Rac3-induced neuritogenesis requires binding to Neurabin I
Rac3, a neuronal GTP-binding protein of the Rho family, induces neuritogenesis in primary neurons. Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I...
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| Veröffentlicht in: | Molecular biology of the cell 2006-05, Vol.17 (5), p.2391-2400 |
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| creator | Orioli, Donata Colaluca, Ivan N Stefanini, Miria Riva, Silvano Dotti, Carlos G Peverali, Fiorenzo A |
| description | Rac3, a neuronal GTP-binding protein of the Rho family, induces neuritogenesis in primary neurons. Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. These results show that Neurabin I mediates Rac3-induced neuritogenesis, possibly by anchoring Rac3 to growth cone F-actin. |
| doi_str_mv | 10.1091/mbc.e05-08-0753 |
| format | Article |
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Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. 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Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. These results show that Neurabin I mediates Rac3-induced neuritogenesis, possibly by anchoring Rac3 to growth cone F-actin.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cytoskeleton - chemistry</subject><subject>Cytoskeleton - metabolism</subject><subject>Growth Cones - chemistry</subject><subject>Growth Cones - metabolism</subject><subject>Growth Cones - physiology</subject><subject>Immunoprecipitation</subject><subject>Mice</subject><subject>Microfilament Proteins - analysis</subject><subject>Microfilament Proteins - genetics</subject><subject>Microfilament Proteins - metabolism</subject><subject>Mutation</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurites - chemistry</subject><subject>Neurites - physiology</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Neurons - physiology</subject><subject>Nuclear Receptor Coactivator 3</subject><subject>Oligodeoxyribonucleotides, Antisense - genetics</subject><subject>Oligodeoxyribonucleotides, Antisense - pharmacology</subject><subject>Protein Interaction Mapping</subject><subject>Protein Structure, Tertiary</subject><subject>Rats</subject><subject>Transcription Factors - analysis</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptional Activation</subject><issn>1059-1524</issn><issn>1939-4586</issn><issn>1059-1524</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLxDAQh4Movs_epCdv1Zk0kzYeBBFfIAriPaTtdI3stpq0gv-9WVx8nDwlYb78mJlPiAOEYwSDJ4u6OWagHKocSirWxDaawuSKKr2e7kAmR5JqS-zE-AKASulyU2yhJkkgaVucPrqmyH3fTg23Wc9T8OMw456jj1ngt8kHjlmdAN_PsnHI7hPi0ju73RMbnZtH3l-du-Lp6vLp4ia_e7i-vTi_yxvSNOZcGImdJtSyq1uNsoTWSGmQCcF1DWiCjqnQoLCkWhW1qZw2VaUMddIVu-LsK_Z1qhfcNtyPwc3ta_ALFz7s4Lz9W-n9s50N73Y5LJQqBRytAsLwNnEc7cLHhudz1_MwRavLyqTdwb8gGiJEJRN48gU2YYgxcPfdDYJderHJi01eLFR26SX9OPw9xA-_ElF8Av0SiNY</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Orioli, Donata</creator><creator>Colaluca, Ivan N</creator><creator>Stefanini, Miria</creator><creator>Riva, Silvano</creator><creator>Dotti, Carlos G</creator><creator>Peverali, Fiorenzo A</creator><general>The American Society for Cell Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200605</creationdate><title>Rac3-induced neuritogenesis requires binding to Neurabin I</title><author>Orioli, Donata ; 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Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. These results show that Neurabin I mediates Rac3-induced neuritogenesis, possibly by anchoring Rac3 to growth cone F-actin.</abstract><cop>United States</cop><pub>The American Society for Cell Biology</pub><pmid>16525025</pmid><doi>10.1091/mbc.e05-08-0753</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actins - metabolism Animals Cells, Cultured Cytoskeleton - chemistry Cytoskeleton - metabolism Growth Cones - chemistry Growth Cones - metabolism Growth Cones - physiology Immunoprecipitation Mice Microfilament Proteins - analysis Microfilament Proteins - genetics Microfilament Proteins - metabolism Mutation Nerve Tissue Proteins - analysis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neurites - chemistry Neurites - physiology Neurons - cytology Neurons - metabolism Neurons - physiology Nuclear Receptor Coactivator 3 Oligodeoxyribonucleotides, Antisense - genetics Oligodeoxyribonucleotides, Antisense - pharmacology Protein Interaction Mapping Protein Structure, Tertiary Rats Transcription Factors - analysis Transcription Factors - genetics Transcription Factors - metabolism Transcriptional Activation |
| title | Rac3-induced neuritogenesis requires binding to Neurabin I |
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