Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II
A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage‐specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In res...
Gespeichert in:
| Veröffentlicht in: | Immunology 1997-04, Vol.90 (4), p.470-476 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 476 |
|---|---|
| container_issue | 4 |
| container_start_page | 470 |
| container_title | Immunology |
| container_volume | 90 |
| creator | DIALYNAS, D. P. LEE, M.‐J. SHAO, L.‐E. TAN, P. C. YU, J. |
| description | A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage‐specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In response to ligation of HLA class II (HLA‐DR), but not in response to ligation of HLA class I, K1m forms tighter homotypic aggregates and develops a striking ‘stellate’ culture phenotype. K1m also expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and CD16) and can be shown to phagocytose polystyrene latex beads, as well as neuroblastoma cells in the presence of tumour‐specific monoclonal antibody (mAb). The K1m cell line should therefore prove useful for studying both signalling through macrophage HLA class II and immunophagocytosis. |
| doi_str_mv | 10.1046/j.1365-2567.1997.00185.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1456704</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79047761</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4995-f2f5b6a7f7f365d2df76d5b9d586df950d35157132a849686f799d10d1e8a3983</originalsourceid><addsrcrecordid>eNqNUk1v1DAQtRBVWQo_Acknbgl2EtuxhJCqCuiKreAAZ8vrj8SrxF7ipG34MfxWnN3Vip7gZHvemzfjeQMAxCjHqKLvdjkuKckKQlmOOWc5Qrgm-eMzsDoDz8EqRXlW1Ii8AC9j3KVniQi5BJccM4o4W4Hf31rjwzjvnYLSa2gnr0YXvOygauUg1WgG90suIRgslNCbB9hOvfSwl2oI-1Y2BirTdbBz3sAvuIfa9MHHcUhZvoGu7yd_4AU1j0uZVODejfOhXnRNqtUtxLEdwtS08HZzDVUnY4Tr9StwYWUXzevTeQV-fPr4_eY223z9vL653mSq4pxktrBkSyWzzKbf60JbRjXZck1qqi0nSJcEE4bLQtYVpzW1jHONkcamliWvyyvw4ai7n7a90cr41H4n9oPr5TCLIJ14injXiibcC1ylSaMqCbw9CQzh52TiKHoXl7FIb8IUBeOoYozifxIxRUUyZ2mpPhLTlGMcjD13g5FYlkDsxOK1WLwWyxKIwxKIx5T65u_fnBNPrif8_RF_cJ2Z_1tXrO_u0qX8A_QHxLE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16021768</pqid></control><display><type>article</type><title>Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><creator>DIALYNAS, D. P. ; LEE, M.‐J. ; SHAO, L.‐E. ; TAN, P. C. ; YU, J.</creator><creatorcontrib>DIALYNAS, D. P. ; LEE, M.‐J. ; SHAO, L.‐E. ; TAN, P. C. ; YU, J.</creatorcontrib><description>A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage‐specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In response to ligation of HLA class II (HLA‐DR), but not in response to ligation of HLA class I, K1m forms tighter homotypic aggregates and develops a striking ‘stellate’ culture phenotype. K1m also expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and CD16) and can be shown to phagocytose polystyrene latex beads, as well as neuroblastoma cells in the presence of tumour‐specific monoclonal antibody (mAb). The K1m cell line should therefore prove useful for studying both signalling through macrophage HLA class II and immunophagocytosis.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1046/j.1365-2567.1997.00185.x</identifier><identifier>PMID: 9176097</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Antibody-Dependent Cell Cytotoxicity ; Antigens, Surface - analysis ; Cell Line - immunology ; Flow Cytometry ; HLA-DR Antigens - immunology ; Humans ; Immunophenotyping ; Macrophage Activation - immunology ; Macrophages - immunology ; Phagocytosis - immunology ; Signal Transduction - immunology</subject><ispartof>Immunology, 1997-04, Vol.90 (4), p.470-476</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4995-f2f5b6a7f7f365d2df76d5b9d586df950d35157132a849686f799d10d1e8a3983</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1456704/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1456704/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,1419,1435,27931,27932,45581,45582,46416,46840,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9176097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DIALYNAS, D. P.</creatorcontrib><creatorcontrib>LEE, M.‐J.</creatorcontrib><creatorcontrib>SHAO, L.‐E.</creatorcontrib><creatorcontrib>TAN, P. C.</creatorcontrib><creatorcontrib>YU, J.</creatorcontrib><title>Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II</title><title>Immunology</title><addtitle>Immunology</addtitle><description>A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage‐specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In response to ligation of HLA class II (HLA‐DR), but not in response to ligation of HLA class I, K1m forms tighter homotypic aggregates and develops a striking ‘stellate’ culture phenotype. K1m also expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and CD16) and can be shown to phagocytose polystyrene latex beads, as well as neuroblastoma cells in the presence of tumour‐specific monoclonal antibody (mAb). The K1m cell line should therefore prove useful for studying both signalling through macrophage HLA class II and immunophagocytosis.</description><subject>Antibody-Dependent Cell Cytotoxicity</subject><subject>Antigens, Surface - analysis</subject><subject>Cell Line - immunology</subject><subject>Flow Cytometry</subject><subject>HLA-DR Antigens - immunology</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Macrophage Activation - immunology</subject><subject>Macrophages - immunology</subject><subject>Phagocytosis - immunology</subject><subject>Signal Transduction - immunology</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUk1v1DAQtRBVWQo_Acknbgl2EtuxhJCqCuiKreAAZ8vrj8SrxF7ipG34MfxWnN3Vip7gZHvemzfjeQMAxCjHqKLvdjkuKckKQlmOOWc5Qrgm-eMzsDoDz8EqRXlW1Ii8AC9j3KVniQi5BJccM4o4W4Hf31rjwzjvnYLSa2gnr0YXvOygauUg1WgG90suIRgslNCbB9hOvfSwl2oI-1Y2BirTdbBz3sAvuIfa9MHHcUhZvoGu7yd_4AU1j0uZVODejfOhXnRNqtUtxLEdwtS08HZzDVUnY4Tr9StwYWUXzevTeQV-fPr4_eY223z9vL653mSq4pxktrBkSyWzzKbf60JbRjXZck1qqi0nSJcEE4bLQtYVpzW1jHONkcamliWvyyvw4ai7n7a90cr41H4n9oPr5TCLIJ14injXiibcC1ylSaMqCbw9CQzh52TiKHoXl7FIb8IUBeOoYozifxIxRUUyZ2mpPhLTlGMcjD13g5FYlkDsxOK1WLwWyxKIwxKIx5T65u_fnBNPrif8_RF_cJ2Z_1tXrO_u0qX8A_QHxLE</recordid><startdate>199704</startdate><enddate>199704</enddate><creator>DIALYNAS, D. P.</creator><creator>LEE, M.‐J.</creator><creator>SHAO, L.‐E.</creator><creator>TAN, P. C.</creator><creator>YU, J.</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199704</creationdate><title>Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II</title><author>DIALYNAS, D. P. ; LEE, M.‐J. ; SHAO, L.‐E. ; TAN, P. C. ; YU, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4995-f2f5b6a7f7f365d2df76d5b9d586df950d35157132a849686f799d10d1e8a3983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Antibody-Dependent Cell Cytotoxicity</topic><topic>Antigens, Surface - analysis</topic><topic>Cell Line - immunology</topic><topic>Flow Cytometry</topic><topic>HLA-DR Antigens - immunology</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Macrophage Activation - immunology</topic><topic>Macrophages - immunology</topic><topic>Phagocytosis - immunology</topic><topic>Signal Transduction - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DIALYNAS, D. P.</creatorcontrib><creatorcontrib>LEE, M.‐J.</creatorcontrib><creatorcontrib>SHAO, L.‐E.</creatorcontrib><creatorcontrib>TAN, P. C.</creatorcontrib><creatorcontrib>YU, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DIALYNAS, D. P.</au><au>LEE, M.‐J.</au><au>SHAO, L.‐E.</au><au>TAN, P. C.</au><au>YU, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>1997-04</date><risdate>1997</risdate><volume>90</volume><issue>4</issue><spage>470</spage><epage>476</epage><pages>470-476</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><abstract>A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage‐specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In response to ligation of HLA class II (HLA‐DR), but not in response to ligation of HLA class I, K1m forms tighter homotypic aggregates and develops a striking ‘stellate’ culture phenotype. K1m also expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and CD16) and can be shown to phagocytose polystyrene latex beads, as well as neuroblastoma cells in the presence of tumour‐specific monoclonal antibody (mAb). The K1m cell line should therefore prove useful for studying both signalling through macrophage HLA class II and immunophagocytosis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9176097</pmid><doi>10.1046/j.1365-2567.1997.00185.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0019-2805 |
| ispartof | Immunology, 1997-04, Vol.90 (4), p.470-476 |
| issn | 0019-2805 1365-2567 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1456704 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | Antibody-Dependent Cell Cytotoxicity Antigens, Surface - analysis Cell Line - immunology Flow Cytometry HLA-DR Antigens - immunology Humans Immunophenotyping Macrophage Activation - immunology Macrophages - immunology Phagocytosis - immunology Signal Transduction - immunology |
| title | Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-07T16%3A59%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phenotypic%20and%20functional%20characterization%20of%20a%20new%20human%20macrophage%20cell%20line%20K1m%20demonstrating%20immunophagocytic%20activity%20and%20signalling%20through%20HLA%20class%20II&rft.jtitle=Immunology&rft.au=DIALYNAS,%20D.%20P.&rft.date=1997-04&rft.volume=90&rft.issue=4&rft.spage=470&rft.epage=476&rft.pages=470-476&rft.issn=0019-2805&rft.eissn=1365-2567&rft_id=info:doi/10.1046/j.1365-2567.1997.00185.x&rft_dat=%3Cproquest_pubme%3E79047761%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16021768&rft_id=info:pmid/9176097&rfr_iscdi=true |