Cloning of cDNA for the bovine IL-2 receptor (bovine Tac antigen)
We have cloned the Tac analog of the bovine IL-2 receptor (IL-2R) cDNA. Using mouse and human cDNA probes, we isolated five bovine IL-2R clones from a lambda gt11 bovine long-term lymphocyte cDNA library. Three of the clones had inserts of 2600 base pairs (bp), the same size as the bovine IL-2R mRNA...
Gespeichert in:
| Veröffentlicht in: | Immunology 1988-04, Vol.63 (4), p.603-610 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 610 |
|---|---|
| container_issue | 4 |
| container_start_page | 603 |
| container_title | Immunology |
| container_volume | 63 |
| creator | WEINBERG, A. D SHAW, J PAETKAU, V BLEACKLEY, R. C MAGNUSON, N. S REEVES, R MAGNUSON, J. A |
| description | We have cloned the Tac analog of the bovine IL-2 receptor (IL-2R) cDNA. Using mouse and human cDNA probes, we isolated five bovine IL-2R clones from a lambda gt11 bovine long-term lymphocyte cDNA library. Three of the clones had inserts of 2600 base pairs (bp), the same size as the bovine IL-2R mRNA visualized on Northern blots. The full-length cDNA contain a 190-bp 5' untranslated region, followed by a 825-bp coding region, and a 3' untranslated region that contain 1600 bp. Comparison of the bovine and human IL-2R-coding sequences revealed 71% homology at the nucleotide level. The 3' and 5' non-coding regions were not as homologous, apart from a specific site in the 5'-untranslated region that contained a 5'-upstream start codon. In this region, 24 of 26 nucleotides were identical for the human and bovine cDNAs. Further analysis of the bovine IL-2R sequence also revealed the following: (i) the hydrophobic domains of the IL-2R protein were more conserved between species than the hydrophilic domains, (ii) the predominant site of intracellular IL-2R phosphorylation in mouse and human was a conserved Ser which was not conserved in the bovine sequence, and (iii) there exists a statistically significant amino acid homology with the AIDS gag protein. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1454810</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14925259</sourcerecordid><originalsourceid>FETCH-LOGICAL-p322t-f05eb3f1e6905c63e4590bd9162b7c680ec0f3d69df0bb57bd302da5c2fa0ddc3</originalsourceid><addsrcrecordid>eNqFkMtKw0AUhgdRaq0-gjAbRReBueQkmY1Q6q1QdFPXYa7tSDoTM2nBtzdgEF25Opzzffz8nCM0pbyAjEFRHqMpIVRkrCJwis5Seh9WTgAmaMIqDpzSKZovmhh82ODosL5_mWMXO9xvLVbx4IPFy1XGcGe1bfsB3IzXtdRYht5vbLg9RydONslejHOG3h4f1ovnbPX6tFzMV1nLGeszR8Aq7qgtBAFdcJuDIMoIWjBV6qIiVhPHTSGMI0pBqQwnzEjQzElijOYzdPed2-7VzhptQ9_Jpm47v5PdZx2lr_-S4Lf1Jh5qmkNeUTIEXI8BXfzY29TXO5-0bRoZbNynuqwYqaqc_ivSXDBgIAbx8nelny7jdwd-NXKZtGxcJ4P26UejAqAsS8a_AC56g1s</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14925259</pqid></control><display><type>article</type><title>Cloning of cDNA for the bovine IL-2 receptor (bovine Tac antigen)</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>WEINBERG, A. D ; SHAW, J ; PAETKAU, V ; BLEACKLEY, R. C ; MAGNUSON, N. S ; REEVES, R ; MAGNUSON, J. A</creator><creatorcontrib>WEINBERG, A. D ; SHAW, J ; PAETKAU, V ; BLEACKLEY, R. C ; MAGNUSON, N. S ; REEVES, R ; MAGNUSON, J. A</creatorcontrib><description>We have cloned the Tac analog of the bovine IL-2 receptor (IL-2R) cDNA. Using mouse and human cDNA probes, we isolated five bovine IL-2R clones from a lambda gt11 bovine long-term lymphocyte cDNA library. Three of the clones had inserts of 2600 base pairs (bp), the same size as the bovine IL-2R mRNA visualized on Northern blots. The full-length cDNA contain a 190-bp 5' untranslated region, followed by a 825-bp coding region, and a 3' untranslated region that contain 1600 bp. Comparison of the bovine and human IL-2R-coding sequences revealed 71% homology at the nucleotide level. The 3' and 5' non-coding regions were not as homologous, apart from a specific site in the 5'-untranslated region that contained a 5'-upstream start codon. In this region, 24 of 26 nucleotides were identical for the human and bovine cDNAs. Further analysis of the bovine IL-2R sequence also revealed the following: (i) the hydrophobic domains of the IL-2R protein were more conserved between species than the hydrophilic domains, (ii) the predominant site of intracellular IL-2R phosphorylation in mouse and human was a conserved Ser which was not conserved in the bovine sequence, and (iii) there exists a statistically significant amino acid homology with the AIDS gag protein.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>PMID: 2835311</identifier><identifier>CODEN: IMMUAM</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Amino Acid Sequence ; Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antigens, Surface - genetics ; Base Sequence ; Biological and medical sciences ; Cattle - immunology ; Cloning, Molecular ; DNA - analysis ; Electrophoresis, Agar Gel ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunobiology ; Lymphokines, interleukins ( function, expression) ; Molecular Sequence Data ; Receptors, Immunologic - genetics ; Receptors, Interleukin-2 ; Regulatory factors and their cellular receptors ; Tumor Necrosis Factor Receptor Superfamily, Member 7</subject><ispartof>Immunology, 1988-04, Vol.63 (4), p.603-610</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1454810/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1454810/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19557772$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2835311$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WEINBERG, A. D</creatorcontrib><creatorcontrib>SHAW, J</creatorcontrib><creatorcontrib>PAETKAU, V</creatorcontrib><creatorcontrib>BLEACKLEY, R. C</creatorcontrib><creatorcontrib>MAGNUSON, N. S</creatorcontrib><creatorcontrib>REEVES, R</creatorcontrib><creatorcontrib>MAGNUSON, J. A</creatorcontrib><title>Cloning of cDNA for the bovine IL-2 receptor (bovine Tac antigen)</title><title>Immunology</title><addtitle>Immunology</addtitle><description>We have cloned the Tac analog of the bovine IL-2 receptor (IL-2R) cDNA. Using mouse and human cDNA probes, we isolated five bovine IL-2R clones from a lambda gt11 bovine long-term lymphocyte cDNA library. Three of the clones had inserts of 2600 base pairs (bp), the same size as the bovine IL-2R mRNA visualized on Northern blots. The full-length cDNA contain a 190-bp 5' untranslated region, followed by a 825-bp coding region, and a 3' untranslated region that contain 1600 bp. Comparison of the bovine and human IL-2R-coding sequences revealed 71% homology at the nucleotide level. The 3' and 5' non-coding regions were not as homologous, apart from a specific site in the 5'-untranslated region that contained a 5'-upstream start codon. In this region, 24 of 26 nucleotides were identical for the human and bovine cDNAs. Further analysis of the bovine IL-2R sequence also revealed the following: (i) the hydrophobic domains of the IL-2R protein were more conserved between species than the hydrophilic domains, (ii) the predominant site of intracellular IL-2R phosphorylation in mouse and human was a conserved Ser which was not conserved in the bovine sequence, and (iii) there exists a statistically significant amino acid homology with the AIDS gag protein.</description><subject>Amino Acid Sequence</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antigens, Surface - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cattle - immunology</subject><subject>Cloning, Molecular</subject><subject>DNA - analysis</subject><subject>Electrophoresis, Agar Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Molecular Sequence Data</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, Interleukin-2</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 7</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKw0AUhgdRaq0-gjAbRReBueQkmY1Q6q1QdFPXYa7tSDoTM2nBtzdgEF25Opzzffz8nCM0pbyAjEFRHqMpIVRkrCJwis5Seh9WTgAmaMIqDpzSKZovmhh82ODosL5_mWMXO9xvLVbx4IPFy1XGcGe1bfsB3IzXtdRYht5vbLg9RydONslejHOG3h4f1ovnbPX6tFzMV1nLGeszR8Aq7qgtBAFdcJuDIMoIWjBV6qIiVhPHTSGMI0pBqQwnzEjQzElijOYzdPed2-7VzhptQ9_Jpm47v5PdZx2lr_-S4Lf1Jh5qmkNeUTIEXI8BXfzY29TXO5-0bRoZbNynuqwYqaqc_ivSXDBgIAbx8nelny7jdwd-NXKZtGxcJ4P26UejAqAsS8a_AC56g1s</recordid><startdate>19880401</startdate><enddate>19880401</enddate><creator>WEINBERG, A. D</creator><creator>SHAW, J</creator><creator>PAETKAU, V</creator><creator>BLEACKLEY, R. C</creator><creator>MAGNUSON, N. S</creator><creator>REEVES, R</creator><creator>MAGNUSON, J. A</creator><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19880401</creationdate><title>Cloning of cDNA for the bovine IL-2 receptor (bovine Tac antigen)</title><author>WEINBERG, A. D ; SHAW, J ; PAETKAU, V ; BLEACKLEY, R. C ; MAGNUSON, N. S ; REEVES, R ; MAGNUSON, J. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p322t-f05eb3f1e6905c63e4590bd9162b7c680ec0f3d69df0bb57bd302da5c2fa0ddc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antigens, Surface - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cattle - immunology</topic><topic>Cloning, Molecular</topic><topic>DNA - analysis</topic><topic>Electrophoresis, Agar Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Molecular Sequence Data</topic><topic>Receptors, Immunologic - genetics</topic><topic>Receptors, Interleukin-2</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Tumor Necrosis Factor Receptor Superfamily, Member 7</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WEINBERG, A. D</creatorcontrib><creatorcontrib>SHAW, J</creatorcontrib><creatorcontrib>PAETKAU, V</creatorcontrib><creatorcontrib>BLEACKLEY, R. C</creatorcontrib><creatorcontrib>MAGNUSON, N. S</creatorcontrib><creatorcontrib>REEVES, R</creatorcontrib><creatorcontrib>MAGNUSON, J. A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WEINBERG, A. D</au><au>SHAW, J</au><au>PAETKAU, V</au><au>BLEACKLEY, R. C</au><au>MAGNUSON, N. S</au><au>REEVES, R</au><au>MAGNUSON, J. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of cDNA for the bovine IL-2 receptor (bovine Tac antigen)</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>1988-04-01</date><risdate>1988</risdate><volume>63</volume><issue>4</issue><spage>603</spage><epage>610</epage><pages>603-610</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><coden>IMMUAM</coden><abstract>We have cloned the Tac analog of the bovine IL-2 receptor (IL-2R) cDNA. Using mouse and human cDNA probes, we isolated five bovine IL-2R clones from a lambda gt11 bovine long-term lymphocyte cDNA library. Three of the clones had inserts of 2600 base pairs (bp), the same size as the bovine IL-2R mRNA visualized on Northern blots. The full-length cDNA contain a 190-bp 5' untranslated region, followed by a 825-bp coding region, and a 3' untranslated region that contain 1600 bp. Comparison of the bovine and human IL-2R-coding sequences revealed 71% homology at the nucleotide level. The 3' and 5' non-coding regions were not as homologous, apart from a specific site in the 5'-untranslated region that contained a 5'-upstream start codon. In this region, 24 of 26 nucleotides were identical for the human and bovine cDNAs. Further analysis of the bovine IL-2R sequence also revealed the following: (i) the hydrophobic domains of the IL-2R protein were more conserved between species than the hydrophilic domains, (ii) the predominant site of intracellular IL-2R phosphorylation in mouse and human was a conserved Ser which was not conserved in the bovine sequence, and (iii) there exists a statistically significant amino acid homology with the AIDS gag protein.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>2835311</pmid><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0019-2805 |
| ispartof | Immunology, 1988-04, Vol.63 (4), p.603-610 |
| issn | 0019-2805 1365-2567 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1454810 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Amino Acid Sequence Analysis of the immune response. Humoral and cellular immunity Animals Antigens, Surface - genetics Base Sequence Biological and medical sciences Cattle - immunology Cloning, Molecular DNA - analysis Electrophoresis, Agar Gel Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology Lymphokines, interleukins ( function, expression) Molecular Sequence Data Receptors, Immunologic - genetics Receptors, Interleukin-2 Regulatory factors and their cellular receptors Tumor Necrosis Factor Receptor Superfamily, Member 7 |
| title | Cloning of cDNA for the bovine IL-2 receptor (bovine Tac antigen) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T17%3A19%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20of%20cDNA%20for%20the%20bovine%20IL-2%20receptor%20(bovine%20Tac%20antigen)&rft.jtitle=Immunology&rft.au=WEINBERG,%20A.%20D&rft.date=1988-04-01&rft.volume=63&rft.issue=4&rft.spage=603&rft.epage=610&rft.pages=603-610&rft.issn=0019-2805&rft.eissn=1365-2567&rft.coden=IMMUAM&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E14925259%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14925259&rft_id=info:pmid/2835311&rfr_iscdi=true |