Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans
C. elegans contains numerous small RNAs of ~21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-cod...
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| Veröffentlicht in: | RNA (Cambridge) 2006-04, Vol.12 (4), p.589-597 |
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| creator | Lee, Rosalind C Hammell, Christopher M Ambros, Victor |
| description | C. elegans contains numerous small RNAs of ~21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-coding genes in normal worms, are produced by mechanisms that involve DCR-1 but, unlike miRNAs, also involve RDE-2, RDE-3, RDE-4, RRF-1, and RRF-3. The tiny noncoding (tncRNAs) are similar to endo-siRNAs in their biogenesis except that they are derived from noncoding sequences. These endo-siRNA- and tncRNA-based endogenous RNAi pathways involve some components, including DCR-1 and RDE-4, that are shared with exogenous RNAi, and some components, including RRF-3 and ERI-1, that are specific to endogenous RNAi. rrf-3 and eri-1 mutants are enhanced for some silencing processes and defective for others, suggesting cross-regulatory interactions between RNAi pathways in C. elegans. Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes. |
| doi_str_mv | 10.1261/rna.2231506 |
| format | Article |
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Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes.</description><identifier>ISSN: 1355-8382</identifier><identifier>EISSN: 1469-9001</identifier><identifier>DOI: 10.1261/rna.2231506</identifier><identifier>PMID: 16489184</identifier><language>eng</language><publisher>United States: Copyright 2006 by RNA Society</publisher><subject>Animals ; Base Sequence ; Blotting, Northern ; Caenorhabditis elegans ; Caenorhabditis elegans - genetics ; Gene Silencing ; Mutation ; Oligonucleotide Probes ; RNA Interference</subject><ispartof>RNA (Cambridge), 2006-04, Vol.12 (4), p.589-597</ispartof><rights>Copyright © Copyright 2006 by RNA Society 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-ab7683d460beccd6f3b35966686f893de53321fb79ff603e4ef14c7898aba1023</citedby><cites>FETCH-LOGICAL-c476t-ab7683d460beccd6f3b35966686f893de53321fb79ff603e4ef14c7898aba1023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1421084/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1421084/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16489184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Rosalind C</creatorcontrib><creatorcontrib>Hammell, Christopher M</creatorcontrib><creatorcontrib>Ambros, Victor</creatorcontrib><title>Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans</title><title>RNA (Cambridge)</title><addtitle>RNA</addtitle><description>C. elegans contains numerous small RNAs of ~21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-coding genes in normal worms, are produced by mechanisms that involve DCR-1 but, unlike miRNAs, also involve RDE-2, RDE-3, RDE-4, RRF-1, and RRF-3. The tiny noncoding (tncRNAs) are similar to endo-siRNAs in their biogenesis except that they are derived from noncoding sequences. These endo-siRNA- and tncRNA-based endogenous RNAi pathways involve some components, including DCR-1 and RDE-4, that are shared with exogenous RNAi, and some components, including RRF-3 and ERI-1, that are specific to endogenous RNAi. rrf-3 and eri-1 mutants are enhanced for some silencing processes and defective for others, suggesting cross-regulatory interactions between RNAi pathways in C. elegans. Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Gene Silencing</subject><subject>Mutation</subject><subject>Oligonucleotide Probes</subject><subject>RNA Interference</subject><issn>1355-8382</issn><issn>1469-9001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtLAzEYDKLYWj15lz15ka15bTZ7EaT4KBQF0XPIZr9tI9tsTbZq_72Rrq-Tp-8xwzDDIHRM8JhQQc6902NKGcmw2EFDwkWRFhiT3bizLEslk3SADkJ4jk8W4X00IILLgkg-RLOp68Br01k3T8BV7Rxcuw6JdlUC71_Xw92lTVa6W7zpTUisSyY6An6hy8p2NiTQwFy7cIj2at0EOOrnCD1dXz1ObtPZ_c10cjlLDc9Fl-oyF5JVXOASjKlEzUqWFUIIKWpZsAoyxiipy7yoa4EZcKgJN7kspC41wZSN0MVWd7Uul1AZcJ3XjVp5u9R-o1pt1V_E2YWat6-KcEqw5FHgtBfw7csaQqeWNhhoGu0g5lUil5RlOf2XSDHlOIumR-hsSzS-DcFD_e2GYPVZk4o1qb6myD75HeCH2_fCPgAAM49n</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Lee, Rosalind C</creator><creator>Hammell, Christopher M</creator><creator>Ambros, Victor</creator><general>Copyright 2006 by RNA Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060401</creationdate><title>Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans</title><author>Lee, Rosalind C ; Hammell, Christopher M ; Ambros, Victor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-ab7683d460beccd6f3b35966686f893de53321fb79ff603e4ef14c7898aba1023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Gene Silencing</topic><topic>Mutation</topic><topic>Oligonucleotide Probes</topic><topic>RNA Interference</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Rosalind C</creatorcontrib><creatorcontrib>Hammell, Christopher M</creatorcontrib><creatorcontrib>Ambros, Victor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RNA (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Rosalind C</au><au>Hammell, Christopher M</au><au>Ambros, Victor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans</atitle><jtitle>RNA (Cambridge)</jtitle><addtitle>RNA</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>12</volume><issue>4</issue><spage>589</spage><epage>597</epage><pages>589-597</pages><issn>1355-8382</issn><eissn>1469-9001</eissn><abstract>C. elegans contains numerous small RNAs of ~21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-coding genes in normal worms, are produced by mechanisms that involve DCR-1 but, unlike miRNAs, also involve RDE-2, RDE-3, RDE-4, RRF-1, and RRF-3. The tiny noncoding (tncRNAs) are similar to endo-siRNAs in their biogenesis except that they are derived from noncoding sequences. These endo-siRNA- and tncRNA-based endogenous RNAi pathways involve some components, including DCR-1 and RDE-4, that are shared with exogenous RNAi, and some components, including RRF-3 and ERI-1, that are specific to endogenous RNAi. rrf-3 and eri-1 mutants are enhanced for some silencing processes and defective for others, suggesting cross-regulatory interactions between RNAi pathways in C. elegans. Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes.</abstract><cop>United States</cop><pub>Copyright 2006 by RNA Society</pub><pmid>16489184</pmid><doi>10.1261/rna.2231506</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Base Sequence Blotting, Northern Caenorhabditis elegans Caenorhabditis elegans - genetics Gene Silencing Mutation Oligonucleotide Probes RNA Interference |
| title | Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans |
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