Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster
Alternatively spliced Ultrabithorax mRNAs differ by the presence of internal exons mI and mII. Two approaches were used to identify trans-acting factors required for inclusion of these cassette exons. First, mutations in a set of genes implicated in the control of other alternative splicing decision...
Gespeichert in:
Veröffentlicht in: | Genetics (Austin) 1999-04, Vol.151 (4), p.1517-1529 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1529 |
---|---|
container_issue | 4 |
container_start_page | 1517 |
container_title | Genetics (Austin) |
container_volume | 151 |
creator | Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.) Hatton, A.R Lopez, A.J |
description | Alternatively spliced Ultrabithorax mRNAs differ by the presence of internal exons mI and mII. Two approaches were used to identify trans-acting factors required for inclusion of these cassette exons. First, mutations in a set of genes implicated in the control of other alternative splicing decisions were tested for dominant effects on the Ubx alternative splicing pattern. To identify additional genes involved in regulation of Ubx splicing, a large collection of deficiencies was tested first for dominant enhancement of the haploinsufficient Ubx haltere phenotype and second for effects on the splicing pattern. Inclusion of the cassette exons in Ubx mRNAs was reduced strongly in heterozygotes for hypomorphic alleles of hrp48, which encodes a member of the hnRNP A/B family and is implicated in control of P-element splicing. Significant reductions of mI and mII inclusion were also observed in heterozygotes for loss-of-function alleles of virilizer, fl(2)d, and crooked neck. The products of virilizer and fl(2)d are also required for Sxl autoregulation at the level of splicing; crooked neck encodes a protein with structural similarities to yeast-splicing factors Prp39p and Prp42p. Deletion of at least five other loci caused significant reductions in the inclusion of mI and/or mII. Possible roles of identified factors are discussed in the context of the resplicing strategy for generation of alternative Ubx mRNAs. |
doi_str_mv | 10.1093/genetics/151.4.1517 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1460545</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17202307</sourcerecordid><originalsourceid>FETCH-LOGICAL-c579t-4362aaa5e2f532c995ed465c059e0e686c395c3c6efb2dda66aaeb20099f58a13</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhiMEotvCL0CgiAOcsvV31pdKVfmUKpCge7ZmvZPEVRJvbYdt_z1epaCWC7I0c3gfv5qZtyheUbKkRPPTFkdMzsZTKulSLHOtnxQLqgWvmOL0abEghKpK1ZweFccxXhNClJar58URJfnRWiyKdBVgjBXY5Ma2bHL3IZYBbyYXcFs2PpRutP0UnR9L32SlnXpIWcJbP8YslqnDct2nABuXOh_gthx-fDuPB_pD8NHvOtdDOWAPo28hJgwvimcN9BFf3veTYv3p49XFl-ry--evF-eXlZW1TpXgigGARNZIzqzWErdCSUukRoJqpSzX0nKrsNmw7RaUAsANI0TrRq6A8pPibPbdTZsBtxbHPGVvdsENEO6MB2ceK6PrTOt_GSoUkUJmg3f3BsHfTBiTGVy02OdV0E_RKK2UIvX_QVozwjipM_j2H_DaT2HMVzCMCso4lSJDfIZsvl8M2PwdmRJzyN78yd7k1I041IP1m4fbPvgzh52B9zPQubbb53xNHKDvM07Nfr9_ZPV6JhvwBtrgoln_pFrrbKUE478By3TF2g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>214123154</pqid></control><display><type>article</type><title>Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.) ; Hatton, A.R ; Lopez, A.J</creator><creatorcontrib>Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.) ; Hatton, A.R ; Lopez, A.J</creatorcontrib><description>Alternatively spliced Ultrabithorax mRNAs differ by the presence of internal exons mI and mII. Two approaches were used to identify trans-acting factors required for inclusion of these cassette exons. First, mutations in a set of genes implicated in the control of other alternative splicing decisions were tested for dominant effects on the Ubx alternative splicing pattern. To identify additional genes involved in regulation of Ubx splicing, a large collection of deficiencies was tested first for dominant enhancement of the haploinsufficient Ubx haltere phenotype and second for effects on the splicing pattern. Inclusion of the cassette exons in Ubx mRNAs was reduced strongly in heterozygotes for hypomorphic alleles of hrp48, which encodes a member of the hnRNP A/B family and is implicated in control of P-element splicing. Significant reductions of mI and mII inclusion were also observed in heterozygotes for loss-of-function alleles of virilizer, fl(2)d, and crooked neck. The products of virilizer and fl(2)d are also required for Sxl autoregulation at the level of splicing; crooked neck encodes a protein with structural similarities to yeast-splicing factors Prp39p and Prp42p. Deletion of at least five other loci caused significant reductions in the inclusion of mI and/or mII. Possible roles of identified factors are discussed in the context of the resplicing strategy for generation of alternative Ubx mRNAs.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/151.4.1517</identifier><identifier>PMID: 10101174</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>ALTERNATIVE SPLICING ; Animals ; ARN MENSAJERO ; ARN MESSAGER ; Base Sequence ; BINDING PROTEINS ; BODY PARTS ; DNA Primers - genetics ; DNA-Binding Proteins - genetics ; DROSOPHILA MELANOGASTER ; Drosophila melanogaster - genetics ; Drosophila Proteins ; Enhancer Elements, Genetic ; Exons ; Female ; FENOTIPOS ; GEN HOMEOTICO ; GENE HOMEOTIQUE ; Genes ; Genes, Insect ; Genetics ; HALTERES ; HETEROCIGOTOS ; HETEROZYGOTE ; HETEROZYGOTES ; Homeodomain Proteins - genetics ; HOMEOTIC GENES ; Insect Proteins - genetics ; Insects ; Male ; MESSENGER RNA ; MESSENGER RNA BINDING PROTEINS ; MUTACION ; MUTATION ; PARTES DEL CUERPO ; PARTIE DU CORPS ; PHENOTYPE ; PHENOTYPES ; PRECURSORS ; PROTEINAS AGLUTINANTES ; PROTEINE DE LIAISON ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; SPLICING FACTORS ; Transcription Factors ; Transcriptional Activation ; UBX GENE</subject><ispartof>Genetics (Austin), 1999-04, Vol.151 (4), p.1517-1529</ispartof><rights>Copyright Genetics Society of America Apr 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c579t-4362aaa5e2f532c995ed465c059e0e686c395c3c6efb2dda66aaeb20099f58a13</citedby><cites>FETCH-LOGICAL-c579t-4362aaa5e2f532c995ed465c059e0e686c395c3c6efb2dda66aaeb20099f58a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10101174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.)</creatorcontrib><creatorcontrib>Hatton, A.R</creatorcontrib><creatorcontrib>Lopez, A.J</creatorcontrib><title>Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>Alternatively spliced Ultrabithorax mRNAs differ by the presence of internal exons mI and mII. Two approaches were used to identify trans-acting factors required for inclusion of these cassette exons. First, mutations in a set of genes implicated in the control of other alternative splicing decisions were tested for dominant effects on the Ubx alternative splicing pattern. To identify additional genes involved in regulation of Ubx splicing, a large collection of deficiencies was tested first for dominant enhancement of the haploinsufficient Ubx haltere phenotype and second for effects on the splicing pattern. Inclusion of the cassette exons in Ubx mRNAs was reduced strongly in heterozygotes for hypomorphic alleles of hrp48, which encodes a member of the hnRNP A/B family and is implicated in control of P-element splicing. Significant reductions of mI and mII inclusion were also observed in heterozygotes for loss-of-function alleles of virilizer, fl(2)d, and crooked neck. The products of virilizer and fl(2)d are also required for Sxl autoregulation at the level of splicing; crooked neck encodes a protein with structural similarities to yeast-splicing factors Prp39p and Prp42p. Deletion of at least five other loci caused significant reductions in the inclusion of mI and/or mII. Possible roles of identified factors are discussed in the context of the resplicing strategy for generation of alternative Ubx mRNAs.</description><subject>ALTERNATIVE SPLICING</subject><subject>Animals</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Base Sequence</subject><subject>BINDING PROTEINS</subject><subject>BODY PARTS</subject><subject>DNA Primers - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DROSOPHILA MELANOGASTER</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila Proteins</subject><subject>Enhancer Elements, Genetic</subject><subject>Exons</subject><subject>Female</subject><subject>FENOTIPOS</subject><subject>GEN HOMEOTICO</subject><subject>GENE HOMEOTIQUE</subject><subject>Genes</subject><subject>Genes, Insect</subject><subject>Genetics</subject><subject>HALTERES</subject><subject>HETEROCIGOTOS</subject><subject>HETEROZYGOTE</subject><subject>HETEROZYGOTES</subject><subject>Homeodomain Proteins - genetics</subject><subject>HOMEOTIC GENES</subject><subject>Insect Proteins - genetics</subject><subject>Insects</subject><subject>Male</subject><subject>MESSENGER RNA</subject><subject>MESSENGER RNA BINDING PROTEINS</subject><subject>MUTACION</subject><subject>MUTATION</subject><subject>PARTES DEL CUERPO</subject><subject>PARTIE DU CORPS</subject><subject>PHENOTYPE</subject><subject>PHENOTYPES</subject><subject>PRECURSORS</subject><subject>PROTEINAS AGLUTINANTES</subject><subject>PROTEINE DE LIAISON</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>SPLICING FACTORS</subject><subject>Transcription Factors</subject><subject>Transcriptional Activation</subject><subject>UBX GENE</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEotvCL0CgiAOcsvV31pdKVfmUKpCge7ZmvZPEVRJvbYdt_z1epaCWC7I0c3gfv5qZtyheUbKkRPPTFkdMzsZTKulSLHOtnxQLqgWvmOL0abEghKpK1ZweFccxXhNClJar58URJfnRWiyKdBVgjBXY5Ma2bHL3IZYBbyYXcFs2PpRutP0UnR9L32SlnXpIWcJbP8YslqnDct2nABuXOh_gthx-fDuPB_pD8NHvOtdDOWAPo28hJgwvimcN9BFf3veTYv3p49XFl-ry--evF-eXlZW1TpXgigGARNZIzqzWErdCSUukRoJqpSzX0nKrsNmw7RaUAsANI0TrRq6A8pPibPbdTZsBtxbHPGVvdsENEO6MB2ceK6PrTOt_GSoUkUJmg3f3BsHfTBiTGVy02OdV0E_RKK2UIvX_QVozwjipM_j2H_DaT2HMVzCMCso4lSJDfIZsvl8M2PwdmRJzyN78yd7k1I041IP1m4fbPvgzh52B9zPQubbb53xNHKDvM07Nfr9_ZPV6JhvwBtrgoln_pFrrbKUE478By3TF2g</recordid><startdate>199904</startdate><enddate>199904</enddate><creator>Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.)</creator><creator>Hatton, A.R</creator><creator>Lopez, A.J</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199904</creationdate><title>Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster</title><author>Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.) ; Hatton, A.R ; Lopez, A.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-4362aaa5e2f532c995ed465c059e0e686c395c3c6efb2dda66aaeb20099f58a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>ALTERNATIVE SPLICING</topic><topic>Animals</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Base Sequence</topic><topic>BINDING PROTEINS</topic><topic>BODY PARTS</topic><topic>DNA Primers - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DROSOPHILA MELANOGASTER</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila Proteins</topic><topic>Enhancer Elements, Genetic</topic><topic>Exons</topic><topic>Female</topic><topic>FENOTIPOS</topic><topic>GEN HOMEOTICO</topic><topic>GENE HOMEOTIQUE</topic><topic>Genes</topic><topic>Genes, Insect</topic><topic>Genetics</topic><topic>HALTERES</topic><topic>HETEROCIGOTOS</topic><topic>HETEROZYGOTE</topic><topic>HETEROZYGOTES</topic><topic>Homeodomain Proteins - genetics</topic><topic>HOMEOTIC GENES</topic><topic>Insect Proteins - genetics</topic><topic>Insects</topic><topic>Male</topic><topic>MESSENGER RNA</topic><topic>MESSENGER RNA BINDING PROTEINS</topic><topic>MUTACION</topic><topic>MUTATION</topic><topic>PARTES DEL CUERPO</topic><topic>PARTIE DU CORPS</topic><topic>PHENOTYPE</topic><topic>PHENOTYPES</topic><topic>PRECURSORS</topic><topic>PROTEINAS AGLUTINANTES</topic><topic>PROTEINE DE LIAISON</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>SPLICING FACTORS</topic><topic>Transcription Factors</topic><topic>Transcriptional Activation</topic><topic>UBX GENE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.)</creatorcontrib><creatorcontrib>Hatton, A.R</creatorcontrib><creatorcontrib>Lopez, A.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burnette, J.M. (Carnegie Mellon University, Pittsburgh, PA.)</au><au>Hatton, A.R</au><au>Lopez, A.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1999-04</date><risdate>1999</risdate><volume>151</volume><issue>4</issue><spage>1517</spage><epage>1529</epage><pages>1517-1529</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>Alternatively spliced Ultrabithorax mRNAs differ by the presence of internal exons mI and mII. Two approaches were used to identify trans-acting factors required for inclusion of these cassette exons. First, mutations in a set of genes implicated in the control of other alternative splicing decisions were tested for dominant effects on the Ubx alternative splicing pattern. To identify additional genes involved in regulation of Ubx splicing, a large collection of deficiencies was tested first for dominant enhancement of the haploinsufficient Ubx haltere phenotype and second for effects on the splicing pattern. Inclusion of the cassette exons in Ubx mRNAs was reduced strongly in heterozygotes for hypomorphic alleles of hrp48, which encodes a member of the hnRNP A/B family and is implicated in control of P-element splicing. Significant reductions of mI and mII inclusion were also observed in heterozygotes for loss-of-function alleles of virilizer, fl(2)d, and crooked neck. The products of virilizer and fl(2)d are also required for Sxl autoregulation at the level of splicing; crooked neck encodes a protein with structural similarities to yeast-splicing factors Prp39p and Prp42p. Deletion of at least five other loci caused significant reductions in the inclusion of mI and/or mII. Possible roles of identified factors are discussed in the context of the resplicing strategy for generation of alternative Ubx mRNAs.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>10101174</pmid><doi>10.1093/genetics/151.4.1517</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0016-6731 |
ispartof | Genetics (Austin), 1999-04, Vol.151 (4), p.1517-1529 |
issn | 0016-6731 1943-2631 1943-2631 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1460545 |
source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
subjects | ALTERNATIVE SPLICING Animals ARN MENSAJERO ARN MESSAGER Base Sequence BINDING PROTEINS BODY PARTS DNA Primers - genetics DNA-Binding Proteins - genetics DROSOPHILA MELANOGASTER Drosophila melanogaster - genetics Drosophila Proteins Enhancer Elements, Genetic Exons Female FENOTIPOS GEN HOMEOTICO GENE HOMEOTIQUE Genes Genes, Insect Genetics HALTERES HETEROCIGOTOS HETEROZYGOTE HETEROZYGOTES Homeodomain Proteins - genetics HOMEOTIC GENES Insect Proteins - genetics Insects Male MESSENGER RNA MESSENGER RNA BINDING PROTEINS MUTACION MUTATION PARTES DEL CUERPO PARTIE DU CORPS PHENOTYPE PHENOTYPES PRECURSORS PROTEINAS AGLUTINANTES PROTEINE DE LIAISON Ribonucleic acid RNA RNA, Messenger - genetics SPLICING FACTORS Transcription Factors Transcriptional Activation UBX GENE |
title | Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T02%3A31%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Trans-acting%20factors%20required%20for%20inclusion%20of%20regulated%20exons%20in%20the%20Ultrabithorax%20mRNAs%20of%20Drosophila%20melanogaster&rft.jtitle=Genetics%20(Austin)&rft.au=Burnette,%20J.M.%20(Carnegie%20Mellon%20University,%20Pittsburgh,%20PA.)&rft.date=1999-04&rft.volume=151&rft.issue=4&rft.spage=1517&rft.epage=1529&rft.pages=1517-1529&rft.issn=0016-6731&rft.eissn=1943-2631&rft.coden=GENTAE&rft_id=info:doi/10.1093/genetics/151.4.1517&rft_dat=%3Cproquest_pubme%3E17202307%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=214123154&rft_id=info:pmid/10101174&rfr_iscdi=true |