Cytotoxic activity of human lymphocytes against differentiated intestinal tumour cell lines
The natural killer (NK) and lymphokine-activated killer (LAK) cytotoxic activity of human peripheral blood lymphocytes (PBL) against various human tumour cell lines from intestinal origin (WIDR, HT29, Caco-2) has been investigated. The differentiated Caco-2 cells were then used as a model to investi...
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| Veröffentlicht in: | Immunology 1993, Vol.78 (1), p.166-169 |
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| container_title | Immunology |
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| creator | ANELLI, R PLACIDO, R SAMBUY, Y BACH, S DI MASSIMO, A COLIZZI, V |
| description | The natural killer (NK) and lymphokine-activated killer (LAK) cytotoxic activity of human peripheral blood lymphocytes (PBL) against various human tumour cell lines from intestinal origin (WIDR, HT29, Caco-2) has been investigated. The differentiated Caco-2 cells were then used as a model to investigate the cytotoxic activity against enterocyte-like target cells. Caco-2 were seeded on polycarbonate filters and maintained in culture for at least 15 days to allow the differentiation and formation of tight junctions. The integrity of tight junctions was assayed by measuring [3H]mannitol flux from apical to basolateral compartment. Cytotoxic analysis showed that both differentiated and undifferentiated Caco-2 cells were similarly susceptible to NK and LAK activity. The capacity of cytotoxic lymphocytes to kill enterocyte-like cells with intact junctional complex may suggest a direct role of cytotoxic lymphocytes in causing intestinal lesions under inflammatory conditions. |
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The differentiated Caco-2 cells were then used as a model to investigate the cytotoxic activity against enterocyte-like target cells. Caco-2 were seeded on polycarbonate filters and maintained in culture for at least 15 days to allow the differentiation and formation of tight junctions. The integrity of tight junctions was assayed by measuring [3H]mannitol flux from apical to basolateral compartment. Cytotoxic analysis showed that both differentiated and undifferentiated Caco-2 cells were similarly susceptible to NK and LAK activity. The capacity of cytotoxic lymphocytes to kill enterocyte-like cells with intact junctional complex may suggest a direct role of cytotoxic lymphocytes in causing intestinal lesions under inflammatory conditions.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>PMID: 8436403</identifier><identifier>CODEN: IMMUAM</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Biological and medical sciences ; Cell Line ; Colonic Neoplasms - immunology ; Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others) ; Cytotoxicity, Immunologic - immunology ; Fundamental and applied biological sciences. 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The differentiated Caco-2 cells were then used as a model to investigate the cytotoxic activity against enterocyte-like target cells. Caco-2 were seeded on polycarbonate filters and maintained in culture for at least 15 days to allow the differentiation and formation of tight junctions. The integrity of tight junctions was assayed by measuring [3H]mannitol flux from apical to basolateral compartment. Cytotoxic analysis showed that both differentiated and undifferentiated Caco-2 cells were similarly susceptible to NK and LAK activity. The capacity of cytotoxic lymphocytes to kill enterocyte-like cells with intact junctional complex may suggest a direct role of cytotoxic lymphocytes in causing intestinal lesions under inflammatory conditions.</description><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Colonic Neoplasms - immunology</subject><subject>Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others)</subject><subject>Cytotoxicity, Immunologic - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Immunological reactions in vitro</subject><subject>Intestines - immunology</subject><subject>Killer Cells, Lymphokine-Activated - immunology</subject><subject>Killer Cells, Natural - immunology</subject><subject>Tumor Cells, Cultured - immunology</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9LxDAUxIMo67r6EYQcxFshaZI2exFk8R8seNGTh_KaJruRNlmbdLHf3ohl0ZOnx-M3DDNzhOaUFSLLRVEeozkhdJnlkohTdBbCe3oZEWKGZpKzghM2R2-rMfroP63CoKLd2zhib_B26MDhdux2W6_GqAOGDVgXIm6sMbrXLlqIusHWJRitgxbHofNDj5VuW9xap8M5OjHQBn0x3QV6vb97WT1m6-eHp9XtOtuxnMaMc0VEzgXhRDKtaQPGNFDnGkpa8DInwkgNkiRAl9rUTS54I5QWtZSFKWq2QDc_vruh7nSjUrge2mrX2w76sfJgq7_E2W218fuK8pyWJU0G15NB7z-G1KfqbPjuAU77IVSlEFIIzv4V0qJIiyflAl3-jnTIMu2e-NXEIShoTQ9O2XCQcZFSyZJ9AWI2kAg</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>ANELLI, R</creator><creator>PLACIDO, R</creator><creator>SAMBUY, Y</creator><creator>BACH, S</creator><creator>DI MASSIMO, A</creator><creator>COLIZZI, V</creator><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1993</creationdate><title>Cytotoxic activity of human lymphocytes against differentiated intestinal tumour cell lines</title><author>ANELLI, R ; PLACIDO, R ; SAMBUY, Y ; BACH, S ; DI MASSIMO, A ; COLIZZI, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p321t-44c0524504083ee1daffdab2ea71647205f8ea801da19efbd254d5ce5b886f6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Colonic Neoplasms - immunology</topic><topic>Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others)</topic><topic>Cytotoxicity, Immunologic - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Immunological reactions in vitro</topic><topic>Intestines - immunology</topic><topic>Killer Cells, Lymphokine-Activated - immunology</topic><topic>Killer Cells, Natural - immunology</topic><topic>Tumor Cells, Cultured - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ANELLI, R</creatorcontrib><creatorcontrib>PLACIDO, R</creatorcontrib><creatorcontrib>SAMBUY, Y</creatorcontrib><creatorcontrib>BACH, S</creatorcontrib><creatorcontrib>DI MASSIMO, A</creatorcontrib><creatorcontrib>COLIZZI, V</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ANELLI, R</au><au>PLACIDO, R</au><au>SAMBUY, Y</au><au>BACH, S</au><au>DI MASSIMO, A</au><au>COLIZZI, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxic activity of human lymphocytes against differentiated intestinal tumour cell lines</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>1993</date><risdate>1993</risdate><volume>78</volume><issue>1</issue><spage>166</spage><epage>169</epage><pages>166-169</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><coden>IMMUAM</coden><abstract>The natural killer (NK) and lymphokine-activated killer (LAK) cytotoxic activity of human peripheral blood lymphocytes (PBL) against various human tumour cell lines from intestinal origin (WIDR, HT29, Caco-2) has been investigated. The differentiated Caco-2 cells were then used as a model to investigate the cytotoxic activity against enterocyte-like target cells. Caco-2 were seeded on polycarbonate filters and maintained in culture for at least 15 days to allow the differentiation and formation of tight junctions. The integrity of tight junctions was assayed by measuring [3H]mannitol flux from apical to basolateral compartment. Cytotoxic analysis showed that both differentiated and undifferentiated Caco-2 cells were similarly susceptible to NK and LAK activity. The capacity of cytotoxic lymphocytes to kill enterocyte-like cells with intact junctional complex may suggest a direct role of cytotoxic lymphocytes in causing intestinal lesions under inflammatory conditions.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>8436403</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Immunology, 1993, Vol.78 (1), p.166-169 |
| issn | 0019-2805 1365-2567 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biological and medical sciences Cell Line Colonic Neoplasms - immunology Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others) Cytotoxicity, Immunologic - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Immunological reactions in vitro Intestines - immunology Killer Cells, Lymphokine-Activated - immunology Killer Cells, Natural - immunology Tumor Cells, Cultured - immunology |
| title | Cytotoxic activity of human lymphocytes against differentiated intestinal tumour cell lines |
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