Defined Culture Conditions of Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2006-04, Vol.103 (15), p.5688-5693 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5693 |
|---|---|
| container_issue | 15 |
| container_start_page | 5688 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 103 |
| creator | Lu, Jean Hou, Runhua Booth, Carmen Jane Yang, Shih-Hung Snyder, Michael |
| description | Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by the difficulties of eliminating animal products in the culture medium and/or the complexity of conditions required to support hESC growth. We have developed a simple medium [termed hESC Cocktail (HESCO)] containing basic fibroblast growth factor, Wnt3a, April (a proliferation-inducing ligand)/BAFF (B cell-activating factor belonging to TNF), albumin, cholesterol, insulin, and transferrin, which is sufficient for hESC self-renewal and proliferation. Cells grown in HESCO were maintained in an undifferentiated state as determined by using six different stem cell markers, and their genomic integrity was confirmed by karyotyping. Cells cultured in HESCO readily form embryoid bodies in tissue culture and teratomas in mice. In both cases, the cells differentiated into each of the three cell lineages, ectoderm, endoderm, and mesoderm, indicating that they maintained their pluripotency. The use of a minimal medium sufficient for hESC growth is expected to greatly facilitate clinical application and developmental studies of hESCs. |
| doi_str_mv | 10.1073/pnas.0601383103 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1458634</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30050161</jstor_id><sourcerecordid>30050161</sourcerecordid><originalsourceid>FETCH-LOGICAL-c594t-86249b98815a53de252b4e511d51b3535ba424c1a6973f66cae3050e0b1cec303</originalsourceid><addsrcrecordid>eNpdkc1v1DAQxS0EotuWc0-giENvaWcytje-IKHQD6RKHChny3GcklViL3aC1P8er3bVD05zmN-8mTePsTOEC4Q1XW69SRcgAakmBHrDVggKS8kVvGUrgGpd1rziR-w4pQ0AKFHDe3aEUighK75i9TfXD951RbOM8xJd0QTfDfMQfCpCX9wuk_HF1dTGx-AHW_yc3VQ0bhzTKXvXmzG5D4d6wn5dX903t-Xdj5vvzde70grF57LOW1Sr6hqFEdS5SlQtdwKxE9iSINGafJ9FI9WaeimtcQQCHLRonSWgE_Zlr7td2sl11vk5mlFv4zCZ-KiDGfTrjh9-64fwVyMXtSSeBc4PAjH8WVya9TQkmy0Y78KSNCpOREJm8PN_4CYs0WdzusoPlgrETu1yD9kYUoquf7oEQe8i0btI9HMkeeLTSwPP_CGDDHzcA5s0h_jUJ8h_QIn0D1-RkAM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201369054</pqid></control><display><type>article</type><title>Defined Culture Conditions of Human Embryonic Stem Cells</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Lu, Jean ; Hou, Runhua ; Booth, Carmen Jane ; Yang, Shih-Hung ; Snyder, Michael</creator><creatorcontrib>Lu, Jean ; Hou, Runhua ; Booth, Carmen Jane ; Yang, Shih-Hung ; Snyder, Michael</creatorcontrib><description>Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by the difficulties of eliminating animal products in the culture medium and/or the complexity of conditions required to support hESC growth. We have developed a simple medium [termed hESC Cocktail (HESCO)] containing basic fibroblast growth factor, Wnt3a, April (a proliferation-inducing ligand)/BAFF (B cell-activating factor belonging to TNF), albumin, cholesterol, insulin, and transferrin, which is sufficient for hESC self-renewal and proliferation. Cells grown in HESCO were maintained in an undifferentiated state as determined by using six different stem cell markers, and their genomic integrity was confirmed by karyotyping. Cells cultured in HESCO readily form embryoid bodies in tissue culture and teratomas in mice. In both cases, the cells differentiated into each of the three cell lineages, ectoderm, endoderm, and mesoderm, indicating that they maintained their pluripotency. The use of a minimal medium sufficient for hESC growth is expected to greatly facilitate clinical application and developmental studies of hESCs.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0601383103</identifier><identifier>PMID: 16595624</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>B lymphocytes ; Biological Sciences ; Biomarkers - analysis ; Biotechnology ; Cell Culture Techniques - methods ; Cell Division - drug effects ; Cell lines ; Cellular biology ; Cellular differentiation ; Culture Media ; Cultured cells ; Cytokines - pharmacology ; Embryo, Mammalian ; Embryonic stem cells ; Embryos ; Feeder cells ; Human growth ; Humans ; Karyotyping ; Neural stem cells ; Pluripotent stem cells ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - drug effects ; Stem cells</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2006-04, Vol.103 (15), p.5688-5693</ispartof><rights>Copyright 2006 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Apr 11, 2006</rights><rights>2006 by The National Academy of Sciences of the USA 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-86249b98815a53de252b4e511d51b3535ba424c1a6973f66cae3050e0b1cec303</citedby><cites>FETCH-LOGICAL-c594t-86249b98815a53de252b4e511d51b3535ba424c1a6973f66cae3050e0b1cec303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30050161$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30050161$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16595624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Jean</creatorcontrib><creatorcontrib>Hou, Runhua</creatorcontrib><creatorcontrib>Booth, Carmen Jane</creatorcontrib><creatorcontrib>Yang, Shih-Hung</creatorcontrib><creatorcontrib>Snyder, Michael</creatorcontrib><title>Defined Culture Conditions of Human Embryonic Stem Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by the difficulties of eliminating animal products in the culture medium and/or the complexity of conditions required to support hESC growth. We have developed a simple medium [termed hESC Cocktail (HESCO)] containing basic fibroblast growth factor, Wnt3a, April (a proliferation-inducing ligand)/BAFF (B cell-activating factor belonging to TNF), albumin, cholesterol, insulin, and transferrin, which is sufficient for hESC self-renewal and proliferation. Cells grown in HESCO were maintained in an undifferentiated state as determined by using six different stem cell markers, and their genomic integrity was confirmed by karyotyping. Cells cultured in HESCO readily form embryoid bodies in tissue culture and teratomas in mice. In both cases, the cells differentiated into each of the three cell lineages, ectoderm, endoderm, and mesoderm, indicating that they maintained their pluripotency. The use of a minimal medium sufficient for hESC growth is expected to greatly facilitate clinical application and developmental studies of hESCs.</description><subject>B lymphocytes</subject><subject>Biological Sciences</subject><subject>Biomarkers - analysis</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Division - drug effects</subject><subject>Cell lines</subject><subject>Cellular biology</subject><subject>Cellular differentiation</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Cytokines - pharmacology</subject><subject>Embryo, Mammalian</subject><subject>Embryonic stem cells</subject><subject>Embryos</subject><subject>Feeder cells</subject><subject>Human growth</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Neural stem cells</subject><subject>Pluripotent stem cells</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - drug effects</subject><subject>Stem cells</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1v1DAQxS0EotuWc0-giENvaWcytje-IKHQD6RKHChny3GcklViL3aC1P8er3bVD05zmN-8mTePsTOEC4Q1XW69SRcgAakmBHrDVggKS8kVvGUrgGpd1rziR-w4pQ0AKFHDe3aEUighK75i9TfXD951RbOM8xJd0QTfDfMQfCpCX9wuk_HF1dTGx-AHW_yc3VQ0bhzTKXvXmzG5D4d6wn5dX903t-Xdj5vvzde70grF57LOW1Sr6hqFEdS5SlQtdwKxE9iSINGafJ9FI9WaeimtcQQCHLRonSWgE_Zlr7td2sl11vk5mlFv4zCZ-KiDGfTrjh9-64fwVyMXtSSeBc4PAjH8WVya9TQkmy0Y78KSNCpOREJm8PN_4CYs0WdzusoPlgrETu1yD9kYUoquf7oEQe8i0btI9HMkeeLTSwPP_CGDDHzcA5s0h_jUJ8h_QIn0D1-RkAM</recordid><startdate>20060411</startdate><enddate>20060411</enddate><creator>Lu, Jean</creator><creator>Hou, Runhua</creator><creator>Booth, Carmen Jane</creator><creator>Yang, Shih-Hung</creator><creator>Snyder, Michael</creator><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>5PM</scope></search><sort><creationdate>20060411</creationdate><title>Defined Culture Conditions of Human Embryonic Stem Cells</title><author>Lu, Jean ; Hou, Runhua ; Booth, Carmen Jane ; Yang, Shih-Hung ; Snyder, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c594t-86249b98815a53de252b4e511d51b3535ba424c1a6973f66cae3050e0b1cec303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>B lymphocytes</topic><topic>Biological Sciences</topic><topic>Biomarkers - analysis</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Division - drug effects</topic><topic>Cell lines</topic><topic>Cellular biology</topic><topic>Cellular differentiation</topic><topic>Culture Media</topic><topic>Cultured cells</topic><topic>Cytokines - pharmacology</topic><topic>Embryo, Mammalian</topic><topic>Embryonic stem cells</topic><topic>Embryos</topic><topic>Feeder cells</topic><topic>Human growth</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Neural stem cells</topic><topic>Pluripotent stem cells</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - drug effects</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Jean</creatorcontrib><creatorcontrib>Hou, Runhua</creatorcontrib><creatorcontrib>Booth, Carmen Jane</creatorcontrib><creatorcontrib>Yang, Shih-Hung</creatorcontrib><creatorcontrib>Snyder, Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Jean</au><au>Hou, Runhua</au><au>Booth, Carmen Jane</au><au>Yang, Shih-Hung</au><au>Snyder, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defined Culture Conditions of Human Embryonic Stem Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2006-04-11</date><risdate>2006</risdate><volume>103</volume><issue>15</issue><spage>5688</spage><epage>5693</epage><pages>5688-5693</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by the difficulties of eliminating animal products in the culture medium and/or the complexity of conditions required to support hESC growth. We have developed a simple medium [termed hESC Cocktail (HESCO)] containing basic fibroblast growth factor, Wnt3a, April (a proliferation-inducing ligand)/BAFF (B cell-activating factor belonging to TNF), albumin, cholesterol, insulin, and transferrin, which is sufficient for hESC self-renewal and proliferation. Cells grown in HESCO were maintained in an undifferentiated state as determined by using six different stem cell markers, and their genomic integrity was confirmed by karyotyping. Cells cultured in HESCO readily form embryoid bodies in tissue culture and teratomas in mice. In both cases, the cells differentiated into each of the three cell lineages, ectoderm, endoderm, and mesoderm, indicating that they maintained their pluripotency. The use of a minimal medium sufficient for hESC growth is expected to greatly facilitate clinical application and developmental studies of hESCs.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>16595624</pmid><doi>10.1073/pnas.0601383103</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2006-04, Vol.103 (15), p.5688-5693 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1458634 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | B lymphocytes Biological Sciences Biomarkers - analysis Biotechnology Cell Culture Techniques - methods Cell Division - drug effects Cell lines Cellular biology Cellular differentiation Culture Media Cultured cells Cytokines - pharmacology Embryo, Mammalian Embryonic stem cells Embryos Feeder cells Human growth Humans Karyotyping Neural stem cells Pluripotent stem cells Pluripotent Stem Cells - cytology Pluripotent Stem Cells - drug effects Stem cells |
| title | Defined Culture Conditions of Human Embryonic Stem Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T12%3A21%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Defined%20Culture%20Conditions%20of%20Human%20Embryonic%20Stem%20Cells&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Lu,%20Jean&rft.date=2006-04-11&rft.volume=103&rft.issue=15&rft.spage=5688&rft.epage=5693&rft.pages=5688-5693&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0601383103&rft_dat=%3Cjstor_pubme%3E30050161%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201369054&rft_id=info:pmid/16595624&rft_jstor_id=30050161&rfr_iscdi=true |