Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR

We report a novel method for rapid quantification of the degree of DNA methylation of a specific gene. Our method combined bisulfite-mediated PCR and quantification of deoxyribonucleoside monophosphate (dNMP) contents in the PCR product through capillary electrophoresis. A specific bisulfite-PCR pro...

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Veröffentlicht in:	Nucleic acids research 2006-01, Vol.34 (8), p.e61-e61
Hauptverfasser:	Yang, Inchul, Park, In Young, Jang, Sung-Moon, Shi, Lian Hua, Ku, Hyung-Keun, Park, Sang-Ryoul
Format:	Artikel
Sprache:	eng
Schlagworte:	Cytosine - analysis Deoxyribonucleotides - analysis Deoxyribonucleotides - chemistry Deoxyribonucleotides - standards DNA Methylation Electrophoresis, Capillary Hydrolysis Methods Online Polymerase Chain Reaction - methods Reference Standards Sulfites
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