Schlipalius, David I Cheng, Qiang Reilly, Paul E. B Collins, Patrick J Ebert, Paul R |
| description | High levels of inheritable resistance to phosphine in Rhyzopertha dominica have recently been detected in Australia and in an effort to isolate the genes responsible for resistance we have used random amplified DNA fingerprinting (RAF) to produce a genetic linkage map of R. dominica. The map consists of 94 dominant DNA markers with an average distance between markers of 4.6 cM and defines nine linkage groups with a total recombination distance of 390.1 cM. We have identified two loci that are responsible for high-level resistance. One provides approximately 50x resistance to phosphine while the other provides 12.5x resistance and in combination, the two genes act synergistically to provide a resistance level 250x greater than that of fully susceptible beetles. The haploid genome size has been determined to be 4.76 x 10(8) bp, resulting in an average physical distance of 1.2 Mbp per map unit. No recombination has been observed between either of the two resistance loci and their adjacent DNA markers in a population of 44 fully resistant F5 individuals, which indicates that the genes are likely to reside within 0.91 cM (1.1 Mbp) of the DNA markers. |
| doi_str_mv | 10.1093/genetics/161.2.773 |
| format | Article |
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The haploid genome size has been determined to be 4.76 x 10(8) bp, resulting in an average physical distance of 1.2 Mbp per map unit. No recombination has been observed between either of the two resistance loci and their adjacent DNA markers in a population of 44 fully resistant F5 individuals, which indicates that the genes are likely to reside within 0.91 cM (1.1 Mbp) of the DNA markers.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/161.2.773</identifier><identifier>PMID: 12072472</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>Animals ; Bostrichidae ; Chromosome Mapping ; Coleoptera - genetics ; Crosses, Genetic ; Deoxyribonucleic acid ; DNA ; DNA Fingerprinting ; Flow Cytometry ; Genes ; Genetic Markers ; Genetics ; Genome ; Insecticide Resistance - genetics ; Insecticides ; phosphine ; Phosphines ; Random Amplified Polymorphic DNA Technique ; Rhyzopertha dominica</subject><ispartof>Genetics (Austin), 2002-06, Vol.161 (2), p.773-782</ispartof><rights>Copyright Genetics Society of America Jun 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-1ef212d43caf4be58dbd1540ff32c57d2acee7faa2ac8f6083e580be8276ec823</citedby><cites>FETCH-LOGICAL-c487t-1ef212d43caf4be58dbd1540ff32c57d2acee7faa2ac8f6083e580be8276ec823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12072472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schlipalius, David I</creatorcontrib><creatorcontrib>Cheng, Qiang</creatorcontrib><creatorcontrib>Reilly, Paul E. B</creatorcontrib><creatorcontrib>Collins, Patrick J</creatorcontrib><creatorcontrib>Ebert, Paul R</creatorcontrib><title>Genetic Linkage Analysis of the Lesser Grain Borer Rhyzopertha dominica Identifies Two Loci That Confer High-Level Resistance to the Fumigant Phosphine</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>High levels of inheritable resistance to phosphine in Rhyzopertha dominica have recently been detected in Australia and in an effort to isolate the genes responsible for resistance we have used random amplified DNA fingerprinting (RAF) to produce a genetic linkage map of R. dominica. The map consists of 94 dominant DNA markers with an average distance between markers of 4.6 cM and defines nine linkage groups with a total recombination distance of 390.1 cM. We have identified two loci that are responsible for high-level resistance. One provides approximately 50x resistance to phosphine while the other provides 12.5x resistance and in combination, the two genes act synergistically to provide a resistance level 250x greater than that of fully susceptible beetles. The haploid genome size has been determined to be 4.76 x 10(8) bp, resulting in an average physical distance of 1.2 Mbp per map unit. No recombination has been observed between either of the two resistance loci and their adjacent DNA markers in a population of 44 fully resistant F5 individuals, which indicates that the genes are likely to reside within 0.91 cM (1.1 Mbp) of the DNA markers.</description><subject>Animals</subject><subject>Bostrichidae</subject><subject>Chromosome Mapping</subject><subject>Coleoptera - genetics</subject><subject>Crosses, Genetic</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Fingerprinting</subject><subject>Flow Cytometry</subject><subject>Genes</subject><subject>Genetic Markers</subject><subject>Genetics</subject><subject>Genome</subject><subject>Insecticide Resistance - genetics</subject><subject>Insecticides</subject><subject>phosphine</subject><subject>Phosphines</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Rhyzopertha dominica</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1uEzEUhS0EoiHwAiyQxYLdpP6Zv2yQ2oimlUYCVWFtOZ7rGZcZO9ieRuFFeF0MCQRY-Uo-5_hcfwi9pmRByZJfdmAhGhUuaUkXbFFV_Ama0WXOM1Zy-hTNCKFlVlacXqAXITwQQsplUT9HF5SRiuUVm6Hv62MIboz9IjvAV1YOh2ACdhrHHnADIYDHay-NxdfOp_m-P3xzO_Cxl7h1o7FGSXzXgo1GGwh4s3e4ccrgTS8jXjmrk-nWdH3WwCMM-B5SfpRWAY7u1yM302g6aSP-1Luw642Fl-iZlkOAV6dzjj7ffNisbrPm4_puddVkKq-rmFHQjLI250rqfAtF3W5bWuREa85UUbVMKoBKS5mGWpek5klDtlCzqgRVMz5H74-5u2k7QqvSEl4OYufNKP1BOGnEvzfW9KJzj4LmJaPFMgW8OwV493WCEMVogoJhkBbcFAStOSdFWSbh2_-ED27y6beDYDSnLKep3Ryxo0h5F4IH_acJJeIndPEbukjQBRMJejK9-XuHs-VE-dyxTxD2xoMIoxyGJKdiv9-fk34AUEi7eg</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Schlipalius, David I</creator><creator>Cheng, Qiang</creator><creator>Reilly, Paul E. 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B</au><au>Collins, Patrick J</au><au>Ebert, Paul R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Linkage Analysis of the Lesser Grain Borer Rhyzopertha dominica Identifies Two Loci That Confer High-Level Resistance to the Fumigant Phosphine</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>161</volume><issue>2</issue><spage>773</spage><epage>782</epage><pages>773-782</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>High levels of inheritable resistance to phosphine in Rhyzopertha dominica have recently been detected in Australia and in an effort to isolate the genes responsible for resistance we have used random amplified DNA fingerprinting (RAF) to produce a genetic linkage map of R. dominica. The map consists of 94 dominant DNA markers with an average distance between markers of 4.6 cM and defines nine linkage groups with a total recombination distance of 390.1 cM. We have identified two loci that are responsible for high-level resistance. One provides approximately 50x resistance to phosphine while the other provides 12.5x resistance and in combination, the two genes act synergistically to provide a resistance level 250x greater than that of fully susceptible beetles. The haploid genome size has been determined to be 4.76 x 10(8) bp, resulting in an average physical distance of 1.2 Mbp per map unit. 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| ispartof | Genetics (Austin), 2002-06, Vol.161 (2), p.773-782 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Bostrichidae Chromosome Mapping Coleoptera - genetics Crosses, Genetic Deoxyribonucleic acid DNA DNA Fingerprinting Flow Cytometry Genes Genetic Markers Genetics Genome Insecticide Resistance - genetics Insecticides phosphine Phosphines Random Amplified Polymorphic DNA Technique Rhyzopertha dominica |
| title | Genetic Linkage Analysis of the Lesser Grain Borer Rhyzopertha dominica Identifies Two Loci That Confer High-Level Resistance to the Fumigant Phosphine |
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