Photocleavable Biotin Phosphoramidite for 5′-End-Labeling, Affinity Purification and Phosphorylation of Synthetic Oligonucleotides

We report the design, synthesis and evaluation of a non-nucleosidic photocleavable biotin phosphoramidite (PCB-phosphoramidite) which provides a simple method for purification and phosphorylation of oligonucleotides. This reagent introduces a photocleavable biotin label (PCB) on the 5′-terminal phos...

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Veröffentlicht in:	Nucleic acids research 1996-01, Vol.24 (2), p.361-366
Hauptverfasser:	Olejnik, Jerzy, Krzymanska-Olejnik, Edyta, Rothschild, Kenneth J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotin - analogs & derivatives Biotin - chemical synthesis Biotin - chemistry Oligonucleotides - chemical synthesis Oligonucleotides - chemistry Oligonucleotides - isolation & purification Oligonucleotides - metabolism Organophosphorus Compounds - chemical synthesis Organophosphorus Compounds - chemistry Phosphorylation Photolysis Ultraviolet Rays
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