Genetic Analysis Reveals That FLO11 Upregulation and Cell Polarization Independently Regulate Invasive Growth in Saccharomyces cerevisiae
Under inducing conditions, haploid Saccharomyces cerevisiae perform a dimorphic transition from yeast-form growth on the agar surface to invasive growth, where chains of cells dig into the solid growth medium. Previous work on signaling cascades that promote agar invasion has demonstrated upregulati...
Gespeichert in:
| Veröffentlicht in: | Genetics (Austin) 2000-11, Vol.156 (3), p.1005-1023 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1023 |
|---|---|
| container_issue | 3 |
| container_start_page | 1005 |
| container_title | Genetics (Austin) |
| container_volume | 156 |
| creator | Palecek, Sean P Parikh, Archita S Kron, Stephen J |
| description | Under inducing conditions, haploid Saccharomyces cerevisiae perform a dimorphic transition from yeast-form growth on the agar surface to invasive growth, where chains of cells dig into the solid growth medium. Previous work on signaling cascades that promote agar invasion has demonstrated upregulation of FLO11, a cell-surface flocculin involved in cell-cell adhesion. We find that increasing FLO11 transcription is sufficient to induce both invasive and filamentous growth. A genetic screen for repressors of FLO11 isolated mutant strains that dig into agar (dia) and identified mutations in 35 different genes: ELM1, HSL1, HSL7, BUD3, BUD4, BUD10, AXL1, SIR2, SIR4, BEM2, PGI1, GND1, YDJ1, ARO7, GRR1, CDC53, HSC82, ZUO1, ADH1, CSE2, GCR1, IRA1, MSN5, SRB8, SSN3, SSN8, BPL1, GTR1, MED1, SKN7, TAF25, DIA1, DIA2, DIA3, and DIA4. Indeed, agar invasion in 20 dia mutants requires upregulation of the endogenous FLO11 promoter. However, 13 mutants promote agar invasion even with FLO11 clamped at a constitutive low-expression level. These FLO11 promoter-independent dia mutants establish distinct invasive growth pathways due to polarized bud site selection and/or cell elongation. Epistasis with the STE MAP kinase cascade and cytokinesis/budding checkpoint shows these pathways are targets of DIA genes that repress agar invasion by FLO11 promoter-dependent and -independent mechanisms, respectively. |
| doi_str_mv | 10.1093/genetics/156.3.1005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1461303</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>64855181</sourcerecordid><originalsourceid>FETCH-LOGICAL-c491t-7fd135483466070a20cff53a6ea038b5d4523631a9a046ddf2a1e0875543e66c3</originalsourceid><addsrcrecordid>eNpdkd1u1DAQhS0EotuFJ0BCFhf0Kq0nTpzkBqla0aXSSkXQXltTZ7JxlU0WOz9a3oC3xiULFG5safzN8cw5jL0BcQ6ikBdbaqm3xl9Aqs5lqIn0GVtAkcgoVhKes4UQoCKVSThhp94_CCFUkeYv2QmAUFLlsGA_1rMKv2yxOXjr-RcaCRvPb2vs-dXmBoDf7R1thwZ727Uc25KvqGn4565BZ7_P1eu2pD2Fo-2bQ9D4hVMoj-jtSHztuqmvuW35VzSmRtftDoY8N-RotN4ivWIvqvAvvT7eS3Z39fF29Sna3KyvV5ebyCQF9FFWlSDTJJeJUiITGAtTValERShkfp-WSRrLsD4WKBJVllWMQCLP0jSRpJSRS_Zh1t0P9zsqTZjYYaP3zu7QHXSHVv_70tpab7tRQ6JAChkE3h8FXPdtIN_rnfUmOIItdYPXkGVSxcH2JXv3H_jQDS747HUMCcQiF2mA5AwZ13nvqPozCQj9mLP-nbMOOWupH3MOXW-fLvG35xhsAM5moLbberKOtN9h0wQc9DRNT6R-Aq4utbg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>214120805</pqid></control><display><type>article</type><title>Genetic Analysis Reveals That FLO11 Upregulation and Cell Polarization Independently Regulate Invasive Growth in Saccharomyces cerevisiae</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Palecek, Sean P ; Parikh, Archita S ; Kron, Stephen J</creator><creatorcontrib>Palecek, Sean P ; Parikh, Archita S ; Kron, Stephen J</creatorcontrib><description>Under inducing conditions, haploid Saccharomyces cerevisiae perform a dimorphic transition from yeast-form growth on the agar surface to invasive growth, where chains of cells dig into the solid growth medium. Previous work on signaling cascades that promote agar invasion has demonstrated upregulation of FLO11, a cell-surface flocculin involved in cell-cell adhesion. We find that increasing FLO11 transcription is sufficient to induce both invasive and filamentous growth. A genetic screen for repressors of FLO11 isolated mutant strains that dig into agar (dia) and identified mutations in 35 different genes: ELM1, HSL1, HSL7, BUD3, BUD4, BUD10, AXL1, SIR2, SIR4, BEM2, PGI1, GND1, YDJ1, ARO7, GRR1, CDC53, HSC82, ZUO1, ADH1, CSE2, GCR1, IRA1, MSN5, SRB8, SSN3, SSN8, BPL1, GTR1, MED1, SKN7, TAF25, DIA1, DIA2, DIA3, and DIA4. Indeed, agar invasion in 20 dia mutants requires upregulation of the endogenous FLO11 promoter. However, 13 mutants promote agar invasion even with FLO11 clamped at a constitutive low-expression level. These FLO11 promoter-independent dia mutants establish distinct invasive growth pathways due to polarized bud site selection and/or cell elongation. Epistasis with the STE MAP kinase cascade and cytokinesis/budding checkpoint shows these pathways are targets of DIA genes that repress agar invasion by FLO11 promoter-dependent and -independent mechanisms, respectively.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/156.3.1005</identifier><identifier>PMID: 11063681</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>ADH1 gene ; Analysis ; ARO7 gene ; AXL1 gene ; Base Sequence ; BEM2 gene ; BPL1 gene ; BUD10 gene ; BUD3 gene ; BUD4 gene ; CDC53 gene ; Cell Division - genetics ; Cell Polarity ; Cellular biology ; CSE2 gene ; DIA1 gene ; ELM1 gene ; FLO11 gene ; flo11 protein ; Flowers & plants ; Fungal Proteins - genetics ; GCR1 gene ; Gene Expression Regulation, Fungal ; Genes, Fungal ; Genetics ; Genotype ; GND1 gene ; GRR1 gene ; GTR1 gene ; HSC82 gene ; HSL1 gene ; HSL7 gene ; IRA1 gene ; MED1 gene ; Membrane Glycoproteins ; Membrane Proteins - genetics ; Membrane Proteins - physiology ; Molecular Sequence Data ; MSN5 gene ; Mutagenesis, Insertional ; Oligodeoxyribonucleotides ; PGI1 gene ; Physical growth ; Promoter Regions, Genetic ; Recombinant Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - physiology ; Saccharomyces cerevisiae Proteins ; Signal Transduction ; SIR2 gene ; SIR4 gene ; SKN7 gene ; SRB8 gene ; SSN3 gene ; SSN8 gene ; TAF25 gene ; YDJ1 gene ; ZU01 gene</subject><ispartof>Genetics (Austin), 2000-11, Vol.156 (3), p.1005-1023</ispartof><rights>Copyright Genetics Society of America Nov 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-7fd135483466070a20cff53a6ea038b5d4523631a9a046ddf2a1e0875543e66c3</citedby><cites>FETCH-LOGICAL-c491t-7fd135483466070a20cff53a6ea038b5d4523631a9a046ddf2a1e0875543e66c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11063681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palecek, Sean P</creatorcontrib><creatorcontrib>Parikh, Archita S</creatorcontrib><creatorcontrib>Kron, Stephen J</creatorcontrib><title>Genetic Analysis Reveals That FLO11 Upregulation and Cell Polarization Independently Regulate Invasive Growth in Saccharomyces cerevisiae</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>Under inducing conditions, haploid Saccharomyces cerevisiae perform a dimorphic transition from yeast-form growth on the agar surface to invasive growth, where chains of cells dig into the solid growth medium. Previous work on signaling cascades that promote agar invasion has demonstrated upregulation of FLO11, a cell-surface flocculin involved in cell-cell adhesion. We find that increasing FLO11 transcription is sufficient to induce both invasive and filamentous growth. A genetic screen for repressors of FLO11 isolated mutant strains that dig into agar (dia) and identified mutations in 35 different genes: ELM1, HSL1, HSL7, BUD3, BUD4, BUD10, AXL1, SIR2, SIR4, BEM2, PGI1, GND1, YDJ1, ARO7, GRR1, CDC53, HSC82, ZUO1, ADH1, CSE2, GCR1, IRA1, MSN5, SRB8, SSN3, SSN8, BPL1, GTR1, MED1, SKN7, TAF25, DIA1, DIA2, DIA3, and DIA4. Indeed, agar invasion in 20 dia mutants requires upregulation of the endogenous FLO11 promoter. However, 13 mutants promote agar invasion even with FLO11 clamped at a constitutive low-expression level. These FLO11 promoter-independent dia mutants establish distinct invasive growth pathways due to polarized bud site selection and/or cell elongation. Epistasis with the STE MAP kinase cascade and cytokinesis/budding checkpoint shows these pathways are targets of DIA genes that repress agar invasion by FLO11 promoter-dependent and -independent mechanisms, respectively.</description><subject>ADH1 gene</subject><subject>Analysis</subject><subject>ARO7 gene</subject><subject>AXL1 gene</subject><subject>Base Sequence</subject><subject>BEM2 gene</subject><subject>BPL1 gene</subject><subject>BUD10 gene</subject><subject>BUD3 gene</subject><subject>BUD4 gene</subject><subject>CDC53 gene</subject><subject>Cell Division - genetics</subject><subject>Cell Polarity</subject><subject>Cellular biology</subject><subject>CSE2 gene</subject><subject>DIA1 gene</subject><subject>ELM1 gene</subject><subject>FLO11 gene</subject><subject>flo11 protein</subject><subject>Flowers & plants</subject><subject>Fungal Proteins - genetics</subject><subject>GCR1 gene</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Genetics</subject><subject>Genotype</subject><subject>GND1 gene</subject><subject>GRR1 gene</subject><subject>GTR1 gene</subject><subject>HSC82 gene</subject><subject>HSL1 gene</subject><subject>HSL7 gene</subject><subject>IRA1 gene</subject><subject>MED1 gene</subject><subject>Membrane Glycoproteins</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - physiology</subject><subject>Molecular Sequence Data</subject><subject>MSN5 gene</subject><subject>Mutagenesis, Insertional</subject><subject>Oligodeoxyribonucleotides</subject><subject>PGI1 gene</subject><subject>Physical growth</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Signal Transduction</subject><subject>SIR2 gene</subject><subject>SIR4 gene</subject><subject>SKN7 gene</subject><subject>SRB8 gene</subject><subject>SSN3 gene</subject><subject>SSN8 gene</subject><subject>TAF25 gene</subject><subject>YDJ1 gene</subject><subject>ZU01 gene</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkd1u1DAQhS0EotuFJ0BCFhf0Kq0nTpzkBqla0aXSSkXQXltTZ7JxlU0WOz9a3oC3xiULFG5safzN8cw5jL0BcQ6ikBdbaqm3xl9Aqs5lqIn0GVtAkcgoVhKes4UQoCKVSThhp94_CCFUkeYv2QmAUFLlsGA_1rMKv2yxOXjr-RcaCRvPb2vs-dXmBoDf7R1thwZ727Uc25KvqGn4565BZ7_P1eu2pD2Fo-2bQ9D4hVMoj-jtSHztuqmvuW35VzSmRtftDoY8N-RotN4ivWIvqvAvvT7eS3Z39fF29Sna3KyvV5ebyCQF9FFWlSDTJJeJUiITGAtTValERShkfp-WSRrLsD4WKBJVllWMQCLP0jSRpJSRS_Zh1t0P9zsqTZjYYaP3zu7QHXSHVv_70tpab7tRQ6JAChkE3h8FXPdtIN_rnfUmOIItdYPXkGVSxcH2JXv3H_jQDS747HUMCcQiF2mA5AwZ13nvqPozCQj9mLP-nbMOOWupH3MOXW-fLvG35xhsAM5moLbberKOtN9h0wQc9DRNT6R-Aq4utbg</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Palecek, Sean P</creator><creator>Parikh, Archita S</creator><creator>Kron, Stephen J</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20001101</creationdate><title>Genetic Analysis Reveals That FLO11 Upregulation and Cell Polarization Independently Regulate Invasive Growth in Saccharomyces cerevisiae</title><author>Palecek, Sean P ; Parikh, Archita S ; Kron, Stephen J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-7fd135483466070a20cff53a6ea038b5d4523631a9a046ddf2a1e0875543e66c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>ADH1 gene</topic><topic>Analysis</topic><topic>ARO7 gene</topic><topic>AXL1 gene</topic><topic>Base Sequence</topic><topic>BEM2 gene</topic><topic>BPL1 gene</topic><topic>BUD10 gene</topic><topic>BUD3 gene</topic><topic>BUD4 gene</topic><topic>CDC53 gene</topic><topic>Cell Division - genetics</topic><topic>Cell Polarity</topic><topic>Cellular biology</topic><topic>CSE2 gene</topic><topic>DIA1 gene</topic><topic>ELM1 gene</topic><topic>FLO11 gene</topic><topic>flo11 protein</topic><topic>Flowers & plants</topic><topic>Fungal Proteins - genetics</topic><topic>GCR1 gene</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Genetics</topic><topic>Genotype</topic><topic>GND1 gene</topic><topic>GRR1 gene</topic><topic>GTR1 gene</topic><topic>HSC82 gene</topic><topic>HSL1 gene</topic><topic>HSL7 gene</topic><topic>IRA1 gene</topic><topic>MED1 gene</topic><topic>Membrane Glycoproteins</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - physiology</topic><topic>Molecular Sequence Data</topic><topic>MSN5 gene</topic><topic>Mutagenesis, Insertional</topic><topic>Oligodeoxyribonucleotides</topic><topic>PGI1 gene</topic><topic>Physical growth</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Signal Transduction</topic><topic>SIR2 gene</topic><topic>SIR4 gene</topic><topic>SKN7 gene</topic><topic>SRB8 gene</topic><topic>SSN3 gene</topic><topic>SSN8 gene</topic><topic>TAF25 gene</topic><topic>YDJ1 gene</topic><topic>ZU01 gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palecek, Sean P</creatorcontrib><creatorcontrib>Parikh, Archita S</creatorcontrib><creatorcontrib>Kron, Stephen J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palecek, Sean P</au><au>Parikh, Archita S</au><au>Kron, Stephen J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Analysis Reveals That FLO11 Upregulation and Cell Polarization Independently Regulate Invasive Growth in Saccharomyces cerevisiae</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>2000-11-01</date><risdate>2000</risdate><volume>156</volume><issue>3</issue><spage>1005</spage><epage>1023</epage><pages>1005-1023</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>Under inducing conditions, haploid Saccharomyces cerevisiae perform a dimorphic transition from yeast-form growth on the agar surface to invasive growth, where chains of cells dig into the solid growth medium. Previous work on signaling cascades that promote agar invasion has demonstrated upregulation of FLO11, a cell-surface flocculin involved in cell-cell adhesion. We find that increasing FLO11 transcription is sufficient to induce both invasive and filamentous growth. A genetic screen for repressors of FLO11 isolated mutant strains that dig into agar (dia) and identified mutations in 35 different genes: ELM1, HSL1, HSL7, BUD3, BUD4, BUD10, AXL1, SIR2, SIR4, BEM2, PGI1, GND1, YDJ1, ARO7, GRR1, CDC53, HSC82, ZUO1, ADH1, CSE2, GCR1, IRA1, MSN5, SRB8, SSN3, SSN8, BPL1, GTR1, MED1, SKN7, TAF25, DIA1, DIA2, DIA3, and DIA4. Indeed, agar invasion in 20 dia mutants requires upregulation of the endogenous FLO11 promoter. However, 13 mutants promote agar invasion even with FLO11 clamped at a constitutive low-expression level. These FLO11 promoter-independent dia mutants establish distinct invasive growth pathways due to polarized bud site selection and/or cell elongation. Epistasis with the STE MAP kinase cascade and cytokinesis/budding checkpoint shows these pathways are targets of DIA genes that repress agar invasion by FLO11 promoter-dependent and -independent mechanisms, respectively.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>11063681</pmid><doi>10.1093/genetics/156.3.1005</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0016-6731 |
| ispartof | Genetics (Austin), 2000-11, Vol.156 (3), p.1005-1023 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1461303 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | ADH1 gene Analysis ARO7 gene AXL1 gene Base Sequence BEM2 gene BPL1 gene BUD10 gene BUD3 gene BUD4 gene CDC53 gene Cell Division - genetics Cell Polarity Cellular biology CSE2 gene DIA1 gene ELM1 gene FLO11 gene flo11 protein Flowers & plants Fungal Proteins - genetics GCR1 gene Gene Expression Regulation, Fungal Genes, Fungal Genetics Genotype GND1 gene GRR1 gene GTR1 gene HSC82 gene HSL1 gene HSL7 gene IRA1 gene MED1 gene Membrane Glycoproteins Membrane Proteins - genetics Membrane Proteins - physiology Molecular Sequence Data MSN5 gene Mutagenesis, Insertional Oligodeoxyribonucleotides PGI1 gene Physical growth Promoter Regions, Genetic Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins Signal Transduction SIR2 gene SIR4 gene SKN7 gene SRB8 gene SSN3 gene SSN8 gene TAF25 gene YDJ1 gene ZU01 gene |
| title | Genetic Analysis Reveals That FLO11 Upregulation and Cell Polarization Independently Regulate Invasive Growth in Saccharomyces cerevisiae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T18%3A48%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20Analysis%20Reveals%20That%20FLO11%20Upregulation%20and%20Cell%20Polarization%20Independently%20Regulate%20Invasive%20Growth%20in%20Saccharomyces%20cerevisiae&rft.jtitle=Genetics%20(Austin)&rft.au=Palecek,%20Sean%20P&rft.date=2000-11-01&rft.volume=156&rft.issue=3&rft.spage=1005&rft.epage=1023&rft.pages=1005-1023&rft.issn=0016-6731&rft.eissn=1943-2631&rft.coden=GENTAE&rft_id=info:doi/10.1093/genetics/156.3.1005&rft_dat=%3Cproquest_pubme%3E64855181%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=214120805&rft_id=info:pmid/11063681&rfr_iscdi=true |