Transposon tagging of the Sulfur gene of tobacco using engineered maize Ac/Ds elements
The Sulfur gene of tobacco is nuclearly encoded. A Su allele at this locus acts as a dominant semilethal mutation and causes reduced accumulation of chlorophyll, resulting in a yellow color in the plant. An engineered transposon tagging system, based upon the maize element Ac/Ds, was used to mutate...
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| Veröffentlicht in: | Genetics (Austin) 1999-12, Vol.153 (4), p.1919-1928 |
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| container_end_page | 1928 |
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| container_issue | 4 |
| container_start_page | 1919 |
| container_title | Genetics (Austin) |
| container_volume | 153 |
| creator | Fitzmaurice, W.P Nguyen, L.V Wernsman, E.A Thompson, W.F Conkling, M.A |
| description | The Sulfur gene of tobacco is nuclearly encoded. A Su allele at this locus acts as a dominant semilethal mutation and causes reduced accumulation of chlorophyll, resulting in a yellow color in the plant. An engineered transposon tagging system, based upon the maize element Ac/Ds, was used to mutate the gene. High frequency of transposon excision from the Su locus produced variegated sectors. Plants regenerated from the variegated sector exhibited a similar variegated phenotype. Genetic analyses showed that the variegation was always associated with the transposase construct and the transposon was linked to the Su locus. Sequences surrounding the transposon were isolated, and five revertant sectors possessed typical direct repeats following Ds excisions. These genetic and molecular data are consistent with the tagging of the Su allele by the transposon. |
| doi_str_mv | 10.1093/genetics/153.4.1919 |
| format | Article |
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A Su allele at this locus acts as a dominant semilethal mutation and causes reduced accumulation of chlorophyll, resulting in a yellow color in the plant. An engineered transposon tagging system, based upon the maize element Ac/Ds, was used to mutate the gene. High frequency of transposon excision from the Su locus produced variegated sectors. Plants regenerated from the variegated sector exhibited a similar variegated phenotype. Genetic analyses showed that the variegation was always associated with the transposase construct and the transposon was linked to the Su locus. Sequences surrounding the transposon were isolated, and five revertant sectors possessed typical direct repeats following Ds excisions. These genetic and molecular data are consistent with the tagging of the Su allele by the transposon.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/153.4.1919</identifier><identifier>PMID: 10581296</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>Base Sequence ; biosynthesis ; chlorophyll ; DNA Transposable Elements ; DNA, Plant ; genbank/af050164 ; gene tagging ; genes ; genetic change ; Genetic Engineering ; genetic excision ; Genetic Linkage ; genetic techniques and protocols ; Iron-Sulfur Proteins - genetics ; leaves ; linkage (genetics) ; loci ; Molecular Sequence Data ; Mutagenesis ; mutants ; Nicotiana - genetics ; Nicotiana tabacum ; nucleotide sequences ; Phenotype ; Plants, Toxic ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; regenerative ability ; restriction fragment length polymorphism ; segregation ; Su gene ; su locus ; Sulfur gene ; transposition ; transposon Ac ; transposon Ds ; transposons ; variegation ; Zea mays ; Zea mays - genetics</subject><ispartof>Genetics (Austin), 1999-12, Vol.153 (4), p.1919-1928</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-a40b15ae35fb33da383d2f71b07bc2d499de325d1a8b46e233a394a6c612bb03</citedby><cites>FETCH-LOGICAL-c488t-a40b15ae35fb33da383d2f71b07bc2d499de325d1a8b46e233a394a6c612bb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10581296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fitzmaurice, W.P</creatorcontrib><creatorcontrib>Nguyen, L.V</creatorcontrib><creatorcontrib>Wernsman, E.A</creatorcontrib><creatorcontrib>Thompson, W.F</creatorcontrib><creatorcontrib>Conkling, M.A</creatorcontrib><title>Transposon tagging of the Sulfur gene of tobacco using engineered maize Ac/Ds elements</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>The Sulfur gene of tobacco is nuclearly encoded. A Su allele at this locus acts as a dominant semilethal mutation and causes reduced accumulation of chlorophyll, resulting in a yellow color in the plant. An engineered transposon tagging system, based upon the maize element Ac/Ds, was used to mutate the gene. High frequency of transposon excision from the Su locus produced variegated sectors. Plants regenerated from the variegated sector exhibited a similar variegated phenotype. Genetic analyses showed that the variegation was always associated with the transposase construct and the transposon was linked to the Su locus. Sequences surrounding the transposon were isolated, and five revertant sectors possessed typical direct repeats following Ds excisions. These genetic and molecular data are consistent with the tagging of the Su allele by the transposon.</description><subject>Base Sequence</subject><subject>biosynthesis</subject><subject>chlorophyll</subject><subject>DNA Transposable Elements</subject><subject>DNA, Plant</subject><subject>genbank/af050164</subject><subject>gene tagging</subject><subject>genes</subject><subject>genetic change</subject><subject>Genetic Engineering</subject><subject>genetic excision</subject><subject>Genetic Linkage</subject><subject>genetic techniques and protocols</subject><subject>Iron-Sulfur Proteins - genetics</subject><subject>leaves</subject><subject>linkage (genetics)</subject><subject>loci</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>mutants</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana tabacum</subject><subject>nucleotide sequences</subject><subject>Phenotype</subject><subject>Plants, Toxic</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>regenerative ability</subject><subject>restriction fragment length polymorphism</subject><subject>segregation</subject><subject>Su gene</subject><subject>su locus</subject><subject>Sulfur gene</subject><subject>transposition</subject><subject>transposon Ac</subject><subject>transposon Ds</subject><subject>transposons</subject><subject>variegation</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9v1DAQxS0EokvhEyBBTvSUXY_tOPEFqSrlj1SJQxeulu1MskZJvNhJV_DpSUhB7Wmkmd-8N5pHyGugW6CK71occPQu7aDgW7EFBeoJ2YASPGeSw1OyoRRkLksOZ-RFSj8opVIV1XNyBrSogCm5Id_30QzpGFIYstG0rR_aLDTZeMDsduqaKWaLzd9WsMa5kE1pYXCYUcSIddYb_xuzS7f7kDLssMdhTC_Js8Z0CV_d13Oy_3i9v_qc33z99OXq8iZ3oqrG3AhqoTDIi8ZyXhte8Zo1JVhaWsdqoVSNnBU1mMoKiYxzw5Uw0klg1lJ-Tt6vssfJ9li72TqaTh-j7038pYPx-vFk8AfdhjsNQtKqgFng3b1ADD8nTKPufXLYdWbAMCUNpWBC0AXkK-hiSCli898EqF7i0P_i0HMcWugljnnrzcP7Huys_5-BixU4-PZw8hF16k3XzTjo0-n0SOrtSjYmaNNGn_S3WzZfRpkSQpWM_wEJkaD0</recordid><startdate>19991201</startdate><enddate>19991201</enddate><creator>Fitzmaurice, W.P</creator><creator>Nguyen, L.V</creator><creator>Wernsman, E.A</creator><creator>Thompson, W.F</creator><creator>Conkling, M.A</creator><general>Genetics Soc America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19991201</creationdate><title>Transposon tagging of the Sulfur gene of tobacco using engineered maize Ac/Ds elements</title><author>Fitzmaurice, W.P ; Nguyen, L.V ; Wernsman, E.A ; Thompson, W.F ; Conkling, M.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-a40b15ae35fb33da383d2f71b07bc2d499de325d1a8b46e233a394a6c612bb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Base Sequence</topic><topic>biosynthesis</topic><topic>chlorophyll</topic><topic>DNA Transposable Elements</topic><topic>DNA, Plant</topic><topic>genbank/af050164</topic><topic>gene tagging</topic><topic>genes</topic><topic>genetic change</topic><topic>Genetic Engineering</topic><topic>genetic excision</topic><topic>Genetic Linkage</topic><topic>genetic techniques and protocols</topic><topic>Iron-Sulfur Proteins - genetics</topic><topic>leaves</topic><topic>linkage (genetics)</topic><topic>loci</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>mutants</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana tabacum</topic><topic>nucleotide sequences</topic><topic>Phenotype</topic><topic>Plants, Toxic</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>regenerative ability</topic><topic>restriction fragment length polymorphism</topic><topic>segregation</topic><topic>Su gene</topic><topic>su locus</topic><topic>Sulfur gene</topic><topic>transposition</topic><topic>transposon Ac</topic><topic>transposon Ds</topic><topic>transposons</topic><topic>variegation</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fitzmaurice, W.P</creatorcontrib><creatorcontrib>Nguyen, L.V</creatorcontrib><creatorcontrib>Wernsman, E.A</creatorcontrib><creatorcontrib>Thompson, W.F</creatorcontrib><creatorcontrib>Conkling, M.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fitzmaurice, W.P</au><au>Nguyen, L.V</au><au>Wernsman, E.A</au><au>Thompson, W.F</au><au>Conkling, M.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transposon tagging of the Sulfur gene of tobacco using engineered maize Ac/Ds elements</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1999-12-01</date><risdate>1999</risdate><volume>153</volume><issue>4</issue><spage>1919</spage><epage>1928</epage><pages>1919-1928</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><abstract>The Sulfur gene of tobacco is nuclearly encoded. A Su allele at this locus acts as a dominant semilethal mutation and causes reduced accumulation of chlorophyll, resulting in a yellow color in the plant. An engineered transposon tagging system, based upon the maize element Ac/Ds, was used to mutate the gene. High frequency of transposon excision from the Su locus produced variegated sectors. Plants regenerated from the variegated sector exhibited a similar variegated phenotype. Genetic analyses showed that the variegation was always associated with the transposase construct and the transposon was linked to the Su locus. Sequences surrounding the transposon were isolated, and five revertant sectors possessed typical direct repeats following Ds excisions. These genetic and molecular data are consistent with the tagging of the Su allele by the transposon.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>10581296</pmid><doi>10.1093/genetics/153.4.1919</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genetics (Austin), 1999-12, Vol.153 (4), p.1919-1928 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1460851 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Base Sequence biosynthesis chlorophyll DNA Transposable Elements DNA, Plant genbank/af050164 gene tagging genes genetic change Genetic Engineering genetic excision Genetic Linkage genetic techniques and protocols Iron-Sulfur Proteins - genetics leaves linkage (genetics) loci Molecular Sequence Data Mutagenesis mutants Nicotiana - genetics Nicotiana tabacum nucleotide sequences Phenotype Plants, Toxic Polymerase Chain Reaction Polymorphism, Restriction Fragment Length regenerative ability restriction fragment length polymorphism segregation Su gene su locus Sulfur gene transposition transposon Ac transposon Ds transposons variegation Zea mays Zea mays - genetics |
| title | Transposon tagging of the Sulfur gene of tobacco using engineered maize Ac/Ds elements |
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