A screen for genes involved in the anaphase proteolytic pathway identifies tsm1+, a novel Schizosaccharomyces pombe gene important for microtubule integrity
The growth of several mitotic mutants of Schizosaccharomyces pombe, including nuc2-663, is inhibited by the protease inhibitor N-Tosyl-L-Phenylalanine Chloromethyl Ketone (TPCK). Because nuc2(+) encodes a presumptive component of the Anaphase Promoting Complex, which is required for the ubiquitin-de...
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| Veröffentlicht in: | Genetics (Austin) 1998-07, Vol.149 (3), p.1251-1264 |
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| creator | Grishchuk, E.L. (University of Colorado, Boulder, CO.) Howe, J.L McIntosh, J.R |
| description | The growth of several mitotic mutants of Schizosaccharomyces pombe, including nuc2-663, is inhibited by the protease inhibitor N-Tosyl-L-Phenylalanine Chloromethyl Ketone (TPCK). Because nuc2(+) encodes a presumptive component of the Anaphase Promoting Complex, which is required for the ubiquitin-dependent proteolysis of certain proteins during exit from mitosis, we have used sensitivity to TPCK as a criterion by which to search for novel S. pombe mutants defective in the anaphase-promoting pathway. In a genetic screen for temperature-sensitive mitotic mutants that were also sensitive to TPCK at a permissive temperature, we isolated three tsm (TPCK-sensitive mitotic) strains. Two of these are alleles of cut1(+), but tsm1-512 maps to a novel genetic location. The tsm1-512 mutation leads to delayed nuclear division at restrictive temperatures, apparently as a result of an impaired ability to form a metaphase spindle. After shift of early G2 cells to 36 degrees, tsm1-512 arrests transiently in the second mitotic division and then exits mitosis, as judged by spindle elongation and septation. The chromosomes, however, often fail to segregate properly. Genetic interactions between tsm1-512 and components of the anaphase proteolytic pathway suggest a functional involvement of the Tsm1 protein in this pathway. |
| doi_str_mv | 10.1093/genetics/149.3.1251 |
| format | Article |
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(University of Colorado, Boulder, CO.) ; Howe, J.L ; McIntosh, J.R</creator><creatorcontrib>Grishchuk, E.L. (University of Colorado, Boulder, CO.) ; Howe, J.L ; McIntosh, J.R</creatorcontrib><description>The growth of several mitotic mutants of Schizosaccharomyces pombe, including nuc2-663, is inhibited by the protease inhibitor N-Tosyl-L-Phenylalanine Chloromethyl Ketone (TPCK). Because nuc2(+) encodes a presumptive component of the Anaphase Promoting Complex, which is required for the ubiquitin-dependent proteolysis of certain proteins during exit from mitosis, we have used sensitivity to TPCK as a criterion by which to search for novel S. pombe mutants defective in the anaphase-promoting pathway. In a genetic screen for temperature-sensitive mitotic mutants that were also sensitive to TPCK at a permissive temperature, we isolated three tsm (TPCK-sensitive mitotic) strains. Two of these are alleles of cut1(+), but tsm1-512 maps to a novel genetic location. The tsm1-512 mutation leads to delayed nuclear division at restrictive temperatures, apparently as a result of an impaired ability to form a metaphase spindle. After shift of early G2 cells to 36 degrees, tsm1-512 arrests transiently in the second mitotic division and then exits mitosis, as judged by spindle elongation and septation. The chromosomes, however, often fail to segregate properly. Genetic interactions between tsm1-512 and components of the anaphase proteolytic pathway suggest a functional involvement of the Tsm1 protein in this pathway.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/149.3.1251</identifier><identifier>PMID: 9649518</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>Anaphase - genetics ; CARTE GENETIQUE ; Cell Cycle - genetics ; CELL DIVISION ; CETONAS ; CETONE ; CHROMOSOME SEGREGATION ; CYTOPLASMIC ORGANELLES ; DIVISION CELLULAIRE ; DIVISION CELULAR ; DNA-Binding Proteins - biosynthesis ; DNA-Binding Proteins - genetics ; FENOTIPOS ; Fungal Proteins - genetics ; GENE ; GENE LOCATION ; GENES ; GENETIC MAPPING ; GENETIC MAPS ; Genetic Testing ; Genetics ; Genotype ; INHIBIDORES DE PROTEINASAS ; INHIBITEUR DE PROTEINASES ; KETONES ; LOCALISATION DE GENE ; LOCALIZACION DE GENES ; MAPAS GENETICOS ; METAPHASE SPINDLE ; MICROTUBULE ; MICROTUBULES ; Microtubules - genetics ; Microtubules - ultrastructure ; MICROTUBULOS ; MITOSE ; MITOSIS ; Mitosis - genetics ; MITOTIC SPINDLE ; Models, Genetic ; Molecular biology ; Mutagenesis ; MUTANT ; MUTANTES ; MUTANTS ; Mutation ; N-TOSYL-L-PHENYLALANINE CHLOROMETHYL KETONE ; ORGANITE CELLULAIRE ; ORGANULOS CITOPLASMICOS ; PHENOTYPE ; PHENOTYPES ; PROTEINAS ; PROTEINASE INHIBITORS ; PROTEINE ; PROTEINS ; Saccharomyces cerevisiae Proteins ; Schizosaccharomyces - genetics ; Schizosaccharomyces - ultrastructure ; SCHIZOSACCHAROMYCES POMBE ; SUSCEPTIBILITY ; TATA-Binding Protein Associated Factors ; Tosylphenylalanyl Chloromethyl Ketone ; Transcription Factor TFIID ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; TSM1+ GENE</subject><ispartof>Genetics (Austin), 1998-07, Vol.149 (3), p.1251-1264</ispartof><rights>Copyright Genetics Society of America Jul 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-26aa2d498dca65eecc9a4c56e6ead87907659b83979c0865076f5ad0918d86ea3</citedby><cites>FETCH-LOGICAL-c481t-26aa2d498dca65eecc9a4c56e6ead87907659b83979c0865076f5ad0918d86ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9649518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grishchuk, E.L. (University of Colorado, Boulder, CO.)</creatorcontrib><creatorcontrib>Howe, J.L</creatorcontrib><creatorcontrib>McIntosh, J.R</creatorcontrib><title>A screen for genes involved in the anaphase proteolytic pathway identifies tsm1+, a novel Schizosaccharomyces pombe gene important for microtubule integrity</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>The growth of several mitotic mutants of Schizosaccharomyces pombe, including nuc2-663, is inhibited by the protease inhibitor N-Tosyl-L-Phenylalanine Chloromethyl Ketone (TPCK). Because nuc2(+) encodes a presumptive component of the Anaphase Promoting Complex, which is required for the ubiquitin-dependent proteolysis of certain proteins during exit from mitosis, we have used sensitivity to TPCK as a criterion by which to search for novel S. pombe mutants defective in the anaphase-promoting pathway. In a genetic screen for temperature-sensitive mitotic mutants that were also sensitive to TPCK at a permissive temperature, we isolated three tsm (TPCK-sensitive mitotic) strains. Two of these are alleles of cut1(+), but tsm1-512 maps to a novel genetic location. The tsm1-512 mutation leads to delayed nuclear division at restrictive temperatures, apparently as a result of an impaired ability to form a metaphase spindle. After shift of early G2 cells to 36 degrees, tsm1-512 arrests transiently in the second mitotic division and then exits mitosis, as judged by spindle elongation and septation. The chromosomes, however, often fail to segregate properly. Genetic interactions between tsm1-512 and components of the anaphase proteolytic pathway suggest a functional involvement of the Tsm1 protein in this pathway.</description><subject>Anaphase - genetics</subject><subject>CARTE GENETIQUE</subject><subject>Cell Cycle - genetics</subject><subject>CELL DIVISION</subject><subject>CETONAS</subject><subject>CETONE</subject><subject>CHROMOSOME SEGREGATION</subject><subject>CYTOPLASMIC ORGANELLES</subject><subject>DIVISION CELLULAIRE</subject><subject>DIVISION CELULAR</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>FENOTIPOS</subject><subject>Fungal Proteins - genetics</subject><subject>GENE</subject><subject>GENE LOCATION</subject><subject>GENES</subject><subject>GENETIC MAPPING</subject><subject>GENETIC MAPS</subject><subject>Genetic Testing</subject><subject>Genetics</subject><subject>Genotype</subject><subject>INHIBIDORES DE PROTEINASAS</subject><subject>INHIBITEUR DE PROTEINASES</subject><subject>KETONES</subject><subject>LOCALISATION DE GENE</subject><subject>LOCALIZACION DE GENES</subject><subject>MAPAS GENETICOS</subject><subject>METAPHASE SPINDLE</subject><subject>MICROTUBULE</subject><subject>MICROTUBULES</subject><subject>Microtubules - genetics</subject><subject>Microtubules - ultrastructure</subject><subject>MICROTUBULOS</subject><subject>MITOSE</subject><subject>MITOSIS</subject><subject>Mitosis - genetics</subject><subject>MITOTIC SPINDLE</subject><subject>Models, Genetic</subject><subject>Molecular biology</subject><subject>Mutagenesis</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Mutation</subject><subject>N-TOSYL-L-PHENYLALANINE CHLOROMETHYL KETONE</subject><subject>ORGANITE CELLULAIRE</subject><subject>ORGANULOS CITOPLASMICOS</subject><subject>PHENOTYPE</subject><subject>PHENOTYPES</subject><subject>PROTEINAS</subject><subject>PROTEINASE INHIBITORS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - ultrastructure</subject><subject>SCHIZOSACCHAROMYCES POMBE</subject><subject>SUSCEPTIBILITY</subject><subject>TATA-Binding Protein Associated Factors</subject><subject>Tosylphenylalanyl Chloromethyl Ketone</subject><subject>Transcription Factor TFIID</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - genetics</subject><subject>TSM1+ GENE</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptUU1v1DAUjBCoLIVfgJAsLhxgt3YcO_GlUlXxJVXiUHq23jovG1dJHGxno_Bb-LF4u0sFEifbmnkzbzxZ9prRDaOKX-xwwGhNuGCF2vANywV7kq2YKvg6l5w9zVaUMrmWJWfPsxch3FNKpRLVWXamZKEEq1bZrysSjEccSOM8OSgGYoe96_ZYpwuJLRIYYGwhIBm9i-i6JZmSEWI7w0JsjUO0jU1zMfTs_QcCZHB77Mitae1PF8CYFrzrF5Moo-u3-GBDbD86H2GID869NUl82k5dQoaIO2_j8jJ71kAX8NXpPM_uPn38fv1lffPt89frq5u1KSoWU1iAvC5UVRuQAtEYBYUREiVCXZWKllKobcVVqQytpEjvRkBNFavqKnH4eXZ51B2nbY-1SYk8dHr0tge_aAdW_4sMttU7t9eskDTnNAm8PQl492PCEPW9m_yQdtY5K1iukmki8SMpJQ3BY_NowKg-FKr_FJp0leb6UGiaevP3bo8zpwYT_u6It3bXztajDj10XWIzPc_z_5QacBrSBwd9d8uUKmkphJD8N1oOugs</recordid><startdate>199807</startdate><enddate>199807</enddate><creator>Grishchuk, E.L. (University of Colorado, Boulder, CO.)</creator><creator>Howe, J.L</creator><creator>McIntosh, J.R</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>199807</creationdate><title>A screen for genes involved in the anaphase proteolytic pathway identifies tsm1+, a novel Schizosaccharomyces pombe gene important for microtubule integrity</title><author>Grishchuk, E.L. (University of Colorado, Boulder, CO.) ; Howe, J.L ; McIntosh, J.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-26aa2d498dca65eecc9a4c56e6ead87907659b83979c0865076f5ad0918d86ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Anaphase - genetics</topic><topic>CARTE GENETIQUE</topic><topic>Cell Cycle - genetics</topic><topic>CELL DIVISION</topic><topic>CETONAS</topic><topic>CETONE</topic><topic>CHROMOSOME SEGREGATION</topic><topic>CYTOPLASMIC ORGANELLES</topic><topic>DIVISION CELLULAIRE</topic><topic>DIVISION CELULAR</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>FENOTIPOS</topic><topic>Fungal Proteins - genetics</topic><topic>GENE</topic><topic>GENE LOCATION</topic><topic>GENES</topic><topic>GENETIC MAPPING</topic><topic>GENETIC MAPS</topic><topic>Genetic Testing</topic><topic>Genetics</topic><topic>Genotype</topic><topic>INHIBIDORES DE PROTEINASAS</topic><topic>INHIBITEUR DE PROTEINASES</topic><topic>KETONES</topic><topic>LOCALISATION DE GENE</topic><topic>LOCALIZACION DE GENES</topic><topic>MAPAS GENETICOS</topic><topic>METAPHASE SPINDLE</topic><topic>MICROTUBULE</topic><topic>MICROTUBULES</topic><topic>Microtubules - genetics</topic><topic>Microtubules - ultrastructure</topic><topic>MICROTUBULOS</topic><topic>MITOSE</topic><topic>MITOSIS</topic><topic>Mitosis - genetics</topic><topic>MITOTIC SPINDLE</topic><topic>Models, Genetic</topic><topic>Molecular biology</topic><topic>Mutagenesis</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Mutation</topic><topic>N-TOSYL-L-PHENYLALANINE CHLOROMETHYL KETONE</topic><topic>ORGANITE CELLULAIRE</topic><topic>ORGANULOS CITOPLASMICOS</topic><topic>PHENOTYPE</topic><topic>PHENOTYPES</topic><topic>PROTEINAS</topic><topic>PROTEINASE INHIBITORS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - ultrastructure</topic><topic>SCHIZOSACCHAROMYCES POMBE</topic><topic>SUSCEPTIBILITY</topic><topic>TATA-Binding Protein Associated Factors</topic><topic>Tosylphenylalanyl Chloromethyl Ketone</topic><topic>Transcription Factor TFIID</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - genetics</topic><topic>TSM1+ GENE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grishchuk, E.L. (University of Colorado, Boulder, CO.)</creatorcontrib><creatorcontrib>Howe, J.L</creatorcontrib><creatorcontrib>McIntosh, J.R</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grishchuk, E.L. (University of Colorado, Boulder, CO.)</au><au>Howe, J.L</au><au>McIntosh, J.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A screen for genes involved in the anaphase proteolytic pathway identifies tsm1+, a novel Schizosaccharomyces pombe gene important for microtubule integrity</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1998-07</date><risdate>1998</risdate><volume>149</volume><issue>3</issue><spage>1251</spage><epage>1264</epage><pages>1251-1264</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>The growth of several mitotic mutants of Schizosaccharomyces pombe, including nuc2-663, is inhibited by the protease inhibitor N-Tosyl-L-Phenylalanine Chloromethyl Ketone (TPCK). Because nuc2(+) encodes a presumptive component of the Anaphase Promoting Complex, which is required for the ubiquitin-dependent proteolysis of certain proteins during exit from mitosis, we have used sensitivity to TPCK as a criterion by which to search for novel S. pombe mutants defective in the anaphase-promoting pathway. In a genetic screen for temperature-sensitive mitotic mutants that were also sensitive to TPCK at a permissive temperature, we isolated three tsm (TPCK-sensitive mitotic) strains. Two of these are alleles of cut1(+), but tsm1-512 maps to a novel genetic location. The tsm1-512 mutation leads to delayed nuclear division at restrictive temperatures, apparently as a result of an impaired ability to form a metaphase spindle. After shift of early G2 cells to 36 degrees, tsm1-512 arrests transiently in the second mitotic division and then exits mitosis, as judged by spindle elongation and septation. The chromosomes, however, often fail to segregate properly. Genetic interactions between tsm1-512 and components of the anaphase proteolytic pathway suggest a functional involvement of the Tsm1 protein in this pathway.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>9649518</pmid><doi>10.1093/genetics/149.3.1251</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0016-6731 |
| ispartof | Genetics (Austin), 1998-07, Vol.149 (3), p.1251-1264 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1460230 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Anaphase - genetics CARTE GENETIQUE Cell Cycle - genetics CELL DIVISION CETONAS CETONE CHROMOSOME SEGREGATION CYTOPLASMIC ORGANELLES DIVISION CELLULAIRE DIVISION CELULAR DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics FENOTIPOS Fungal Proteins - genetics GENE GENE LOCATION GENES GENETIC MAPPING GENETIC MAPS Genetic Testing Genetics Genotype INHIBIDORES DE PROTEINASAS INHIBITEUR DE PROTEINASES KETONES LOCALISATION DE GENE LOCALIZACION DE GENES MAPAS GENETICOS METAPHASE SPINDLE MICROTUBULE MICROTUBULES Microtubules - genetics Microtubules - ultrastructure MICROTUBULOS MITOSE MITOSIS Mitosis - genetics MITOTIC SPINDLE Models, Genetic Molecular biology Mutagenesis MUTANT MUTANTES MUTANTS Mutation N-TOSYL-L-PHENYLALANINE CHLOROMETHYL KETONE ORGANITE CELLULAIRE ORGANULOS CITOPLASMICOS PHENOTYPE PHENOTYPES PROTEINAS PROTEINASE INHIBITORS PROTEINE PROTEINS Saccharomyces cerevisiae Proteins Schizosaccharomyces - genetics Schizosaccharomyces - ultrastructure SCHIZOSACCHAROMYCES POMBE SUSCEPTIBILITY TATA-Binding Protein Associated Factors Tosylphenylalanyl Chloromethyl Ketone Transcription Factor TFIID Transcription Factors - biosynthesis Transcription Factors - genetics TSM1+ GENE |
| title | A screen for genes involved in the anaphase proteolytic pathway identifies tsm1+, a novel Schizosaccharomyces pombe gene important for microtubule integrity |
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