The Small Subunit of the Splicing Factor U2AF Is Conserved in Fission Yeast
The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified the Schizosaccharomyces pombe homolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact wi...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1996-05, Vol.24 (10), p.1849-1854 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1854 |
|---|---|
| container_issue | 10 |
| container_start_page | 1849 |
| container_title | Nucleic acids research |
| container_volume | 24 |
| creator | Wentz-Hunter, Kelly Potashkin, Judith |
| description | The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified the Schizosaccharomyces pombe homolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF59 using the yeast two-hybrid system and have identified a homolog of the hsU2AF35 subunit. The S.pombe U2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit. Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa. The region of spU2AF59 that interacts with spU2AF23 is similar to the region in which the human small and large subunits interact. |
| doi_str_mv | 10.1093/nar/24.10.1849 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_145878</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78046028</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-38bcc19a8df3cfd4d4bba0d509756144dfe4559658a9d73d2c934f10cacdf9623</originalsourceid><addsrcrecordid>eNqFkc1vEzEQxS0EKqFw5YbkE7dN_THetQ8cqoiQikggtUXAxfLa3tawsVN7t6L_fTckiuDU02jm_d5oRg-ht5TMKVH8LJp8xmC-ayWoZ2hGec0qUDV7jmaEE1FRAvIlelXKL0IoUAEn6ETWohG1mKHPV7ceX25M3-PLsR1jGHDq8LAbbvtgQ7zBS2OHlPE1O1_ii4IXKRaf773DIeJlKCWkiH94U4bX6EVn-uLfHOopul5-vFqsqvWXTxeL83VlQcJQcdlaS5WRruO2c-CgbQ1xgqjpJArgOg9CqFpIo1zDHbOKQ0eJNdZ101_8FH3Y792O7cY76-OQTa-3OWxMftDJBP2_EsOtvkn3moKQjZz87w_-nO5GXwa9CcX6vjfRp7HoRhKoCXsapKqmiin-NChEwwTbgfM9aHMqJfvueDUlepennvLUDP62U56T4d2_vx7xQ4CTXu31UAb_5yib_FvXDW-EXn3_qdlq0Xz9Jtda8kcfdqrc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15572523</pqid></control><display><type>article</type><title>The Small Subunit of the Splicing Factor U2AF Is Conserved in Fission Yeast</title><source>MEDLINE</source><source>Access via Oxford University Press (Open Access Collection)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Wentz-Hunter, Kelly ; Potashkin, Judith</creator><creatorcontrib>Wentz-Hunter, Kelly ; Potashkin, Judith</creatorcontrib><description>The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified the Schizosaccharomyces pombe homolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF59 using the yeast two-hybrid system and have identified a homolog of the hsU2AF35 subunit. The S.pombe U2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit. Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa. The region of spU2AF59 that interacts with spU2AF23 is similar to the region in which the human small and large subunits interact.</description><identifier>ISSN: 0305-1048</identifier><identifier>ISSN: 1362-4962</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/24.10.1849</identifier><identifier>PMID: 8657565</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Amino Acid Sequence ; Base Sequence ; beta-Galactosidase - genetics ; Binding Sites ; Blotting, Southern ; Cloning, Molecular ; DNA, Complementary ; DNA-Binding Proteins ; Fungal Proteins - genetics ; Humans ; Molecular Sequence Data ; Nuclear Proteins ; Promoter Regions, Genetic ; Recombinant Fusion Proteins ; Ribonucleoproteins - chemistry ; Ribonucleoproteins - genetics ; Ribonucleoproteins - metabolism ; RNA Splicing ; Saccharomyces cerevisiae Proteins ; Schizosaccharomyces - chemistry ; Schizosaccharomyces - genetics ; Schizosaccharomyces pombe ; Sequence Analysis ; Sequence Homology ; Splicing Factor U2AF ; Transcription Factors</subject><ispartof>Nucleic acids research, 1996-05, Vol.24 (10), p.1849-1854</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-38bcc19a8df3cfd4d4bba0d509756144dfe4559658a9d73d2c934f10cacdf9623</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC145878/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC145878/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8657565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wentz-Hunter, Kelly</creatorcontrib><creatorcontrib>Potashkin, Judith</creatorcontrib><title>The Small Subunit of the Splicing Factor U2AF Is Conserved in Fission Yeast</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Research</addtitle><description>The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified the Schizosaccharomyces pombe homolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF59 using the yeast two-hybrid system and have identified a homolog of the hsU2AF35 subunit. The S.pombe U2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit. Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa. The region of spU2AF59 that interacts with spU2AF23 is similar to the region in which the human small and large subunits interact.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - genetics</subject><subject>Binding Sites</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>DNA-Binding Proteins</subject><subject>Fungal Proteins - genetics</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins</subject><subject>Ribonucleoproteins - chemistry</subject><subject>Ribonucleoproteins - genetics</subject><subject>Ribonucleoproteins - metabolism</subject><subject>RNA Splicing</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Schizosaccharomyces - chemistry</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces pombe</subject><subject>Sequence Analysis</subject><subject>Sequence Homology</subject><subject>Splicing Factor U2AF</subject><subject>Transcription Factors</subject><issn>0305-1048</issn><issn>1362-4962</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EKqFw5YbkE7dN_THetQ8cqoiQikggtUXAxfLa3tawsVN7t6L_fTckiuDU02jm_d5oRg-ht5TMKVH8LJp8xmC-ayWoZ2hGec0qUDV7jmaEE1FRAvIlelXKL0IoUAEn6ETWohG1mKHPV7ceX25M3-PLsR1jGHDq8LAbbvtgQ7zBS2OHlPE1O1_ii4IXKRaf773DIeJlKCWkiH94U4bX6EVn-uLfHOopul5-vFqsqvWXTxeL83VlQcJQcdlaS5WRruO2c-CgbQ1xgqjpJArgOg9CqFpIo1zDHbOKQ0eJNdZ101_8FH3Y792O7cY76-OQTa-3OWxMftDJBP2_EsOtvkn3moKQjZz87w_-nO5GXwa9CcX6vjfRp7HoRhKoCXsapKqmiin-NChEwwTbgfM9aHMqJfvueDUlepennvLUDP62U56T4d2_vx7xQ4CTXu31UAb_5yib_FvXDW-EXn3_qdlq0Xz9Jtda8kcfdqrc</recordid><startdate>19960515</startdate><enddate>19960515</enddate><creator>Wentz-Hunter, Kelly</creator><creator>Potashkin, Judith</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960515</creationdate><title>The Small Subunit of the Splicing Factor U2AF Is Conserved in Fission Yeast</title><author>Wentz-Hunter, Kelly ; Potashkin, Judith</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-38bcc19a8df3cfd4d4bba0d509756144dfe4559658a9d73d2c934f10cacdf9623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>beta-Galactosidase - genetics</topic><topic>Binding Sites</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>DNA-Binding Proteins</topic><topic>Fungal Proteins - genetics</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins</topic><topic>Ribonucleoproteins - chemistry</topic><topic>Ribonucleoproteins - genetics</topic><topic>Ribonucleoproteins - metabolism</topic><topic>RNA Splicing</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Schizosaccharomyces - chemistry</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces pombe</topic><topic>Sequence Analysis</topic><topic>Sequence Homology</topic><topic>Splicing Factor U2AF</topic><topic>Transcription Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wentz-Hunter, Kelly</creatorcontrib><creatorcontrib>Potashkin, Judith</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wentz-Hunter, Kelly</au><au>Potashkin, Judith</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Small Subunit of the Splicing Factor U2AF Is Conserved in Fission Yeast</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Research</addtitle><date>1996-05-15</date><risdate>1996</risdate><volume>24</volume><issue>10</issue><spage>1849</spage><epage>1854</epage><pages>1849-1854</pages><issn>0305-1048</issn><issn>1362-4962</issn><eissn>1362-4962</eissn><abstract>The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified the Schizosaccharomyces pombe homolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF59 using the yeast two-hybrid system and have identified a homolog of the hsU2AF35 subunit. The S.pombe U2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit. Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa. The region of spU2AF59 that interacts with spU2AF23 is similar to the region in which the human small and large subunits interact.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>8657565</pmid><doi>10.1093/nar/24.10.1849</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1996-05, Vol.24 (10), p.1849-1854 |
| issn | 0305-1048 1362-4962 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_145878 |
| source | MEDLINE; Access via Oxford University Press (Open Access Collection); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Base Sequence beta-Galactosidase - genetics Binding Sites Blotting, Southern Cloning, Molecular DNA, Complementary DNA-Binding Proteins Fungal Proteins - genetics Humans Molecular Sequence Data Nuclear Proteins Promoter Regions, Genetic Recombinant Fusion Proteins Ribonucleoproteins - chemistry Ribonucleoproteins - genetics Ribonucleoproteins - metabolism RNA Splicing Saccharomyces cerevisiae Proteins Schizosaccharomyces - chemistry Schizosaccharomyces - genetics Schizosaccharomyces pombe Sequence Analysis Sequence Homology Splicing Factor U2AF Transcription Factors |
| title | The Small Subunit of the Splicing Factor U2AF Is Conserved in Fission Yeast |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T06%3A25%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Small%20Subunit%20of%20the%20Splicing%20Factor%20U2AF%20Is%20Conserved%20in%20Fission%20Yeast&rft.jtitle=Nucleic%20acids%20research&rft.au=Wentz-Hunter,%20Kelly&rft.date=1996-05-15&rft.volume=24&rft.issue=10&rft.spage=1849&rft.epage=1854&rft.pages=1849-1854&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/24.10.1849&rft_dat=%3Cproquest_pubme%3E78046028%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15572523&rft_id=info:pmid/8657565&rfr_iscdi=true |