DNA Replication Initiates Non-Randomly at Multiple Sites Near the c-myc Gene in HeLa Cells
The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5′ to the P1 promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template...
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| Veröffentlicht in: | Nucleic acids research 1996-05, Vol.24 (10), p.1887-1894 |
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| container_end_page | 1894 |
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| container_issue | 10 |
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| container_title | Nucleic acids research |
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| creator | Waltz, Susan E. Trivedi, Alpa A. Leffak, Michael |
| description | The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5′ to the P1 promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DNAs in a 12 kb c-myc domain has been analyzed by hybridization to strand specific probes. Strong switches in the asymmetry of nascent strand template preference confirm that replication initiates non-randomly at multiple sites within 2.4 kb 5′ to the c-myc P1 promoter, and at other sites over a region of 12 kb or more. The strongest template biases occur in the 2.4 kb region 5′ of the P1 promoter, shown earlier to contain sequences which allow the autonomous semiconservative replication of c-myc plasmids. An asymmetric pyrimidine heptanucleotide consensus sequence has been identified which occurs 12 times in the c-myc origin zone, and whose polarity exactly correlates with the polarity of nascent strand synthesis. |
| doi_str_mv | 10.1093/nar/24.10.1887 |
| format | Article |
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To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DNAs in a 12 kb c-myc domain has been analyzed by hybridization to strand specific probes. Strong switches in the asymmetry of nascent strand template preference confirm that replication initiates non-randomly at multiple sites within 2.4 kb 5′ to the c-myc P1 promoter, and at other sites over a region of 12 kb or more. The strongest template biases occur in the 2.4 kb region 5′ of the P1 promoter, shown earlier to contain sequences which allow the autonomous semiconservative replication of c-myc plasmids. An asymmetric pyrimidine heptanucleotide consensus sequence has been identified which occurs 12 times in the c-myc origin zone, and whose polarity exactly correlates with the polarity of nascent strand synthesis.</description><identifier>ISSN: 0305-1048</identifier><identifier>ISSN: 1362-4962</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/24.10.1887</identifier><identifier>PMID: 8657570</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Base Sequence ; Consensus Sequence ; DNA - analysis ; DNA - chemistry ; DNA Probes ; DNA Replication ; Electrophoresis, Agar Gel ; Genes, myc - genetics ; HeLa Cells - metabolism ; Humans ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Promoter Regions, Genetic ; RNA</subject><ispartof>Nucleic acids research, 1996-05, Vol.24 (10), p.1887-1894</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-3754d695cb37d27afe87266a897d8fb7758b22b260252c7813e207f8c45046c63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC145880/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC145880/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8657570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Waltz, Susan E.</creatorcontrib><creatorcontrib>Trivedi, Alpa A.</creatorcontrib><creatorcontrib>Leffak, Michael</creatorcontrib><title>DNA Replication Initiates Non-Randomly at Multiple Sites Near the c-myc Gene in HeLa Cells</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Research</addtitle><description>The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5′ to the P1 promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DNAs in a 12 kb c-myc domain has been analyzed by hybridization to strand specific probes. Strong switches in the asymmetry of nascent strand template preference confirm that replication initiates non-randomly at multiple sites within 2.4 kb 5′ to the c-myc P1 promoter, and at other sites over a region of 12 kb or more. The strongest template biases occur in the 2.4 kb region 5′ of the P1 promoter, shown earlier to contain sequences which allow the autonomous semiconservative replication of c-myc plasmids. An asymmetric pyrimidine heptanucleotide consensus sequence has been identified which occurs 12 times in the c-myc origin zone, and whose polarity exactly correlates with the polarity of nascent strand synthesis.</description><subject>Base Sequence</subject><subject>Consensus Sequence</subject><subject>DNA - analysis</subject><subject>DNA - chemistry</subject><subject>DNA Probes</subject><subject>DNA Replication</subject><subject>Electrophoresis, Agar Gel</subject><subject>Genes, myc - genetics</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Promoter Regions, Genetic</subject><subject>RNA</subject><issn>0305-1048</issn><issn>1362-4962</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEUxC0EKmngyg3JJ26b-tvOgUMJbVMRWlEKinqxvF4vNex6g-1UzX-P20QRPfVkPf1mrDdvAHiH0QSjKT0KJh4RNnkYlZIvwAhTQSo2FeQlGCGKeIURU6_BYUq_EcIMc3YADpTgkks0AjefL47hlVt13prshwDPg8_eZJfgxRCqKxOaoe820GT4dd1lv-oc_O4fsTMR5lsHbdVvLDxzwUEf4NwtDJy5rktvwKvWdMm93b1j8OP05Ho2rxaXZ-ez40VlOUe5opKzRky5ralsiDStU5IIYdRUNqqtpeSqJqQmAhFOrFSYOoJkqyzjiAkr6Bh83P67Wte9a6wLOZpOr6LvTdzowXj9lAR_q38NdxozrhQq_g87fxz-rl3KuvfJlgQmuGGdtFSIMYr5s0Jcjl7SiOeFvNyelSRjMNkKbRxSiq7db42RfqhXl3o1YY9jqbcY3v-fdS_f9Vl4teU-ZXe_xyb-0UKW3fR8eaOvf55--_SFLfWS_gMupa7Y</recordid><startdate>19960515</startdate><enddate>19960515</enddate><creator>Waltz, Susan E.</creator><creator>Trivedi, Alpa A.</creator><creator>Leffak, Michael</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960515</creationdate><title>DNA Replication Initiates Non-Randomly at Multiple Sites Near the c-myc Gene in HeLa Cells</title><author>Waltz, Susan E. ; Trivedi, Alpa A. ; Leffak, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-3754d695cb37d27afe87266a897d8fb7758b22b260252c7813e207f8c45046c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Base Sequence</topic><topic>Consensus Sequence</topic><topic>DNA - analysis</topic><topic>DNA - chemistry</topic><topic>DNA Probes</topic><topic>DNA Replication</topic><topic>Electrophoresis, Agar Gel</topic><topic>Genes, myc - genetics</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Promoter Regions, Genetic</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Waltz, Susan E.</creatorcontrib><creatorcontrib>Trivedi, Alpa A.</creatorcontrib><creatorcontrib>Leffak, Michael</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Waltz, Susan E.</au><au>Trivedi, Alpa A.</au><au>Leffak, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Replication Initiates Non-Randomly at Multiple Sites Near the c-myc Gene in HeLa Cells</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Research</addtitle><date>1996-05-15</date><risdate>1996</risdate><volume>24</volume><issue>10</issue><spage>1887</spage><epage>1894</epage><pages>1887-1894</pages><issn>0305-1048</issn><issn>1362-4962</issn><eissn>1362-4962</eissn><abstract>The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5′ to the P1 promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DNAs in a 12 kb c-myc domain has been analyzed by hybridization to strand specific probes. Strong switches in the asymmetry of nascent strand template preference confirm that replication initiates non-randomly at multiple sites within 2.4 kb 5′ to the c-myc P1 promoter, and at other sites over a region of 12 kb or more. The strongest template biases occur in the 2.4 kb region 5′ of the P1 promoter, shown earlier to contain sequences which allow the autonomous semiconservative replication of c-myc plasmids. An asymmetric pyrimidine heptanucleotide consensus sequence has been identified which occurs 12 times in the c-myc origin zone, and whose polarity exactly correlates with the polarity of nascent strand synthesis.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>8657570</pmid><doi>10.1093/nar/24.10.1887</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1996-05, Vol.24 (10), p.1887-1894 |
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| source | MEDLINE; Oxford Journals Open Access Collection; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Base Sequence Consensus Sequence DNA - analysis DNA - chemistry DNA Probes DNA Replication Electrophoresis, Agar Gel Genes, myc - genetics HeLa Cells - metabolism Humans Molecular Sequence Data Nucleic Acid Hybridization Promoter Regions, Genetic RNA |
| title | DNA Replication Initiates Non-Randomly at Multiple Sites Near the c-myc Gene in HeLa Cells |
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