Effect of alcohols on gastric and small intestinal apical membrane integrity and fluidity
Duodenal and jejunal brush border membrane vesicle integrity was studied after in vitro treatment of rabbit tissue with ethyl, benzyl or octyl alcohol. The effects of the alcohols on gastric parietal cell apical and microsomal membrane vesicle integrity was also studied. Membrane vesicle integrity w...
Gespeichert in:
| Veröffentlicht in: | Gut 1988-12, Vol.29 (12), p.1648-1655 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1655 |
|---|---|
| container_issue | 12 |
| container_start_page | 1648 |
| container_title | Gut |
| container_volume | 29 |
| creator | Ballard, H J Wilkes, J M Hirst, B H |
| description | Duodenal and jejunal brush border membrane vesicle integrity was studied after in vitro treatment of rabbit tissue with ethyl, benzyl or octyl alcohol. The effects of the alcohols on gastric parietal cell apical and microsomal membrane vesicle integrity was also studied. Membrane vesicle integrity was determined from the enclosed volume of the vesicle preparations, measured as [14C]glucose space at equilibrium. Exposure of vesicles to the three alcohols caused concentration dependent decreases in enclosed volume. The rank order of potency of the alcohol was octyl greater than benzyl greater than ethyl. Concentrations greater than or equal to 10 mM benzyl alcohol significantly reduced the enclosed volume of duodenal or jejunal vesicles; jejunal vesicles were disrupted by 625 mM ethanol, whereas 2 M ethanol was required to disrupt the duodenal vesicles. Gastric apical membrane integrity was reduced with 0.25 M ethanol, the vesicles being approximately an order of magnitude more sensitive to ethanol than gross estimates of gastric mucosal damage, but 1 M ethanol was required to significantly damage gastric microsomes. All concentrations of benzyl or octyl alcohol tested (greater than or equal to 5 mM) reduced the enclosed volume of both gastric apical membrane vesicles and gastric microsomes. As determined by shrink-swell techniques, benzyl alcohol permeated duodenal vesicles at a faster rate than NH4Cl (apparent rate constant of 9.89 (0.71) X 10(-3)s-1 compared with 4.48 (0.23) X 10(-3)s-1). Therefore, reductions in enclosed volume in response to alcohol treatment could not be explained by alcohol induced osmotic shrinkage. The enclosed volume of the vesicles after alcohol treatment was negatively correlated with membrane fluidity suggesting a common causal effect, the increased fluidity increasing membrane fragility. Duodenal vesicles were more resistant to disruption by the alcohols compared with gastric and jejunal vesicles. |
| doi_str_mv | 10.1136/gut.29.12.1648 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1434108</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78643885</sourcerecordid><originalsourceid>FETCH-LOGICAL-b514t-9acadc266c15491b9b038911642cba64cb694632b7ff8296166f08455c18f0f93</originalsourceid><addsrcrecordid>eNqFkc2LFDEQxYMo67h69SY0KIKHblNJOp1cBB1XV1nWg194CulMMpsx3RmTbnH_ezM7w6BePBXh_fKoVw-hh4AbAMqfr-epIbIB0gBn4hZaAOOipkSI22iBMXR12zF5F93LeYMxFkLCCTqhhGCK2QJ9O3POmqmKrtLBxKsYchXHaq3zlLyp9Liq8qBDqPw42Tz5UYdKb70pY7BDn_Rob6R18tP1De7C7FflcR_dcTpk--AwT9HnN2efluf1xYe375YvL-q-BTbVUhu9MoRzAy2T0Mse07JjCUNMrzkzPZeMU9J3zgkiOXDusGBta0A47CQ9RS_2vtu5H-zK2HFKOqht8oNO1ypqr_5WRn-l1vGnAkYZYFEMnh4MUvwxl5Bq8NnYEEq2OGfVCc6oEG0BH_8DbuKcykWygq7DmGPe7eyaPWVSzDlZd1wFsNpVpkplikgFRO0qKx8e_RngiB86KvqTg65zubsrNzc-HzHeCsH4Dqv3mM-T_XWUdfqueEe7Vl1-Warz918v2auPoF4X_tme74fN_1b8DVC3u34</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770060678</pqid></control><display><type>article</type><title>Effect of alcohols on gastric and small intestinal apical membrane integrity and fluidity</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Ballard, H J ; Wilkes, J M ; Hirst, B H</creator><creatorcontrib>Ballard, H J ; Wilkes, J M ; Hirst, B H</creatorcontrib><description>Duodenal and jejunal brush border membrane vesicle integrity was studied after in vitro treatment of rabbit tissue with ethyl, benzyl or octyl alcohol. The effects of the alcohols on gastric parietal cell apical and microsomal membrane vesicle integrity was also studied. Membrane vesicle integrity was determined from the enclosed volume of the vesicle preparations, measured as [14C]glucose space at equilibrium. Exposure of vesicles to the three alcohols caused concentration dependent decreases in enclosed volume. The rank order of potency of the alcohol was octyl greater than benzyl greater than ethyl. Concentrations greater than or equal to 10 mM benzyl alcohol significantly reduced the enclosed volume of duodenal or jejunal vesicles; jejunal vesicles were disrupted by 625 mM ethanol, whereas 2 M ethanol was required to disrupt the duodenal vesicles. Gastric apical membrane integrity was reduced with 0.25 M ethanol, the vesicles being approximately an order of magnitude more sensitive to ethanol than gross estimates of gastric mucosal damage, but 1 M ethanol was required to significantly damage gastric microsomes. All concentrations of benzyl or octyl alcohol tested (greater than or equal to 5 mM) reduced the enclosed volume of both gastric apical membrane vesicles and gastric microsomes. As determined by shrink-swell techniques, benzyl alcohol permeated duodenal vesicles at a faster rate than NH4Cl (apparent rate constant of 9.89 (0.71) X 10(-3)s-1 compared with 4.48 (0.23) X 10(-3)s-1). Therefore, reductions in enclosed volume in response to alcohol treatment could not be explained by alcohol induced osmotic shrinkage. The enclosed volume of the vesicles after alcohol treatment was negatively correlated with membrane fluidity suggesting a common causal effect, the increased fluidity increasing membrane fragility. Duodenal vesicles were more resistant to disruption by the alcohols compared with gastric and jejunal vesicles.</description><identifier>ISSN: 0017-5749</identifier><identifier>EISSN: 1468-3288</identifier><identifier>EISSN: 1458-3288</identifier><identifier>DOI: 10.1136/gut.29.12.1648</identifier><identifier>PMID: 3220304</identifier><identifier>CODEN: GUTTAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and British Society of Gastroenterology</publisher><subject>Alcoholism and acute alcohol poisoning ; Alcohols - pharmacology ; Animals ; Benzyl Alcohols - pharmacology ; Biological and medical sciences ; Duodenum - ultrastructure ; Ethanol - pharmacology ; Gastric Mucosa - drug effects ; Jejunum - ultrastructure ; Medical sciences ; Membrane Fluidity - drug effects ; Microsomes - drug effects ; Microvilli - drug effects ; Octanols - pharmacology ; Parietal Cells, Gastric - drug effects ; Rabbits ; Toxicology</subject><ispartof>Gut, 1988-12, Vol.29 (12), p.1648-1655</ispartof><rights>1990 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD Dec 1988</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b514t-9acadc266c15491b9b038911642cba64cb694632b7ff8296166f08455c18f0f93</citedby><cites>FETCH-LOGICAL-b514t-9acadc266c15491b9b038911642cba64cb694632b7ff8296166f08455c18f0f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1434108/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1434108/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6588464$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3220304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ballard, H J</creatorcontrib><creatorcontrib>Wilkes, J M</creatorcontrib><creatorcontrib>Hirst, B H</creatorcontrib><title>Effect of alcohols on gastric and small intestinal apical membrane integrity and fluidity</title><title>Gut</title><addtitle>Gut</addtitle><description>Duodenal and jejunal brush border membrane vesicle integrity was studied after in vitro treatment of rabbit tissue with ethyl, benzyl or octyl alcohol. The effects of the alcohols on gastric parietal cell apical and microsomal membrane vesicle integrity was also studied. Membrane vesicle integrity was determined from the enclosed volume of the vesicle preparations, measured as [14C]glucose space at equilibrium. Exposure of vesicles to the three alcohols caused concentration dependent decreases in enclosed volume. The rank order of potency of the alcohol was octyl greater than benzyl greater than ethyl. Concentrations greater than or equal to 10 mM benzyl alcohol significantly reduced the enclosed volume of duodenal or jejunal vesicles; jejunal vesicles were disrupted by 625 mM ethanol, whereas 2 M ethanol was required to disrupt the duodenal vesicles. Gastric apical membrane integrity was reduced with 0.25 M ethanol, the vesicles being approximately an order of magnitude more sensitive to ethanol than gross estimates of gastric mucosal damage, but 1 M ethanol was required to significantly damage gastric microsomes. All concentrations of benzyl or octyl alcohol tested (greater than or equal to 5 mM) reduced the enclosed volume of both gastric apical membrane vesicles and gastric microsomes. As determined by shrink-swell techniques, benzyl alcohol permeated duodenal vesicles at a faster rate than NH4Cl (apparent rate constant of 9.89 (0.71) X 10(-3)s-1 compared with 4.48 (0.23) X 10(-3)s-1). Therefore, reductions in enclosed volume in response to alcohol treatment could not be explained by alcohol induced osmotic shrinkage. The enclosed volume of the vesicles after alcohol treatment was negatively correlated with membrane fluidity suggesting a common causal effect, the increased fluidity increasing membrane fragility. Duodenal vesicles were more resistant to disruption by the alcohols compared with gastric and jejunal vesicles.</description><subject>Alcoholism and acute alcohol poisoning</subject><subject>Alcohols - pharmacology</subject><subject>Animals</subject><subject>Benzyl Alcohols - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Duodenum - ultrastructure</subject><subject>Ethanol - pharmacology</subject><subject>Gastric Mucosa - drug effects</subject><subject>Jejunum - ultrastructure</subject><subject>Medical sciences</subject><subject>Membrane Fluidity - drug effects</subject><subject>Microsomes - drug effects</subject><subject>Microvilli - drug effects</subject><subject>Octanols - pharmacology</subject><subject>Parietal Cells, Gastric - drug effects</subject><subject>Rabbits</subject><subject>Toxicology</subject><issn>0017-5749</issn><issn>1468-3288</issn><issn>1458-3288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc2LFDEQxYMo67h69SY0KIKHblNJOp1cBB1XV1nWg194CulMMpsx3RmTbnH_ezM7w6BePBXh_fKoVw-hh4AbAMqfr-epIbIB0gBn4hZaAOOipkSI22iBMXR12zF5F93LeYMxFkLCCTqhhGCK2QJ9O3POmqmKrtLBxKsYchXHaq3zlLyp9Liq8qBDqPw42Tz5UYdKb70pY7BDn_Rob6R18tP1De7C7FflcR_dcTpk--AwT9HnN2efluf1xYe375YvL-q-BTbVUhu9MoRzAy2T0Mse07JjCUNMrzkzPZeMU9J3zgkiOXDusGBta0A47CQ9RS_2vtu5H-zK2HFKOqht8oNO1ypqr_5WRn-l1vGnAkYZYFEMnh4MUvwxl5Bq8NnYEEq2OGfVCc6oEG0BH_8DbuKcykWygq7DmGPe7eyaPWVSzDlZd1wFsNpVpkplikgFRO0qKx8e_RngiB86KvqTg65zubsrNzc-HzHeCsH4Dqv3mM-T_XWUdfqueEe7Vl1-Warz918v2auPoF4X_tme74fN_1b8DVC3u34</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Ballard, H J</creator><creator>Wilkes, J M</creator><creator>Hirst, B H</creator><general>BMJ Publishing Group Ltd and British Society of Gastroenterology</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19881201</creationdate><title>Effect of alcohols on gastric and small intestinal apical membrane integrity and fluidity</title><author>Ballard, H J ; Wilkes, J M ; Hirst, B H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b514t-9acadc266c15491b9b038911642cba64cb694632b7ff8296166f08455c18f0f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Alcoholism and acute alcohol poisoning</topic><topic>Alcohols - pharmacology</topic><topic>Animals</topic><topic>Benzyl Alcohols - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Duodenum - ultrastructure</topic><topic>Ethanol - pharmacology</topic><topic>Gastric Mucosa - drug effects</topic><topic>Jejunum - ultrastructure</topic><topic>Medical sciences</topic><topic>Membrane Fluidity - drug effects</topic><topic>Microsomes - drug effects</topic><topic>Microvilli - drug effects</topic><topic>Octanols - pharmacology</topic><topic>Parietal Cells, Gastric - drug effects</topic><topic>Rabbits</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ballard, H J</creatorcontrib><creatorcontrib>Wilkes, J M</creatorcontrib><creatorcontrib>Hirst, B H</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Gut</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ballard, H J</au><au>Wilkes, J M</au><au>Hirst, B H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of alcohols on gastric and small intestinal apical membrane integrity and fluidity</atitle><jtitle>Gut</jtitle><addtitle>Gut</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>29</volume><issue>12</issue><spage>1648</spage><epage>1655</epage><pages>1648-1655</pages><issn>0017-5749</issn><eissn>1468-3288</eissn><eissn>1458-3288</eissn><coden>GUTTAK</coden><abstract>Duodenal and jejunal brush border membrane vesicle integrity was studied after in vitro treatment of rabbit tissue with ethyl, benzyl or octyl alcohol. The effects of the alcohols on gastric parietal cell apical and microsomal membrane vesicle integrity was also studied. Membrane vesicle integrity was determined from the enclosed volume of the vesicle preparations, measured as [14C]glucose space at equilibrium. Exposure of vesicles to the three alcohols caused concentration dependent decreases in enclosed volume. The rank order of potency of the alcohol was octyl greater than benzyl greater than ethyl. Concentrations greater than or equal to 10 mM benzyl alcohol significantly reduced the enclosed volume of duodenal or jejunal vesicles; jejunal vesicles were disrupted by 625 mM ethanol, whereas 2 M ethanol was required to disrupt the duodenal vesicles. Gastric apical membrane integrity was reduced with 0.25 M ethanol, the vesicles being approximately an order of magnitude more sensitive to ethanol than gross estimates of gastric mucosal damage, but 1 M ethanol was required to significantly damage gastric microsomes. All concentrations of benzyl or octyl alcohol tested (greater than or equal to 5 mM) reduced the enclosed volume of both gastric apical membrane vesicles and gastric microsomes. As determined by shrink-swell techniques, benzyl alcohol permeated duodenal vesicles at a faster rate than NH4Cl (apparent rate constant of 9.89 (0.71) X 10(-3)s-1 compared with 4.48 (0.23) X 10(-3)s-1). Therefore, reductions in enclosed volume in response to alcohol treatment could not be explained by alcohol induced osmotic shrinkage. The enclosed volume of the vesicles after alcohol treatment was negatively correlated with membrane fluidity suggesting a common causal effect, the increased fluidity increasing membrane fragility. Duodenal vesicles were more resistant to disruption by the alcohols compared with gastric and jejunal vesicles.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and British Society of Gastroenterology</pub><pmid>3220304</pmid><doi>10.1136/gut.29.12.1648</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0017-5749 |
| ispartof | Gut, 1988-12, Vol.29 (12), p.1648-1655 |
| issn | 0017-5749 1468-3288 1458-3288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1434108 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Alcoholism and acute alcohol poisoning Alcohols - pharmacology Animals Benzyl Alcohols - pharmacology Biological and medical sciences Duodenum - ultrastructure Ethanol - pharmacology Gastric Mucosa - drug effects Jejunum - ultrastructure Medical sciences Membrane Fluidity - drug effects Microsomes - drug effects Microvilli - drug effects Octanols - pharmacology Parietal Cells, Gastric - drug effects Rabbits Toxicology |
| title | Effect of alcohols on gastric and small intestinal apical membrane integrity and fluidity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T11%3A02%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20alcohols%20on%20gastric%20and%20small%20intestinal%20apical%20membrane%20integrity%20and%20fluidity&rft.jtitle=Gut&rft.au=Ballard,%20H%20J&rft.date=1988-12-01&rft.volume=29&rft.issue=12&rft.spage=1648&rft.epage=1655&rft.pages=1648-1655&rft.issn=0017-5749&rft.eissn=1468-3288&rft.coden=GUTTAK&rft_id=info:doi/10.1136/gut.29.12.1648&rft_dat=%3Cproquest_pubme%3E78643885%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1770060678&rft_id=info:pmid/3220304&rfr_iscdi=true |